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Biomedical subjects

K Ohta

Publications and source records attributed to K Ohta.

At least 829 records · Page 46Linked to original sources
PubMed1983

Serum levels of polyamines in patients with chronic renal failure.

Serum levels of total putrescine, cadaverine, spermidine and spermine were measured in 10 normal subjects, 11 nondialyzed patients with chronic renal failure, and 25 patients undergoing maintenance hemodialysis. The measurement of the serum levels of four polyamines was done with high-performance cation-exchange chromatographic separation and fluorometric detection using o-phtalaldehyde. In normal subjects, the serum levels of putrescine and spermine were 0.24 +/- 0.09 and 0.20 +/- 0.05 nmoles/ml. In patients with chronic renal failure, the levels of polyamines were obtained in the order of putrescine, cadaverine, spermidine, and spermine: 0.51 +/- 0.15; 0.05 +/- 0.01; 0.34 +/- 0.08; and 0.05 +/- 0.04 nmoles/ml. In dialyzed patients, predialysis values of polyamines in the same order as above were: 0.88 +/- 0.31; 0.12 +/- 0.10; 0.67 +/- 0.31; and 0.09 +/- 0.08 nmoles/ml. The results show that, compared to normal subjects, the serum levels of all four polyamines are significantly elevated either in nondialyzed patients with chronic renal failure or in dialyzed patients. In dialyzed patients, the postdialysis putrescine and spermidine levels are significantly low. Serum levels of putrescine and spermidine are both significantly correlated with serum urea nitrogen and serum creatinine in a combined group of normal subjects and patients with chronic renal failure. In dialyzed patients, none of the four polyamine serum levels show correlations either with serum urea nitrogen or serum creatinine; with hematocrit, only spermine exhibits a correlationship, whereas other polyamines do not.

Blood Urea Nitrogen↗
PubMed1983

[Clinical practice of systemic hyperthermia therapy and physiological responses of the host].

Hyperthermia is a totally different modality from existing treatment modalities. Systemic hyperthermia (S-HT) is effective against advanced tumors which make resistance to conventional cancer therapies. In S-HT, it is essential and very important to manage cardio-pulmonary function in good condition. Especially, PEEP (about 7 cm H2O) is very effective to prevent lung edema. Fifty-four patients with a variety of neoplasms were subjected to S-HT, alone or in combination with chemotherapy, radiotherapy, and immunotherapy. S-HT was performed under general anesthesia by using extracorporeal circuit in corporating a heat exchanger. Usually, S-HT was given for 4-8 hours with 41.5-42.0 degrees C at 2 weeks intervals. Out of 25 evaluable cases, response was obtained in 11 cases (44%) including 2 cases of complete response. Cardio-pulmonary performance was evaluated using a flow directed pulmonary artery catheter (Swan-Ganz catheter). At treatment temperature, all patients showed hyperdynamic conditions and developed a two-fold mean increase in cardiac index. Altogether 172 treatment sessions were associated with sinus tachycardia and a reduction in diastolic pressures. Laboratory abnormalities included thrombocytopenia without sign of D.I.C., moderate hyperglycemia, mild degree of hypophosphatemia, hypolcalemia and transient elevations in liver enzymes. Serum creatinine levels were elevated in all treatment sessions without elevation of serum BUN. Serum levels of calcium and magnesium were stable. All of abnormalities and toxicities were decreased within 1 to 2 weeks after treatments. It is suggested that with carefully monitored conditions S-HT be performed safely without heart failure.

Adenocarcinoma↗
PubMed1982

Amine metabolite profile of normal and uremic urine using gas chromatography--mass spectrometry.

A method for the simultaneous analysis of phenolic amines and aliphatic amines in human urine is described. The amine metabolites in urine were extracted using Dowex 50W-X8 cationic resin, derivatized and analyzed by a gas chromatographic--mass spectrometric--computer system. The amine metabolites profile of 5 ml of urine was obtained with good gas chromatographic separation. The gas chromatographic method described here separates urinary phenolic amines, di- and polyamines and methylguanidine in a single chromatographic separation. The urinary levels of methylguanidine, putrescine, cadaverine, spermidine, p-tyramine, dopamine, and 3-methoxytyramine were quantitated by using a mass spectrometric technique. In uremic patients, only the urinary excretion of methylguanidine was increased in comparison with normal subjects, although the urinary excretion of other amines was decreased in uremic patients.

Amines↗
PubMed1982

Inhibition of prolyl hydroxylase activity and collagen biosynthesis by the anthraquinone glycoside, P-1894B, an inhibitor produced by Streptomyces albogriseolus.

P-1894B, a potent prolyl hydroxylase inhibitor produced by Streptomyces albogriseolus subsp. No. 1894, inhibited about 50% of the activity of purified chick embryo prolyl hydroxylase at a concentration of 2.2 x 10(-6) M. The inhibition was noncompetitive with respect to (Pro-Pro-Gly)5 with a Ki of 1.8 x 10(-6)M. When excess amounts of ferrous ions or ascorbate were added to the reaction mixture, the inhibition was slightly reversed. P-1894B at a dose of 0.15 mg/kg reduced the hydroxylation of peptidyl proline and caused a significant inhibition of collagen biosynthesis in the uterus of the immature rat stimulated by the administration of estradiol-17 beta.

Animals↗
PubMed1982

Gas chromatographic-mass spectrometric analysis of amine metabolites in urine.

An analytical method for the separation and identification of amine metabolites in human urine has been developed using a gas chromatograph-mass spectrometer-computer system. The sample preparation consists of organic solvent extraction, adsorption on a cation-exchange resin, evaporation, and acylation. N-3-Hydroxypropyl-1, 4-diaminobutane was identified for the first time in urine. Methylguanidine, guanidine, putrescine, cadaverine, p-tyramine, 3-methoxytyramine, spermidine, and spermine were detected in normal urine with good gas chromatographic separation.

Amines↗
PubMed1982

Dietary fat affects immune response, production of antiviral factors, and immune complex disease in NZB/NZW mice.

Autoimmune-prone (NZB x NZW)F1 (B/W) mice fed three nearly isocaloric diets with varied fat content showed a marked difference in their spontaneous development of immune complex disease and their immune response. Those animals received the diets high in either unsaturated or saturated fats had more severe immune complex nephritis and died earlier than mice on the low-fat diet. Endogenous production of the mouse xenotropic virus was unaffected by dietary fats, but the serum lipoproteins associated with antiviral activity were increased to levels as high as 1:600,000 in the B/W mice on the high-fat diets. These lipoproteins may be partially responsible for the decreased mitogenic response of spleen cells from mice fed the two high-fat diets. The mice receiving a diet high in saturated fats produced substantially higher titers of natural thymocytotoxic autoantibody, an IgM class of antibody, than did the mice maintained either on the high-unsaturated-fat or low-fat diet. In contrast, the mice receiving the diet high in unsaturated fats made significantly greater levels of antibodies to double-stranded DNA, an IgG, than did the mice kept on the two other diets. These results suggest that the type of fat in the diet could affect the serum level of different immunoglobulin classes. The data provide further evidence that the amount of dietary lipids alone can influence cellular and humoral immune responses and the spontaneous development of immune complex disease.

Animals↗