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Biomedical subjects

K Sun

Publications and source records attributed to K Sun.

At least 145 records · Page 8Linked to original sources
PubMed1996

[Histochemical and electronic microscopical studies of stellate fibrillar formations (SFF) in salivary gland tumors].

PURPOSE: To study the structure and significance of SFF in pleomorphic adenoma and myoepithelioma. MATERIAL AND METHODS: Tow cases of each tumor were studied. Histologie and ultrathin sections were made, stained with HE, Mallory, Van Gieson, Pollak methods, and examined with light and transmission electronic microscopes. RESULTE: The SFF were proved to be formed by radial arrangement of collagen fibers, which were composed of striped fibrillae under TEM. CONCLUSION: The structure of the SFF is understood, their existence may play a role the pathologic diagnosis.

Adenoma, Pleomorphic↗
PubMed1996

[Prevention of peridural fibrosis and adhesion after laminectomy: an experimental study in rats].

An experimental study on the effectiveness of various materials including poly carboxymethylcellulose (PCMC), gelatin foam (GF), ligment (L), free fat (FF) in prevention of peridural fibrosis and adhension (PDA) was done in 144 SD rats using a total laminectomy model (L1-2). PDA was measured with a double-blind protocol at 2, 4, 8, 12 weeks postoperatively by gross anatomical appearance (blunt-dissection), microscopical evaluation, computed imaging analysis and MRI enhanced with gadolinium. The results demonstrated that the peak of PDA is within 8 weeks after operation. PCMC, as a three dimensional protective material, could effectively inhibit PDFA after laminectomy, but GF and L could not. SHA had some effect in the early stage, but not in the late period. For FF though it could effectively prevent PDA, the incision infection rate was higher, the atrophy and necrosis of FF were serious. What is more, almost all transplanted fat remained in situ extended into the spinal cord in some measure after 8 weeks postoperatively.

Animals↗
PubMed1995

Complex and rapid-cycling in bipolar children and adolescents: a preliminary study.

26 subjects aged 7-18 years were studied. Diagnoses of bipolar disorders were established using the Kiddie-Schedule for Affective Disorders and Schizophrenia-Present Episode Version-1986 modified for DSM-III-R criteria and for rating the number and duration of manic and hypomanic episodes. Complex cycling patterns were observed. These included numerous brief episodes suggesting continuous rapid-cycling in 80.8% of cases. Mean age of onset was early (8.5 +/- 4.4 years). Psychotic phenomena, suicidality, hyperactivity and 'mixed mania' were highly prevalent. Data in this report provide support for complex and rapid-cycling patterns in childhood onset bipolar disorder.

Adolescent↗
PubMed1995

Effect of acute and long-term smoking on myocardial blood flow and flow reserve.

BACKGROUND: Cigarette smoking is a major preventable risk factor for coronary artery disease and sudden cardiac death. However, the effect of acute and long-term cigarette smoking on coronary vasodilatory capacity and myocardial flow reserve has not been quantified in humans. METHODS AND RESULTS: To examine the effect of short-term and long-term smoking, myocardial blood flow was quantified at rest and during dipyridamole-induced hyperemia (0.56 mg/kg) in 12 smokers (10 males and 2 females; mean age, 27 +/- 4 years) under baseline conditions (reflecting the effect of long-term smoking) and during short-term cigarette smoking with 13N ammonia, positron emission tomography, and a two-compartment model. Twelve sex- and age-matched nonsmokers served as control subjects. Smoking significantly increased the rate-pressure product at rest from 7525 +/- 1290 to 9160 +/- 1125 (P < .001 versus baseline), which was paralleled by a proportional increase in myocardial blood flow at rest (0.70 +/- 0.17 versus 0.88 +/- 0.17 mL.g-1.min-1; P < .05 versus baseline). In contrast, hyperemic blood flow declined from 2.23 +/- 0.35 at baseline (P = NS versus control) to 1.98 +/- 0.32 mL.g-1.min-1 during smoking (P < .01 versus baseline). Accordingly, the myocardial flow reserve declined from 3.36 +/- 0.83 in smokers at baseline to only 2.28 +/- 0.28 during smoking (P < .0001 versus baseline). Thus, myocardial blood flow and flow reserve were similar in young, long-term smokers and young, healthy nonsmokers. CONCLUSIONS: Short-term smoking increases the coronary vasomotor tone during dipyridamole-induced hyperemia and markedly reduces the myocardial flow reserve. In contrast, long-term smoking does not attenuate the coronary vasodilatory capacity in young individuals with a relatively short smoking history. It might be speculated that the short-term reduction in the coronary vasodilatory capacity during smoking could lower the ischemic threshold in smokers with coronary artery disease and contribute to the increased risk for sudden cardiac death.

