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Biomedical subjects

K Nowak

Publications and source records attributed to K Nowak.

At least 37 records · Page 2Linked to original sources

[The assessment of hepatic enzymes' levels in children treated for leukemia and non-Hodgkin's lymphomas].

The purpose of this study was to determine the frequent of serum aminotransferase elevation in children with leukemias and non-Hodgkin's lymphomas and define the cause of this pathology. In the serum of 43 children the bilirubin concentration, activities of aspartic aminotransferase (AspAT), alanine aminotransferase (AlAT) and gammaglutamylotranspeptidase (GGTP) were measured before treatment, during and after intensive chemotherapy. 43 patients 8 (65%) had bilirubin concentration above 1.2 mg/dl and/or aminotransferase activities above 100 U/l. The most possible causes of the liver damage in the patients were: hepatotoxicity of chemotherapy, virus or bacterial infections and leukemic or lymphomatous involvement of the liver.

Adolescent↗

[Cytoprotective effect of amifostine in children during induction therapy according to BFM-83: report on cases].

Amifostine is the agent of proved cytoprotective activity against alkylating drugs and rubidomycine. Its protective effect against other cytotoxic drugs is doubtful. BFM-83 induction therapy for ANLL (ARA-C + RUB + VP-16) which is applied to children with acute non-lymphoblastic leukemia (ANLL) commonly contributes to severe adverse reactions. We administered amifostine to three children: 2 boys with ANLL (7 and 11 yrs) and 1 girl with MDS (3 yrs) during etoposide and rubidomycine induction therapy in order to decrease chemotherapy-related adverse reactions. Doses of amifostine were 740 mg/m2, 910 mg/m2 and 910 mg/m2 respectively. Efficacy of the therapy was evaluated on the base of blast decline in the bone marrow, efficacy of the cytoprotection by myelo and nephrotoxicity symptoms analysis. Chemotherapy-related adverse effects in the children protected by amifostine were less severe and observed by the shorter periods as compared with the historical control group of 20 patients treated according to BFM-83 without cytoprotection. These cases show the potential beneficial effect of amifostine during BFM-83 induction therapy for ANLL. The further randomised clinical study of the proposed cytoprotection should be performed to establish its value.

Amifostine↗

[The use of hematopoietic growth factors G-CSF/GM-CSF in the treatment of neutropenia in children with acute lymphoblastic leukemia and non-Hodgkin's lymphomas].

Maximal intensification of antineoplastic therapy is currently a predominant trend in the treatment regimens for acute leukemias and lymphomas. However, by such approach myelosuppression and counteracting its sequelae become paramount problems. Hematopoietic growth factors G-CSF/GM-CSF play a great role in this aspect of the therapy. Effects of 35 courses of G-CSF/GM-CSF were evaluated in 19 children with ALL and NHL and compared with 21 episodes of neutropenia in 15 historical controls. In the treatment group time of neutropenia was approx. 3 times shorter as compared with a control group. Fever accompanying neutropenia occurred less frequently and lasted shorter in the treatment group. Also, symptoms of infection subsided faster. Subjective life quality was better in children receiving growth factors.

Adolescent↗

[Central venous lines in children with cancers].

Authors analyzed clinical aspects of central venous lines in children with cancer. In 25 patients central venous catheters with subcutaneous ports and in 34 patients lines with external ending were inserted. Catheters were left in place respectively 62-836 days and 4-365 days. During that time 10 catheters were removed due to occlusion, leakage, local infection or sepsis. The causes of these complications were analyzed in discussion.

Adolescent↗

[The case of neurofibromatosis type ! in a 5-year-old boy].

In the work we present the case of a 5-year-old boy who was admitted to the clinic because of the tumour in mediastinum. According to the results of the investigation the diagnosis of Recklinghausen disease was made. The tumour was situated in the region of pharynx, neck and mediastinum. The diagnosis was based on the characteristic features in the physical examination (café-au-laît spots, freckles in armpits and grains) and accessory investigations (x-ray, CT, NMR and histopathology). In the boy's family this disease was not noticed, although in his mother we observed café-au-laît spots on the skin of abdomen. Despite the fact that the diagnosed disease is known as a mild one (from the histopathological point of view) in the case of our patient it turned out to be much more clinically malignant because of the localization of the tumour. This situation forced us to the application of much more aggressive treatment (chemotherapy, operation).

