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Biomedical subjects

K Roszkowski

Publications and source records attributed to K Roszkowski.

At least 55 records · Page 3Linked to original sources
PubMed1988

Studies on a biological response modifying LYSAT from Propionibacterium avidum KP 40.

Propionibacterium avidum KP 40 cells were mechanically disintegrated in order to obtain the soluble immunostimulatory (antineoplastic) LYSAT. Chemiluminescence measurements of human leukocyte function yielded enhanced activation of the cells after incubation with 2.5 and 5 mg of LYSAT. As compared to non-treated controls, administration of LYSAT to BALB/c-mice (1, 2.5 and 5 mg; intraperitoneally, subcutaneously, intranasal; 7, 4 and 2 days prior to challenge) induced a significant enlargement of the spleen as well as significantly reduced sarcoma L-1 lung colonization 14 days after challenge and evidently enhanced chemiluminescence response of peritoneal macrophages.

Adjuvants, Immunologic↗
PubMed1988

Intestinal microflora of BALB/c-mice and function of local immune cells.

BALB/c-mice were treated for 7 days with oral nonabsorbable dosages of mezlocillin to achieve digestive tract decontamination. Such a procedure resulted in rapid eradication of most species of aerobic and anaerobic intestinal microflora. Various functions of peritoneal macrophages (e.g. chemiluminescence response, chemotactic motility, bactericidal and cytostatic ability) and lymphocyte proliferation were decreased in decontaminated animals as compared to non-treated controls.

Animals↗
PubMed1987

Inhibition of liver metastasis in mice by blocking hepatocyte lectins with arabinogalactan infusions and D-galactose.

According to our hypothesis, organ-specific lectins (e.g., the D-galactose-specific hepatic binding protein) play an important role in the organ location of metastatic malignant cells. The rapid clearance and uptake by the liver of tritiated alpha 1-acid-(asialo)glycoprotein from the circulation of Balb/c mice was markedly delayed after preinjection of D-galactose or arabinogalactan. The preinjection (1 h) and regular application (for 3 days after tumor cell inoculation in Balb/c mice) of the receptor blocking agents D-galactose and arabinogalactan prevented the settling of sarcoma L-1 tumor in the liver completely, but did not influence the settling in the lung. Other galactans, dextrans, and phosphate-buffered saline showed no effect. Therefore, when lectins were blocked with competitive-specific glycoconjugates, colonization was prevented.

Animals↗
PubMed1987

Antibiotic treatment, intestinal aerobic microflora and experimental sarcoma L-1 growth in Balb/c-mice.

The present paper deals with the influence of a 10 days treatment with mezlocillin, piperacillin, cefotaxime, clindamycin or gentamicin on the endogenous intestinal microflora of Balb/c-mice and on the local growth of sarcoma L-1 tumor. Clindamycin and gentamicin demonstrated no influence, whereas cefotaxime and piperacillin caused the eradication of gram-negative resp. gram-positive bacteria but these antibiotics didn't produce a growth inhibition of local L-1 sarcoma tumor. The oral or parenteral application of mezlocillin (a 3 days treatment was sufficient) eradicated the complete aerobic and anaerobic intestinal microflora. This effect was significantly correlated with an increase of the cecum weight and the inhibition of local tumor growth. Possible mechanisms of these effects are discussed.

Animals↗
PubMed1986

[Animal experiment studies on the modification of the immune system by ofloxacin].

Ofloxacin, one of the new group of quinolone antibiotics, was investigated with respect to its possible interactions with different parameters of the immune system. The study was performed in vivo on Balb/c-mice treated for seven days with different doses of the antibiotic and in vitro on human phagocytes from peripheral blood. It was found that ofloxacin does not affect cellular or humoral immune responses in mice. Moreover, human phagocytic cells exposed in vitro even to high concentrations of the drug did not show any significant changes in their function. On the other hand, it was observed that subinhibitory concentrations of ofloxacin induced an increased susceptibility of Staphylococcus aureus 511-Jena to the bactericidal activity of phagocytes.

