Biomedical subjects

K Nishio

Publications and source records attributed to K Nishio.

At least 217 records · Page 12Linked to original sources

PubMed1989

Isolation of a new human fetal liver cytochrome P450 cDNA clone: evidence for expression of a limited number of forms of cytochrome P450 in human fetal livers.

From a human fetal liver cDNA library, a new cDNA clone (lambda HFL10) was isolated using an antiserum to P450 HFLa, which has been isolated from livers of human fetuses. Cytochrome P450 cDNAs, namely lambda hPA6, lamda hP2-1, and lambda hPD4 which were highly homologous to cDNA clones, pHY13, Hp1-1, and phP450j, respectively, were also isolated from the cDNA library of human adult livers. Using these cDNA clones as probes together with Lambda HFL10, Northern blot analysis was conducted to determine whether all of these cytochromes were expressed in human fetal livers. The results clearly showed that only P450 HFL10 mRNA was detected in human fetal livers. This result supports the allegation that there is a much more limited number of forms of cytochrome P450 in human fetal livers than in adult livers.

Amino Acid Sequence↗

PubMed1989

Molecular cloning and sequence analysis of cDNA containing the entire coding region for human fetal liver cytochrome P-450.

From a human fetal liver cDNA library, a cDNA clone (lambda HFL33) containing the entire coding region for a form of cytochrome P-450 related to P-450 HFLa was obtained. The clone was 1,971 bp long and had an open reading frame of 1,509 nucleotides coding for a 503 amino acid polypeptide. The nucleotide and the deduced amino acid sequences of lambda HFL33 were very similar to but clearly distinct from those of NF25 and HLp cDNAs, which code for forms of cytochrome P-450 in adult human liver. The deduced N-terminal amino acid sequence of the HFL33 protein was identical to that of P-450 HFLa.

Amino Acid Sequence↗

PubMed1989

[Chewing pattern in posterior crossbite. Classification of chewing pattern in the frontal plane].

Occlusion is one of the most important factors to affect chewing movement. In this study, chewing movements of subjects who have posterior crossbite were recorded to analyze the relationship between chewing pattern and crossbite occlusion. Chewing movements of 22 subjects of posterior crossbite and 5 normals were recorded with Sirognathograph Analysing System II. Subjects were to chew soft gum; on each specified side, both at the posterior crossbite region and at another resion of the side. On the other hand, normal subjects were to chew only on each side. Chewing patterns were classified into several specific ones examined with chisquare test concerning the relationship between chewing pattern and occlusion. From the results we obtained following conclusion. 1. Frequency of normal chewing pattern, posterior crossbite group is lower than normal group. 2. Among the posterior crossbite group, specific patterns are found with highly frequency such as 'Concave Type', 'Crossover I Type', 'Crossover II Type' and 'Reverse Type'. 3. By segmental chewing at the posterior crossbite region, 'Normal Type' and 'Concave Type' are lower and 'Crossover I Type', 'Crossover II Type' and 'Reverse Type' are higher in frequency in comparison with posterior crossbite side voluntary chewing. 4. By segmental chewing at the posterior crossbite resion, 'Normal Type' and 'Concave Type' are lower and 'Crossover I Type', 'Crossover II Type' and 'Reverse Type' are higher in frequency in comparison with segmental chewing at non-crossbite resion.

Humans↗

PubMed1989

[Single left coronary artery with a fistula to the right ventricle: report of a case with successful closure].

A 4-year-old boy of single left coronary artery associated with a fistula to the right ventricle was reported. Cardiac catheterization and selective coronary angiography revealed single left coronary artery with a fistula to the right ventricle. At operation, a single coronary artery arising from the left aortic sinus was present. A dilated and tortuous left circumflex branch passed to the right and anterior . This vessel terminated into the outflow tract of the right ventricle. The dilated vessel was doubly ligated at just distal portion of a acute marginal branch. The fistula to the right ventricle was directly sutured from arteriotomy. Then three small branches from the anterior descending artery terminating into the terminal portion of the dilated vessel were found and these were ligated respectively. After repair of arteriotomy, the remainder of the dilated and tortuous vessel was wrapped with a Dacron mesh for reinforcement. Postoperative course was uneventful and there were no complications.

Arteries↗

PubMed1989

[Surgical experience with an infant with discrete subaortic stenosis successfully treated by aortoventriculoplasty].

A 3-year-old female with discrete subaortic stenosis is presented. Angiocardiographic studies showed a long segmental narrowing below the aortic valve. Although membranectomy and myectomy were selected as a surgical treatment, a longitudinal aortotomy was performed in order to extend the operation to ventriculoseptoplasty. During the operation, the right coronary cusp was seriously damaged and therefore aortoventriculoplasty was applied. The aortic valve was replaced with a 21 mm St. Jude Medical mechanical prosthesis. The left ventricular outflow tract was adequately enlarged, and postoperative course was uneventful.

Aorta↗

PubMed1989

[Vascular ring associated with severe ventricular septal defect--report of a case with simultaneously surgical treatment].

An 1 year and 4 months ill infant with vascular ring and VSD is presented. She had serious pulmonary hypertension and dysphagia because of vascular ring consisting of right aortic arch with left ligamentum arteriosum. Division of the ligamentum arteriosum and simultaneous patch closure of large VSD were performed successfully. Postoperative course was uneventful and she recovered from her body weight favorably.

Aorta, Thoracic↗

PubMed1988