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Biomedical subjects

K Rytwiński

Publications and source records attributed to K Rytwiński.

At least 19 recordsLinked to original sources
PubMed1996

[The place and role of health promotion in oncology].

At the present time, groups people who live with chronic illness or outcome of medical intervention eliminated the disease process are in greater and greater number. It concerns cancer-patients and their life- complication too. Innovative approaches towards to health promotion in this field is actual and important. Recent research documents a number of cases of the effectiveness of psycho-oncological intervention and social support on the clinical course of cancer and help prolong life. Actual experience and results of psychosocial programmes, to stress-inhibition, quality of life, physical well-being (so long as possible), in the context a categorization of target groups of cancer patients, course of disease, medical treatment and relation of health promotion to cancer prevention problems, were discussed. It was partially estimated in relation to problems of health promotion concerning cancer-patients in Poland.

Chronic Disease↗
PubMed1992

[Anemia and recurrences in acute lymphoblastic leukemia in children after the treatment and the frequency of the erythroblasts with micronuclei].

A retrospective analysis of the relation of relapses and anaemias to the frequency of micronucleated erythroblasts in bone marrow smear in 140 children with acute lymphoblastic leukaemia after BFM and Memphis schemes of therapy was carried out. The anaemias correlated with the frequency of micronucleated erythroblasts in statistically significant manner. Correlation concerning relapses, was not shown. The frequency of micronucleated erythroblasts in children treated by BFM scheme is lower than by Memphis scheme. This suggests smaller genotoxicity of BFM scheme therapy.

Adolescent↗
PubMed1989

[Selected immunologic parameters and infections in children in over 1 year after completion of the treatment of acute lymphoblastic leukemia].

An analysis of infection incidence within one year after completion of the treatment for the acute lymphoblastic leukemia was performed in the group of 24 children. Infections incidence was related to the selected immunological parameters, mainly T4 and T8 lymphocyte subpopulations assayed with monoclonal antibodies. T4/T8 cells ratio (Ix) was determined. It was found that moderate decrease in the total lymphocyte count and Ix exist in children one year after completion of the treatment. This index is more significantly lower in children with more frequent infections while the absolute numbers of T4 and T8 lymphocytes are relatively increased. The obtained results suggest that no significant immunosuppression is observed in children affecting the number and the course of infections.

Bacterial Infections↗
PubMed1988

Cross-reaction of 791T/36 monoclonal antibody with immunological activated human peripheral blood mononuclear cells.

Monoclonal antibody 791T/36, directed against surface antigen on the human osteosarcoma cell line (791T), and cross-reacting with surface antigen p72 expressed on activated (PHA, MLR) human peripheral blood mononuclear cells (PBM), was used in analysis of p72 antigen in vitro. Using FACS-IV system and fluorescence microscope, it was shown that expression of p72 antigen on PBM cells is dependent on phytohaemagglutinin (PHA) stimulation, and alloantigens in mixed lymphocyte reaction (MLR). The increase of p72 expression is always connected with lymphocytes proliferation activity (FACS-IV analysis and 3H-Thymidine incorporation assay). The expression of p72 is not significantly dependent on cell size, and stage of the cell in the cell cycle. A suggestion, that p72 is a one of so-called "proliferation antigens" is discussed. Present study emphasizes a problem of cross-reactivity in the context of possible errors in diagnosis and therapy, when a monoclonal antibody cross-reacts with cells other than intendent target cells.

Antibodies, Monoclonal↗
PubMed1984

Analysis of surface antigen expression per cell of human osteogenic sarcoma cells by fluorescence-labelled monoclonal antibodies.

The relationship between total surface antigen expression per cell (means) - measured by fluorescence-labelled monoclonal antibodies (fluorescence-histograms) and the distribution of cells in the cell cycle (DNA-histograms) and size-scattergrams (cell sorter FACS-IV) were analysed in drug treated unsynchronized and synchronized osteogenic sarcoma cells (2OS) in vitro. Drugs with various sites of action in the cell cycle were used. Adriamycin, Vindesine, in concentrations applied accumulate cells in G2 + M phase. Methotrexate arrests cells in the boundary of G1/S phase. Size-scattergram and DNA-histogram analysis have shown that the entrance of cells to the cell cycle is usually accompanied by an increase in the cells size and amount of their DNA. The size of the cells influenced antigenic expression much more than the distribution of the cells in the various cell cycle phases: in the bigger cells the expression per cell was more pronounced. The increase of antigen expression was the highest for Adriamycin and for Methotrexate treated cells. However, this increase was limited and never exceeded plus 50% in relation to the control. This relatively low difference resulted from the fact, that a given phase of the cell cycle included cells markedly heterogenic in respect of size and antigenic content. It was also shown that lower concentration of serum in culture medium and confluent growth of older cultures decrease surface antigen expression per cell.

Animals↗
PubMed1981

Adoptive immunotherapy and combined chemo-immunotherapy in transplantable leukaemia of AKR mice (TAL); occurrence of natural killers.