Animals↗
PubMed1986

Effects of ciprofloxacin on the humoral and cellular immune responses in Balb/c-mice.

The effects of a 7 days chemotherapy with ciprofloxacin on the humoral and cellular immune responses in Balb/c-mice were examined. Ciprofloxacin-doses used were calculated on a body weight basis from therapeutic dosages in humans. In a dose-dependent way IgM as well as IgG responses were significantly increased. The delayed-type hypersensitivity reaction to oxazolone was not significantly changed. In in vivo as well in vitro experiments ciprofloxacin showed no modulatory activity on the concanavalin A and LPS-induced proliferative activities of mouse spleen cells.

Animals↗
PubMed1985

Small-cell lung cancer and immunochemotherapy with Propionibacterium granulosum KP 45.

Seventy-nine patients with small-cell lung cancer were treated with vincristin, methotrexate, and cyclophosphamide in inductive therapy and with methotrexate, cyclophosphamide, and procarbazine in maintenance therapy. Patients were divided at random into two groups: one group received chemotherapy alone and the second group was additionally subjected to systemic immunotherapy with Propionibacterium granulosum strain KP-45. In general, differences in the frequency of therapy response and in duration of remission could not be stated between the two groups of patients, but patients responding to chemotherapy showed a significantly longer remission time and lower complication rates. This benificial effect of chemoimmunotherapy is not related to a direct antitumor activity of the immunomodifier used, but to the lowered risk of myelosuppression and infections. Immunomodulation in combination with chemo- and/or radiotherapy can be recommended for the treatment of small-cell lung cancer.

Adult↗
PubMed1985

Antibiotics and immunomodulation: effects of cefotaxime, amikacin, mezlocillin, piperacillin and clindamycin.

The effects of 7 days' chemotherapy on the humoral and cellular parameters of the host immune system are described. In Balb/c mice the effects of cefotaxime, amikacin, mezlocillin, piperacillin and clindamycin were examined. The delayed-type hypersensitivity reaction, as well as the IgM and IgG responses, were suppressed by four of the five drugs tested: cefotaxime, amikacin, mezlocillin and piperacillin. One to two weeks after completion of chemotherapy with cefotaxime and amikacin, these parameters returned to normal values, whereas the mezlocillin- or piperacillin-modified reactions were still suppressed after 20 days. The concanavalin A and lipopolysaccharide-induced proliferative activities of mouse spleen cells were suppressed, especially by mezlocillin. The possible consequences of the immunomodulating effects of antibiotics for antimicrobial chemotherapy are discussed.

Amikacin↗
PubMed1984

Effects of cefotaxime, clindamycin, mezlocillin, and piperacillin on mouse sarcoma L-1 tumor.

For the study described in the paper, the effects of 10 days' chemotherapy with cefotaxime, clindamycin, mezlocillin, and piperacillin on local tumor growth and on spontaneous or artificial metastatic spread into the lungs were studied. For the animal tumor model Balb/c mice and the mouse sarcoma L-1 tumor were used. Chemotherapy was administered before, immediately after, or some time after the injection of tumor cells. The antibiotic dosage given to mice was calculated on a body weight basis from the doses recommended for humans. Cefotaxime and clindamycin did not influence the animal tumor model, whereas mezlocillin and piperacillin showed positive or negative effects depending on the chemotherapy schedule. In vitro none of the four antibiotics caused cytotoxic activity in cell cultures of mouse sarcoma L-1, human lung cancer E-14, or human malignant melanoma MEW.

Animals↗
PubMed1984

Rifampicin-induced suppression of antitumor immunity.

