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Biomedical subjects

K S McCarty

Publications and source records attributed to K S McCarty.

At least 109 records · Page 6Linked to original sources
PubMed1980

Sex steroid receptor analysis in human melanoma.

Melanomas from 20 patients were evaluated for estrogen and progesterone receptors. No restriction as to patient's age, sex, race, or menstrual status was made. Fifteen of the tumors were melanin producing. Of the 20 tumors examined, seven contained more than 3 fm/mg protein of specifically inhibitable estrogen binding. None of the tumors reported here demonstrated either a 4S or 8S binding protein as shown by sucrose density gradient analysis. All tumors in which estradiol binding was observed were melanin producing, whereas none of the amelanotic melanomas (five tumors) bound this steroid. Purified tyrosinase appeared to mimic the estrogen binding detected in melanoma cytosols, as demonstrated with the dextran-coated charcoal technique. Although the binding of estradiol to tyrosinase was inhibited by DOPA, the binding of estradiol to estrogen receptor preparations was not. These studies represent an extensive of previous studies of sex steroid binding in melanomas and suggest that gradient analysis and DOPA inhibition studies should be included in the evaluation of the estrogen binding phenomenon in human melanoma.

Adult↗
PubMed1980

Conservative management of the Zollinger-Ellison syndrome. Ectopic gastrin production by an ovarian cystadenoma.

Techniques for evaluating ectopic gastrin production associated with the Zollinger-Ellison syndrome are described. The site of gastrin production in this example was a mucinous cystadenoma of the ovary confirmed by immunoperoxidase localization of gastrin within the ovarian tumor cells and by radioimmunoassay of the tumor's gastrin content. These methods, localizing the source of ectopic gastrin, permitted conservative management of the ulcer diathesis with surgical resection of the mucinous cystadenoma. After oophorectomy the patient's serum gastrin level returned to normal, and the ulcer diathesis ceased.

Cystadenoma↗
PubMed1980

Malignant effusions in recurrent breast cancer: steroid hormone receptor analysis of effusion fluid derived cells.

Estrogen and progesterone receptor determination in breast cancer is of significance in determining a patient's potential response to hormonal manipulation. One practical limitation to this procedure has been availability of material for receptor assay. We describe 12 patients in whom quantitative estrogen and/or progesterone receptor analysis was accomplished utilizing tumor cells obtained from malignant effusions. The ability to obtain quantitative steroid receptor data on material derived from malignant effusions provides an effective source for this important biochemical parameter in patients in whom no other tumor tissue is available for study.

Adult↗
PubMed1980

Tonsillar metastasis from carcinoma of the breast with ultrastructural and steroid receptor analyses.

An unusual site of metastasis for mammary carcinoma is presented with an examination of the tumor's histologic and ultrastructural features, as well as its estrogen and progesterone receptor protein content. The importance of careful examination of the tonsillar area is emphasized in this patient in whom the tonsillar metastasis represented the initial indication of breast tumor recurrence. Estrogen and progesterone receptor analyses are utilized both as an aid in identifying the origin of the metastatic tumor focus as well as an aid in selecting therapy.

Adult↗
PubMed1980

Clinical correlates of estrogen- and progesterone-binding proteins in human endometrial adenocarcinoma.

Seventy-eight endometrial carcinoma specimens from 74 patients were evaluated for estrogen (E2R) and progesterone (PrR) receptors by multiconcentration saturation and sucrose density gradient analysis. The clinical stage and histologic grade of the tumors and the patients' clinical course were compared to the qualitative and quantitative evaluations of E2R and PrR. Although no correlation was seen between receptor content and extent of disease, tumors with significant levels of E2R and PrR tended to be less aggressive than those in which neither receptor was detected. The histologic grade correlated with a combination of estrogen and progesterone receptor content. In 92% of the patients there was a concordance between the presence or absence of E2R and PrR in the tumor and a clinical response to progestin therapy. The significance of these findings in relationship to the presence of multiple forms of steroid binding is discussed.

Adenocarcinoma↗
PubMed1980

Hormone responsiveness of a transplantable rat chondrosarcoma. II. Evidence for in vivo hormone dependence.

