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Biomedical subjects

K Sudo

Publications and source records attributed to K Sudo.

At least 109 records · Page 6Linked to original sources
PubMed1996

[A case report of surgical management of blunt ruptured right atrium with blunt ruptured liver].

This is a case report of a 23 year old female involved in a car accident that caused blunt trauma to the patient. First of all, she was found to have cardiac tamponade when the abdomen was explored to suture and to put the gaze compression on for hemostasis of the ruptured liver. Then, she was brought to our institution by the ambulance. Upon the exploration of the heart under standby of extracorporal circulation (ECC), small multiple lacerations were found at the junction of the right atrium and superior vena cava. These were sutured directly to close without ECC. On the 2nd postoperative day, she was bought to the OR again to removal of the gaze tamponade from the ruptured liver and to complete hemostasis. The patient was discarded 35 days after admission.

Accidents, Traffic↗
PubMed1996

[Obstruction of the true lumen by retrograde perfusion during repair of DeBakey type I aortic dissection: a case report].

Retrograde femoral perfusion is often used during the repair of DeBakey type I aortic dissections, however, it may cause serious ischemic damage of vital organs even though the arterial cannula is properly placed. A 58-year-old woman with chest pain was admitted to our hospital. She was operated on urgently because chest computed tomograms revealed that she suffered from a DeBakey type I aortic dissection. A ringed graft, 22 mm in diameter, was implanted into the ascending aorta using retrograde perfusion through the right femoral artery. Following the removal of the aortic cross-clamp and rewarming, the prosthetic graft remained flaccid and the heart failed to resume beating. We speculated that retrograde femoral perfusion caused the true lumen obstruction while distending the false lumen. This resulted in blocking reperfusion of the coronary arteries. After antegrade perfusion was initiated through an 8 mm Dacron graft which was anastmosed to the ringed graft, the heart soon resumed beating. During the repair of DeBakey type I aortic dissections, this serious complication should be anticipated. If it occurs, retrograde femoral perfusion must be exchanged for antegrade aortic perfusion before irreversible changes occur.

Aortic Dissection↗
PubMed1996

Long-term effects of intensive therapy combined with tonsillectomy in patients with IgA nephropathy.

IgA nephropathy (IgAN) is the most common form of glomerular disease in the world. However, there is currently no established therapy for IgAN. To assess treatment regimens for IgAN, we investigated a retrospective long-term follow-up study comparing an intensive therapy with a conventional therapy. Clinical outcomes 5 years after the initiation of treatment in two centers were compared. In one center, patients were treated with tonsillectomy combined with steroid pulse, cyclophosphamide, anti-platelet drugs and warfarin (intensive therapy group, Group A, n = 50). In the other center patients were treated with anti-platelet drugs, warfarin or no treatment (conventional therapy group, Group B, n = 50). At the beginning of treatment, the two groups were well matched in terms of age, sex, blood pressure, urinalysis, and creatinine clearance. Five years after the initiation of treatment, proteinuria was remarkably reduced from 1.6 g/day to 0.5 g/day in Group A, whereas no significant change in proteinuria was observed in Group B. Creatinine clearance significantly improved from 77.6 ml/mm to 89.4 ml/min in Group A, whereas creatinine clearance deteriorated from 70.9 ml/min to 62.5 ml/min during 5 years in Group B. Our results indicate that early intensive therapy for IgAN is potentially of great value, and warrants close investigation.

Adult↗
PubMed1995

Isolation of 115 human chromosome 8-specific expressed-sequence tags by exon amplification.

Exon-amplification experiments were undertaken to isolate potentially transcribable sequences from cosmid clones that previously had been mapped to subchromosomal bands of human chromosome 8 by fluorescence in situ hybridization. From 253 cosmids subjected to this procedure so far, we isolated 169 fragments and confirmed that they had been derived from the original cosmid clones. Among them, 38 revealed homology to repetitive DNA sequences such as Alu and L1 elements. The other 131 were unique sequences, but of these only 115 contained discernible open reading frames. Among these 115 sequences, 15 were identical to parts of six known genes listed in the public database. On the basis of information derived from mapping the original cosmid clones, we were able to localize two of these known genes, zinc finger protein 7 and heat shock transcription factor 1, to 8q24.3. Furthermore, we have proven that some of these clones are parts of the transcribed products by an exon connection method or by isolation of a novel cDNA that is homologous to murine clathrin-associated protein. The expressed-sequence tags isolated here will be useful resources for a transcriptional map of chromosome 8 and for isolation of new genes.

Adaptor Proteins, Vesicular Transport↗
PubMed1995

Genetic basis of the silent phenotype of serum butyrylcholinesterase in three compound heterozygotes.