Cafe-au-Lait Spots↗

[Diagnostic problems in coexistence of paranasal sinusitis with cerebral tumor].

Differential diagnosis between the intracranial complications of paranasal sinusitis and cerebral tumor belongs to very difficult tasks in otolaryngology and neurosurgery. Complications of sinusitis, such as intracranial abscess formation, are uncommon and often clinically unremarkable. The arising difficulties are even greater in inflammatory intracranial complications of sinusitis, giving symptoms similar to those of cerebral tumors. The illustrative case report was presented.

Aged↗

HER-2/Neu expression as a progression marker in pancreatic intraepithelial neoplasia.

Pancreatic intraepithelial neoplasia is only partially defined. Any attempt to establish the diagnostic criteria of early pancreatic carcinoma has been unsuccessful so far. In the present study we investigate expression of HER-2/neu in hyperplastic pancreatic duct epithelium. Material included resected pancreatic tissue obtained from 13 patients with pancreatic carcinoma, 11 with chronic pancreatitis, and 11 patients operated on for other reasons (gastric cancer, carcinoma of papilla Vateri). Hyperplasia of pancreatic duct epithelium was scored as: 1. flat mucosal hyperplasia FH, 2. papillary hyperplasia PH, 3. atypical papillary hyperplasia APH, 4. carcinoma in situ CIS. Immunohistochemical expression of HER-2/neu was studied with the biotin-streptavidin method. Results were scored as: 1+ barely perceptible light membranous rimming, 2+ light to moderate rimming, 3+ moderate to strong rimming. Expression of HER-2/neu paralleled with the hyperplasia grading, in most cases being negative in normal duct epithelium, weak in flat hyperplasia, and moderate to strong in atypical papillary hyperplasia and carcinoma in situ. In conclusion, HER-2/neu expression could be used as an additional marker of hyperplasia, dysplasia and atypia of pancreatic ductal and ductular epithelium in the process of pancreatic epithelial neoplasia. This could be especially useful in the cytological diagnosis of pancreatic intraepithelial neoplasia.

Adult↗

A topographic analysis of atrophic gastritis and stomach cancer risk. A clinicoepidemiological study in Poland.

The purpose of this cross-sectional study was to assess the relation between intestinal and diffuse stomach cancer at its various locations with topography of atrophic gastritis. The study population consisted of 3435 patients who reported over the period of 1991-1994 for the first time to gastroenterological outpatient clinics of 7 university medical centers in Poland. Among these subjects there were 131 histologically proved consecutive cases of gastric carcinoma. The reference group consisted of 1540 patients among whom endoscopic examination did not reveal peptic ulcers, polyps, deformations of antrum or bulbus duodenum and mucosa erosions. Gastroscopy on gastric cancer patients and the reference group was performed and biopsy specimens were obtained from the tumour and from the antrum and stomach corpus distant from the tumour. Among the gastric cancer cases there was a higher prevalence of atrophic gastritis in the intestinal than in the diffuse type. The highest prevalence of atrophic gastritis irrespective of its degree and stomach area was observed in the tumour-area of intestinal cancer located distally (78.9%), and the lowest in the tumour-free area in diffuse proximal cancer (18.5%). Prevalence of atrophic pangastritis (atrophic gastritis present both in the corpus and antrum) was also highest in intestinal distal cancer (69.2%) and lowest in diffuse proximal cancer (21.7%). The age-adjusted correlation coefficients between gastritis score in the tumour-area and tumour-free area were highly significant. It was shown that only atrophic pangastritis was significantly associated with gastric cancer irrespective of its histology and location (OR = 3.8, 95% CI:2.4-6.0), however, it was much stronger related to the intestinal gastric cancer (OR = 5.9, 95% CI: 3.1-11.0), than to the diffuse carcinoma (OR = 2.2, 95% CI: 1.1-4.3).

Adult↗

[Controlled clinical studies for topographic analysis of atrophic gastritis and stomach cancer].

