Biomedical subjects
Publications and source records attributed to K Porter.
The alexidine mouthrinse used in this study (CP-101) is effective in reducing plaque. There was a reduction in gingivitis after 6-months use but not at a statistically significant level. An extrinsic brown stain occurred at a significant level on the teeth of participants using the active mouthrinse. Blood and urine laboratory results indicated that alexidine can be safely used over a 6-month period.
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Fifty-seven specimens of attached gingiva have been stained for elastic fibers. The number of elastin-like fibers in the papillary layer and reticular layer of the lamina propria have been scored using a scale of 0 to 3. Using similar H & E stained sections, the amount of inflammation was also scored. The presence of elastinlike fibers in the reticular layer of lamina propria of the attached gingiva has been described. Fibers have been found in the walls of blood vessels and they have been found intermingled with the dense collagenous tissue. While an attempt was made to establish a relationship between the relative number of elastin-like fibers and patient's age or degree of inflammation, lack of sufficient number of specimens at age troups precluded this.
Human gingival explants were cultured for 1 or 2 days in a natural medium without serum. After 1 day in vitro the explants were partly encircled by epithelium which had proliferated from the cut edges of the explant and from rete ridges near the cut edge (epiboly). There was also some cell division in the cells of the spinous layer, and a thick parakeratotic layer had developed. These areas of proliferation were disclosed by the uptake of thymidine-2-C14 by the epithelial cells. Degenerative changes were also evident after 1 day in vitro. This began as hydropic degeneration in the spinous layer of the epithelium and resulted in almost total acantholysis and desquamation of the spinous layer after 2 days in vitro. The addition of serum to the medium might have promoted more epithelial proliferation and delayed the degeneration. The use of thymidine-2-C14 in the medium was an effective method of labeling the active epithelial cells.
Synchronized populations of Chinese hamster ovary (CHO) cells in confluent culture have been examined by scanning electron microscopy and their surface changes noted as the cells progress through the cycle. During G(1) it is characteristic for cells to show large numbers of microvilli, blebs, and ruffles. Except for the ruffles, these tend to diminish in prominence during S and the cells become relatively smooth as they spread thinly over the substrate. During G(2) microvilli increase in number and the cells thicken in anticipation of rounding up for mitosis. It appears that the changes observed here reflect the changing capacity of CHO cells during the cycle to respond to contact with other cells in the population, because, as noted in the succeeding paper (Rubin and Everhart), CHO cells in sparse nonconfluent cultures do not show the same wide range of changes during the cell cycle. Normal, nontransformed cells of equivalent type in confluent culture are essentially devoid of microvilli, blebs, and ruffles. The relation of these surface configurations to the internal structure of the cell is discussed.
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Three patients with primary subacute epiphyseal osteomyelitis are reviewed. All had a history of minor trauma and complained of pain and limp; none was systemically ill. Plain radiographs demonstrated a well-defined lytic lesion in the epiphysis. One case occurred in the distal tibial epiphysis from which pneumococci were isolated; this appears not to have been described previously. Patients were treated with flucloxacillin and two without operative intervention; all recovered completely. Follow-up radiographs at 2-5 years demonstrated healing of the lesions with no evidence of damage to the epiphysis or the joint. There have been 10 previously reported cases in the literature, all treated by antibiotics and curettage. Based on this experience we believe that administration of appropriate antibiotics and immobilisation of the affected limb are sufficient treatment for some children presenting with this condition.