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Biomedical subjects

K Shima

Publications and source records attributed to K Shima.

At least 397 records · Page 22Linked to original sources
PubMed1984

Human muscle carbonic anhydrase III (CA-III). Purification, immunohistochemical localization in the human skeletal muscle and its clinical application to the neuromuscular disease.

The aim of the present work was to investigate histological localization of newly found human muscle CA-III and its diagnostic value in neuromuscular diseases. The following results were obtained. CA-III was purified as a single band from human skeletal muscle nd specific anti-CA-III antiserum was raised in the rabbits. By the direct immunoperoxidase method in human biceps muscle, CA-III was localized mainly in Type I fibers (red muscle type). A radioimmunoassay was developed for CA-III which can detect 5 ng/ml of sample. Among several human tissues, CA-III was found virtually specific to the skeletal muscles with a level of 5 mg/gm wet tissue. Normal serum CA-III level (n = 20) was 22.5 +/- 15.3 (SD) ng/ml. Among 140 cases of various diseases, elevated serum CA-III levels were found in 29 cases, all of which were only from neuromuscular diseases including 17 various muscular dystrophies, 5 polymyositis, 2 other myopathies and 5 ALS. In acute myocardial infarction with highly elevated CPK, serum CA-III remained normal. In 60 cases of various neuromuscular diseases, serum CA-III, CPK and aldolase were measured. Order of sensitivity (% frequency of elevated serum level) was CPK greater than CA-III greater than aldolase, however, CA-III was most frequently elevated in myotonic dystrophy which predominantly affects Type I fibers. In 15 ALS, raised CA-III was found in 5 cases, which were all in relatively early stages showing rapidly progressive clinical courses. This result raises a possibility to use serum CA-III for evaluation of the prognosis in ALS. It is concluded that CA-III is clinically applicable as a new diagnostic marker for muscle diseases, and probably reflects Type-I fiber abnormalities more sensitively than CPK and aldolase.

Carbonic Anhydrases↗
PubMed1984

[A case of amaurosis fugax with anomalous origin of the ophthalmic artery and atheromatous stenosis of the external carotid artery].

Amaurosis fugax associated with anomalous origin of the ophthalmic artery without occlusion of internal carotid artery is very rare. We are reporting such a case of amaurosis fugax with anomalous origin of the ophthalmic artery and atheromatous stenosis of the external carotid artery. A 49-year-old-man was referred to our neurosurgical clinic because of transient monocular blindness. The patient was diagnosed as carotid TIA and was treated within aspirin and dipyridamole. In spite of this treatment, he experienced further transient dimness of vision in the right eye, several times during a period of one month. On admission, general, physical and neurological examinations disclosed no abnormality except for a bruit over the right carotid artery. Platelet adhesiveness and aggregation were slightly elevated. Right common carotid angiogram showed marked stenosis of the origin of the external carotid artery and small "sail-like" plaque in the internal carotid sinus. The angiogram also demonstrated that the right ophthalmic artery was originated from the middle meningeal artery through the superior orbital fissure. Since choroid crescent was observed, all the branches of the ophthalmic artery including the central retinal artery were filled by the external carotid system. The right ophthalmic artery was never visualized by the selective internal carotid angiography. Subsequently right carotid endarterectomy was performed without complication. One year later he remains asymptomatic.(ABSTRACT TRUNCATED AT 250 WORDS)

Blindness↗
PubMed1984

Surgical management of piriform sinus fistula.

Four cases of acute cervical abscess resulting from infection through the left piriform sinus fistula are described. The fistula seems to be related to the cause of infection in many of previously reported cases of acute suppurative thyroiditis or "recurrent lateral cervical fistula." Complete removal of the fistula is essential to a permanent cure, and the guide of a Fogarty catheter through the fistula is useful at operation for patients with repeated infection.

Abscess↗
PubMed1984

[Low energy tail spectra of characteristic X-rays detected by a Si(Li) X-ray detector].

Characteristic X-ray spectra are inherently accompanied by the tail spectra in the low energy side when X-rays are detected with a semiconductor X-ray detector. The tail spectra of Mg, Al, Si, P, S, Cl, K, Ca, Ti, Cr and Mn KX-rays have been observed using two different Si(Li) X-ray detectors. An annular source of 55Fe has been used to excite the Mg (1.25 keV) to Ti (4.5 keV) KX-rays, and 54Mn and 55Fe sources have been used for the detection of Cr (5.4 keV) and Mn (5.9 keV) KX-rays, respectively. Observed intensity ratios of the tail area Nt to the Gaussian X-ray peak area NP have exhibited to change remarkably at the Si-K adsorption edge energy 1.84 ke V. When X-ray spectra detected with different Si(Li) detectors are compared at some specific characteristic X-ray, different values of Nt/Np intensity ratios as well as different line shapes of tail spectra have been observed. Using a simple model, the thickness of Si layer which generates the tail spectrum has been estimated, i.e., the thicknesses are about 0.05 micron for one detector and 0.09 micron for the other detector. The generation of the tail spectrum is known to be partially due to the escape effect of photoelectrons or Auger electrons from the intrinsic region.(ABSTRACT TRUNCATED AT 250 WORDS)

Diffusion↗
PubMed1984

[Studies on tissue concentrations of cefoperazone and piperacillin classified by the site of the wall of gallbladder and concentration of piperacillin in the bile after operation].

