Search PubMed⌕ Search

Biomedical subjects

K Sugimoto

Publications and source records attributed to K Sugimoto.

At least 559 records · Page 31Linked to original sources
PubMed1987

SOS-inducing activity of chemical carcinogens and mutagens in Salmonella typhimurium TA1535/pSK1002: examination with 151 chemicals.

The umu test system is a newly developed method to evaluate genotoxic activities of a wide variety of environmental carcinogens and mutagens (Oda et al., 1985). In the present study, we further examined the abilities of 151 chemicals to induce umu gene expression in Salmonella typhimurium TA1535/pSK1002. Among the chemicals examined, 72 compounds induced umu gene expression, which could be defined on a basis of increased beta-galactosidase activity by 2-fold over the background level. The potent genotoxic compounds without metabolic activation were adriamycin, bleomycin, daunorubicin, 1,3-dinitropyrene, 1,6-dinitropyrene, 1,8-dinitropyrene, N-ethyl-N'-nitro-N-nitrosoguanidine, furylfuramide, methyl methanesulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, mitomycin C, 1-nitropyrene and 4-nitroquino-line-1-oxide. In the presence of S9, aflatoxin B1, 2-aminoanthracene, Glu-P-1, IQ, MeIQ, MeIQx, Trp-P-1 and Trp-P-2 also induced umu gene expression markedly. Several chemicals such as 2-acetylaminofluorene, 9-aminoacridine, azobenzene, benzanthracene, benzidine, diethyl nitrosamine, 1-nitronaphthalene, paraquat, potassium dichromate and sodium nitrite were weakly genotoxic and the induction by these compounds could be detected only when the incubation time was prolonged from 2 h to 5 h. Data are also presented that some of the chemicals such as dimethyl sulfoxide, m-dioxan, 5-fluorouracil and paraquat, which have been reported to be non-mutagenic in Ames/Salmonella assay, were found to be active in inducing umu gene expression, while the known mutagenic compounds including acrylonitrile, 4,4'-dinitrobiphenyl, furfural, methylene chloride, 1-naphthylamine, sodium azide, o-tolidine and o-toluidine were non-genotoxic in the present assay system.

Biotransformation↗
PubMed1987

Relative roles of T7 RNA polymerase and gene 4 primase for the initiation of T7 phage DNA replication in vivo.

Initiation sites of T7 phage DNA replication in the presence and absence of T7 phage gene 4 primase have been analyzed by using Escherichia coli cells infected with T7 phage amber mutants, T73,6 and T73,4,6, respectively. Restriction analysis of the [3H]thymidine-labeled DNA, synthesized by the T73,4,6 phage-infected cells in the presence of 2',3'-dideoxy-3'-azidothymidine, has shown that only the light (L) strand of T7 DNA has been synthesized from the primary origin area to the right. Transition sites from RNA to DNA have been located precisely in the primary origin region of the T7 phage genome. In the gene 4- condition, greater than 20 transition sites have been detected only in the L strand. They scattered widely downstream from the phi 1.1 promoters and mostly downstream from the phi 1.3 promoter. The same transition sites have been detected in the gene 4+ condition, suggesting that the transcripts started from these promoters are used as primers of the rightward L-strand DNA synthesis in the gene 4+ condition. In addition, many heavy (H)- and L-strand transition sites have been detected at gene 4 primase sites in the gene 4+ condition. The relative roles of T7 phage RNA polymerase and primase at the primary origin have been discussed.

Base Sequence↗
PubMed1987

[An interview survey on health care service in small-sized manufacturers in a city of Osaka Prefecture].

An interview survey on the health care service for workers employed in small-sized enterprises was conducted in a city of Osaka Prefecture. Items surveyed were: 1) number of full-time and part-time employees by age (less than 39, 40-60, and more than 60 yr) and sex, 2) materials and production process, 3) periodic examinations for general health status and occupational diseases, and others. Of the 526 enterprises interviewed, 459 manufacturers with less than 50 employees were analyzed. The results can be summarized as follows: 1. Of the 459 manufacturers, 72.4% were those with less than 10 workers (the mean number of employees: 8.4). The major type of manufacture was electric parts processing industry. 2. The period health examination has been carried out in 372 enterprises (81.0%). The smaller the size of the company, the lower was the rate of the periodic health examination. 3. In 77.4% of the 372 enterprises, only chest X-ray was an item for the periodic health examination, which was usually carried out at the public health center of the community. 4. The number of enterprises conducting the periodic health examination designated by the Industrial Safety and Health Law (1972) was only 49 (10.7%). 5. Fourteen enterprises (3.1%) have carried out periodic examination for occupational diseases. 6. The periodic examination for degenerative diseases such as hypertension, diabetes mellitus, and other cardiovascular diseases was carried out in 57 enterprises (12.4%). The larger the size of the company, the higher was the rate in which such examination was conducted.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1987

Serum levels of bone Gla-protein in normal humans and in patients with chronic renal failure.