Adult↗
PubMed1995

Surgical management of complete atrioventricular septal defects. A twenty-year experience.

Creation of a competent left atrioventricular valve is a cornerstone in surgical repair of complete atrioventricular septal defects. To identify risk factors for mortality and failure of left atrioventricular valve repair and to determine the impact of cleft closure on postoperative atrioventricular valve function, we retrospectively analyzed hospital records of 203 patients between January 1974 and January 1995. Overall early mortality was 7.9%. Operative mortality decreased significantly over the period of the study from 19% (4/21) before 1980 to 3% (2/67) after 1990 (p = 0.03). Ten-year survival including operative mortality was 91.3% +/- 0.004% (95% confidence limit): all survivors are in New York Heart Association class I or II. Preoperative atrioventricular valve regurgitation was assessed in 203 patients by angiography or echocardiography and was trivial or mild in 103 (52%), moderate in 82 (41%), and severe in 18 (8%). Left atrioventricular valve cleft was closed in 93% (189/203) but left alone when valve leaflet tissue was inadequate and closure of the cleft might cause significant stenosis. Reoperation for severe postoperative left atrioventricular valve regurgitation was necessary in eight patients, five of whom initially did not have closure of the cleft and three of whom had cleft closure. Six patients had reoperation with annuloplasty and two patients required left atrioventricular valve replacement. Five patients survived reoperation and are currently in New York Heart Association class I or II. On most recent evaluation assessed by angiography or echocardiography (a mean of 59 months after repair), left atrioventricular valve regurgitation was trivial or mild in 137 of the 146 survivors (94%) examined; none had moderate or severe left atrioventricular valve stenosis. By multiple logistic regression analysis, strong risk factors for early death and need for reoperation included postoperative pulmonary hypertensive crisis, immediate postoperative severe left atrioventricular valve regurgitation, and double-orifice left atrioventricular valve. These results indicate that complete atrioventricular septal defects can be repaired with low mortality and good intermediate to long-term results. Routine approximation of the cleft is safe and has a low incidence of reoperation for left atrioventricular valve regurgitation.

Adolescent↗
PubMed1995

Quantitative multivariate analysis of myoepithelioma and myoepithelial carcinoma.

The nuclear DNA content and morphologic data of 30 surgical samples (eight normal parotid gland, 12 myoepithelioma, and 10 myoepithelial carcinoma) were analyzed by the IBAS II analysis system. The results suggest that there are relationships between tumor ploidy distribution and prognosis, and that correct pathologic diagnosis can be obtained by the IBAS image system.

Adolescent↗
PubMed1995

Estimation of acute toxicity by fitting a dose-time-response surface.

In acute toxicity testing, organisms are continuously exposed to progressively increasing concentrations of a chemical and deaths of test organisms are recorded at several selected times. The results of the test are traditionally summarized by a dose-response curve, and the time course of effect is usually ignored for lack of a suitable model. A model which integrates the combined effects of dose and exposure duration on response is derived from the biological mechanisms of aquatic toxicity, and a statistically efficient approach for estimating acute toxicity by fitting the proposed model is developed in this paper. The proposed procedure has been computerized as software and a typical data set is used to illustrate the theory and procedure. The new statistical technique is also tested by a data base of a variety of chemical and fish species.

Algorithms↗
PubMed1995

Vasopressin actions on area postrema neurons in vitro.

