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Biomedical subjects

K Seyer-Hansen

Publications and source records attributed to K Seyer-Hansen.

At least 37 records · Page 2Linked to original sources
PubMed1980

The rate of synthesis and breakdown of glomerular basement membrane material at the transition 'normal to diabetes' and 'diabetes to normal'.

A considerable enlargement of the renal glomeruli is present very early after the acute onset of juvenile diabetes mellitus. In the hypertrophied glomeruli the surface area of the capillary walls, the filtration surface, is increased, and thereby also the amount of basement membrane in the capillary wall. The rate of development of this type of diabetic basement membrane accumulation has been studied in streptozotocin diabetic rats with 4- and 47-day duration of diabetes. During the first 4 days a 40% increase in the total amount of peripheral basement membrane material occurs, whereas the following period up to 47 days shows no further change. The structural changes of the glomerular capillaries seen in conjunction with the basement membrane accumulation comprise an enlargement of the filtration surface and capillary luminal volume. In another experiment, 4-week diabetic animals were transplanted with pancreatic islets. The renal structures were studied quantitatively after 4 weeks of normalization. It was found that the increased amounts of basement membrane material were still present.

Animals↗
PubMed1979

The clinical diagnosis of insulinoma.

Based on the literature and on experience and on experience with 23 cases the clinical diagnosis of insulinomas is reviewed. In clinical praxis the diagnostic steps can usually be simplified to a demonstration of fasting, symptomatic hypoglycaemia. Hence, we have used the 72-hour fast as the single diagnostic test for insulinomas during the last 5 years. Pro-insulin measurements may be a valuable supplementary tool. Other diagnostic tests are probably of little importance.

Adenoma, Islet Cell↗
PubMed1978

Renal hypertrophy in experimental diabetes: a comparison to compensatory hypertrophy.

The kidney growth seen after the induction of experimental diabetes in rats has been compared to the compensatory renal growth after one-sided nephrectomy. After five days the kidney weight had increased from 650 +/- 15 mg in a group of controls to 778 +/- 21 mg in diabetic rats, and 764 +/- 17 mg in unilaterally nephrectomised rats. The increased weight was in both groups reflected in an increased DNA content and increased RNA/DNA and protein/DNA ratios. In a group of rats made diabetic and nephrectomised at the same time, kidney weight increased to 953 +/- 22 mg after five days. When rats were unilaterally nephrectomised after 20 days of untreated diabetes, compensatory growth was much more pronounced than in non-diabetic rats, kidney weight rising from 780 +/- 21 to 1144 +/- 39 mg in five days. Similarly, in rats with established compensatory renal hypertrophy, diabetes induced a very rapid growth of the remaining kidney (1226 +/- 46 mg after five days).

Animals↗
PubMed1978

Growth hormone enhances basement membrane thickening in experimental diabetes. A preliminary report.

Injection of porcine growth hormone (200 microgram) or saline 5 days a week for 16 to 20 weeks in streptozotocin-diabetic rats showed that compared to saline growth hormone produced a 2 1/2-fold larger increase in glomerular capillary basement membrane thickness (2p = 0.027). The possible significance of this effect of an elevated level of growth hormone for diabetic microangiopathy is discussed.

Animals↗
PubMed1977

Renal hypertrophy in experimental diabetes: relation to severity of diabetes.

Streptozotocin diabetic rats have larger kidneys than non-diabetic rats. In the present study the rate of kidney growth during the first seven days of diabetes was correlated with the blood glucose concentration. Over a wide range of blood glucose concentrations (116-340 mg/100 ml) the kidney weight, protein content and protein/DNA ratio were closely correlated with the glucose values.

Animals↗
PubMed1976

Failure of somatostatin to correct manifest diabetic ketoacidosis.

Juvenile diabetic patients were studied 60-72 hours after insulin withdrawal when moderate ketoacidosis had developed. Somatostatin infusion for 4 hours in five patients resulted in almost complete suppression of plasma pancreatic glucagon and growth hormone, a fall in plasma-cyclic-adenosine-monophosphate (A.M.P.) concentrations, and a large fall in plasma-glucose concentration. After infusion plasma concentrations of these substances rose again. Blood-ketone-bodies, plasma-free-fatty-acids (F.F.A.), and plasma glycerol concentrations, however, did not decrease appreciably with somatostatin administration. In three patients 2 to 3 h somatostatin infusions were twice superimposed upon a continuous 9-5 h insulin infusion (1 unit/h). An insulin effect was noticeable within 30 minutes, with pronounced falls in the concentrations of plasma glucose, pancreatic glucagon, F.F.A., and blood-ketone-bodies. There was no significant change in these patterns when somatostatin was administered or withdrawn. These results do not indicate that somatostatin infusion would be useful in the treatment of manifest diabetic ketoacidosis.

Adult↗
PubMed1976

Renal hypertrophy in streptozotocin-diabetic rats.

1. Kidney weight and content of protein, RNA and DNA were measured in rats with streptozotocin diabetes of varying duration. 2. Diabetic rats had larger kidneys than control rats: after 3 days of diabetes the weight increase was 15 per cent and after 42 days of diabetes it was 90 per cent. The protein content rose in parallel to the weight. 3 RNA content was already increased after 36 h of glycosuria, whereas DNA content was unchanged for the first 3 days of diabetes, and increased thereafter. The protein/DNA ratio increased rapidly during the first 3 days but remained constant thereafter. 4. Insulin treatment decreased the renal weight gain by about 67 per cent during the first 8 days of diabetes, but did not prevent the increase in DNA. When insulin was started after 25 days of diabetes there was only a slight regression of kidney growth.

Animals↗
PubMed1975

Elevated serum growth hormone in protein-deprived rats, and decreased liver RNA after hypophysectomy.

Serum growth hormone was significantly higher in rats fed on a non-protein than on a 25% casein diet. In both dietary groups, hypophysectomy provoked a decrease in the RNA/DNA ratio of the liver, and, especially in the protein-deprived rats, a decrease in the RNA synthesis per liver cell, measured as the product of the RNA/DNA ratio, and the ratio of specific RNA activity to specific nucleotide activity, two hours after administration of [3H]orotic acid. In the protein-deprived rats, full reconstitution of these figures was obtained with a combination of growth hormone, hydrocortisone and triiodothyronine, but not quite with growth hormone alone. The possible role of growth hormone in protein deprivation is discussed.

Animals↗