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Biomedical subjects

K Sachse

Publications and source records attributed to K Sachse.

46 records · Page 3Linked to original sources
PubMed1993

[Toxin-producing pasteurellas in a cattle herd].

Besides other agents, indole-negative Pasteurellae, producing dermonecrotic Pasteurella toxin, were isolated from the noses of calves in a herd with enzootic bronchopneumonia. In some blood sera, antitoxin was detected. According to their biochemical activities, isolated strains were classified as P. multocida ssp. septica (ornithine-negative), P. avium (biovar 2), and P. canis (biovar 2). However, in DNA-DNA hybridisation tests there was much coincidence with P. multocida. In experimental calves, pneumonic lesions were produced with one of the isolates as well as with the dermonecrotic toxin. Therefore, indole negative toxinogenic Pasteurellae are considered pneumonia causing agents. They should be taken into account in bacteriological diagnostic and for production of herd specific bacterins.

Animals↗
PubMed1992

Comparison of Mycoplasma bovis strains based on SDS-PAGE and immunoblot protein patterns.

Whole-cell protein patterns generated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were compared for 34 isolates of Mycoplasma bovis. A high degree of similarity between most of the strains was established with strain-to-strain differences mainly confined to quantitative variations of certain protein bands, particularly in the molecular weight regions of 64-68, 55 and 26 kD. Two of the isolates provided more deviating patterns. Hydrophobic membrane protein fractions of the strains as prepared by Triton X-114 phase partitioning were compared by SDS-PAGE, which confirmed some of the characteristic strain features found with whole-cell proteins. The immunoblot analysis revealed that up to 20 of the 50-55 discrete protein bands detected in SDS-PAGE patterns were recognized to be antigenic by rabbit and bovine hyperimmune sera. It is concluded that the same set of major antigens is present in all strains investigated, but amounts of individual constituents may be differing.

Animals↗
PubMed1992

Preparation and characterization of monoclonal antibodies against Mycoplasma bovis.

Monoclonal antibodies (mabs) against Mycoplasma (M.) bovis were prepared for use in diagnosis of bovine mastitis. From the original 32 hybridomas actively secreting mabs against M. bovis, 6 stable lines were cloned. Two of them, Mb 5D8 and Mb 4F6, recognized M. bovis antigens of estimated molecular weights of 33 and 26 kDa, respectively. They showed no cross-reaction to other bovine mycoplasmas, thus rendering them useful for specific detection of this pathogen. All mabs investigated cross-reacted with M. agalactiae which is known to be closely related to M. bovis, but does not occur in cattle. Two other mabs, Mb 5D4 and Mb 1F6, exhibited further cross-reactions to a number of bovine mycoplasma species. Finally, mabs Mb 5D5 and Mb 2G5 reacted with all mycoplasmas tested. The possibility that they recognized constituents of the broth culture medium is discussed.

Animals↗
PubMed1990

Association of cocaine use with sperm concentration, motility, and morphology.

Use of cocaine within 2 years of their first semen analysis has been found to be twice as common among men with sperm counts less than 20 X 10(6) mL (odds ratio [OR] = 2.1, 95% confidence interval [CI] 1.0, 4.6). Duration of cocaine use for five or more years was more common in men with low sperm motility (OR = 2.0, 95% CI 1.0, 4.1) and in those with low concentrations and a large proportion of abnormal forms. Other major risk factors for these three indicators of male subfertility also have been identified, but the cocaine risk factors remained after adjustment for them. This association, together with the high prevalence of cocaine use in the general male population, suggests cocaine may now be related to male subfertility and that history of use should be ascertained during diagnostic interviews.

Adult↗
PubMed1990

A group of indole-negative bovine strains with high deoxyribonucleic acid homology to Pasteurella multocida.

A group of nine bovine Pasteurella strains not producing indole were investigated for their taxonomic relationships with Pasteurella multocida, Pasteurella haemolytica and Pasteurella canis. For all strains, DNA-DNA hybridization has revealed a high genetic relatedness at the species level to P. multocida and significantly lower homologies of only 18-41% towards P. haemolytica and 11-15% towards P. canis. Guanine plus cytosine values of 38.0 to 42.1 mol% and several phenotypic characters have been found to be different from the established pattern for P. multocida subspecies. It is suggested that the strains represent a new taxon, possibly another P. multocida subspecies.

Animals↗
PubMed1989

Changes in the relative concentrations of surfactant phospholipids in young pigs with experimental pneumonia.

Lung lavage fluids of 23 young pigs were investigated prior to and after experimental infection with Pasteurella multocida. Comparison of the phospholipid patterns showed an increase in the relative concentration of phosphatidylinositol and a decrease in that of phosphatidylglycerol in the diseased animals. The phosphatidylcholine-to-phosphatidylinositol and phosphatidylglycerol-to-phosphatidylethanolamine ratios were used as parameters to characterize the changing patterns. The reductions in these ratios following infection were found to be useful indicators of bacterial pneumonia. Immunization did not affect the characteristic variations. A rapid screening procedure involving solid-phase extraction, one-dimensional thin-layer chromatography and densitometric scanning of the plates was used.

Animals↗
PubMed1988

Characterization of the agent of swine dysentery based on deoxyribonucleic acid homology.

Chromosomal deoxyribonucleic acids (DNAs) of five strains formally assigned to Treponema hyodysenteriae and Treponema innocens have been compared. The results of DNA-DNA-hybridization indicate that all the strains belong to the same species. The very low guanine-plus-cytosine values between 24.7 and 29.7 mol% are regarded as further evidence in favour of the reclassification of the swine dysentery agent as Borrelia hyodysenteriae (comb. nov. Blaha).

Animals↗
PubMedDate not supplied

[Improvement and acceleration of the diagnosis of contagious bovine pleuropneumonia by direct detection of the microbe using polymerase chain reaction (PCR)].

A diagnostic procedure is described for the detection of Mycoplasma mycoides subsp. mycoides SC, the causative agent of contagious bovine pleuropneumonia. DNA extracted from clinical samples was investigated by the polymerase chain reaction (PCR) using at least 2 different primer pairs, one species-specific and another one specific for the class of Mollicutes. Using this method, the time required for detection of the pathogen was reduced to 2 days, whereas with traditional diagnostic methods (cultivation in broth, biochemical tests or immunofluorescence) the same finding would be available only within approximately 20 days. Although contagious bovine pleuropneumonia does not occur in Central Europe, there are occasional identifications of cattle having positive titres in the complement fixation test (CFT). Immunoblotting analysis of such sera confirmed that the reason for this phenomenon were cross-reactions with taxonomically related mycoplasma species. The present PCR assay proved to be suitable because of its rapidity, as well as high specificity and sensitivity. In the case of positive serological findings it enables diagnosticians to provide evidence on the presence or absence of the agent at short notice.

Animals↗