Search PubMed⌕ Search

Biomedical subjects

K Owada

Publications and source records attributed to K Owada.

At least 55 records · Page 3Linked to original sources
PubMed1985

Association of specific tyrosine phosphorylation with stages of B-cell differentiation in human lymphoid leukemias.

In vitro protein phosphorylation in various types of human fresh lymphoid leukemic cells (C-ALL, B-CLL, HCL and PCL: B-cell lineage and T-ALL, ATL and T-CLL: T-cell lineage) were studied. In cases of B-CLL and HCL, tyrosine protein kinase (TPK) activity was at least 5-fold higher than that in other cases of B- and T-cell lineages. B-cell leukemic cells at various differentiation stages had different endogenous substrates in tyrosine phosphorylation as well as distinct TPK activity. The P-tyr-containing proteins of 68K, 59K and 56K were detected commonly in all the cases of B-cell lineage. The phosphorylated protein of 32K was present only in cases of PCL. On the other hand, in T-ALL and ATL, the major substrate in tyrosine phosphorylation was 58K. These results suggest that the characterization of in vitro tyrosine phosphorylation provides a new means not only to distinguish T- and B-lymphoid leukemia, but also to differentiate stages of lymphoid development.

B-Lymphocytes↗
PubMed1985

Urotensin II-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish.

Antiserum generated against synthetic urotensin II of the goby, Gillichthys mirabilis, was used to localize urotensin II in the caudal neurosecretory system in six species of freshwater teleosts: Cyprinus carpio, Carassius auratus, Oreochromis mossambicus, Oreochromis niloticus, Salmo gairdneri and Plecoglossus altivelis, and six species of seawater teleosts: Acanthogobius flavimanus, Pagrus major, Parapristipoma trilineatum, Trachurus japonicus, Seriola dumerili and Seriola quinqueradiata. In the carp, urotensin II-immunoreactive perikarya were classified into three groups according to their size and shape. Small cells were located in the spinal cord dorsal to the urophysis, medium-sized cells immediately anterior to the urophysis, and large cells anterior to the medium-sized cells. In each group, a small number of nonreactive cells was found. Urotensin II-immunoreactive nerve fibers extended toward the urophysis and terminated around the blood vessels. Other species of teleosts showed a similar immunoreaction to that observed in the carp. The immunoreaction of the urophysis was stronger in seawater fish than freshwater fish. Urotensin II-immunoreactive elements could not be detected in the brains of the carp, goldfish and goby.

Animals↗
PubMed1985

Immunohistochemical investigation of urotensins in the caudal spinal cord of four species of elasmobranchs and the lamprey, Lampetra japonica.

In the four species of elasmobranchs examined (Triakis scyllia, Heterodontus japonicus, Scyliorhinus torazame, Dasyatis akajei), all identifiable caudal neurosecretory cells and their corresponding neurohemal areas showed urotensin II (UII)-immunoreactivity with varied intensity. To localize urotensin I (UI) in the caudal neurosecretory system of the dogfish, Triakis scyllia, h-CRF (1-20) antiserum that cross-reacts with UI was used in place of UI antiserum. CRF/UI-immunoreactivity was demonstrated in the neurosecretory cells and neurohemal areas. A considerable number of neurons showed both UII- and CRF/UI-immunoreactivities, suggesting that UII and UI are produced in the same neurosecretory cells. However, some neurons exhibited UII-immunoreactivity, but no CRF/UI-immunoreactivity. Cells immunoreactive only to CRF antiserum were not detected. At least two populations of neurons exist in the dogfish caudal neurosecretory system: (i) cells immunoreactive for both CRF/UI and UII, and (ii) cells immunoreactive for UII. The dorsal cells of the lamprey, Lampetra japonica, did not react with either UII or CRF antiserum.

Animals↗
PubMed1984

[Observation of magnetic fields from three directions, and the moving image of the heart in a normal subject].

Three components of the magnetic field generated by the heart over the anterior aspect of the chest in a normal subject were detected using a Superconducting Quantum Interference Device (SQUID) magnetometer with a single detecting coil in a magnetically shielded room. Contour maps and color density maps of the field were prepared by computer. Movies of sequential magnetic fields of the vertical component on the anterior aspect of the chest were made from these maps. In the movies, the maximum moved from the upper central portion to right lower portion in the early stage of ventricular excitation. It moved to the central portion in the middle stage, then returned to the upper central portion in the late stage. Three-dimensional maps of instantaneous magnetic fields over the anterior portion of the chest were derived from maps of three components of the field, and from presumed heart vectors and were drawn as arrows, based on the Viot-Savart theorem. Heart vectors from these field maps corresponded well with septal forces in the early stage, left ventricular free wall force in the middle stage and the basal force in the late stage, respectively. However, large circulating vectors in the form of clockwise rotation in the central portion were also presumed. These circulating heart vectors did not correspond with the sequence of cardiac excitation as reported by Durrer et al. This discrepancy may have been caused by the fact that the vertical electric current could not be expressed from our display for presuming an electric force. Therefore, new displays and interpretations may be required for the expression of heart vectors.

Heart↗
PubMed1984

[Hypereosinophilic syndrome associated with extensive asynergy of the left ventricular wall: a case report].