Allo- and syngeneic lymphoid effector cells were injected I. P. into transplantable leukaemia (TAL)-bearing AKR recipients. Effector cells were collected from the peritoneal cavity, lymph nodes, spleen, or thymus, of either non-immunized donors or of donors previously immunized with TAL cells. The ratio, injected number of effector cells divided by injected number of TAL target cells varied in individual experiments from 10:1 to 10 000 : 1. Some AKR recipients were given prior to the leukaemia inoculation a whole-body sublethal X-ray irradiation and/or an I.P. injection of cyclophosphamide. Mean survival of TAL leukaemia was most often prolonged in recipients given viable allogeneic thymocytes derived from non-immunized donors. In some leukaemia-bearing mice treated with chemo-immunotherapy, permanent survival (greater than 70 days) could be observed. As a rule, effector cells derived from non-immunized allogeneic donors were more effective than effector cells from the immunized areas. Syngeneic effectors were ineffective. The occurrence of natural killers (NK cells) to Gross virus-infected cells in the lymphoid organs of low-leukaemic inbred mouse strains is postulated. These NK cells are lacking in high-leukaemic AKR mice.

Animals↗
PubMed1979

Adoptive immunotherapy and chemo-immunotherapy in murine L-1210 leukemia and its influence on the kinetics of leukemic proliferation.

Donor allogeneic C57Bl/6 lymphoid cells from peritoneal cavity, lymph nodes, thymus and spleen of immunized and non-immunized mice were used for adoptive immunotherapy of L-1210 ascites leukemia in DBA/2J recipients. Donor effector cells were injected i.p. into leukemic mice which were given a whole-body irradiation in a dose of 300 r X-rays prior to leukemia inoculation. The in vitro cytotoxicity of the effector cells was tested by the LT- and 51Cr-tests. Cytokinetics of leukemia undergoing therapy was followed by impulse-cytophotometry. An adoptive chemo-immunotherapy with the aid of peritoneal or splenic lymphoid cells from immunized donors proved to be most effective. The cells were injected on the second day into recipients inoculated with leukemia on the day 0, and then given on the first day a single i.p. injection of cyclophosphamide in a dose of 50 mg/kg body weight. Under these conditions the secondary disease did not influence therapy and apart from a distinctly prolonged survival there was also noted a number of permanent survivals of leukemia. It was also found that peritoneal and splenic lymphoid cells of immunized donors exhibited most pronounced direct and indirect cytotoxicity against target cells in vitro. In vivo, the effector cells mounted an attack on the L-1210 cells probably at the onset of the G1-phase of the cell cycle.

Animals↗
PubMed1976

[The acid-base equilibrium during mouse lymphoblastic leukemia].

In transplantable and in spontaneous lymphoblastic mouse leukemia blood pH, PCO2, TCO2 and BB were examined. Spontaneous Gross-leukemia in AKR mice was found to develop in its final phase respiratory acidosis. Transplantable (TAL) leukemia of AKR mice presents from the onset a tendency toward respiratory acidosis. "L-1210"-leukemia, on the contrary, was shown to alcalise the recipients during the first 8 days after inoculation, later on it can bring about an acidosis. The pH-deviations in "L-1210"-leukemia display a respiratory character in syngeneic DBA/2J recipients, whereas in semiallogeneic recipients the observed changes are metabolic or mixt in nature. This finding strongly argues for the importance of histocompatibility. Intranodally inoculated animals distinctly differ in their parameters in comparison to intravenously and intraperitoneally recipients, hence, an important role of the tissular-milieu which is put first in contact with the leukemic factor must be concluded.

AKR murine leukemia virus↗
PubMed1976

[The effect of exogenous alkalization on cytokinetics and on the survival rate of mice with lymphoblastic leukemia].

In mice vaccinated with two forms of lymphoblastic leukaemia and alkalized with intravenous administration of sodium bicarbonate, the survival rate, the extent of leukaemic infiltration and the proliferative capacity of cells in the bone-marrow, thymus, spleen, lymphnodes, liver and lungs were investigated. The survival rate in the TAL leukaemia of the AKR stem producing an endogenous acidosis could be significantly prolonged in a statistical way by alkalization. Yet an accelerated expiring rate could be observed after exogenous alkalization in L-1210 leukaemia of the DBA/2J stem producing an endogenous alkalosis. By means of cytological and impulse-cytophotometrical investigations the exogenous alkalization of both forms of leukaemia could be proved to have a direct bearing on the proliferative kinetics. In TAL leukaemia the leukaemic proliferation was inhibited by the exogenously involved correction of the acid-base balance; in the L-1210 leukaemia, however, the pH disturbances were enhanced, thus accelerating the leukaemic proliferation. Consequently, the disturbances of the acid base balance seem to be an essential cofactor in the leukaemia genesis. The exogenous direction of the acid-base balance may be important as a means of treating leukaemia.

Animals↗
PubMed1975

[A case of atypical myeloblasts; a RNA leukemia].

In a 53-year-old female patient, a tumor localized in the nasopharyngeal cavity together with hematologic features of acute paramyeloblastic leukemia were observed. More than 70% blast cells contained giant intracytoplasmic inclusions which have been found to be strongly pyroninophilic. The electron-microscopic study revealed big agglomerations of ribosomal RNA inside of pseudofibrillar structures of the cytoplasma. The possibility of a new nosological entity should be envisaged.