The area postrema (AP) is a circumventricular organ located on the dorsal surface of the medulla. Substantial evidence suggests that the AP is an important site involved in cardiovascular regulation. Arginine vasopressin (AVP) is thought to act at the AP to increase the sensitivity of the baroreceptor reflex. We have therefore examined the effects of AVP on AP neurons with the use of extracellular single unit recordings in vitro. Coronal medullary brain slices (thickness = 400 microns) were obtained from male Sprague-Dawley rats and maintained in oxygenated artificial cerebrospinal fluid (aCSF). The slices were perfused with AVP (10(-8) to 10(-6) M), and the effect on single AP neurons was recorded. A total of 79 AP neurons was tested of which 50 (63.3%) were excited by AVP and 5 (6.3%) were inhibited, whereas the remaining 24 (30.3%) cells were unaffected. The excitatory effects of AVP were dose dependent: firing rate increased 92.6 +/- 25.8% at 10(-8) M, 289.4 +/- 53.9% at 10(-7) M, and 456.8 +/- 113.1% at 10(-6) M, respectively. We also examined whether these effects of AVP resulted from direct actions of this peptide on AP cells by testing if responses were retained during blockade of synaptic transmission (achieved by perfusion with a low Ca(2+)-high Mg2+ aCSF) in 11 cells excited by AVP. Nine of these cells were excited by AVP during such synaptic blockade. Finally, we demonstrated that the excitatory responses of five AP cells to AVP were all totally abolished by perfusion of slices with aCSF containing the V1 antagonist ([1-beta-mercapto-beta,beta-cyclopentamethylene propionic acid,2-(O-methyl)tyrosine]-Arg8-vasopressin; Peninsula Laboratories, 10(-6) M).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

[The ultrastructure study of white sponge nevus in oral mucosa].

Biopsies from 7 patients with white sponge nevus were examined histologically. Two of them had family history. Light microscopic observation reveals that the epithelium was thickened; the spinous cell layers had enlarged and oedematous cells and ballon-like degeneration; the keratohyalin granules were increased in upper spinous cells; the superficial layers of epithelium exhibited parakeratosis and thickening. 3 specimens were examined by electron microscopy. The basal and parabasal cells were cylindrical. The cytoplasmic tonofilaments were aggregated into boundles. It was characteristics of keratinized epithelium. In middle and superficial stratum spinosum the tonofilaments were homogeneously distributed in the cell plasma and the electron-dense granulars were located near the cell boundary and attached to desmosomes by tonofilaments, unlike normal non-keratinized oral mucosa. Many Odland bodies with a lamellar structure and irregular in both size and shape were seen. It is concluded that this leasion have a dyskeratinized process. The characteristic features of keratinized oral mucosa in non-keratinized mucosa is appeared. The thickening of superficial layer is due to shedding disturbance relating to dysfunction of Odland bodies.

Biopsy↗
PubMed1995

[Histogenesis of pleomorphic adenoma of the salivary gland].

Six parotid pleomorphic adenomas were studied histochemically by electron microscopy after staining with ruthenium red. It was found that the myxoid region was filled with ruthenium red granules. Similar positive ruthenium red-stained intracellular granules were observed within the Golgi-derived vacuoles of the neoplastic myoepithelial cells in the myxoid region. Some cells located at the periphery of the double-layer ductal structure had the features of myoepithelial cell, they also showed similar positive ruthenium red-stained granules in the intracellular vacuoles and intercellular space. No ruthenium red-stained granules was obtained in neoplastic ductal epithelium and the mucus in ductal cavities were negative for ruthenium red stain. The observations indicate that the neoplastic myoepithelial cells in the myxoid region probably derive from the periphery of the double-layer ductal structure, they secrete the proteoglycans forming the myxoid region and play an important role in the histogenesis of pleomorphic adenoma.

Adenoma, Pleomorphic↗
PubMed1995

Structural and ultrastructural studies of the synovial membrane in temporomandibular joint disturbance syndrome.

To gain further knowledge on temporomandibular joint disturbance syndrome (TMJDS), the synovial membranes of 7 patients and 3 cadavers were examined with light microscope and transmission electron microscope. The results showed that the synoviocytes degenerated or disappeared in TMJDS. The cytoplasm contained numerous fibrils, while the organelles diminished in number. The surface of the synovial membrane was covered with a fibrinoid material. The surface of the synovial membrane was covered with a fibrinoid material. The authors consider that these pathological changes reduce or eliminate the normal functions of the synovial membrane, which may lead to damage to the articular cartilage. The articular adhesion found sometimes in TMJDS may be related to the changes in the synovial membrane.

Adult↗
PubMed1995

[The prognostic significance of lymph node metastasis in surgical resection of esophageal cancer].