This is a report of a case of the hypereosinophilic syndrome associated with extensive asynergy of the left ventricular wall. This 41-year-old man's hospital admission in March, 1982 was for congestive heart failure, with marked eosinophilia (34%). Slight depression of the ST segments and flatness of the T waves were observed in electrocardiographic leads I, II, III, a VF, and V 5-6. Digitalis and diuretics were ineffective, but supplementary prednisone therapy resulted in a decrease in eosinophilia and improvement of congestive heart failure. He was transferred to our hospital for further examination in May, 1982. There were no signs of congestive heart failure, but a third heart sound was detected on admission. Marked ST segment depression and inverted T waves were observed in the left precordial leads. Echocardiography cardiography showed extensive asynergy of the left ventricular wall, and progressive enlargement of the left atrium and left ventricle. High density area near the apex of the left ventricle suggested the presence of mural thrombi. A left endocardial biopsy showed organized thrombi sparsely invaded by eosinophils. There were no signs nor symptoms of peripheral emboli. Tapering of the dose of prednisone was attempted twice, but each time eosinophilia resulted. The patient is now free of symptoms, and is being maintained on 25 mg of prednisone daily.

Adult↗
PubMed1983

[Case of polycystic kidney associated with non-ruptured cerebral aneurysm].

Polycystic kidney is a familial disorder known to be complicated often by liver cyst, pancreas cyst and cerebral aneurysm. While numerous reports have been made on the concurrence polycystic kidney and cerebral aneurysm in foreign countries, there are only several autopsy cases documented so far in Japan. This paper describes a surgically treated case of proven non-ruptured aneurysms of bilateral middle cerebral arteries associated with polycystic kidney, and discusses the epidermology of cerebral aneurysm accompanying polycystic kidney in Japan. According to the Annual Report of Pathological Autopsy Cases in Japan, 1981, 8.4% of the cases of polycystic kidney were associated with cerebral aneurysms. The patients with polycystic kidney should be investigated for cerebral aneurysms.

Adult↗
PubMed1982

Quantitative estimation of the right ventricular overloading by thallium-201 myocardial scintigraphy.

Thallium-201 myocardial scintigraphy was performed on 55 patients with various types of right ventricular overloading. The right ventricular (RV) free wall was visualized in 39 out of the 55 patients (71%). The mean values of right ventricular systolic pressure (RVSP) and pulmonary artery mean pressure (PAMP) in the visualized cases (uptakers) were 54.6 +/- 24.1 and 30.5 +/- 15.3 mmHg, respectively. These values were significantly higher than those of the non-visualized cases (non-uptakers). There were 12 RVSP-"normotensive" uptakers and 15 PAMP-"normotensive" uptakers. The RV free wall images were classified into three types according to their morphological features. Type I was predominantly seen in cases of RV pressure overloading, type II in RV volume overloading and type III in combined ventricular overloading. RVSP in the type III group was significantly higher than that in other two groups. The radioactivity ratio in RV free well and interventricular septum (IVS), the RV/IVS uptake ratio was calculated using left anterior oblique (LAO) view images. The RV/IVS uptake ratio closely correlated with RVSP and PAMP (r = 0.88 and 0.82, respectively). In each group of RV free wall image, there were also close correlations between the RV/IVS uptake ratio and both RVSP and PAMP. Our results indicate that the RV/IVS uptake ratio can be used as a parameter for the semi-quantitative estimation of right ventricular overloading.

Adolescent↗
PubMed1982

[Comparative studies on right ventricular pressure and volume overloading by thallium-201 myocardial scintigraphy].

Thallium-201 myocardial scintigraphy was performed in 44 patients with various heart diseases including mitral stenosis (MS), atrial septal defect (ASD), primary pulmonary hypertension (PPH), and left atrial myxoma. The morphological findings of right ventricular (RV) free wall on the scintigram and RV/IVS (interventricular septum) uptake ratio of the images obtained from the left anterior oblique projection were studied in the patients with RV pressure or volume overloading. The RV free wall was visualize by scintigraphy in 13 out of 22 patients (59%) with MS, and in 15 out of 17 patients (88%) with ASD. In 5 patients with PPH or left atrial myxoma, the RV free wall was visualized in all cases. The patterns of RV free wall image were classified into three types. Type I with the smaller right ventricle than left ventricle was mainly seen in cases of MS, and type II with the dilated right ventricle was seen in cases of ASD and PPH. The RV free wall was visualized in most of the patients with MS whose RV systolic pressure (RVSP) was higher than 35 mmHg and mean pulmonary artery pressure (PAMP) was 20 mmHg or more, and with ASD whose RVSP was higher than 30 mmHg and PAMP was 10 mmHg or more. The ratio of radioactivity on the RV free wall and interventricular septum (RV/IVS uptake ratio) was calculated using 45 degree left anterior oblique view images. The RV/IVS uptake ratio ranged from 0.38 to 0.73 in the cases with MS, from 0.40 to 0.77 in the cases with ASD, and from 0.64 to 0.79 in the cases with PPH. In two cases with left atrial myxoma, the ratio was 0.50 and 0.55, respectively. The RV/IVS uptake ratio was compared to various hemodynamic parameters in right ventricular overloading. In the cases with MS, the RV/IVS uptake ratio was closely correlated with RVSP (r = 0.93: p less than 0.001), PAMP (r = 0.92; p less than 0.001), pulmonary capillary wedge pressure (PCWP) (r = 0.83: p less than 0.01), RV work index (RVWI) (r = 0.82: p less than 0.001), and pulmonary vascular resistance (PVR) (r = 0.71: p less than 0.01), respectively. In the cases with ASD, the RV/IVS uptake ratio was closely correlated with RVSP (r = 0.89: p less than 0.001), PAMP (r = 0.68: p less than 0.01), PVR (r = 0.77: p less than 0.01), and a left-to-right shunt ratio (r = 0.785: p less than 0.001). It was concluded that the analysis of thallium-201 myocardial scintigraphy is valuable as a non-invasive technique for the qualitative and quantitative estimation of RV pressure or volume over-loading.U

Adult↗