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Biomedical subjects

K Sakakida

Publications and source records attributed to K Sakakida.

At least 19 recordsLinked to original sources
PubMed1993

Efficacy and safety of a quick drying rubbing type povidone-iodine alcoholic disinfectant solution.

The efficacy and safety of a quick drying rubbing type disinfectant (HAD Hand Wash) composed of a povidone-iodine (PVP-I) and ethanol were evaluated in the disinfection of transient skin flora on fingers of 30 medical staff of our department. Three millilitres of HAD solution was rubbed onto the hands for three minutes several times a day over a four week period. Bacteriological effects were evaluated twice, on the first day and after four weeks, by the palm stamp method. The rate of colonies sterilized (bacterial reduction rate) after the use of HAD Hand Wash was 86.9 +/- 21.2% (mean +/- SD) on the first measurement, and 91.5% +/- 12.8% at four weeks. Staphylococcus epidermidis was isolated most frequently and this was eradicated in 83.3% of subjects, the bacterial reduction rate 99.7%. Staphylococcus aureus was originally isolated in 14 subjects and was eradicated in 13; the bacterial colony reduction rate was 99.9% showing high antiseptic efficacy. Although the bacterial reduction rate for Bacillus sp. was a rather low, 75.8-100%, this result was not considered problematic from the clinical standpoint. There were no side effects like chapping in spite of an average 104 applications of HAD Hand Wash during the 4-week test period. From these results, HAD Hand Wash was considered highly effective and safe.

Antisepsis↗
PubMed1990

Experiments on antigenicity and osteogenicity in allotransplanted cancellous bone.

This experimental study was undertaken in mice to investigate the procedures for storage of allogenic cancellous bone. Cancellous grafts were were stored at -80 degrees C, -196 degrees C or freeze dried. Grafts were implanted into a defect in the recipient's femur and after one week the cellular kinetic activity was analysed by autoradiography. For immunological study, the grafts were implanted into the recipient's muscle, and 2 weeks later the sensitising properties were examined by killer T-cell toxicity and the complement dependent cytotoxicity test. The capacity for osteogenesis of grafts frozen at -196 degrees C was similar to or higher than those freeze dried, but it was low when storage was at -80 degrees C. Immunogenicity was not affected by these three methods of storage and was very similar to that of fresh autografts. Antigenicity of allografts can be decreased by the freezing procedure. We suggest that the greater osteogenic potency after freezing at 196 degrees C is related to the lesser degree of degeneration of the bone matrix.

Animals↗
PubMed1990

Experimental studies on half-joint transplantation. Antigenicity of fresh allografted articular cartilage and changes in the articular cartilage.

This study was undertaken to examine the fate of articular cartilage in isogenic half-joint grafts implanted after storage by two-stage freezing. The antigenicity was evaluated quantitatively with regard to humoral and cellular immunological responses: none was seen in allogenic cartilage grafts. In the half-joint transplantations, the antigenicity of the cartilage need not be considered; only the antigenicity of cortical and cancellous bone must be reduced. Histologically, half-joint grafts stored at -196 degrees C were superior to fresh allografts. Two-stage freezing, therefore, appears to be useful for the storage of articular cartilage.

Animals↗
PubMed1990

[A preliminary study on the morphological changes of the microvasculature following the peripheral nerve autografting].

The microvasculature of peripheral nerve following autografting was studied by scanning electron microscopy. The newly-formed capillaries was also examined by transmission electron microscopy. Capillaries of a fascicle were divided into an extrafascicular vascular plexus and an intrafascicular capillary bed; the former capillaries formed networks, while the latter capillaries ran longitudinally and formed slightly spiral curve. Normal intrafascicular capillaries had tight junctions, a continuous basement membrane and pericytes. On the suture line, capillaries of the graft and those of the host were connected by newly developed sinusoidal capillaries, which were formed at the extrafacicular region earlier than at the intrafascicular region. Newly developed sinusoidal capillaries were transformed into glomerulus-like capillaries and formed fine networks. These networks exhibited a normal capillary architecture after reconstruction. The endothelium of newly-formed capillaries showed a discontinuous basement membrane at the early stage of regeneration. An intrafascicular capillary bed was present in the middle part of the graft.

Animals↗
PubMed1989

The recovery of growth cartilage after removal of bone bridge between epiphysis and metaphysis.