The purpose of this cross-sectional study was to assess the relation between intestinal and diffuse stomach cancer at its various locations with topography of atrophic gastritis. The study population consisted of 3435 patients who reported over the period of 1991-94 for the first time to gastroenterological outpatient clinics of 7 university medical centers in Poland. Among these subjects there were 131 histologically proved consecutive cases of gastric carcinoma. The reference group consisted of 1540 patients among whom endoscopic examination did not reveal peptic ulcers, polyps, deformations of antrum or bulbus duodenum and mucosa erosions. Among the gastric cancer cases there was a higher prevalence of atrophic gastritis in the intestinal than in the diffuse type. The highest prevalence of atrophic gastritis irrespective of its degree and stomach area was observed in the tumour-area of intestinal cancer located distally (78.9%), and the lowest in the tumour-free area in diffuse proximal cancer (18.5%). Prevalence of atrophic pangastritis (atrophic gastritis present both in the corpus and antrum) was also highest in intestinal distal cancer (69.2%) and lowest in diffuse proximal cancer (21.7%). For other types of cancer the prevalence rates of atrophic pangastritis was 50.0% in intestinal proximal cancer and 37.5% in diffuse distal carcinoma. The age-adjusted correlation coefficients between gastritis score in the tumour-area and tumour-free area were highly significant. It was shown that only atrophic pangastritis was significantly associated with gastric cancer irrespective of its histology and location (OR = 3.8, 95% CI:2.4-6.0), however, it was much stronger related to the intestinal gastric cancer panga (OR = 5.9, 95% CI: 3.1-11.0), than to the diffuse carcinoma (OR = 2.2, 95% CI: 1.1-4.3).

Adult↗

[The distribution of metastases to cervical lymph nodes of laryngeal and pharyngeal neoplasms. Comparison of clinical evaluations and assessment of surgical preparation].

The purpose of the study was a comparison of clinical and pathological assessment of cervical lymph nodes metastases in advanced carcinoma of larynx and the hypopharynx. The outspread, size and number of the nodes metastases to the particular regions of the neck was taken into analysis. The differences and occurrence in clinical and pathological assessment was pointed out.

Humans↗

[The Polish Medical Association, Londyn].

The Polish Medical Association, founded in 1944 in London and celebrating its Golden Jubilee in 1944, is a unique organisation of doctors brought together by the shared experience of serving in the Polish armed forces on the fronts of the II World War. Preparing themselves for the return to a free Poland and later facing the realities of the emigré existence, they formed an association based on an identity with patriotic ideals and espousing a loyalty to the national cause, solidarity with the aspirations and goals of the expatriate community and commitment to the integrity in the performance of their professional duties. It was created against a background of the already existing Polish Medical Faculty at the University of Edinburgh and supported by strong lobbying on the part of the Polish Government and the favourable political climate. The need for a professional body of Polish doctors became evident. During the first few years the Association, originally called the Polish Medical Association in the British Empire, numbered over 700 doctors and achieved notable succeses for its members. The most significant were the endeavours which contributed to the inclusion of the so-called Clause 5 of the Polish Resettlement Act 1947, which in practice allowed Polish doctors serving in the Polish forces under British command to obtain full medical registration. Almost 800 doctors benefitted from this legislature and by coincidence, with the introduction of the National Health Service, they constituted the single most significant injection of non-British doctors into the British health care system. Throughout the years the Association, which originally comprised doctors with an Army background acquired more and more civilian members. The second generation followed, smaller waves of post-war emigration brought in new members from Poland. With time the administrative structure of the Association--initially comprising ten divisions in the UK and overseas--has changed; the second generation--those already born and educated in the UK--joined the professional ranks, and later achieved the highest positions. The return of democracy to Poland, so eagerly awaited, has brought a new dimension to the Association's activities. Its original character, however--identity with the national ideals of dignity, honour, integrity--naturally, remains unchanged and deeply ingrained in the collective and individual consciousness of this unique group. The Association maintains active contacts with Poland's leading medical institutions and academic establishments and is the initiator of the ideal of integrated effort of the Polish emigré medical milieu and medicine in Poland--the ultimate goal is the long-term commitment to the health of the Polish nation.

History, 20th Century↗

Computer-aided analysis of the nuclear area in proliferative lesions of the colonic and bronchial epithelium.