Three Japanese patients showed very low butyrylcholinesterase activity in their sera and appeared to be homozygous for silent genes for butyrylcholinesterase. From DNA analysis, all three patients were compound heterozygotes: GGA(Gly) to CGA(Arg) at codon 365 (G365R) and TTC(Phe) to TCC(Ser) at codon 418 (F418S) in patient 1, G365R and CGT(Arg) to TGT(Cys) at codon 515 (R515C) in patient 2 and ACT(Thr) to CCT(Pro) at codon 250 (T250P) and AGA(Arg) to TGA(Stop) at codon 465 (R465X) in patient 3. The K-variant, GCA(Ala) to ACA(Thr) at codon 539, was also found in patients 1 and 2. Simple identification methods for all the mutations were developed and applied to family analysis and control individuals. The mutant alleles (with silent gene and K-variant) were segregated as predicted by theory in pedigrees of patients 1 and 2. Four of the mutations, F418S, R515C, T250P and R465X, were initially discovered in Japan and genetic heterogeneity among the human population for the butyrylcholinesterase gene was suggested.

Alleles↗
PubMed1995

Use of lactate dehydrogenase to evaluate the anti-viral activity against influenza A virus.

The detection of lactate dehydrogenase (LDH) can be used to evaluate efficiently anti-influenza A virus agents. LDH levels in the virus-infected Madin-Darby canine kidney cell cultures were significantly higher than in controls, were in proportion to the degree of virus infection, and corresponded to a decrease in mitochondrial dehydrogenase activity as assayed using a tetrazolium colorimetric assay (MTT method). The EC50 value and cytotoxicity of ribavirin, 3-deazaguanine, pyrazofurin, and carbodine against influenza A virus as measured by the LDH detection method was equivalent to that derived by the MTT method.

Amides↗
PubMed1995

Effect of donor age on the phosphorylation of the retinoblastoma gene product in cultured human keratinocytes.

The retinoblastoma gene product (pRB) is a tumor suppressor protein. Recently, it has been shown that the phosphorylation of pRB is necessary for progression from G1 to S phase in a variety of cells. In a word, the phosphorylation of pRB accelerates cell growth. In this study, we examined the phosphorylation status of pRB in normal human keratinocytes obtained from donors aged from 1 year to 71 years. The cells of old donors (61-71 years old) presented the more dense hypophosphorylated pRB band than cells of young donors (1-19 years old). Densitometric measurement showed that 42.3% +/- 4.8% of the total pRB was hypophosphorylated in cells of young donors, while 58.1% +/- 6.9% was hypophosphorylated in those of old donors. Results indicate that more pRB is hypophosphorylated in cells of old donors. The phosphorylated status of pRB might be related to the loss of growth fraction in human keratinocytes of old donors.

Adult↗
PubMed1995

Hepatocyte growth factor stimulates liver regeneration and elevates blood protein level in normal and partially hepatectomized rats.

The effects of recombinant human hepatocyte growth factor (HGF) on liver growth and function of normal and partially hepatectomized rats have been examined. HGF was continuously administered into the jugular vein because it was rapidly eliminated from the plasma (t1/2 alpha; approximately 4.5 min) and degraded. In normal rats, the labeling index of hepatocytes was increased about 6 times by the administration of HGF. HGF also decreased the prothrombin time and increased the hepaplastin and serum albumin content. In 70%-hepatectomized rats, HGF stimulated liver regeneration and increased the level of blood proteins such as hepaplastin in a dose-dependent manner. The stimulation of serum protein level seemed to result from not only the increase of hepatic cell number but also the direct effect of HGF on the protein production in hepatocytes, because HGF rapidly enhanced the protein synthesis prior to the increase of cell number and increased the mRNA content of albumin in the liver in vivo. In addition, a combination of heparin with HGF further accelerated the effects of HGF described above, possibly due to the decrease of HGF clearance. These findings suggest that HGF accelerates both the hepatic regeneration and function in vivo, and that rhHGF is clinically expected to be a potent therapeutic agent in hepatectomy and liver injury.

Albumins↗
PubMed1995

Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.

From a human fetal-lung cDNA library, we isolated a gene, human MCM2, whose nucleotide sequence predicts a protein product homologous to the yeast nuclear proteins MCM2, MCM3, CDC21, and CDC46. Determination of cDNA sequences covering the entire coding region demonstrated an open reading frame of 1,629 nucleotides encoding 543 amino acids. Sequence comparison revealed 30-40% amino acid identity with these four yeast proteins, all of which are considered to play important roles in DNA replication. Analysis by Northern blotting revealed that the human MCM2 gene is expressed ubiquitously in normal tissues. Furthermore, we localized this gene to chromosomal bands 7q21.3-->q22.1 by fluorescence in situ hybridization.

