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Biomedical subjects

K Sudo

Publications and source records attributed to K Sudo.

At least 235 records · Page 13Linked to original sources
PubMed1987

Quantitation of C3 nephritic factor of alternative complement pathway by an enzyme-linked immunosorbent assay.

We have developed an enzyme-linked immunosorbent assay (ELISA) for the quantitation of C3 nephritic factor of the alternative pathway of complement (NeFA). Incubation of the NeFA-positive serum (patient KS serum) with normal human serum (NHS) in Mg-EGTA resulted in the formation of C3-B-IgG complex. No complex was formed in EDTA. At first this was detected as three types of complexes: C3-IgG, B-IgG and B-C3, by the combination of antibodies. The reaction mixture in Mg-EGTA was filtered through an ACA 22 column, from which the complexes were eluted in the same part as the first protein peak. When IgG purified from KS serum was incubated with NHS in Mg-EGTA, B-C3 complex increased in proportion to the dose of IgG. These results indicated that only one kind of complex consisting of IgG, C3 and B (IgG-C3-B) was generated by the addition of NeFA to NHS. Serum NeFA could be quantified as the titer of B-C3 complex formed after its incubation with NHS in Mg-EGTA. Using the ELISA method, NeFA was positive in five out of six patients with membranoproliferative glomerulonephritis (MPGN) type II and in only one of 17 with MPGN type I. Titers obtained by the new method were in good accordance with those by C3 conversion and C3bBb stabilization assays for NeFA, and the new method was more exact and simple than the conventional methods.

Antigen-Antibody Complex↗
PubMed1987

Transcription termination and RNA processing in the 3'-end spacer of mouse ribosomal RNA genes.

The 3' termini of ribosomal RNA precursors from mouse FM3A cultured cells are mapped to eight sites within 625 bp downstream from the 3' terminus of 28 S rRNA. Three additional sites are mapped in liver RNA from C3H/He strain mice. Two of them, the sites at 570 bp and 625 bp are assumed to be termination sites in vivo, because they correspond to in vitro termination sites of RNA polymerase I, and 45 S RNAs having these 3' termini decay with kinetics distinct from others. The amount of 45 S RNA having the 3' terminus at other sites is variable among several mouse strains, despite their having the same DNA sequence in these regions. The ability to produce 3' termini in these sites seems to follow Mendel's law of inheritance. Therefore, we postulate that these nine sites are RNA processing sites which are controlled genetically.

Animals↗
PubMed1987

Changes in cerebral microvasculature in congenital hydrocephalus of the inbred rat LEW/Jms: light and electron microscopic examination.

The vascular apparatus in congenitally hydrocephalic rat brains was studied. The characteristic findings were structural changes in the small vessels with formation of an intracerebral cavity in the periventricular white matter. Stenotic or occluded blood vessels were located in an edematous area adjacent to the dilated lateral ventricles. Electron microscopically, the endothelial cells of capillaries and venules in the periventricular edematous region showed an irregular surface with indentations, and contained numerous plasmalemmal vesicles. Occluded capillaries were found in the border zone between the edematous and nonedematous area. These vascular changes indicate the presence of a disturbed microcirculation in the hydrocephalic brain.

Animals↗
PubMed1987

The effect of cardiopulmonary bypass on T cells and their subpopulations.

To investigate the effect of cardiopulmonary bypass (CPB) on T cells, lymphocyte subsets of peripheral blood and lymphoid organs were monitored during and after open-heart surgery (Group 1). As a control, lymphocyte subsets of peripheral blood were measured in patients undergoing thoracovascular operations without CPB (Group 2). In Group 1, analysis of each subset-to-total lymphocyte ratio revealed that observed lymphocytopenia in the early postoperative days was mainly the result of T cell reduction, and that the decrease of helper/inducer T cells contributed to this decrease. In contrast, no significant fluctuation of any lymphocyte subpopulation ratio was observed in Group 2. Analysis of lymphocyte subpopulation ratios in lymphoid organs showed that reciprocal changes of T cells and their subsets were observed in the bone marrow, thus indicating that the redistribution of T cells (especially of helper/inducer cells) seems to occur between peripheral blood and bone marrow in Group 1. Furthermore, there was no relationship between serum cortisol levels and the changes in lymphocyte subset ratios in Group 1 patients.

B-Lymphocytes↗
PubMed1987

Syphilitic myelitis with its magnetic resonance imaging (MRI) verification and successful treatment.

A 31-year-old man with a 10-day duration of a progressive paraparesis and sensory disturbance was found to have a syphilitic infection which was confirmed by the blood and cerebrospinal fluid (CSF) syphilitic reaction. A discrete and wedge-shaped Gadolinium-DTPA-enhanced lesion on magnetic resonance imaging (MRI) was identified at the level of Th 3/4. The neurologic states and MRI findings improved noticeably after a 16-day course of penicillin with a high dose of prednisolone. Therefore, we want to emphasize the importance and usefulness of MRI in syphilitic myelitis.

Adult↗
PubMed1987

Transforming genes in human hepatomas detected by a tumorigenicity assay.

