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PubMed1969

On the two deoxyribonucleases, K1 and K2, isolated from mycelia of Aspergillus oryzae. IV. Isolation of thymidylic acid rich fragments from DNA of bacteriophage f1 by digestion with DNase K2.

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Amino Acid Sequence↗

PubMed1965

Responses of the isolated ileum of the morphine-tolerant guinea pig.

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Acetylcholine↗

PubMed1965

[Studies on analgesics. IV. Analgesic actions of 1,4-bis-(phenoxyacetyl)piperazine and its derivatives].

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Analgesics↗

PubMedDate not supplied

Construction and propagation of deletion derivatives of staphylococcal tetracycline-resistance plasmid pTP-5 in Bacillus subtilis.

Tetracycline-resistance (TCr) plasmid pTP-5 (4.46 kb) from Staphylococcus aureus was cleaved with HindIII into three fragments: A (2.35 kb), B (1.57 kb) and C (0.54 kb). A deletion plasmid (3.92 kb) lacking HindIII-C fragment was obtained, and was designated pNS1. This plasmid retained the TCr phenotype and the ability to replicate autonomously in Bacillus subtilis. A restriction endonuclease cleavage map of pNS1 was constructed. Attempts to construct smaller plasmids by digesting pNS1 with BAL31 nuclease led to production of a set of deletion derivatives whose molecular sizes range from 3.75 to 2.77 kb. Through analyses of these derivatives, the regions essential for autonomous replication and expression of TCr were deduced.

Bacillus subtilis↗