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Biomedical subjects

K Nishiya

Publications and source records attributed to K Nishiya.

85 records · Page 5Linked to original sources
PubMed1980

Iron binding proteins in selected human peripheral blood cell sets: immunofluorescence.

Selected populations of human peripheral blood T lymphocytes, B lymphocytes, monocytes and polymorphonuclear cells were examined for their intracellular content of lactoferrin transferrin and ferritin by an indirect immunofluorescence technique. Lactoferrin was found in polymorphs but not in lymphoid cells. Two different lactoferrin staining patterns were observed which we designated 'perinuclear', characterized by a ring of positive material round the nucleus, and cytoplasmic, in which most positive material was distributed in the cytoplasm. The former staining pattern was found in high density polymorphs the latter was associated with low density polymorphs occasionally found contaminating the peripheral blood mononuclear cell suspension. After incubation in vitro, the perinuclear pattern changed to cytoplasmic staining. Transferrin was found in T cells and polymorphs. In contrast, only a few transferrin containing cells were detected in the B cell and monocyte fractions. Following overnight incubation, a halo of positive material was found surrounding T cells stained for transferrin, suggesting that T cells released transferrin during incubation. Ferritin was also found in T cells and adherent cells. Following latex particle ingestion, the intensity of ferritin staining was markedly increased. Only a small proportion of lymphoid cells and monocytes stained for transferrin or ferritin in the total peripheral blood mononuclear cell suspension. The results indicate, therefore, that in response to manipulation procedures used routinely for the selection of human peripheral blood lymphoid cells, detectable amounts of transferrin and ferritin are present in T but not B cells. The fact that T cells are equipped with proteins known to participate in binding and storage of iron may constitute, at least in part, the basis for the contribution of these cells to the regulation of other major biological systems.

Cell Separation↗
PubMed1976

Scanning electron microscopy of myeloma cells.

The surface structure of myeloma cells was examined by scanning electron microscopy. The cells were collected from the pleural effusion of a multiple myeloma patient and purified by Conray-Ficoll gradient sedimentation. The cell size ranged from 8 mu to 12 mu in diameter and the microvilli were from 0.8 mu to 1.2 mu in length. The surfaces of the majority of the observed myeloma cells were more villous than lymphocytes.

Aged↗
PubMedDate not supplied

Elevation of serum ferritin levels as a marker for active systemic lupus erythematosus.

OBJECTIVE: To determine the clinical relevance of serum levels of ferritin in patients with systemic lupus erythematosus (SLE) vs. controls, we assessed the correlations between such levels and clinical disease activity, anti-DNA antibody titer, and serum levels of complement. METHODS: We evaluated 36 patients (3 males and 33 females) with SLE, including 21 patients with active disease. A total of 52 patients (3 males and 49 females) with rheumatoid arthritis (RA) served as controls. In a further study for reproducibility, 15 SLE and 21 RA patients were examined. Serum ferritin levels were measured by a 2-site radioimmunometric assay. Serum levels of C-reactive protein (CRP) were measured semiquantitatively by immunoprecipitation or quantitatively by laser immunonephelometry. Anti-DNA antibody was measured by the Farr assay. CH50 was measured by the hemolytic activity method. RESULTS: The SLE patients exhibited higher serum levels of ferritin and lower serum levels of CRP than the RA patients. Serum levels of ferritin at the active stage of SLE exceeded those at the inactive stage. The levels of serum ferritin in SLE were positively correlated with the anti-DNA antibody titer and negatively correlated with CH50 values. CONCLUSION: Serum levels of ferritin appear to provide a useful marker of disease activity in SLE patients.

Adolescent↗