The in vitro proliferation of chondrocytes from the tibial growth plate of rabbits has been examined following bone bridge formation and its resection in vivo. In vitro 3H-thymidine incorporation by growth cartilage with bone bridges was 53.5% of the contralateral normal growth cartilage, whereas it was 95.7%, following removal of the bone bridges performed five days before sacrifice. Relative 3H-thymidine incorporation into the growth cartilage decreased with increasing discrepancy of tibial length caused by persisting bone bridges. These experiments demonstrated that growth cartilage can recover its proliferative potential following removal of a mechanical growth inhibition caused by the formation of a bone bridge.

Animals↗
PubMed1989

Concentration of interleukin-1 beta in serum and synovial fluid in patients with rheumatoid arthritis and those with osteoarthritis.

The concentration of interleukin-1 beta (IL-1 beta) in serum and synovial fluid of the knee of patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA) was examined using a recently developed radioimmunoassay method. The serum concentration of IL-1 beta was below the detection limit of 0.25 ng/ml in all RA and OA patients. The IL-1 beta concentration in the synovial fluid was higher in RA patients than in OA patients. In RA patients, a significant correlation was demonstrated between the synovial fluid IL-1 beta concentration and the erythrocyte sedimentation rate, C-reactive protein level, synovial fluid white blood cell count and Lansbury index. The synovial fluid IL-1 beta concentration decreased with the reduction of the rheumatoid activity by the medication, but tended to increase or remain high when inflammatory activity of RA could not be adequately controlled. The IL-1 beta concentration, easily determined, shows promise as a new quantitative indicator of rheumatoid activity.

Aged↗
PubMed1989

Immunohistochemical localization of interleukin 1 in human growth cartilage.

Although it has been reported that interleukin 1 (IL-1) stimulate chondrocytes to produce collagenase and proteoglycanase in vitro, IL-1 producing cells and the function of IL-1 have not been demonstrated in osteocartilaginous tissue in vivo. Immunohistochemical studies of human cartilaginous epiphysis and growth cartilage demonstrated that IL-1 was detected in: (1) chondrocytes surrounding cartilage canal, (2) hypertrophic chondrocytes in cartilaginous epiphysis, (3) chondrocytes at the hypertrophic and calcified zones in the growth cartilage of actively growing bone. In contrast, few hypertrophic chondrocytes showed positive reactions to IL-1 in growth plates nearing physiologic closure. Furthermore, IL-1 was detected in chondrocytes cultured from human growth cartilage. These results show that IL-1 is produced by matured chondrocytes of human growth cartilage in vivo. Chondrocyte-derived IL-1 might play a key role in the hypertrophy of chondrocytes, in the vascularization of cartilage and in the formation of bone.

Adolescent↗
PubMed1986

[The relationship between cell kinetics and histological features of giant cell tumor of the bone].

This study was undertaken to clarify the relationship between the proliferative activity and histological findings of the giant cell tumor (GCT) of bone by means of an epi-illumination cytofluorometer (NIKON SPM-RF1-D). Fresh tissues of GCT were surgically obtained from two cases. In both cases, small pieces of tumor tissues were obtained from several different regions based on the macroscopic characteristics of the cut surface, and processed for single cell preparation using enzymatic method. These isolated cells were smeared and stained with acridine orange, and then analyzed cytofluorometrically to determine simultaneously DNA and RNA contents of the individual cells. The results showed that the proliferative activity of tumor cells was much higher in the regions composed of both many histiocytic stromal cells having polygonal or ovoid shape and many multinucleated giant cells, than either in the regions composed of fibrocytic stromal cells accompanying abundant collagen fibers or in the regions composed of foamy cells.

Adult↗
PubMed1985

Rotational alignment of the lower limb in osteoarthritis of the knee.

Rotational alignement of the lower limbs in 43 patients with osteoarthritis of the knee was measured by computerized tomography. Eighty-four limbs were classified in three subsets, patello-femoral osteoarthritis (subset P), medial femorotibial osteoarthritis (subset M) and lateral femorotibial osteoarthritis (subset L). The femorotibial angle of subset M was 5 degrees greater than that of subset P on the average. Femoral torsion of subset P was significantly greater than that of subset M. Femoral torsion in osteoarthritis of the knee was correlated with external tibial torsion and external torsion of the leg, which included both external rotation at the knee and external tibial torsion. External torsion of the leg in subset M was less than normal and rotational alignment of subset P was also outside the normal range. Our findings show that the frontal and rotational alignment of the lower limb are aetiological factors in osteoarthritis of the knee.

