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Biomedical subjects

K Sano

Publications and source records attributed to K Sano.

At least 361 records · Page 20Linked to original sources
PubMed1994

Differential expressions of surfactant protein SP-A, SP-B, and SP-C mRNAs in rats with streptozotocin-induced diabetes demonstrated by in situ hybridization.

We have previously demonstrated by in situ hybridization and Northern blot analysis that alveolar type II cells and nonciliated bronchiolar epithelial (Clara) cells in lungs of rats with diabetes have decreased surfactant protein A (SP-A) but increased mRNA. In the present study, we have examined the mRNA expression and localization of two hydrophobic surfactant proteins, SP-B and SP-C, and have compared them with SP-A mRNA levels and cellular localization in streptozotocin-induced diabetic lungs. Localization and level of expression were analyzed by in situ hybridization using type-specific, surfactant cDNA probes. Diabetes was induced in adult rats by an intraperitoneal injection of 60 mg/kg streptozotocin. Ten weeks after injection, higher numbers of silver grains representing SP-A and SP-B mRNAs were observed in alveolar type II cells of diabetic lungs, compared with control lungs. Moreover, the number of silver grains representing SP-C mRNA decreased in alveolar type II cells from diabetic lungs compared with controls, and no silver grains for SP-C mRNA were present in bronchiolar epithelial cells from either control or diabetic groups. In contrast, in bronchiolar epithelial cells of diabetic lungs, the relative abundance of silver grains for SP-A mRNA increased approximately 2-fold above controls, while SP-B mRNA decreased slightly. Taken together, there is differential expression in the level of SP-A, SP-B, and SP-C mRNAs in both alveolar and bronchiolar epithelial cells from diabetic lungs when compared with control lungs.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1994

Effects of surfactants and protease inhibitors on nasal absorption of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in rats.

The effects of various surfactants and protease inhibitors on the nasal absorption of recombinant human granulocyte colony-stimulating factor (rhG-CSF) were examined in rats. No effects of bile salts and acids such as sodium glycocholate or taurocholic acid, amphoteric surfactants such as lauryldimethyl betaine, or anionic surfactants such as sodium lauryl sulfate on the absorption were found at a concentration of 1%. But non-ionic surfactants with hydrophile/lipophile balance (HLB) of 13 to 18 increased the total leukocyte numbers maximally by about 250% as a relative increase ratio to the control without surfactants. The increase in the plasma rhG-CSF concentration was obviously observed only in the presence of non-ionic surfactants, and in particular, the effects of Laureth-9 on the increase in total leukocyte numbers and plasma rhG-CSF concentration were maximal. In the presence of various kinds of protease inhibitors, the increasing effect of rhG-CSF on the total leukocyte numbers was not changed. Consequently, it is considered that the permeation of rhG-CSF through the nasal epithelium can be improved by non-ionic surfactants, but the effect of a protease inhibitor is smaller than that of the surfactant.

Absorption↗
PubMed1994

Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin.

The entire coding sequences for five possible human cadherins, named cadherin-4, -8, -11, -12 and -13, were determined. The deduced amino acid sequences of cadherin-4 and cadherin-13 showed high homology with those of chicken R-cadherin or chicken T-cadherin, suggesting that cadherin-4 and cadherin-13 are mammalian homologues of the chicken R-cadherin or T-cadherin. Comparison of the extracellular domain of these proteins with those of other cadherins and cadherin-related proteins clarifies characteristic structural features of this domain. The domain is subdivided into five subdomains, each of which contains a cadherin-specific motif characterized by well-conserved amino acid residues and short amino acid sequences. Moreover, each subdomain has unique features of its own. The comparison also provides additional evidence for two structurally different types of cadherins: the first type includes B-, E-, EP-, M, N-, P- and R-cadherins and cadherin-4; the second type includes cadherin-5 through cadherin-12. Cadherin-13 lacks the sequence corresponding to the cytoplasmic domain of typical cadherins, but the extracellular domain shares most of the features common to the extracellular domain of cadherins, especially those of the first type of cadherins, suggesting that cadherin-13 is a special type of cadherin. These results, and those of other recent cloning studies, indicate that many cadherins with different properties are expressed in various tissues of different organisms.

Amino Acid Sequence↗
PubMed1994

[Urological problems in Prader-Willi syndrome].

