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Biomedical subjects

K Singh

Publications and source records attributed to K Singh.

At least 613 records · Page 34Linked to original sources

Studies on glutamic-oxalacetic (GOT) and glutamic-pyruvic (GPT) transaminases of swine kidney worm Stephanurus dentatus (Diesing, 1839). I. Assay and general properties.

Biochemical studies on the two transaminases GOT and GPT of swine kidney worm Stephanurus dentatus have been made. GOT has been found much more active than GPT. Enzyme activities are based on the formation of oxaloacetate (GOT) or pyruvate (GPT) from aspartic acid and alanine respectively with oxoglutarate. A linear relationship is observed between the enzyme concentration and activity. GOT shows a maximum activity at pH 8.0 and Michaelis constant 9 X 10(-3) M for male and 2.9 X 10(-3) M for female. GPT has an optimum pH of 7.5 and a Michaelis constant 19 X 10(-3) M for male and 8 X 10(-3) M for female. The optimum temperature for both GOT and GPT was 60 degrees C.

Alanine Transaminase↗

Haematology of the common Indian frog Rana tigrina I. erythrocytes.

A study of erythrocyte count and size in Rana tigrina has been made over a period of two years. The erythrocyte count exhibits a wide range showing a month to month variation in both the sexes. An average count of 15,93,600 in females and 10,29,700 per mm3 in males is recorded. These values are appreciably high from March to July with a peak in May and June for males and females respectively. The starvation brings about a decrease in counts generally from 12th day onwards. The size of erythrocytes averages 20.7 X 13.4 micrometer in length and width. No difference in size was observed in respect of sex or season.

Animals↗

Adenoacanthoma of lacrimal sac.

A case of adenoacanthoma of the lacrimal sac is presented. The problems of the site of origin and change of behavior of the tumor is discussed. Radical surgery with subsequent radiotherapy is advocated at an early stage to avoid local recurrence. Topical application of a chemotherapeutic agent may be worthwhile in small local recurrence.

Adenocarcinoma↗

Hematology of the common Indian Frog Rana tigrina. II. Leucocytes.

A study extending over a period of two years has been made on Leucocytes differential count in common Indian frog R. tigrina. As the values of differential count are almost similar in males and females, the data obtained for the two sexes have been pooled together. A table is given to compare the values of different Leucocytes in R. tigrina with the values reported in other species of Rana. The month to month variation has been recorded in various counts and an inverse relationship is observed in the number of Lymphocytes and Neutrophils in different months of the year.

Animals↗

Biochemical and histochemical studies on non-specific phosphomonoesterases of swine kidney worm Stephanurus dentatus (Diesing, 1839).

Biochemical and histochemical studies have been made on non-specific acid and alkaline phosphomonoesterases of S. dentatus. The two forms of acid phosphomonoesterases have been found active at pH 4.0 and 6.0. The pH optima for the two forms of aklaline phosphomonoesterases lie at 8.0 and 10.0. Studies on the distribution of acid and alkaline phosphomonoesterases in various tissues have revealed an abundance of acid phosphomonoesterase in various parts of the alimentary canal and various organs of the reproductive system. The excretory ducts show alkaline phosphomonoesterase activity only.

Acid Phosphatase↗

An unusual complication of pelvic exenteration.

A case of recurrent carcinoma at the site of a urinary conduit stoma after total pelvic exenteration for carcinoma of the cervix is reported. Treatment by radical excision of the abdominal wall encircling the conduit and postoperative radiotherapy was successful. The patient is free of disease 6 years later.

Carcinoma, Squamous Cell↗

The amoebocytic corpuscles in the circulating fluid of the lamelli-branches, Indonaia caerulia and Parreysia (Parreysia) favidens.

The circulating amoebocytes of the freshwater mussels, Indonaia caerulia and Parreysia favidens have been studied. The total number of amoebocytes is low and variable, and the individual to individual variation in total counts is particularly significant. The amoebocytes are of 3 main types: Acidophils, Large Basophils and Small Basophils. Acidophils contain large acidophilic granules and basophils fine basophilic granules within the cytoplasm. Acidophils are the largest and small basophils the smallest. The greater volume of the cell is occupied by cytoplasm in acidophils and large basophils and by the nucleus in small basophils. Large basophils are the most and small basophils the least abundant. Amoebocytes clump together by means of filipodia. Large basophils possess these protoplasmic processes in the greatest abundance, and partake most actively and in largest numbers in clumping; while small basophils lack filipodia and play an absolutely passive role. Clumping progressively becomes more extensive with passage of time following cardiac injury inflicted during blood collection. I. caerulia appears to be generally more efficient than P. favidens with regard to clumping reaction. The different structural, numerical and behavioural aspects of the amoebocytes have been considered with reference to their probable causative factors and functional import.

Animals↗

Incoherent bar images formed by a polarizing microscope with crossed polarizers.

Diffraction images of incoherent bar objects formed by a polarizing microscope with crossed polarizers are studied. Expression for intensity distribution has been derived and the results in the form of curves have been plotted. The images which are of unusual shape, different from the patterns formed by the ordinary microscope are strongly dependent upon the size of the bar/coherence parameter. The line spread function can be obtained by taking a very narrow bar.

False Positive Reactions↗