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Biomedical subjects

K Seta

Publications and source records attributed to K Seta.

At least 19 recordsLinked to original sources
PubMed1992

A rat cerebellar protein containing the cdc10/SWI6 motif.

Systematic analysis of soluble proteins in developing rat cerebellum by an automated two-dimensional liquid-chromatography system detected a number of proteins which increased transiently during the initial stage of postnatal development. One of the proteins, V-1, was isolated using a liquid-chromatography system, and its amino acid sequence was determined by analysis of the purified protein. The sequence showed that the V-1 protein consists of 117 amino acids with an acetylated N-terminus, and has 2.5 internal sequence repeats of 33 amino acids. Computer retrieval of the sequence indicated that the repeated sequences have a structural characteristics of the cdc10/SWI6 motif, which is found in a series of proteins, including those involved in cell-cycle control and cell-fate determination in yeast, Drosophila melanogaster and Caenorhabditis elegans. The structure of V-1, coupled with its controlled expression in early postnatal development, implies a potential role for V-1 in cerebellar morphogenesis.

Amino Acid Sequence↗
PubMed1990

Determination of alpha-tocopherol, free cholesterol, esterified cholesterols and triacylglycerols in human lipoproteins by high-performance liquid chromatography.

The determination of alpha-tocopherol, free cholesterol, esterified cholesterols and triacylglycerols in human plasma and in fractions containing individual lipoproteins was achieved by reversed-phase high-performance liquid chromatography (HPLC). The lipoprotein fractions, such as chylomicron, VLDL, LDL, HDL2 and HDL3, were collected by ultracentrifugation of human plasma. The chromatographic separation was accomplished with a column packed with Hitachi Gel 3057, which is a spherical octadecylsilica of particle size 3 microns. The mobile phase was acetonitrile-2-propanol (75:25, v/v), and the eluate was monitored with ultraviolet absorption and fluorescence detectors connected in series. Qualitative analysis of the main chromatographic peaks collected during the HPLC of a plasma sample was done with the use of field-desorption mass spectrometry. The determination analysis of alpha-tocopherol, free cholesterol and esterified cholesterols was effected with a single chromatographic run with n-hexane extracts of plasma or lipoprotein fraction. The separation and determination of these fat-soluble components required as little as 5 microliters of plasma or lipoprotein fraction.

Cholesterol↗
PubMed1989

[Effect of LDL-apheresis on a case of Cerebrotendinous Xanthomatosis].

Cerebrotendinous Xanthomatosis (CTX) is a rare familial disease characterized by tendon-xanthomas, cataracts, progressive cerebellar ataxia, dementia and an elevation of serum cholestanol with normal levels of cholesterol. Although the pathogenesis of CTX is not fully understood, increment of cholestanol is suggested one of the major metabolic derangements of the disease. Recently, the LDL-apheresis has been developed as a new therapeutical equipment in the field of hyperlipidemia and been widely used to reduce the levels of LDL-cholesterol by selective LDL adsorption. From the point of view that cholestanol is involved mainly in LDL-cholesterol (1.019 less than d less than 1.063), we used this LDL-apheresis in the aim of reducing the cholestanol in 58 years old woman with typical sign and symptoms of CTX. The levels of serum cholestanol and cholesterol before the treatment with LDL-apheresis, were 10.7 micrograms/ml and 175 mg/dl respectively. Also the ratio of cholestanol/cholesterol indicated 0.63. By the first procedure of apheresis, the level of cholestanol was markedly decreased to 5.2 micrograms/ml (50%). Several LDL-apheresis treatments were carried out once a month. During 5 months treatments, neurological deterioration was arrested, dementia which included disorientation and recent-memory loss, cleaned a little. Although the xanthomas did not decrease in size, this patients was better oriented to person, place, time and was able to speak rationally, 2nd her cerebellar dysfunction revealed improvement. From our new experiments-we believe that the LDL-apheresis offers the strong hope of preventing the progress on cerebrotendinous xanthomatosis.

Achilles Tendon↗
PubMed1979

Review of five cases of limy bile--particularly on radiological, chemical and crystallographic studies--.