Prader-Willi syndrome is rather a rare disease. However, as it includes 4 features (hypogonadism, hypomentia, hypotonia, and obesity), urologist may see the patients with this syndrome for their gonadal problem. We studied all the 27 cases in our hospital of which data were precisely collected. Among males, 67% of patients had presented themselves first to the department of pediatric internal medicine. One third of the patients were not diagnosed as the syndrome and referred to our clinic because of urological abnormalities. Chromosomal abnormality was seen in 40.9%. We found cryptorchism in all the cases and micropenis in 56%. In females, delayed menarche over 15-year-old was seen in 75%. From endocrinological studies, 75% male showed low reaction against HCG stimulation. Low gonadotropin responses to LH-RH were seen in 80% of all patients. We performed orohiopexy for cryptorchism, but testicular development was poor. And we do not actively utilize hormonal therapies for these patients.

Adolescent↗
PubMed1994

Cytomegalovirus infection in living related liver transplantation: rapid diagnosis by human monoclonal antibody staining of blood leucocytes.

Symptomatic cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following orthotopic liver transplantation. Early detection and prompt treatment with appropriate medicine are crucial for successful outcome. As an early detection method, we used the CMV antigenemia test which is based on immunocytochemical detection of CMV immediate early antigens in blood leucocytes. CMV immediate early antigens were detected in blood leucocytes with direct immunoperoxidase technique using a horseradish peroxidase (HRP)-conjugated F(ab')2 fragment of human monoclonal antibody (humab C7), designated HRP-C7. Of the 37 living related liver transplantations we performed on pediatric patients between June 1990 and August 1992, we experienced four cases (10.8%) of symptomatic CMV infection: three CMV hepatitis and one CMV peritonitis. They were all treated successfully with a combination of ganciclovir and immunoglobulins. In ABO-incompatible cases ganciclovir was administered prophylactically. Early detection using this method is though to have led to these successful outcomes. It is concluded that HRP-C7 staining of blood leucocytes is useful for the rapid diagnosis of CMV infection.

ABO Blood-Group System↗
PubMed1994

[Relationship between power spectra of breath sounds and inspiratory flows at different frequencies].

Regional ventilation and changes in airway caliber have been studied by analysis of breath sounds. The aim of this study was to find which frequency band is most suitable for such purposes. The subjects were 19 healthy men. Breath sounds at 6 sites on the chest wall, airflow rate at the mouth, and ECG were digitized and recorded for 30 seconds. Power spectra of breath sounds at various frequency bands (10-50 Hz, 50-100 Hz, 100-150 Hz, ...., 950-1000 Hz) were calculated with a fast-Fourier transform (FFT) for every block of 512 points (102.4 msec), and mean airflow rates for the blocks were calculated. Data recorded during inspiration when airflow ranged between 0.5 L/s and 3 L/s were analyzed. For frequency bands from 150 Hz to 850 Hz the logarithms of the power spectra were linearly correlated with the logarithms of the airflow rates, and the correlation coefficients exceeded 0.8, but for lower frequency bands the correlations were poor. When the data contaminated by heart sounds and those recorded late in inspiration were excluded the correlations at frequency bands below 150 Hz improved. The slope of log (power)/log (flow) was about 4 for the bands from 100 to 300 Hz, but it became steeper (4 to 6) for higher frequency bands. This means that the power spectra were proportional to the fourth power of the airflow rate for bands below 300 Hz, but the relation was from the fourth to the sixth power for higher frequency bands.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1994

Mucinous cystadenocarcinoma of the pancreas with mucinous biliary obstruction due to uncommon complication of fistulas to the common bile duct and main pancreatic duct.

We report a case of biliary obstruction by a large amount of mucinous material produced by a cystadenocarcinoma of the pancreas invading the common bile duct. This diagnosis was made preoperatively by various imaging methods. Endoscopic retrograde pancreatography (ERP) revealed a mucus-containing cyst with a communication to the main pancreatic and common bile ducts due to a fistulization. Mucinous biliary obstruction was observed. This case indicates that mucinous biliary obstructions should be considered in patients with a mucinous cystic neoplasm of the pancreatic head in the presence of jaundice and that mucinous cystadenocarcinoma may develop into mucinous carcinoma, especially in cases with fistula formations. ERP is useful for detecting the presence of a fistula to the common bile duct or other neighboring organs in such cases. Surgical treatment is recommended because the prognosis of mucinous cystadenocarcinoma is better than that of the usual pancreatic cancer, even though the tumor shows a communication with neighboring organs.

Bile Ducts↗
PubMed1994

[Practical CT classification for thalamic hemorrhage: relationship between localization of hematoma and prognosis].

