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Biomedical subjects

K Sudo

Publications and source records attributed to K Sudo.

At least 325 records · Page 18Linked to original sources
PubMed1981

5alpha-reduction of an anti-androgen TSAA-291, 16beta-ethyl-17beta-hydroxy-4-estren-3-one, by nuclear 5alpha-reductase in rat prostates.

Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5alpha-dihydrostestosterone formation from 3H-testosterone was inhibited in a competitive manner by the anti-androgen. In the in vitro experiment using 3H-TSAA-291, 5alpha-reduction of the anti-androgen occurred. One, 2 and 4 hr after an intravenous administration of 140 muCi/rat of 3H-TSAA-291 to castrated rats, the unchanged TSAA-291 accumulated in higher amounts in the ventral prostate than in the plasma, skeletal muscle and levator ani muscle, thereby indicating the selective uptake of the anti-androgen by the androgen target organ. No appreciable amounts of the 5alpha-reduced metabolite of TSAA-291 were detected in the prostate, thus suggesting that TSAA-291 itself may be responsible for the anti-androgenic properties. The inhibitory potency of the 5alpha-reductase activity of several other 16beta-substituted androstane and estrane analogues was also examined.

5-alpha Reductase Inhibitors↗
PubMed1981

Bivariate ratio monitoring in clinical laboratories.

The usefulness of monitoring bivariates was studied and discussed. Analysis of the correlation between bivariates was studied and discussed. Analysis of the correlation between bivariates demonstrated that combinations of bivariates can be divided into two groups. The first group was the combination in which a close correlation between two variates could be observed in any pathophysiologic state. The monitoring of the ratio of this first group was effective for the detection of analytical mistakes and the detection of symptom poor hereditary disorders, such as pharmacogenetical disorders. The second group was one in which a high correlation between two variates was obtained in some specific pathologic stage, and the monitoring the ratio of this second group gave high predictive values for the screening of that pathologic stage. It was demonstrated that the use of bivariate monitoring was not only very simple but also provided an effective laboratory data analysis system for the laboratory routine.

Aspartate Aminotransferases↗
PubMed1981

Disposition of timiperone, 4'-fluoro-4-[4-(2-thioxo-1-benzimidazolinyl)piperidino]butyrophenone, a neuroleptic, in the rat on repeated oral dosage.

1. The metabolic disposition of [14C]timiperone was studied in male Wistar strain rats receiving an oral dose of 0.5 mg/kg consecutively daily for 21 days. 2. Blood concentrations of 14C indicate that absorption of [14C]timiperone on repeated dosage may be similar to that after a single dose. 3. Tissue levels of 14C after repeated doses were increased to up to double those after a single dose, but the decline of tissue concentrations after the 21st dose was similar to that after a single dose. Thus, no rat tissue show accumulation. 4. Repeated administration did not affect the metabolism of timiperone. 5. Excretion of 14C on the third day of repeated medication was urine, 44.7% and faeces 52.4% of the cumulative dose, and total daily excretions were more than approx. 97% of the cumulative dose in days 3-21 of the repeat dosage schedule.

Administration, Oral↗
PubMed1980

Hereditary deficiency of lactate dehydrogenase M-subunit.

A family with a complete deficiency of lactate dehydrogenase M-subunit was investigated. The propositus was an 18-year-old male who complained of exertional pigmenturia and easy fatigue. Marked discrepancy was observed in the ratio between creatine kinase and lactate dehydrogenase (CK/LDH). Electrophoretic analysis of serum LDH isoenzymes of the propositus demonstrated only one activity band of LDH H4. A complete lack of the LDH M-subunit was similarly demonstrated in erythrocytes, leukocytes and in the intermediate vastus muscle. LDH levels in the muscle specimen were markedly decreased in the patient, whereas CK and aspartate aminotransferase were almost the same as in a control subject. LDH isoenzymes of erythrocytes were analyzed in 5 siblings and in the parents. This demonstrated a complete lack of LDH M-subunit in 3 siblings. The ratio between H-subunit and M-subunit (H/M) in erythrocyte LDH suggested a partial absence of the M-subunit in two siblings and in the parents. An abortive increase of blood lactate and a marked increase in blood pyruvate were observed immediately after ischemic work of the forearm, accompanied by an increase in serum creatine kinase and myoglobinuria. The present case represents a newly described form of genetically determined myopathy.

Adolescent↗
PubMed1980

Membrane associated enzyme complexes containing lambda type immunoglobulin A.

The properties of the membrane associated enzyme complexes were investigated. The enzyme complexes of alkaline phosphatase, arylamidase, and L-gamma-glutamyltranspeptidase were excluded by gel filtration on Sephadex G-200. The electrophoretic mobility of the enzyme complexes was found to be in the alpha-1 portion and these enzyme activities were immunologically fixed by anti-IgA and anti-light chain lambda antibodies. Heterogeneity in density was observed by density gradient ultracentrifugation. The activities of membrane-associated enzymes were demonstrated in both high (d > 1.21) and low density particles (1.063 < d < 1.21), and the enzyme activities of a high density particle were immunologically fixed by anti-IgA and anti-light chain lambda antibodies. The heterogeneity in density was based on the presence of immunoglobulin A molecule in the complexes. The specificity of the immunoglobulin which was found in the enzyme complexes proved to be exclusively of the alpha heavy chain classes and lambda light chain types respectively. This isotype specificity of immunoglobulin A is similar to the specificity observed in enzyme-linked immunoglobulins, and simple non-specific binding of immunoglobulin and membrane moiety was eliminated. This isotype specificity is of interest in connection with the properties of the circulating autoantibodies.

Adult↗
PubMed1980

Immunological properties of athymic nude mice born from homozygous (nu/nu) parents.

No significant differences were observed between nude mice (nu/nu) born of homozygous (nu/nu) parents and those born of heterozygous (nu/+) mothers in the percentage of Thy-1-positive cells or immunoglobulin (Ig)-bearing cells in the spleen as assessed by immunofluorescence. There were no significant differences in the antibody response to thymus-dependent antigen or in the number of background plaque-forming cells to this antigen. The antibody response to thymus-independent antigen was not imparied at all in mice born from homozygous parents as compared with other nude mice or euthymic mice. It was concluded that the Thy-1-bearing (T) cells observed in nu/nu mice and the capacity of these mice to respond to thymus-dependent antigens arose independently of maternal T cells and of the influence of the maternal thymus.

Animals↗