Adult↗
PubMed1985

[DNA-RNA cytofluorometric analysis of a case of osteosarcoma in relation to histological characteristics].

We applied DNA-RNA cytofluorometry with AO stain to cell kinetic analysis of osteosarcoma in a 12-year-old girl in relation to its histological characteristics. Histological findings obtained for 9 macroscopically different lesions were grouped into 4 main structural characteristics, but their cytofluorometric results were classified into 2 main patterns of DNA-RNA distribution. One showed remarkable polyploidization with many DNA synthetic cells in the invasive lesions, which were composed of pleomorphic cells forming osteoid or occasionally cartilaginous matrix. The other showed marked accumulation of tetraploid cells almost without DNA synthetic cells, being composed of relatively uniform fibroblastic or stellate-like cells in the cartilaginous matrix. These results indicated a close relationship between cell proliferative activity and the tissue environment.

Bone Neoplasms↗
PubMed1985

The transplantation of an autogeneic osteochondral fragment for osteochondritis dissecans of the knee.

Osteochondritis dissecans in two adults with a large osteochondral defect on the weight-bearing surface was treated by transplantation of an autogeneic osteochondral fragment. The graft was transplanted from the normal portion of the medial femoral condyle, which in extension was in contact with neither patella nor meniscus. The donor site was repaired with an iliac bone fragment. After curettage of the crater, the osteochondral fragment was transfixed with AO mini-cancellous screw(s). Six months after the operation, the grafted cartilage of one patient looked the same as normal cartilage when macroscopically observed and showed no sign of histologic degeneration. At the follow-up examination, the patients were asymptomatic. One patient had a full range of motion two years and three months later; the other three years later. Roentgenographic examinations revealed slight irregularities at the grafted site. There was no significant change in the patellofemoral joint except the concavity of the donor site.

Adult↗
PubMed1985

An investigation of the digital nerves of the thumb.

With the use of a stereoscopic microscope, observations were made on the course of the proper digital nerve of the thumb on ten upper limbs in adult cadavers. By observation from the dorsal side after removal of the bone, it was possible to clearly see the ramification of the digital nerve from the subcutaneous tissue to the dermis. Ramification from the palm to the level of the interphalangeal joint was segmental. The digital nerve of the thumb trifurcates from the proximal site of the distal palmar thumb crease into the main, medial, and lateral branches. The main branch is thick and extends from the center of the pulp toward the fingertip. Ramification at the pulp of the thumb is of a radiating rather than segmental type, and its control can be considered as spotty or intermittent. Distribution of pacinian corpuscles is dense in the subcutaneous tissue of the distal palmar thumb crease. These observations are of clinical importance not only for avoidance of injury to the digital nerve in surgical procedures such as the incision and drainage for felon and the trigger thumb release but also for precise repair of the digital nerve in the reconstructive surgery of the thumb.

Adult↗
PubMed1985

[DNA cytofluorometric analysis of nerve sheath tumors].

The present study was undertaken to clarify the ploidy patterns of peripheral nerve sheath tumors by determining nuclear DNA content of the individual tumor cells using PI-DNA cytofluorometry (NIKON SPM-RF1-D), and to investigate the relation between the ploidy patterns and histopathological findings. Most of the solitary neurilemmomas and neurofibromas studied were found to be associated with euploid-polyploidization, almost without DNA synthetic cells. It was also shown that the number of polyploid cells increased in accordance with an increase in the frequency of cells having large, atypical nuclei in histological picture, regardless of the natures of cellular arrangements. Malignant schwannomas, however, were composed of many polyploid and aneuploid cells with an increase of DNA synthetic cells, indicating their active cell proliferation. Thus, the ploidy patterns of nerve sheath tumors were remarkably different between benign and malignant ones. Furthermore, a case of multiple schwannoma suspected to be a variant of von Recklinghausen's disease, showed euploid-polyploidization with many DNA synthetic cells, indicating a ploidy pattern intermediate between benign and malignant tumors. This tumor thus appears to be a premalignant tumor or in the early stages of malignancy.

Adolescent↗