We have previously demonstrated that the Swarm rat chondrosarcoma responds to GH-dependent serum factors in vitro by increasing amino acid transport and macromolecular synthesis. The question of in vivo hormone dependence was evaluated by studying the growth of the tumor in hypophysectomized rats. Tumor-bearing hypophysectomized rats were treated with saline, T4, cortisone, bovine GH (bGH), or combinations of these hormones. Tumor growth was assessed in terms of tumor weight. Histological appearance was studied to ascertain the viability of the tumors and the relative contributions of cellularity vs. cartilage matrix to the weight of the chondrosarcoma. The weight of tumors grown in saline-treated hypophysectomized rats was less than 10% of the weight attained by tumors grown in normal rats for a comparable period of time. There was a greater relative cellularity in tumors grown in normal and hormone-treated hypophysectomized rats compared to that in tumors grown in saline-treated hypophysectomized rats. Tumors from saline-treated hypophysectomized rats had an atrophic appearance. Treatment of the hypophysectomized rats with bGH or cortisone increased the rate of tumor growth 5- to 6-fold and restored the histological appearance toward that of tumors grown in normal rats. A greater rate of tumor growth was effected by treatment with a combination of bGH and cortisone. T4 by itself did not stimulate chondrosarcoma growth in the hypophysectomized rat. Withdrawal of bGH and cortisone treatment from hypophysectomized rats after tumors were hormone stimulated caused a loss of the apparent exponential rate of growth observed in hypophysectomized rats treated through the full 38-day observation period. These data indicate that bGH and cortisone act in concert to stimulate the growth of the Swarm rat chondrosarcoma in vivo and that the tumor is hormone dependent.

Animals↗
PubMed1980

Chloroma (granulocytic sarcoma) without evidence of leukemia: facilitated light microscopic diagnosis.

Localized tumors composed of immature cells of the myelogenous series have been recognized for many years as an uncommon manifestation of granulocytic leukemia. The histologic diagnosis of chloroma (granulocytic sarcoma) may be extremely difficult when the myeloblastic cells are poorly differentiated and the tumor lacks the characteristic green color. The diagnostic difficulty may be further compounded when the granulocytic sarcoma develops before there is peripheral blood or bone marrow evidence of leukemia. Previous criteria for the diagnosis of chloroma have been ambiguous because of the capricious nature of the hydroperoxidase activity and the lack of definitive histochemical criteria. In this case, a combination of Sudan black B and myeloperoxidase histochemical staining and ultrastructural evaluation was applied. The light microscopic histochemical studies suggested the presence of Phi bodies and rods both in the formalin-fixed tumor and in the cells derived from the subsequent pleural effusion; this was confirmed by electron microscopy, which demonstrated the peridicity of the crystalline rod substructure. These observations show that light microscopic histochemical studies can facilitate the diagnosis of granulocytic sarcoma or chloroma in the absence of peripheral blood or bone marrow manifestations of leukemia.

Adult↗
PubMed1980

Adenosquamous differentiation in mammary carcinoma: an ultrastructural and steroid receptor study.

Five breast carcinomas with only squamous morphology by light microscopy demonstrated ultrastructural features of both squamous and adenomatous differentiation within the same individual cells. Two of the five cases demonstrated quantitatively low levels of saturable estrogen- and/or progesterone-binding proteins. In no case were there quantitative or qualitative patterns of receptors indicative of clinical hormonal response potential. It is suggested that primary squamous cell carcinomas of the breast should be examined ultrastructurally for adenosquamous differentiation as each of the five apparent squamous carcinomas observed in a prospective study of 1,760 (0.3%) cases of breast cancer demonstrated adenosquamous differentiation ultrastructurally.

Adenocarcinoma↗
PubMed1980

Adenocarcinoma of Bartholin's gland.

Bartholin's gland carcinoma is an uncommon disease representing 0.1% of all female genital malignant neoplasms. Five Bartholin's gland adenocarcinomas were selected by the criteria of Chamlian and Taylor. Three patients died from metastatic disease within four years; two are free of apparent disease 14 months and 13 years, respectively, after initial diagnosis. Poor prognosis was associated with large tumor size, poor histopathologic differentiation, and lymph node involvement. Transmission electron microscopy verified the glandular nature of the poorly differentiated lesions. All five tumors demonstrated junctional complexes, abundant rough endoplasmic reticulum, secretory vacuoles, and glandular formation. Low levels of estrogen receptor and moderate levels of progesterone receptor were present in the one case measured. Endogenous peroxidase, and inducible enzyme in estrogen-sensitive tissues, was observed in two of the five tumors.

