Search PubMed⌕ Search

Biomedical subjects

K S Stenn

Publications and source records attributed to K S Stenn.

At least 73 records · Page 4Linked to original sources
PubMed1983

Patterns of congenital nevocellular nevi. A histologic study of thirty-eight cases.

A series of thirty-eight cases of congenital nevocellular nevi were excised and studied histologically. Depending on the extent of nevoid cell dermal infiltration, four histologic patterns were observed: diffuse or patchy infiltration of the upper dermis alone, or diffuse or patchy infiltration of the upper and deep dermis with nevoid cells in or below the lower one third of the reticular dermis. Nevoid cells extended into the deepest reticular dermis in only 37% of these patients. There was no significant correlation between the four histologic patterns and age, sex, location, or lesion size. Two cases recurred after surgery but none underwent malignant change. The patients who developed recurrent nevi in the surgical site postexcision manifested the diffuse upper and deep dermal pattern of nevoid cell infiltration. We interpret this observation as due to incomplete excision of the primary lesion, reflecting the difficulty of removing congenital nevi with this histologic pattern. We conclude from this study that: (1) congenital nevocellular nevi show at least four typical histologic patterns; (2) the nevoid cells of congenital nevi do not necessarily extend into the deep dermis; and (3) of the four histologic patterns seen in congenital nevi, that one showing diffuse and deep placement of nevoid cells on biopsy requires more aggressive excision to prevent recurrence.

Adolescent↗
PubMed1983

Multiple mechanisms of dissociated epidermal cell spreading.

To test the possibility that epidermal cells use a common basement membrane protein whenever they spread, in vitro experiments were conducted using trypsin-dissociated guinea pig epidermal cells and the following proteins: human serum, bovine serum albumin, serum fibronectin, Type IV collagen, laminin, and epibolin (a recently described serum glycoprotein which supports epidermal cell spreading; Stenn, K.S., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:6907.). When the cells were added to media containing the specific proteins, all the tested proteins, except for serum albumin, supported cell spreading. Added to protein-coated substrates in defined media, the cells spread on fibronectin, epibolin, and laminin-Type IV collagen, but not on albumin or whole serum. In none of these experiments were the results qualitatively affected by the presence of cycloheximide. Antibodies to a specific protein blocked cell spreading on that protein but not on the other active proteins, e.g. whereas antibodies to epibolin blocked cell spreading on epibolin, they did not affect spreading on fibronectin, collagen, or laminin. In a second assay in which the cells were allowed to adhere to tissue culture plastic before the protein-containing medium was added, the cells spread only if the medium contained epibolin. Moreover, under these conditions the spreading activity of whole serum and plasma was neutralized by antiepibolin antibodies. These results support the conclusion that dissociated epidermal cells possess multiple spreading modes which depend, in part, on the proteins of the substrate, proteins of the medium, and the sequence of cell adhesion and protein exposure.

Animals↗
PubMed1983

The early clinical manifestations of Lyme disease.

Lyme disease, caused by a tick-transmitted spirochete, typically begins with a unique skin lesion, erythema chronicum migrans. Of 314 patients with this skin lesion, almost half developed multiple annular secondary lesions; some patients had evanescent red blotches or circles, malar or urticarial rash, conjunctivitis, periorbital edema, or diffuse erythema. Skin manifestations were often accompanied by malaise and fatigue, headache, fever and chills, generalized achiness, and regional lymphadenopathy. In addition, patients sometimes had evidence of meningeal irritation, mild encephalopathy, migratory musculoskeletal pain, hepatitis, generalized lymphadenopathy and splenomegaly, sore throat, nonproductive cough, or testicular swelling. These signs and symptoms were typically intermittent and changing during a period of several weeks. The commonest nonspecific laboratory abnormalities were a high sedimentation rate, an elevated serum IgM level, or an increased aspartate transaminase level. Early Lyme disease can be diagnosed by its dermatologic manifestations, rapidly changing system involvement, and if necessary, by serologic testing.

Abdomen↗
PubMed1982

Aortic endothelial cell migration. I. Matrix requirements and composition.

Endothelial cell migration was studied following a mechanical injury produced in cultured confluent monolayers of calf aortic endothelium with the use of a quantitative migration assay. In this method the cells were grown on glass coverslips coated with scarlet red-containing Formvar. At confluency, the cultures were cut in half with a blade; one half was removed with the pigmented Formvar, and the other was returned to culture. Migration was linear for a least 96 hours, and was due to cell motility, not proliferation. Since it was blocked in the presence of L-azetidine carboxylic acid or cis-hydroxyproline, inhibitors of collagen secretion, endothelial cell migration appeared to be dependent on the continual secretion of collagen. Furthermore, the types, apparent relative amounts, and localizations of the collagens as well as laminin changed during the migratory process. These studies support the notion that the aortic endothelial cell migratory response to injury is a dynamic one requiring the continual secretion and modulation of matrix molecules.