The metastatic rate and degree of lymph node (LN) were evaluated and the relation between prognostic significance and LN metastasis (M) was analysed through a retrospective study of 474 patients undergoing resections of non-irradiated esophageal carcinoma. In patients with LNM, the LNM rate was 44.5%. A total of 5382 LNs were resected and examined pathologically. 690 of them were metastatic ones with a LNM degree of 12.8%. The 5-year survival rate of this series was 30.6% (145/474), and was 12.8% (27/211) in patients with LNM and 44.9% (118/263) in those without LNM. We concluded that surgery remains the first choice of treatment for carcinoma of esophagus, and that meticulous LN dissection is an important part of surgical oncology. This is especially true when the cancer process is reasonably localized. However, in more advanced cases of this disease, surgery alone is of limited value in eradicating all cancer compromized tissue, and therefore the routine practice of extensive LN dissection in such cases may not be rewarding.

Adult↗
PubMed1995

[Clinical study on the feasibility of preserving vagal trunks in the surgical treatment of carcinoma of the thoracic esophagus].

The results of preserving vagal trunks in the surgical treatment of 47 patients with the carcinoma of the thoracic esophagus were discussed through a controlled study. There were 20 patients in the test group with vagus nerve being preserved (PG) and 27 in the control group (CG) with vagus nerve being routinely severed. Eighteen patients in PG and 26 in CG underwent radical resections, the remaining patients received palliative surgery. Pathological findings showed that 3 cases (15.0%) in PG and 3 cases (11.1%) in CG had cancerous invasion near the vagal trunks and/or their main branchs close to the primary cancer; and 1 case (5.0%) in PG and 3 (11.1%) in CG the vagal nerves were actually invaded by cancer cells. Determinations of basal gastric acid output (BAO) and half gastric emptying time (T 1/2) revealed that the mean value of BAO (1.93mmol/h) and T 1/2(63.51min.) of the PG were statistically different from those of 0.75mmol/h and > 105min. in CG, but similar to those of the healthy group and the preoperative control values. We consider that it is feasible to preserve vagal trunks in the operation and is beneficial in minimizing of postoperative disturbances of gastric acid output and gastric motility; but the indications of this procedure should only be limited to T1-T2 lesions in order not to jeopardize the radicality of tumor resection.

Adult↗
PubMed1995

CEDIA for screening drugs of abuse in urine and the effect of adulterants.

The performance of the Microgenics CEDIA DAU assays for screening amphetamines, barbiturates, benzodiazepines, cocaine, opiates, phencylidine (PCP), and tetrahydrocannabinol (THC) was evaluated on the Boehringer Mannheim/Hitachi 717 in urine. Limits of detection ranged from 0.6 ng/mL for PCP, to 34.1 ng/mL for benzodiazepines. The average within run and total precision for these assays ranged from 1.3 to 7.3% for controls at cutoff concentrations, and control values at -25% and +25% of cutoffs. The rate separations by CEDIA between the negative and cutoff calibrators for all drugs were greater than corresponding EMIT II (Syva Co.) assays. The relative sensitivity and specificity of CEDIA as compared to EMIT II were 95.6 and 98.8%, respectively, on 13,535 urine samples. All positive samples, and those samples producing discordant results between the assays were confirmed by quantitative gas chromatography/mass spectrometry (GC/MS). Using SAMHSA cutoff limits (and including barbiturates and benzodiazepines at 300 ng/mL), the relative sensitivity and specificity of CEDIA vs. EMIT II were 96.7 and 98.8%, respectively. The overall sensitivity of CEDIA vs. GC/MS was 98.9% with 179 false positives, as compared to 96.2% with 189 false positives for EMIT II vs. GC/MS. The effect of adulterants added to urine to potentially invalidate screening results was also tested. CEDIA produced strong interferences for most drug assays in the presence of glutaraldehyde, detergent, and high concentrations of bleach and Drano. Minimal or selective interferences were seen with golden seal tea lemon juice, Visine, and low concentrations of bleach and Drano. Essentially no interference was observed with bicarbonate, sodium chloride, and vinegar.

Gas Chromatography-Mass Spectrometry↗
PubMed1995

[An electron microscopic histochemical study on proteoglycans in salivary gland myoepithelioma].

Four myoepitheliomas and four parotid gland were studied histochemically be electron microscopy after stain with ruthenium red. It was found that the normal myoepithelial cells of parotid gland had no secretion function, whereas the myoepithelioma cells were capable of secreting proteoglycans and might form the myxoid region in this tumor. The authors propose that in the pathological diagnosis and differentiat diagnosis of salivary gland epithelial tumors, the appearance of the myxoid region is not only the pathological feature of pleomorphic adenoma, but also the pathological change of myoepithelioma.

Humans↗