Levels of serum bone Gla-protein (BGP) were evaluated in 372 normal Japanese subjects (164 males, 208 females), ranging in age from 14 to 87 years, and in 194 patients with chronic renal failure, 181 of whom were on maintenance hemodialysis. The age-dependent serum BGP levels (mean +/- SD) determined by radioimmunoassay in normal subjects under 20 years old were 27.1 +/- 20.5 ng/ml, and 10.2 +/- 5.8 ng/ml in older subjects. Mean serum BGP increased significantly in patients with hemodialysis (35.1 +/- 24.3 ng/ml) and in patients with nonhemodialysis chronic renal failure (20.6 +/- 14.2 ng/ml). The mean BGP levels showed a trend toward higher values in the younger renal failure patients. However, the difference to the values of older patients did not reach a level of statistical significance. There was a significant (r = 0.84, p less than 0.001) positive correlation between serum BGP and serum creatinine for the nonhemodialysis group alone, but not for hemodialysis patients alone. A correlation was also observed between serum BGP and serum C-PTH in hemodialysis patients. Our results suggest that the serum BGP elevation in patients with chronic renal failure probably reflects not only decreased renal clearance but also increased bone formation.

Adolescent↗
PubMed1987

[Pharmacokinetics of cis-dichlorodiammine platinum (II) in patients undergoing hemodialysis].

The pharmacokinetics of non-protein-bound platinum (free-Pt) and protein-bound platinum derived from cisplatin [cis-dichlorodiammine platinum (II), CDDP] were studied in five patients with chronic renal failure who developed gastric cancer during long-term hemodialysis. Plasma concentrations of CDDP were determined by atomic absorption spectrophotometry. CDDP at a dose of 25 mg/m2 was given i.v. over a 30-min period every one to two weeks. When CDDP was infused simultaneously with the start of hemodialysis, free-Pt at the blood inlet decreased rapidly and free-Pt at the blood outlet remained below the level for quantitative analysis. Furthermore, the total-Pt level declined in two exponential curves at the blood inlet simultaneously with the increased level of Pt at the blood outlet after the end of dialysis. In contrast, when CDDP was infused one hour earlier before dialysis, the pharmacokinetics revealed a higher concentration of free-Pt at the blood inlet which was maintained over a longer period as compared with that occurring when infused simultaneously with the start of dialysis. In conclusion it is considered that this method is feasible for the treatment of gastric cancer in patients with chronic renal failure under long-term hemodialysis.

Cisplatin↗
PubMed1986

[A questionnaire survey on the dissemination of personal exposure monitoring of organic solvents in Japan].

A questionnaire survey on the dissemination of personal exposure monitoring of hazardous chemicals, especially organic solvents, was conducted on 404 Japanese institutions for occupational health and environment, including enterprises, national and municipal institutes, universities and colleges. Responses were received from 236 organizations. The results can be summarized as follows: Of the 236 organizations which responded, 89 (37.7%) had some experience in measuring personal exposure in workers exposed to hazardous chemicals. In 74.2% of these 89 institutions, personal exposure monitoring of organic solvents was being made. Passive dosimeter method was far more commonly employed in Japan than charcoal tube method. Personal exposure measurement is predominantly regarded to be supplementary to the general air sampling and measurement method provided for in the Working Environment Measurement Law (1975). It is considered that personal exposure monitoring would be further disseminated in Japan than today with the aim of improving the health care system for industrial workers and of elucidating epidemiologically the relationship of chemical exposure to biological effects.

Air Pollutants, Occupational↗
PubMed1986

Glomerular injury in mice induced by Agkistrodon venom.

Glomerular injury was produced in mice after a single LD50 intravenous dose of purified 100-pace snake venom (Agkistrodon acutus). Characteristic features in glomeruli where the venom was demonstrated immunohistochemically included cystic lesions of the capillary tufts, thrombosis, subsequent proliferative and sclerotic changes, and crescent formation. Venom was recognized immunohistochemically in the glomerular endothelium, visceral basement membrane, mesangium, epithelium, and Bowman's capsule. Venom transfer across the basement membrane or into cells required less than 2 hours. Within this period mesangiolysis occurred due to the protease activity of the venom. Cystic lesions with fibrin thrombi then developed, which were replaced by proliferative and sclerotic changes after 3 days. Crescent formation began within 48 hours and was completed within a few days. Microangiography revealed hemodynamic changes in the intrarenal vasculature after venom treatment. Immune complex deposition was not found in any tissue. The study demonstrates the moderately constant potency (LD50) of Agkistron acutus venom, and documents it as a useful tool for quantitative studies of renal lesions.

Angiography↗