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Biomedical subjects

K Sahlin

Publications and source records attributed to K Sahlin.

113 records · Page 7Linked to original sources
PubMed1975

Creatine kinase equilibrium and lactate content compared with muscle pH in tissue samples obtained after isometric exercise.

Muscle biopsies taken from the musculus quadriceps femoris of man were analysed for pH, ATP, ADP, AMP, creatine phosphate, creatine, lactate and pyruvate. Biopsies were taken at rest, after circulatory occlusion and after isometric contraction. Muscle pH decreased from 7.09 at rest to 6.56 after isometric exercise to fatigue. Decrease in muscle pH was linearly related to accumulation of lactate plus pyruvate. An increase of 22mumol of lactate plus pyruvate per g of muscle resulted in a fall of 0.5pH unit. The apparent equilibrium constant of the creatine kinase reaction (apparent K(CK)) increased after isometric contraction and a linear relationship between log(apparent K(CK)) and muscle pH was obtained. The low content of creatine phosphate in muscle after contraction as analysed from needle-biopsy samples is believed to be a consequence of an altered equilibrium state of the creatine kinase reaction. This in turn is attributed mainly to a change in intracellular pH.

Adenosine Diphosphate↗
PubMed1975

Adenine nucleotide content of human liver. Normal values and fructose-induced depletion.

A method is described for the determination of metabolites in liver biopsy samples. Normal values of lactate, pyruvate, and adenine nucleotide content of human liver are presented. Infusion of D-fructose, 5.9 mmol9kg-minus l.h-minus l, for 70 min resulted in a 22.5 per cent decrease of the adenine nucleotide pool (mainly by ATP depletion), increased content of fructose 1-phosphate, lactate, and pyruvate. The extent of adenine nucleotide depletion varied greatly between subjects and appeared related to the degree of increase in plasma urate. Infusion of glucose at the same rate did not affect the hepatic content of adenine nucleotides.

Adenine Nucleotides↗