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Biomedical subjects

K Norrby

Publications and source records attributed to K Norrby.

At least 109 records · Page 6Linked to original sources
PubMed1978

B- and T-lymphocytes in toxic diffuse goitre.

Operation specimens of thyroid tissue from patients with Graves' disease were digested, with collagenase, down to a cell suspension. The lymphocytes in the suspension were concentrated by density-gradient sedimentation and the proportions of B and T lymphocytes were determined. Six patients with Graves' disease were studied. The proportions of B and T lymphocytes in the thyroid glands were similar to the proportions of B and T lymphocytes in the venous blood from healthy subjects.

Adult↗
PubMed1977

Different proliferative responsiveness of fibroblasts and mesothelial cells in the rat mesentery following administration of compound 48/80 at various hours of the day.

The proliferative responsiveness of fibrolasts and mesothelial cells in the mesenterial membrane of normal rats was studied quantitatively after a single i.p. injection of the mast-cell activating and histamine-releasing drug Compound 48/80. To make some allowance for a possible chronobiologic effect of the circadian type on the induced proliferation, the drug was given at 1 a.m., 9 a.m., or 5 p.m., and the animals were examined 16, 24, and 32 h later. The proliferation was estimated by cytophotometric Feulgen DNA measurements in individual fibroblast and mesothelial cell nuclei, and by mitotic frequency counting. The main result was that a larger fraction of fibroblasts than of mesothelial cells was stimulated to proliferation, regardless of the hour of treatment with Compound 48/80. It was further demonstrated that in control animals the fraction of cells of either fibroblastic or mesothelial type present in the S cum G2 cell-cycle phases varied markedly at different hours of the day. Quantitative differences appeared in the induced proliferation with regard to the hour of treatment. The most vigorous proliferative response appeared after administration of the drug at 9 a.m. The fraction of cells in the S cum G2 cell-cycle phases was then increased at 16 h and the fraction of dividing cells at 24 h after treatment, illustrating the promptness of the induced proliferative reaction.

Animals↗
PubMed1977

Induction of proliferation in dense non-starved 3T3 cells by Ca++ ionophore A23187.

The proliferogenic effect of the Ca++ ionophore A23187 was tested in dense non-starved 3T3 cells. Whereas continuous exposure during 48 h of cells to the ionophore at concentrations is larger than or equal to 0.4 muM cytotoxic, a short exposure for 30 s up to 4 min at 0.2 muM was proliferogenic. It was also found that such short exposures to the ionophore caused a transient increase in the intracellular level of cyclic GMP and a roughly simultaneously appearing decrease in the intracellular level of cyclic AMP.

Animals↗
PubMed1976

Mast cell activation and tissue cell proliferation.

The effect of mast cell activation and degranulation on the proliferation in the intact mesentery was studied in Sprague-Dawley rats. Mast cell activation was achieved by a single intraperitoneal injection of Compound 48/80. The proliferation was studied using three independent methods for estimation of cell production and DNA synthesis: 1. the mitotic index, 2. the relative number of cells having a DNA content in the S and G2 regions, by Feulgen photometric measurement in individual cells, and 3. the specific DNA activity, employing a method which combines a liquid scintillation technique after an intravenous injection of 3H-thymidine and Feulgen photometric determination of the DNA content per membrane preparation. It was found that the proliferation of the normal mesenchymal cells adjacent to the activated and degranulated mast cells in the mesentery was significantly increased within 24 and 32 h, the maximum increase being more than 20-fold compared to untreated controls. The results suggest that the common type of mast cell may have a pathophysiological function related to stimulation of local cell proliferation.

Animals↗
PubMed1976

A tissue model for the study of cell proliferation parameters in vivo.

An intact tissue model which can be used for detailed microscopic studies, quantitative cytochemical analysis and biochemical analysis has been explored, using a number of cell proliferation parameters. The preparations consist of mesenterial "windows" from rats dispersed on object slides. A technique for determining, in one and the same preparation, DNA content and mitotic activity of individual, selected cell types, and DNA synthesis in terms of incorporation of tritiated thymidine into DNA is described.

Animals↗
PubMed1975

Hypercalcaemia in chronic lymphatic leukaemia.

Hypercalcaemia developed in a patient with chronic lymphatic leukaemia. The hypercalcaemia did not respond to conventional treatment. At bone biopsy osteoclastic resorption was found (a photomicrograph is presented). 7 cases from the literature of hypercalcaemia and chronic lymphatic leukaemia are briefly reviewed. Possible mechanisms are discussed and it is suggested that osteoclast-stimulating factors may be of importance in the development of the hypercalcaemia.

Aged↗