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Biomedical subjects

K Sakurada

Publications and source records attributed to K Sakurada.

169 records · Page 10Linked to original sources
PubMedDate not supplied

Effect of an inhibitory factor for protein kinase C on cell growth of leukemic cells.

A factor which inhibits protein kinase C has been detected in normal human lymphocytes and leukemia cells obtained from some untreated patients with lymphoblastic leukemia. The partially purified inhibitory factor obtained from normal human lymphocytes depressed the growth of K562 cells and HL-60 cells, while it also suppressed DNA synthesis of these cells in a dose-dependent manner. The inhibitory factor was not able to suppress the DNA synthesis, however when it was added to the medium 12 hours after the culture. The inhibitory factor also reduced the phosphorylation of endogenous proteins, mainly Mr 80,000, 40,000 and 38,000. These results suggest that the inhibitory factor is closely related to the cell proliferation of various cells through the suppression of protein kinase C activity.

Carrier Proteins↗
PubMedDate not supplied

Structure, expression and mutational analysis of the hBRAG gene on 10q in the frequently deleted region in human endometrial cancer.

We previously reported that chromosome arm 10q is one of the target regions of allelic loss in human endometrial cancer. To identify the gene in this region responsible for endometrial cancer, we further characterized this region and localized the hBRAG gene. The function of hBRAG has not yet been fully studied, and there is the possibility that this gene works as a tumor suppressor. This gene consist of 7 exons and 6 introns encoding 503 amino acids; all the introns start with GT and end with AG in agreement with the GT-AG rule. Expression of this gene was studied by Northern hybridization and suppressed expression was observed in one (SK-UT-1B) of the six endometrial cancer cell lines. Mutation analysis in 38 primary EC tissues and six EC cell lines disclosed no genetic alterations. The genomic structure of hBRAG elucidated in this study should contribute to the future analysis of the hBRAG gene.

Carcinoma, Endometrioid↗
PubMedDate not supplied

Cryptic dysfunction of cellular immunity in asymptomatic human immunodeficiency virus (HIV) carriers and its actualization by an environmental immunosuppressive factor.

Peripheral blood lymphocytes from 4 asymptomatic HIV-1 carriers with normally retained EBV-specific cytotoxic T-cell activity were exposed to EBV and incubated with 0.2 ng/ml 4-deoxyphorbol ester, an immunosuppressive substance derived from an African plant Euphorbia tirucalli. The regression of EBV-induced B-cell transformation by EBV-specific cytotoxic T-cells was significantly impaired in the presence of a small amount of 4-deoxyphorbol ester, but not so in 5 HIV-1-seronegative healthy counterparts. When the EBV-specific cytotoxic T-cells from the asymptomatic carriers were exposed to 0.2ng/ml 4-deoxyphorbol ester and incubated with 51Cr-labeled autologous EBV-transformed B lymphocytes, the released radioactivity was significantly smaller than that of the healthy counterparts. The results suggest that the cellular immunity of the asymptomatic HIV-1 carriers is cryptically impaired, and the cryptic immunological dysfunction is actualized by exposure to a small amount of the immunosuppressive substance, a dose which does not affect the immunity of uninfected healthy individuals.

Adolescent↗