The effect of rifampicin on transplantable tumor growth and antitumor immunity was studied. The growth of transplantable lung sarcoma was significantly enhanced in mice when rifampicin was given for 7 days or longer. Also, the antitumor effects of preimmunization of the animals with killed tumor cells were abrogated by this antibiotic. In vitro studies showed that spleen cells from tumor-bearing animals expressed significantly lower specific cytotoxicity toward syngeneic tumor cells, when the animals had been previously treated with rifampicin. Also, natural killer activity in healthy animals was diminished in mice receiving the antibiotic.

Animals↗
PubMed1983

Immunochemotherapy of breast cancer with Propionibacterium granulosum.

Eighty-two patients with metastatic breast cancer were treated with adriamycin, cyclophosphamide, and 5-fluorouracil (FAC). These patients were divided by randomization into two groups. One was receiving cytostatic drugs only, while another received the identical cytostatic medication plus additional therapy consisting of IV infusions of gamma-radiation-sterilized cells of Propionibacterium granulosum strain KP-45. Metastases were localized in six different sites and/or organs. Multiple parameters for monitoring changes in metastatic lesions and laboratory tests were performed serially in all patients. The addition of P. granulosum to standard (FAC) chemotherapy of metastatic breast cancer influenced metastases in the liver. It also resulted in increased survival time within the observation period. Possible mechanisms of immunomodulating activity of P. granulosum in this disease are discussed.

Adult↗
PubMed1981

Macrophage and T lymphocyte content of tumors in mice treated with Propionibacterium.

Macrophage and T lymphocyte content of sarcoma tumors in mice treated with Propionibacterium granulosum (PG) by different routes was investigated and compared with the effectiveness of the therapy. Two different methods for tumor macrophage detection, the Fc receptor analysis and phagocytic properties, were employed. Indirect fluorescent staining of theta-antigen on lymphocytes was used to evaluate the proportion of tumor T cells. Systemic treatment with PG appeared to be effective if given soon after tumor implantation; however, this route of administration failed to affect the tumor growth if given when the tumor was already advanced. The therapeutic efficiency of intralesional PG injection was almost as effective as early systemic treatment. We obtained evidence indicating that effectiveness of PG therapy was associated with an increase of the macrophage and T cell content of tumors.

Animals↗
PubMed1981

Effect of whole-body hyperthermia on delayed cutaneous hypersensitivity to oxazolone in mice.

A murine model of delayed cutaneous hypersensitivity has been used to study an effect of microwave whole-body hyperthermia on the immune system. Marked suppression of immune responsiveness was observed in mice exposed to repeated sessions of hyperthermia. Cell proliferation in regional lymph nodes of mice sensitized with oxazolone was significantly impaired as measured by uptake of 125I-iododeoxyuridine (125IUdR). The proportion of theta-positive cells and the total number of lymph node lymphocytes remained unchanged. Inhibition of proliferation of lymph node lymphocytes in vivo was accompanied by a decrease in mitogen-induced stimulation of lymphocytes in vitro. In particular the phytohaemagglutinin (PHA) response of T cells was affected by hyperthermia. It is concluded that whole-body hyperthermia can be a potent immunosuppressive factor.

Animals↗
PubMed1981

Immunostimulation by systemic administration of Propionibacterium granulosum in patients with primary or secondary lung tumours.

The effect of a cell suspension of Propionibacterium granulosum strain KP-45 given intravenously to patients with advanced primary or secondary lung carcinoma, and simultaneously treated with cytostatic drugs, was estimated. The control group consisted of patients treated with cytostatics only. The clinical state of patients treated with the KP-45 preparation and with this immunostimulant plus cytostatics, was compared by evaluation of tumour size by X-ray examination, peripheral blood picture, and biochemical and immunological parameters. Treatment with P. granulosum KP-45 did not cause any serious adverse reaction or complication. No evident inhibitory effect on primary or secondary lung tumour development was observed. However, injections of the KP-45 preparation in cytostatics-treated patients resulted in decreased adverse effect of these drugs on the haemopoietic system and absence of bacterial infections, in contrast to patients treated with cytostatics only.

Adult↗