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Biomedical subjects

K Schmied

Publications and source records attributed to K Schmied.

4 recordsLinked to original sources
PubMed2006

Analysis of subcellular surface structure, function and dynamics.

Analytics of single biological cells allows quantitative investigation from a structural, functional and dynamical point of view and opens novel possibilities to an unamplified subcellular analysis. In this article, we report on three different experimental methods and their applications to single cellular systems with a subcellular sensitivity down to the single molecule level. First, the subcellular surface structure of living bacteria (Corynebacterium glutamicum) was investigated with atomic force microscopy (AFM) at the resolution of individual surface layer (S-layer) proteins; discrimination of bacterial strains that lack the expression of hexagonally packed surface layer proteins was possible. Second, quantitative measurement of individual recognition events of membrane-bound receptors on living B-cells was achieved in single cell manipulation and probing experiments with optical tweezers (OT) force spectroscopy. And third, intracellular dynamics of translocating photoactivatable GFP in plant protoplasts (Nicotiana tabacum BY-2) was quantitatively monitored by two-photon laser scanning microscopy (2PLSM).

Animals↗
PubMed1987

Analogues and derivatives of tenoxicam. 1. Synthesis and antiinflammatory activity of analogues with different residues on the ring nitrogen and the amide nitrogen.

The synthesis of tenoxicam, 4-hydroxy-2-methyl-N-2-pyridyl-2H-thieno[2,3-e]-1,2-thiazine-3-carboxami de 1,1-dioxide (1e), and of the analogues with various residues on the ring nitrogen and the amide nitrogen is described. This new class of "oxicams" has pronounced antiinflammatory and analgesic properties. The very specific structure-activity relationship of isomeric and isosteric groups at the amide nitrogen has been evaluated. The substituent in position 2 also has a great influence on the pharmacological properties. Tenoxicam is presently undergoing clinical trials.

Animals↗
PubMed1983

[Isolation and forced injection in the opinion of affected patients and patient care personnel. An accompanied quarter year sample].

All cases of seclusion and emergency sedation at a regional psychiatric university hospital were studied for a period of 3 months. The patients involved, and their nursing staff, were questioned on the day following the above mentioned incidents and the patients again after their remission from the exceptional state that had caused them. Every sixth patient underwent, at least once, either seclusion or emergency sedation, or both. These occurred most frequently during the first week of hospitalization, and in patients with a predominantly negative attitude. Women showed violent behaviour more often than men and also admitted to it more frequently. Seclusion was generally better accepted than emergency sedation. In less than half the cases the patients clearly rejected the past coercive measure; cases of total acceptance, however, were even less frequent, though acceptance tended to increase with remission. The authors dealt with the legal status of coercive measures. They also stressed the importance of discussing them afterwards both with patients and nursing staff in order to preserve the self-respect of all concerned.

Adult↗