Biomedical subjects

K Shiromizu

Publications and source records attributed to K Shiromizu.

58 records · Page 4Linked to original sources

PubMedDate not supplied

Evaluation of apoptosis in human endometrial adenocarcinoma: comparison of nick end labeling and Le(y) antigen immunostaining method.

In situ estimation of DNA fragmentation by the nick end labeling method and immunohistochemical detection of cell surface carbohydrate Le(y) were performed to establish correlation between the results of these two apoptosis-detecting techniques in human solid tumor tissues. Formalin-fixed and paraffin-embedded tissue samples from 10 cases of human endometrial adenocarcinoma were examined and compared by using the two techniques. A substantial fraction of carcinoma cells was found to be definitely stained by both methods, although Le(y) expression did not seem to be positively correlated with DNA fragmentation in the carcinoma tissue. These results suggest that Le(y) expression does not necessarily reflect apoptotic DNA fragmentation, at least in human endometrial adenocarcinomas, and simultaneous application of DNA nick end labeling and Le(y) immunostaining is necessary to show the relevant signs of apoptosis in histologic slides, especially gynecological cancers.

DNA Fragmentation↗

PubMedDate not supplied

Intramural carcinomas of the uterine corpus: a clinicopathological study.

Cases in which no sizable amount of tumor was observed in the uterine cavity and the main foci of the carcinoma were exclusively confined to the myometrium, were studied pathologically in a series of carcinomas of the uterine corpus. Ten out of 350 carcinomas of the uterine corpus proper fell into this category, and one case of suspicious metastatic corpus tumor from the ovary was also considered. The growth pattern of these tumors was classified into following 6 groups: (a) growth confined to the myometrium, (b) nodular growth in the myometrium with minimal endometrial involvement, (c) scattered (scirrhous) growth in the myometrium with marked shedding of the endometrium, (d) almost complete replacement of the myometrium by carcinoma with endometrial shedding (e) extreme subtype of usual endophytic growth pattern and (f) diffuse, intra-lymphatic spread of secondary tumor. One, 2, 3, 1, 3, and 1 cases of tumors of group, (a)-(f), were observed respectively in our pathological files. A spectrum of initial symptoms, diagnostic procedures, histology and histogenesis of the tumor was described in this report.

Adenocarcinoma, Clear Cell↗

PubMedDate not supplied

Occurrence of apoptotic DNA fragmentation in quiescent and proliferating cells in human endometrial adenocarcinoma tissues and the influence of apoptosis-suppressing effects of bcl-2 products.

In situ estimation of DNA fragmentation by the nick end labelling (NEL) method, and immunohistochemical examination of Ki-67 proliferative antigen and bcl-2 products in human endometrial adenocarcinoma tissues were performed to provide answers to the following two questions; a) does apoptotic DNA fragmentation occur specifically in quiescent cells or in proliferating cells or randomly in both?, b) does the bcl-2 product exert its apoptosis-suppressing effects differentially on carcinoma cells depending on their cell cycle condition?. Serial sections, one micrometer in thickness, from formalin-fixed and paraffinembedded tissues of 9 cases of human endometrial adenocarcinoma were examined. Apoptotic DNA fragmentation was observed in both quiescent (Ki-67 negative) and proliferating (Ki-67 positive) cells. Bcl-2 product-positive tumor cell islands tended to be NEL negative, although a few but non-negligible number of carcinoma cells, including both Ki-67 positive and negative ones, were NEL positive. These results indicate that, at least in human endometrial adenocarcinomas, apoptotic DNA fragmentation and bcl-2 product-independent (DNA) fragmentation occurs non-specifically with respect to the cell proliferation status. Further, the results suggest an altered regulation of cell death processes in human solid tumor tissue in vivo.

Aged↗

PubMedDate not supplied

Inverse correlation between bcl-2 expression and cell growth fraction in human endometrial adenocarcinoma tissue.

Correlation between expression status of bcl-2 products and cell growth fraction (estimated by immunostaining for Ki-67 antigen) was analyzed in human endometrial adenocarcinoma tissue in situ. For this, serial sections, 2 micrometers thick, from formalin-fixed and paraffin-embedded tissue samples of 10 cases of the carcinoma were examined. Nine out of 10 carcinomas contained both bcl-2 positive and negative nests when examined immunohistochemically. In the remaining one case, no positive reaction for bcl-2 was observed. In general, bcl-2 positive nests tended to contain quiescent (Ki-67 negative) carcinoma cells, and bcl-2 negative nests, on the contrary, a large fraction of proliferating (Ki-67 positive) cells. However, this correlation was not strict, and in a few nests, the level of growth fraction was observed to be similar irrespective of bcl-2 positivity. These results show the complex function(s) of bcl-2 products, when considering their apoptosis-suppressing effects, cell cycle dependence and influence on cell proliferation status. Further, the results suggest an altered regulation of oncogene products in human solid tumor tissue in vivo.

Adenocarcinoma↗