Animals↗
PubMed1981

An unusual and fatal case of disseminated cutaneous herpes simplex. Infection in a patient with cutaneous T cell lymphoma (mycosis fungoides).

A patient with plaque stage mycosis fungoides (MF) developed an atypical disseminated cutaneous herpes simplex virus (HSV) infection manifested by polycyclic cutaneous ulcers. Although Tzanck preparations and serial antibody titers to herpes virus were negative, the diagnosis was readily established by viral culture and histologic examination of the skin lesions. Following adenine arabinoside therapy, the viral cultures of the ulcers became negative and the spread of virus-induced ulcerations ceased. In an immunocompromised host with rapidly advancing, sharply punched-out polycyclic cutaneous ulcerations, herpes simplex infection should be considered even though the classical vesicular lesions are absent.

Female↗
PubMed1981

Spongiosis.

Explore the source record for details and available documents.

Edema↗
PubMed1981

Epibolin: a protein of human plasma that supports epithelial cell movement.

Earlier studies suggested that there is a specific activity in mammalian serum and plasma that supports epidermal (epithelial) cell movement. This activity was shown to be nondialyzable and heat labile. In the studies reported here, using standard biochemical procedures--i.e., ammonium sulfate fractionation, ion-exchange and gel filtration chromatography, isoelectric precipitation, and preparative polyacrylamide gel electrophoresis--we have purified a factor from human plasma that supports epidermal cell movement. The factor travels as an apparent single band on disc gel electrophoresis and corresponds to a glycosylated single-chain protein of approximately 65,000 +/- 3,000 daltons. The purified fraction is necessary and sufficient for dissociated epidermal cells, (ii) outgrowth of epithelial sheets from skin explants, and (iii) epiboly, epithelial sheet movement over a floating skin explant. The purified fraction is active at a concentration of 1-2 micrograms/ml of growth medium. It is destroyed by trypsin and its activity is augmented more than 10-fold by a second, as yet unpurified, fraction of plasma. These studies support the notion that a single protein of plasma supports epidermal cell movement and that this protein may play an important role in wound closure. Because it supports epiboly, the most biologically relevant of the assays, it has been named epibolin.

Cell Movement↗
PubMed1980

Clinicopathologic relationships, survival, and therapy in 59 patients with observations on occupation as a new prognostic factor.

Clinicopathologic relationships, survival and therapy were reviewed in 59 patients with mycosis fungoides (MF). An analysis of patient survival disclosed that stage of disease was a significant prognostic variable only if both cutaneous and visceral manifestations were considered in the staging design. The classical three-stage format, based solely on findings of eczema (I), plaques (II), or skin tumors (III), was not a significant factor in predicting survival. However, the inclusion of lymphadenopathy (IV) and organomegaly (V) or circulation Sézary cells (VI) in an expanded model revealed a significant decline in the probability of survival with increasing stage of disease. Regarding cases where the original histopathologic material was available for review, there was no association between the histologic stage of the specimen and the morphology of skin lesions. These data militate against the use of a staging scheme based on histologic criteria. Among 30 different types of treatment employed during the course of this study, highdose electron beam was superior to all other physical and chemotherapeutic modalities. In a case-control study considering occupational factors, patients with MF who were employed in manufacturing or construction industries were at significantly increased risk (relative risk = 4.3). Patient survival was reduced considerably for those with industrial backgrounds, suggesting that this subgroup was inclined to have severe disease. The concept that occupational factors may be implicated in the etiology of mycosis fungoides provides a new dimension to previous pathogenic hypotheses that needs further evaluation.

Adolescent↗
PubMed1980

Quantitative assay of dissociated tissue-cell motility in vitro.

A simple method for quantitating the motile properties of a dissociated tissue cell preparation is presented. A cell population is allowed to grow over a glass cover slip coated with Scarlet Red-containing Formvar. At confluence, the preparation is cut with a razor to remove a portion of the cells and the underlying pigmented Formavar and then returned to culture conditions. Using the cut edge as the starting line, cell motility can be easily measured. In this assay irradiated and nonirradiated 3T3 cells show similar motility characteristics over a 3-day observation period suppirting the interpretation that the observed movement is due primarily to active cell motility rather than cell growth.

Animals↗
PubMed1980

Fibronectin (LETS) does not support epithelial cell spreading.

The dependence of epithelial cell spreading on fibronectin-containing media has been investigated, using purified human plasma fibronectin, and two in vitro assays: (1) epidermal outgrowths from measured pieces of mouse ear skin, and (2) spreading of dissociated cells from trypsinized guinea pig epidermis. Using these systems, appreciable spread occurred in media containing whole plasma and plasma deficient in fibronectin but no significant spread occurred in media enriched only in fibronectin. Returning fibronectin to deficient plasma did not enhance or reduce optimal cell spreading. These studies suggest that the factor(s) in human plasma which supports epithelial cell spreading in vitro is not fibronectin and that the chemistry of epithelial and fibroblast cell spreading is different.