Two transforming sequences in human hepatomas were detected by means of a tumorigenicity assay in nude mice using transfected NIH3T3 cells. Through hybridization with known oncogene probes, the transforming gene in one hepatoma was found to be the human hst gene. The transforming sequence in the other hepatoma showed no sequence homology with any of the oncogenes examined.

Animals↗
PubMed1987

Improvement of transplantability of human neoplastic cells to nude mice.

It was demonstrated from these results that both splenectomy and irradiation of the nude mice are effective procedures for improving the rate of successful xenotransplantation with nude mice. When there is only a small number of cells in the transplant material, applying only one treatment of either splenectomy or irradiation is not sufficient to effectively improve the rate of successful transplantation. A combination of both treatments was more effective for increasing the successful rate of transplantation. The optimal conditions for maximizing the successful transplantation rate from this study were splenectomy on the 4th day of age and irradiation and transplantation on the 35th day of age for the xenotransplantation of a small number of neoplastic cells.

Animals↗
PubMed1987

Terminal complement complex in plasma from patients with systemic lupus erythematosus and other glomerular diseases.

To evaluate terminal complement pathway activation in plasma from patients with systemic lupus erythematosus (SLE) and primary glomerular diseases, we developed an enzyme-linked immunosorbent assay (ELISA) for measuring the terminal complement complexes (TCC). The method is based on a sandwich technique using rabbit antibodies against native human C5, C7 and C9. To avoid interference by native components, we equilibrated plasma specimens with 5% polyethylene glycol buffer. The precipitates were measured by ELISA. TCC was detectable in all 14 normal controls (0.48 +/- 0.06 AU/ml; mean +/- s.e.m.). TCC levels were elevated in 18 of 54 patients with SLE (0.89 +/- 0.07 AU/ml; P less than 0.01) and in eight of 11 patients with membranoproliferative glomerulonephritis (MPGN) (3.15 +/- 0.62 AU/ml; P less than 0.01). However, only one of six patients with membranous nephropathy and none of 13 with mesangial proliferative glomerulonephritis showed high values. In SLE, TCC was correlated with circulating immune complexes and inversely with CH50, C3, C4, C5 and alternative complement pathway activity (AH50), and showed significantly high values even in normal CH50 cases (n = 34; P less than 0.01). In MPGN, TCC was inversely correlated with CH50, AH50, C3, C5 and C9. These results suggest that the classical pathway plays an important role for TCC generation in SLE and that the alternative pathway does in MPGN.

Adolescent↗
PubMed1986

A case of rheumatoid arthritis with various enzyme-immunoglobulin complexes.

Several complexes of enzymes and immunoglobulins were detected at the same time in the serum of a patient with seropositive rheumatoid arthritis. The enzymes which were found to complex with immunoglobulins were lactate dehydrogenase, alkaline phosphatase (liver, bone, placental, and intestinal isoenzymes), amylase, cytosol leucine aminopeptidase, and microsomal leucine aminopeptidase. Reconstitution studies after papain or pepsin digestion showed that the association site of the enzymes was in the Fab portion of the immunoglobulin component. We found these complexes to be the result of an antibody-antigen reaction. The binding capacity of each enzyme was variable over the entire time course. The patient's immunoglobulins, complexed with lactate dehydrogenase or amylase, could not be reconstituted using alkaline phosphatase, cytosol leucine aminopeptidase, or microsomal leucine aminopeptidase in vitro. Therefore, this suggests that the immunoglobulins which bind to each enzyme are independent of each other.

Aged↗
PubMed1986

Arteriovenous fistula following central venous catheterization.

We surgically obliterated arteriovenous fistulas in three cases that developed after subclavian catheterization. The first patient presented with heart failure two years after the catheterization, and the other two presented with asymptomatic continuous bruits. The intervals between the removal of the catheter and the appearance of the bruit were 1.5 years, three days, and two months. It took another six months for the appearance of heart failure in the first case. The feeder of the fistula was a branch of the subclavian artery in all cases. In previously reported cases, there was usually some interval between the removal of the catheter and detection of the fistula, and the feeder was much more commonly one of the branches of the subclavian artery than the subclavian artery itself.

Adult↗
PubMed1986

Detection of lactate dehydrogenase B4 in human hemolysate of patients deficient in lactate dehydrogenase B subunit activity using enzyme immunoassay.

We developed a sensitive enzyme immunoassay system specific for human lactate dehydrogenase (LDH)-B4 with antiacetylated LDH-B4 Fab'-horseradish peroxidase conjugate. The enzyme immunoassay system was not interfered with by up to 0.3 mg/tube of hemoglobin. Thus, we measured LDH-B4 concentrations in the hemolysate of seven heterozygous individuals deficient in LDH-B subunit activity and eight normal individuals. We could not find a significant difference between the LDH-B4 concentrations in heterozygous and those in normal individuals. These results demonstrate that heterozygous individuals deficient in LDH-B subunit activity produce enzymatically inactive B subunits.

Aspartate Aminotransferases↗