Amino Acid Sequence↗
PubMed1995

Isolation and mapping of the human beta-signal sequence receptor gene (SSR2).

We have isolated a human cDNA clone homologous to the canine beta-signal sequence receptor gene, which codes for an endoplasmic reticulum (ER) membrane protein associated with protein translocation across the ER membrane. Northern blot analysis revealed its ubiquitous expression in all organs examined. We also localized the human beta-signal sequence receptor gene (SSR2) to chromosome bands 1q21-->q23 by fluorescence in situ hybridization.

Amino Acid Sequence↗
PubMed1995

Isolation and mapping of a human gene (PDCD2) that is highly homologous to Rp8, a rat gene associated with programmed cell death.

From a human fetal lung cDNA library we isolated and characterized a human cDNA highly homologous to Rp8, a rat gene associated with programmed cell death. This cDNA, termed PDCD2 (programmed cell death-2), contained an open reading frame of 1032 nucleotides encoding 344 amino acids; it revealed 81% identity in DNA sequence and 83% identity in amino acid sequence with rat Rp8. The gene represented by PDCD2 was expressed in all human tissues examined. We assigned the PDCD2 locus to chromosomal band 6q27 by fluorescence in situ hybridization (FISH).

Amino Acid Sequence↗
PubMed1995

Isolation, characterization and chromosomal assignment of human colligin-2 gene (CBP2).

Colligin, a collagen-binding glycoprotein localized to endoplasmic reticulum, belongs to the serpin superfamily. Colligin has been identified and cloned in a number of species including mouse, rat, chicken, and human. We have isolated and characterized a full-length human cDNA clone that encodes a 418-amino-acid peptide that is highly homologous (97% identity) to the previously reported human colligin gene, and have named this new member of the colligin family human colligin-2. The expression of the colligin-2 gene (CBP2) is ubiquitous among all normal human tissues except for brain and peripheral leukocytes. We have mapped this novel gene to chromosomes 11q13.5 by fluorescent in situ hybridization (FISH) using a cosmid clone containing the entire coding sequence and the 3' non-coding sequence.

Amino Acid Sequence↗
PubMed1995

Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2.

We report isolation of human cDNA highly homologous to murine Eif4a2, a gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome. This cDNA, which encodes a 407-amino-acid protein, belongs to a highly-conserved gene family, the DEAD-box gene family. The human homologue of Eif4a2 was expressed in all normal tissues examined, but in variable amounts, being highly expressed in skeletal muscle and ovary, and less abundantly in liver, kidney, and pancreas. Furthermore, we have localized the human EIF4A2 to chromosome 18p11.2 by fluorescent in situ hybridization.

Amino Acid Sequence↗
PubMed1995

Substitution of lysine for arginine in the N-terminal 217th amino acid residue of the H gamma II of Staphylococcal gamma-hemolysin lowers the activity of the toxin.

The staphylococcal toxin gamma-hemolysin consists of two protein components, LukF and H gamma II. Staphylococcus aureus P83 was found to have five components, LukF, LukF-PV, LukM, LukS, and H gamma II for leukocidin or gamma-hemolysin. H gamma II of S. aureus P83 was demonstrated to be a naturally-occurring analogous molecule of H gamma II [H gamma II(P83)], in which the 217th arginine residue was replaced by lysine. The H gamma II(P83) showed about 50% of the hemolytic activity of normal H gamma II in the presence of LukF.

Amino Acid Sequence↗
PubMed1995

A screening system for antiviral compounds against herpes simplex virus type 1 using the MTT method with L929 cells.

We developed the improved MTT assay system for in vitro evaluation of anti-HSV-1 agents using L929 cells derived from mouse connective tissue. This assay system provides results in 4 days using a 96-well microplate. The EC50 values of several anti-HSV agents (ACV, BVaraU, and others) were found to be similar to those obtained by the plaque reduction method using MRC-5 cells. The present MTT assay is rapid, accurate, and may be useful as an automatic screening system for evaluation of anti-HSV-1 compounds.

Animals↗
PubMed1995

[Enzyme kinetics for enzyme immunoassay].

The rate of reaction involving an enzyme is greatly influenced by temperature, pH, concentration of the substrate, and a number of other factors. Therefore, an accurate measurement of enzyme-catalyzed reaction is important for enzyme immunoassay. The substrate concentration needed for half-maximum velocity (1/2 Vmax) is called the Km value (Michaelis constant) and is expressed in units of substrate concentration (moles per liter or M). Km may be considered an approximate measure of affinity of an enzyme for its substrate: the lower the Km, the higher is the affinity. At times, optimum conditions cannot be used, and compromises in optimum assay conditions must be made.

Binding, Competitive↗