In selection of drugs to be used in the treatment of biliary tract infections, sensitivity of causative organisms to drugs and tissue concentrations of the drugs constitute important factors. In the present study we treated patients with cholelithiasis with cefoperazone (CPZ) and piperacillin (PIPC), both of which have been reported to show high concentrations in the bile, and concentrations of the drugs in serum, cholecystic bile and various sites of the wall of gallbladder were determined. In addition the drug concentrations in the serum and bile at the 1st and 7th postoperative days were also determined. The following results were obtained. Comparison of concentrations of CPZ and PIPC at various sites of the wall of gallbladder revealed that both drugs showed high levels in the bottom, body and neck areas of the gallbladder with no difference by sites. The concentration ratios against serum for these drugs were 71.8 and 83.6%, showing good transference into tissue. The mean concentrations of CPZ and PIPC in the cholecystic bile were as high as 448.8 and 381.6 micrograms/ml, the ratio against serum being about 4:1. The serum concentrations of PIPC at the 1st and 7th postoperative days were 116 and 137 micrograms/ml at 60 minutes after starting drip infusion, with no difference between these 2 days. In the bile concentrations of PIPC on both postoperative days showed the peak levels of 2,587 and 1,157 micrograms/ml at 120 minutes after starting drip infusion. The concentrations at the 1st postoperative day were higher. The recovery rate of PIPC from the bile was also higher at the 1st postoperative day. From the results that both drugs showed high levels in the wall of gallbladder and also showed high levels in the bile immediately after the operation, PIPC and CPZ are considered to be effective drugs for biliary tract infections.

Bile↗
PubMed1984

[Clinical studies on multiple lacunar state].

Sixty-five cases of clinically diagnosed multiple lacunar state have been analysed. The clinical course of multiple lacunar state is usually progressive in nature, simulating degenerative diseases, in contrast with the mode of acute onset seen in the other vascular diseases. Average age at the initial visit was 63.8 year-old, and sex ratio showed marked male preponderance, being 12 for males and 1 for females. Gait disturbance and speech disturbance are the most frequent initial symptoms, followed by slow motion, emotional lability and swallowing difficulty. Neurological manifestations are dysarthria, short-stepped and apraxic gait, hyperreflexia, positive Babinski and Chaddock reflexes, minimal spasticity, dementia, positive palmo-mental reflex, emotional lability, fixed face, rigidity, bradykinesia, foot grasping, dysphagia, positive Myerson's sign and tremor, in the order described. The important point is that the rigidity is paratonic and the tremor is action or postural, not the cogwheel rigidity or resting tremor like Parkinson's disease. The appearance of pathological reflexes (Babinski and Chaddock reflexes) are quite important, especially Chaddock reflex, which can frequently become positive despite negative or equivocal Babinski reflex. Hypertension, especially longstanding in nature, is the major contributing factor in this disorder. CT scan showed the presence of one or more lacunes in 52 out of 65 cases (80.0%). The detection of lacune can be influenced by the quality of CT scan, and the high resolution CT scanner is greatly useful for that purpose. It is stressed that the detailed neurological and computed tomographic evaluations will make it possible to reach the clinical diagnosis and appropriate treatment of multiple lacunar state.

Adult↗
PubMed1983

Salivary gland glucagon is a fictitious substance due to tracer-degrading activity resistant to protease inhibitors.

A high level of glucagon immunoreactivity was apparently detected in acid-saline extract from rat submandibular glands, but tracer glucagon added to the assay mixture was mostly damaged in spite of the presence of protease inhibitors commonly used in radioimmunoassay. Gel-filtration of the extract on a Bio-Gel P-10 column revealed strong tracer-degrading activity at the void fraction where the apparent immunoreactivity was eluted. Serial changes in apparent immunoreactivity of the extract fit well on the theoretical curve of an exponential tracer degradation. These findings indicate that the salivary gland glucagon is a fictitious substance due to tracer degradation during radioimmunoassay. Further study revealed that the glucagon molecule was hydrolyzed at the arginyl bonds and split into two fragments during incubation with the acid-saline extract from rat submandibular glands.

Animals↗
PubMed1983

Carbonic anhydrase-III immunohistochemical localization in human skeletal muscle.

Using the indirect immunoperoxidase method, we studied the localization of carbonic anhydrase-III (CA-III) in frozen sections of biopsies of human skeletal muscle which had no definite pathology. CA-III was found to be localized in Type-I muscle fibers when compared with serial sections stained with myosin ATPase and other reactions. Our finding was in accordance with the biochemical data so far reported. It was though that CA-III could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases.

Adenosine Triphosphatases↗