Bone Marrow↗
PubMedDate not supplied

[Flow cytometry, basic principles and applications--with special reference to pediatrics].

Flow cytometry in clinical applications and research is the result of knowledge and techniques concerning cyto and fluorochemistry, laser technology, monoclonal antibodies and computer processing. Flow cytometry can assess multiple physical and biological cell properties, in a relatively short period of time. It is specially important, that specimens can concern different types of material: fresh solid tissue, embedded in paraffin, blood, bone marrow, body fluids. Under optimal conditions (standarization of method and quality control) precise and objective analysis of a large number of cells is provided. An experienced team and cooperation with clinicians and pathologists are indispensable for correct clinical interpretation of flow cytometry data. The aim of this study mainly concerns: general principles of flow cytometry operation, clinical applications--especially in paediatrics. It is also an encouragement to use this valuable and modern instrument in clinical and research work.

Adolescent↗
PubMedDate not supplied

[The effect of tobacco smoking on the human immune system].

The influence of tobacco smoking in development of respiratory, cardiovascular diseases and impairment of foetal development is well documented. Recent studies have shown adverse effects of tobacco smoke on the immune system. Particular place of impact of tobacco smoke is BALT (bronchus-associated lymphoid tissue). Tobacco smoking increases the inflammatory processes and also has an immunosuppressive effect. It is very important in relation to more frequent youth and children diseases linked with the immune system. In school education programmes to prevent tobacco smoking carried out by the Institute of Mother and Child it is also very important to include this aspect.

Adolescent↗
PubMedDate not supplied

[Analysis of DNA histograms and histopathologic assessment of necrosis after preoperative chemotherapy of osteosarcoma in children and youth].

Analysis of nucler DNA content (DNA histograms by flow cytometry) in 26 children and youth with osteosarcoma treated at the National Research Institute of Mother and Child, was carried out. The relationship between aneuploid populations, index DNA and phases of cell cycle and the clinical course, histopathologic grading and histopathologic assessment of preoperative chemotherapy effect (% of tumour necrosis) were analysed. Osteosarcomas in children with changed schedules of chemotherapy (therapy complications) in relation to the above parameters were also examined. The results show, that increased aneuploid population and index DNA (less distinct for S phase of anueptoid population) are linked with weaker chemotherapy effect. It can indicate a bigger proliferative potential in this kind of tumours - it often occurs with a more dramatic course of disease. According to the authors flow cytometry studies are helpful and complementary to histopathologic diagnosis.

Adolescent↗
PubMedDate not supplied

Multiple immune serum injections in prevention of murine "L-1210" leukemia growth.

Adult BDF1 and DBA/2J mice were inoculated i.p. with "L-1210" leukemia cells and then received i.p. control or immune sera raised in C27B1/6 and BALB/c mice. Undiluted sera were administered in single or multiple doses ranging from 0.2 to 0.4 ml/mouse on day 0., resp. 0., 2-4, and 7. In BDF1 hybrids permanent survivals (greater than 150 days) and distinct prolongation of the mean survival time (MST) after multiple injections were observed. However, normal serum derived from non-immunized C57B1/6 donors was also demonstrated to provide protection when injected three times. In DBA/2J recipients no permanent survivals were observed. In this mouse strain control sera proved ineffective. On the contrary, immune sera enabled the recipients to survive longer and this prolongation proved to be statistically significant (p less than 0.02). Partial natural immunity against "L-1210" leukemia in BDF1 hybrids must be, therefore, postulated. It is probably due to H-2-locus incompatibility versus "L-1210" cells, inherited from the C57B1/6 ancetor-line. Unknown factors which are present in normal serum seem to potientiate this natural resistance. In compatible DBA/2J mice normal serum constituents were ineffective, on the other hand, however, the effectiveness of specific immune factors directed against target cells of the tumor could be demonstrated in them

Animals↗
PubMedDate not supplied

[Study of expression of surface antigen p72 using monoclonal antibody 79IT/36 and the effect of ricin A chain-bound 79IT/36 on phytohemagglutinin-stimulated human lymphocytes].

Monoclonal antibody 791T/36 directed against surface antigen on the human osteosarcoma cell line and cross-reacting with surface antigen p72 presenting on human PHA-stimulated T-lymphoblasts was used in analysis of p72 antigen expression on human mononuclear peripheral blood cells, between 2-4 days of culture. Using FACS-IV system and fluorescence microscope, it was shown that expression of p72-antigen is dependent on PHA-stimulation and the ability of lymphocytes proliferation. The expression of p72 preceding the entry of the cells into the cell cycle. This is not significantly dependent of cell size, stage of the cycle, and RNA-transcription activity. In PHA-stimulated cultures, between 2-4 days, the number of lymphoblasts expressing enough receptors for 791T/36 monoclonal antibody is sufficient to exhibit a distinct effect of Ricin A-chain conjugated with 791T/36 over 50% inhibition of 3H-Thymidine incorporation into the cells. These observations emphasises the importance of cross-reactions in cases using immunotoxins.

Antibodies, Monoclonal↗