Adenocarcinoma↗
PubMed1980

Selection of patients for heterotopic implantation of the areola and nipple.

In a prospective analysis of 291 specimens taken at mastectomy for carcinoma, 48 contained tumor in the nipple-areola complex. The characteristics of greater tumor size, higher frequency of lymph node involvement, midbreast tumor location and multifocality of tumor correlated well with the probability of involvement of the nipple. The findings emphasize the importance of close clinical and histopathologic examination of the nipple and areola when preservation of the nipple is being considered.

Adult↗
PubMed1979

Estrogen receptor determination in percutaneous bone marrow biopsies of patients with metastatic breast cancer.

Estrogen receptor determination in metastatic breast cancer has been shown to be important in determining potential for response to endocrine therapy. One limitation to this procedure can be the availability of neoplastic material for receptor assay. This report describes patients in whom estrogen receptor status was determined using malignant cells obtained through routine posterior iliac crest bone marrow biopsy. The ability to obtain receptor data from percutaneous bone marrow biopsy specimens has proved to be of important in patients in whom other sites involved with tumor were not accessible.

Adult↗
PubMed1979

Cystosarcoma phylloides. A steroid receptor and ultrastructure analysis.

Six cases of cystosarcoma phylloides were evaluated by ultrastructure and steroid receptor analysis. Electron microscopy of the lesions supported previous reports of a heterogeneous tumor consisting of pleomorphic mesenchyme and normal or proliferative epithelium. In each case estrogen and progesterone receptor analysis indicated the presence of a nonsaturable estrogen and progesterone 4S binding protein rather than a specific steroid receptor as suggested by previous studies.

Adolescent↗
PubMed1979

Correlation of estrogen and progesterone receptors with histologic differentiation in endometrial adenocarcinoma.

Endometrial carcinomas from 58 patients were graded histologically, and the histologic grade was compared with the estrogen and progesterone receptor content of the tumor tissues. Receptor content was determined by multiple concentration saturation analysis and sucrose density gradient analysis. A positive correlation was found between the presence of estrogen and progesterone receptors and the degree of tumor differentiation. The majority (85%) of well-differentiated lesions contained significant amounts of both steroid receptor proteins. In contrast, only 13% of the poorly differentiated lesions were characterized by the presence of detectable levels of both estrogen and progesterone receptor proteins. The relationship of receptor content and degree of differentiation to prognosis and potential for response to hormonal therapy is discussed.

Adenocarcinoma↗
PubMed1978

Gonadal dysgenesis with adenocarcinoma of the endometrium: an electron microscopic and steroid receptor analyses with a review of the literature.

A patient with gonadal dysgenesis who developed endometrial carcinoma in her 31st year of estrogen therapy is discussed in relation to thirteen previously reported cases of carcinoma of the uterus in patients with Turner's syndrome. The pattern of steroid receptor proteins in the endometrial carcinoma was found to correlate with the degree of differentiation of the tumor as assessed by light and electron microscopy. The findings reflect a potential for continued hormonal responsiveness of the tumor.

Adenocarcinoma↗
PubMed1978

Thymolipoma in association with myasthenia gravis.

A patient with a 100-g thymolipoma and myasthenia gravis experienced marked improvement in neuromuscular symptoms following removal of the tumor. Although 60 examples of thymolipoma have been reported, we believe that this is the first example of this tumor associated with myasthenia gravis. The clinical, radiologic, and histopathologic manifestations of previously reported cases of thymolipoma are discussed in relation to our patient's case.

Humans↗
PubMed1978

Histopathologic study of subcutaneous mastectomy specimens from patients with carcinoma of the contralateral breast.

Fifty subcutaneous mastectomy specimens, each of which were removed from a patient who had previously undergone radical or modified radical mastectomy of the contralateral breast for carcinoma, were age and date matched to a control group of patients with which carcinoma was not associated. The patients were evaluated with regard to specific types of epithelial proliferative lesions and other entities of fibrocystic disease without knowledge of their group membership. The results included a statistically significant dominance of certain epithelial hyperplasias in the study population and a lack of statistical significance in intergroup difference of some previously suspected lesions, such as sclerosing adenosis and gross cystic disease. It is concluded that the recognition of those forms of epitheliosis associated with carcinoma may help in the management of patients at high clinical risk for carcinoma of the breast.

Adult↗