Search PubMed⌕ Search

Biomedical subjects

K Selkäinaho

Publications and source records attributed to K Selkäinaho.

8 recordsLinked to original sources
PubMed1986

Quantitation of staining for amyloid in histological sections: sources of variation, correlation analysis, and interstain reproducibility.

Five pathologists estimated with point counting the amount of amyloid in heart muscle autopsy samples from 19 patients who suffered from amyloidosis. The serial sections had been stained with 5 different methods. The different variation sources involved in a quantitative study on amyloid and the interstain reproducibility between the results given by these 5 stains were analyzed. The volume fractions of amyloid after different stains were subjected to correlation analysis. In our test system about a third (34%) of the variation was due to the point counting method. Sirius red had the lowest and Congo red the highest variation due to point counting, since Sirius red gave the most intense staining and highest volume fractions for amyloid. The volume fractions were lowest in Congo red. About 40% of the total variation was composed of the field variation. This variation includes the variation due to variation between fields, field selection and subjective intraobserver variation. Sirius red showed the smallest field variation in respect to the total variation. Congo red, on the other hand, had the highest variation. The variation caused by pure interobserver variation is presented by the difference between total variation and the calculated field variation. This was largest with Sirius red and smallest with Congo red. The pure interobserver variation accounted for about 26% of the total variation. The high reproducibility with Sirius stain is most probably due to the high volume fractions of positively stained material after this stain, not to low interobserver variation. Congo red seems to have low interobserver variation. The senior pathologists were more reliable in measuring the amyloid deposits than the junior pathologists as suggested by interstain reproducibility and variation.(ABSTRACT TRUNCATED AT 250 WORDS)

Amyloid↗
PubMed1986

Evaluation of carcinoembryonic antigen and periodic acid-Schiff stains in the diagnosis of serous and mucinous ovarian tumors: morphometrical study.

108 serous and 104 mucinous neoplasms of the ovary, classified according to the criteria of the World Health Organization, were studied with periodic acid-Schiff (PAS) method for mucin and glycogen, and with immunoperoxidase method for carcinoembryonic antigen (CEA). The amount of epithelium, the number of mitoses, and the staining positivities were quantitated morphometrically and the parameters were evaluated using the discriminant analysis. In general, CEA scores were higher in mucinous than in serous tumors and higher in malignant tumors than in their benign or borderline counterparts. High PAS positivities were found both in serous and mucinous borderline lesions. Mitotic activity correlated with the malignancy of the tumors. The best discriminator, however, was the amount of epithelium. The results show that simple morphometry with standard stains is an efficient method in the first-line classification of these tumors. The quantitation of CEA and PAS stains may be helpful in cases in which histological criteria do not allow definite classification.

Adenocarcinoma, Mucinous↗
PubMed1985

Reproducibility of morphometric measurements of amyloid after various staining methods.

Four histologic staining methods used for detecting amyloid (Congo red, viewed in both normal and polarized light, Sirius red, Crystal violet and Thioflavine T) were applied to heart muscle autopsy samples from 19 patients who suffered from amyloidosis. The amount of amyloid present was evaluated with morphometry (point counting) by five pathologists, and the interobserver reproducibility and variation of point counting in these staining methods were analyzed. The Sirius red method showed the least variation and was the most suitable stain for demonstrating amyloid with respect to reproducibility. Thioflavine T showed the greatest variation and was the least suitable stain with respect to reproducibility. The range of variation was considerable in all staining methods. The results show that stains differ in their specificity and sensitivity in staining amyloid, observers differ in their interpretation of staining results and certain stains result in more uniform interpretations than do others.

Amyloid↗
PubMed1985

Reproducibility and variation of morphometric analysis of carcinoembryonic antigen staining in histopathology. The influence of standardized microscopic fields.

Using morphometric methods, five pathologists analyzed the positive staining for carcinoembryonic antigen (CEA) in sections from 17 ovarian tumors, with the intraclass correlation coefficient (ICC) and the mean values of the coefficients of variation (CV) used to assess reproducibility and variation. First, field and point scores for epithelium and mucin were estimated using 25 randomly selected square fields in sections from each of the tumors. The ICC range in the whole sample field was 0.53 to 0.81 (slight to substantial reproducibility) while the mean values of CV were 0.50 to 0.75. Second, the results of using random and standardized individual fields for the measurements were studied in three tumors. In random fields, the ICC was 0.57 to 0.71 (slight to moderate reproducibility) and the mean values of CV were 0.53 to 0.65. The corresponding values in standardized fields were 0.71 to 0.73 (moderate reproducibility) and 0.41 to 0.57, respectively. The results show that the variation is smaller and the degree of reproducibility higher in standardized fields. Considerable variation remains, however, revealing human factors as an important source of variation in practical morphometry.

Carcinoembryonic Antigen↗
PubMed1984

Morphometry of articular cartilage: a stereological method using light microscopy.

A stereological method is introduced for studying the light microscopic morphometry of articular cartilage. New parameters are presented for defining the three-dimensional structure of the articular cartilage. These include volume density of the lacunae and nuclei, numerical density of cells in 3-dimensional space, total number of cells, total volume of the lacunae in cartilage, volume and diameter of the lacuna and nucleus, the nucleus/lacuna volume ratio, and the mean volume of the matrix per cell. Using the lateral tibial condyle of the knee joint of eight rabbits, we investigated tissue preparation, sampling, morphometry, and the conditions necessary for validity and precision of the estimators. The method proved easy to use, and the estimators were precise.

Animals↗
PubMed1984

Methodological variation in systolic time intervals.

Systolic time intervals (STI) were recorded six times in 12 male students. All 72 recordings were measured twice by a physician and once by two nurses. By comparing these measurements an intra- and interobserver (= methodological) variation was obtained. For measured parameters (QS2- interval and LVET) variation was less than 2%. For calculated parameters the variation was larger, but did not reach the level of statistical significance. Methodological variation was smaller than physiologic variation. The internal consistency was almost perfect for measured parameters and only slightly worse for calculated parameters.

Adult↗
PubMed1980

Relationship between reported toothbrushing and dental caries in adults.

The relationship of toothbrushing to dental decay has remained unclear. In the present study an effort was made to throw more light on this problem. Clinical and interview data were obtained from 212 males. Potential confounding risk indicators were controlled by a multivariate confounder summarizing score. In general it was found that the values of caries prevalence indicator were consistently higher for sporadic toothbrushers. It was concluded that the current study provides evidence in favor of a positive association between toothbrushing and low caries prevalence.

Adult↗