Animals↗
PubMed1979

Human serum and epithelial spread in tissue culture.

The dependence of epithelial spread on human serum in culture has been studied. Using measured pieces of mouse ear skin epithelial outgrowth about floating explants (epiboly) and from adherent explants was studied. Where compared directly the two systems show similar results. Because of its ease of quantitation, the adherent explant culture was studied in greater detail. In this system in the presence of serum appreciable spread was found only after 48 h but spread continued for at least the next 4 days. In the absence of serum only minimal epithelial spread occurred. Adding serum to deficient media enhanced spreading and removing serum from media depressed spreading. The extent of spread appeared independent of mouse age for the first 4-10 weeks though 2-week-old mouse skin showed quantitatively greater spreading. The activity in human serum responsible for epithelial spread acts under conditions of minimal DNA synthesis and is not reproduced by bovine serum albumin, fetuin, or serum that had been exposed to 100 degrees C for 5 min. The activity is not dialyzable and it is resistant to the protease inhibitors DFP and PMSF. These studies suggest that a specific serum component(s) serves to support epithelial spread in vitro.

Age Factors↗
PubMed1979

Epidermal hyperplasia induced in guinea pig flank skin by intradermal injection of Sudan red.

Studies of dermal-epidermal interactions were conducted with guinea pig flank skin and intradermal injections of the irritant, Sudan IV dye in olive oil. These injections led to epidermal hyperplasia in areas overlying the irritant and the effect was most significant when the irritant was placed in the upper dermis. Basal cell mitotic activity and thymidine uptake reached a peak by 24 h and thereafter dropped rapidly. Maximal epidermal thickness (4.3 times the control) resulting from an increase in cell number occurred within 2-4 days. Despite the very short period of increased cell growth, epidermal thickness returned to control values only after a 24-day period. A similar growth response could not be induced by saline injections. A single topical application of the irritant showed a qualitatively and quantitatively different epidermal response. These experiments indicate that an intradermal irritant can lead to epidermal hyperplasia and a long-lasting epidermal thickening.

Animals↗
PubMed1978

Metastasizing atypical fibroxanthoma. Coexistence with chronic lymphocytic leukemia.

Atypical fibroxanthoma (AFX) is one of a group of cutaneous lesions with a malignant histological appearance but a generally benign clinical course. A 79-year-old white man had AFX of the cheek that recurred and metastasized to buccal and cervical lymph nodes three months after initial diagnosis. When careful physical and and laboratory examinations were done, the patient was found to have concomitant chronic lymphatic leukemia, "null cell" type. In view of the low incidence of metastasizing AFX and the increased occurrence of tumors in patients with lymphomatous disorders, an important association is suggested. Before establishing the prognosis for patients with pseudomalignancies of the skin, an evaluation of their general health and immunological status should be made.

Aged↗
PubMed1978

The role of serum in the epithelial outgrowth of mouse skin explants.

An experimental system is described which facilitates the measurement of epithelial outgrowth in vitro. Using this sytem the properties of the serum component supporting epithelial spread have been studied. It has been found that appreciable epithelial outgrowth occurs in concentrations of cow serum greater than 0.5% and that maximal outgrowth occurs at concentrations of 1% or greater. Only minimal outgrowth is apparent during the first day of culture. The lack of epithelial outgrowth in the absence of serum is not due to reduced cell viability since replacing serum after 3 days leads to cellular outgrowth. Though this activity is found in several sera studied (cow, fetal calf, human, horse, mouse and chicken), there are considerable quantitative differences between species. Epithelial cell outgrowth does not occur in the presence of medium plus bovine fibrin, serum albumin or thrombin. The serum component is heat labile and non-dialysable. It appears to be needed continuously and does not require DNA synthesis for its effect.

Animals↗
PubMed1976

Organ culture of adult mouse esophageal mucosa in a defined medium.

A method is described for culturing adult mouse esophageal mucosa in a chemically defined medium. The preparation consists of mucosa and superficial submucosa. By light microscopy, autoradiography, and tritiated-thymidine [3H]TdR) uptake the tissue appears viable for at least 3 days. Although a drop in the rate of [3H]TdR uptake is observed in the initial hours of culture, recovery occurs by 24 hr and uptake remains constant for at least an additional 48 hr. Twenty-four-hour exposure to [3H]TdR and autoradiography reveals that 93 +/- 3% of the basal cells take up label; appreciable labeling is not found in other cells of the preparation. Pulse labeling indicated a transit time from basal layer to keratin layer of about 72 hr. This preparation should be useful for short-term in vitro studies of keratinized, stratified, squamous epithelium free of appendages and for studies of the growth properties of esophageal mucosa under simulated pathologic conditions.

Animals↗