It is not easy to predict functional outcome in patients with acute-stage thalamic hemorrhage. We analysed 100 cases of hypertensive thalamic hemorrhage less than 4 cm in diameter, and devised a practical CT classification for predicting the patients' prognoses. On an axial CT scan at the level of the pineal body, four lines were drawn as follows: line (a) between the lateral edge of the anterior horn and the midpoint of the third ventricle; line (b) vertical line to the sagittal line from the midpoint of the third ventricle; line (c) between the lateral edge of the trigone and the midpoint of the third ventricle; line (d) between the lateral edge of the anterior horn and the lateral edge of the trigone. The location of hematoma was divided into three types according to lateral extension as follows: type A (anterior type), center of hematoma located between line (a) and line (b); type P (posterior type), center of hematoma located between line (b) and line (c), and external margin of hematoma localized medial to line (d); type PL (postero-lateral type), center of hematoma located between line (b) and line (c), and showing lateral extension beyond line (d). Then, the correlation between hematoma location and severity of motor paresis at onset and its prognosis was investigated. Severe hemiparesis (MMT: 0-2) was observed in 15.3% of patients with type A, 21.8% with type P, and 59.3% with type PL hematoma in the acute stage.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1994

A case of infant with bilateral multicystic kidney and ureteropelvic junction obstruction; surgical treatment after percutaneous nephrostomy.

We report a case of bilateral multicystic kidney and left ureteropelvic junction (UPJ) obstruction in a female infant. She was diagnosed to have severe cystic disease from 20 weeks of gestation. After birth with cesarean section, she was transferred to the neonatal intensive care unit (NICU) of our hospital. One day after birth, she was referred to our department for progression of azotemia. We placed a nephrostomy catheter into the largest renal cyst, but it did not work. One week later, we placed another nephrostomy catheter into another cystic lesion inside of the first one. It worked well for nine months and azotemia was improved. At the age of nine months her upper urinary tract was reconstructed by pyeloplasty. We observed peristalsis of ureter and first urination from bladder.

Female↗
PubMed1993

Mapping of replication initiation site in Mycoplasma capricolum genome by two-dimensional gel-electrophoretic analysis.

The homolog of the dnaA gene, which has been reported to be present in the vicinity of the initiation site of replication in the genome of Mycoplasma capricolum (M.Miyata, L.Wang, and T.Fukumura, J. Bacteriol. 175: 655-660, 1993) was mapped precisely. A 9540-bp region containing the dnaA gene was cloned and the entire region was sequenced with the exception of a previously reported region of 2517 bp (Fujita, M.Q., Yoshikawa, H. and Ogasawara, N. Gene 93: 73-78, 1992). The organization of the 9540-bp region was compared with that of corresponding regions in other bacteria. The arrangement and directions of rnpA, rpmH, dnaA, dnaN were conserved, but no other open reading frames were found that were homologous to those that are commonly found around dnaA genes in other bacteria. The directions of movement of the replication fork around the dnaA gene were analyzed by neutral/alkaline two-dimensional gel electrophoresis. The forks developed in a 1569-bp region that consisted of the dnaA structural gene and its downstream non-coding region, and then they proceeded bidirectionally.

Amino Acid Sequence↗
PubMed1993

Expression of smg p25A/rab 3A guanine nucleotide dissociation inhibitor (GDI) in neurons and glial cells from rat brain.

smg p25A/rab 3A is a member of the rab family of small guanosine 5'-trisphosphate (GTP)-binding proteins, which are postulated to act as specific regulators of membrane trafficking in exocytosis and endocytosis. In neural tissues, smg p25A/rab 3A is expressed only in neurons but not in glia. In this study, expression of a regulatory protein for smg p25A/rab 3A, guanine nucleotide dissociation inhibitor (smg p25A/rab 3A GDI), in neurons and glia isolated from rat brain was examined. The smg p25A/rab 3A GDI mRNa was detected as early as on gestational day 14 in the fetal brain, and its level reached a maximum 5 days after birth. By immunoblot analysis, smg p25A/rab 3A GDI was detected in the homogenate of rat hippocampus as well as in that of astrocytes in primary culture. By immunocytochemical analysis, the smg p25A/rab 3A GDI immunoreactivity was detected in hippocampal neurons and oligodendrocytes. The immunoreactivity of astrocytes was much lower than that of neurons and oligodendrocytes. These results indicate that smg p25A/rab 3A GDI may play a fundamental role in membrane trafficking both neurons and glia by interacting with multiple rab family proteins.

Animals↗