The aim of this study was to establish the diagnostical value of nuclear area in proliferative lesions of colonic and bronchial epithelium. The study included 11 adenomas and 9 adenocarcinomas of colon, 11 control samples of normal colonic epithelium as well as 5 cases of bronchial squamous cell metaplasia, 25 squamous cell carcinomas, 9 small cell carcinomas and 3 adenocarcinomas of bronchi and 13 control samples of normal bronchial epithelium. All tissue samples were formalin fixed, paraffin embedded and silver stained. Morphometrical analysis was performed using optical Axiophot microscope with immersion, B/W CCD camera connected to frame grabber card, computer PC AT 386 and morphometrical software Vist and Morpho. Nuclear area was measured semiautomatically. Within each case 100 nuclei were analyzed. Statistically significant differences between carcinoma and adenoma or normal epithelium were observed in colon. In bronchi nuclear area was significantly higher in squamous cell carcinoma and adenocarcinoma if compared with other lesions including small cell carcinoma or bronchial metaplasia and normal epithelium.

Adenoma↗

Morphometric measurements of the nuclear area in proliferative thyroid lesions.

The aim of this study was to establish the diagnostic value of nuclear area in proliferative lesions of thyroid gland. This study included 98 histological preparations of thyroid and 57 cytological preparations (touch imprints). Morphometric analysis was performed using light microscopy, B/W CCD camera connected to the frame grabber card, computer PC AT 386/3 and morphometric software Vist and Morpho. Nuclear area was measured semiautomatically. Our study indicated that the nuclear area was significantly higher in cytological preparations and that size of nuclei seems to depend on the type of neoplasm and to a lesser extent on whether a given tumor was malignant or benign.

Adenoma↗

[Clinical importance of granulocyte-specific antibodies].

Clinical and laboratory data of 184 patients with immune neutropenia were evaluated. They suffered from autoimmune neutropenia (n = 165), alloimmune neonatal neutropenia (n = 18) and from transfusion-associated lung injury (n = 1). Autoimmune neutropenia was predominantly found in patients below 3 years. Patients were usually affected by benign bacterial infections. The peripheral blood count showed normal or diminished leukocyte counts with median absolute neutrophil counts of 285 cells/microliters. Bone marrow examination revealed in 60% of the cases a hypercellular marrow with a shift to the left. In 36% the bone marrow was normal and in 4% a hypocellular marrow was found. Spontaneous remission occurred in all newborns and, so far, in 4 patients with autoimmune neutropenia. Symptomatic treatment of the infections was sufficient in most of the patients.

Adolescent↗

Autoimmune neutropenia: clinical and laboratory studies in 143 patients.

Clinical and laboratory data of 143 patients with primary or secondary autoimmune neutropenia (AIN) were evaluated. Primary AIN was found predominantly in children below 3 years, whereas secondary AIN was more frequent in patients 40-60 years of age. Female patients with primary AIN were slightly more prevalent (54%) than male patients (46%). The peripheral blood count showed normal or diminished leukocyte counts with median absolute neutrophil counts of 250 cells/microliters. In 38% of the patients neutropenia was accompanied by monocytosis. Bone marrow examination revealed in 95% a normo- or hypercellular marrow with a marked reduction of mature neutrophils in 56% of the specimens. Twenty-three percent of the sera showed specificity for the NA1 antigen. Patients were usually affected by benign bacterial infections of the skin and of the upper respiratory tract, as well as by recurrent otitis media. Infections were treated symptomatically, and only six patients required continuous administration of antibiotics. Remission of neutropenia during treatment occurred in three of six patients treated with intravenous immunoglobulin G and in three of four patients who received steroid therapy. Except for one patient neutropenia relapsed after discontinuation of therapy. During a follow-up of 6-36 months, spontaneous remission has been observed in four patients.

Antibodies, Anti-Idiotypic↗

Immunohistology of gastrointestinal carcinoid tumors.

DAKO quick staining kits were used to immunostainings for Epithelial Membrane Antigen (EMA) Neuron Specific Enolase (NSE) and S-100 protein (S-100) in 18 carcinoid tumors of the gastrointestinal tract classified according to Soga and Tazawa. EMA was always absent in carcinoid tumors, being at the same time present within glandular epithelium. 88% of cases showed positive immunostaining for NSE. S-100 immunostaining showed immunopositive stellate cells present within the tumor especially within type A carcinoids. In addition in one carcinoid tumor thick, strongly S-100 positive bundles were noticed at the periphery of nests of tumor cells. Combined immunohistochemical and ultrastructural studies are needed to elucidate interrelation of neoplastic and neural elements within carcinoid tumorsa.

Adolescent↗