Incidence of "limy bile" is relatively rare and only 120 cases have been recorded in Japan so far. The present report is to add five more cases operated on at our clinic. On chemical analysis by atomic absorption spectrophotometry calcium carbonate was the major constituent of solid portion and ranged from 73.5 to 88.5%. On crystallographic analyses, the infrared spectra by means of KBr-disk method gave patterns of calcium carbonate in all cases, and the X-ray powder diffraction studies disclosed those of aragonite only in cases 2 and 4, and aragonite-calcite mixture in cases 1 and 3. The exact etiology of formation of limy bile in the gallbladder has not yet been understood completely.

Adult↗
PubMed1978

Introduction of a method of valvuloplastic esophagogastrotomy in proximal gastrectomy.

A technique of valvuloplastic esophagogastrostomy is proximal gastrectomy to control postoperative reflux esophagitis is reported. After proximal resection of the stomach, the medial stump is closed in two layers. The mucosal layers of the lateral stump are sewn but leaving the seromuscular layers open. The esophagus is anastomosed to the mucosal stoma at the middle of the gastric stump. The distal esophagus is wrapped by the lateral stump like Nissen's fundoplication to create the artificial fundus. Intragastric esophageal wall facing the fundus acts as a long one-way flap valve to prevent reflux. Ten dogs were prepared with this method and were compared with end-to-end and end-to-side anastomosis prepared in five dogs each. Cinefluoroscopy and esophageal pH demonstrated various degress of reflux in all the dogs with end-to-end and end-to-side anastomosis, and mild reflux in one out of the ten dogs with valvular anastomosis. A sharp rise in pH at the anastomotic site was consistent in the remaining nine valvuloplastic dogs. High pressure zone, 9.9 mmHg on an average at the site of anastomosis was present in valvuloplastic dogs, while the pressure was 0 mmHg in end-to-end and 5.2 mmHg in end-to-side anastomosis dogs. Clinical application of this procedure in ten patients obtained satisfactory results. The technique offers a reliable method of valvuloplastic anastomosis in esophagogastrostomy.

Animals↗
PubMed1976

Histological appearance of small thyroid carcinoma.

Histopathological characteristics of the tumor growth were studied in 59 small carcinomas detected in thyroids at autopsy and in 33 from surgically removed thyroids. Tumor size was less than 5 mm in 56 of the 59 carcinomas (95 per cent) detected in autopsy materials. Histological findings of the small carcinomas were papillary adenocarcinoma in 45 of 59 (76 per cent), and sclerosing carcinoma in 32 (54 per cent). Among these 59 small carcinomas, intrathyroid metastases were found in six (10.2 per cent). In small carcinomas measuring less than 5 mm, carcinomas in females had an average diameter of 2.19 mm and were significantly larger than those found in male, having an average diameter of 1.14 mm (p less than 0.05). In small carcinomas from the surgical specimen, incidence lymph node metastasis was high when associated with numerous intrathyroid metastases and when the distance was great between the edge of the primary tumor and the farthest satellite metastatic focus.

Adenocarcinoma↗
PubMed1976

Thyroid carcinoma.

Pathological and clinical characteristics of thyroid carcinoma are reported. In 379 routine consecutive autopsies in which carcinoma was not suspected, serial sections of thyroid gland were histologically examined. Latent thyroid carcinoma was detected in 15.7% of the glands. Papillary adenocarcinoma comprised 76.3%, follicular adenocarcinoma 22.0% and trabecular carcinoma 1.7% of the cases. Sclerosing carcinoma was seen in 32 cases. The long diameter of 90% of these tumors was less than 5 mm. Whether or not thyroid carcinoma persists as such a small carcinomatous lesion indefinitely was studied along with its clinical significance. Carcinomatous lesions complicating hyperthyroidism were always less than 1 cm. About 70% were less than 5 mm long. Since proliferation of some thyroid carcinomas was inhibited by TSH suppression therapy, growth and proliferation of carcinoma in the presence of excessive thyroid hormone or hyperthyroidism is probably already inhibited.

Adenocarcinoma↗