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Biomedical subjects

K Nishimura

Publications and source records attributed to K Nishimura.

At least 757 records · Page 42Linked to original sources
PubMed1990

[A case of maxillary protrusion].

Achievement of best balance and facial harmony is the main goal of orthodontic treatment, and it is especially important to retract the lip in case of maxillary protrusion. A 11-year and 7-months-old girl was first seen with chief complaints of maxillary protrusion. We extracted 4/4 4/4, treated the patients with the Vari-Simplex-Discipline for 43 months, and evaluated changes in facial profiles. We found, that excessive lip protrusion was eliminated and good interdigitation obtained. In addition to, angle ANB and overjet were corrected to ideal conditions.

Cephalometry↗
PubMed1990

[A case of the Hallermann-Streiff syndrome].

The Hallermann-Streiff Syndrome has been characterized and established according to 7 positive and 5 negative signs, which were described by François. We encountered an 11 year, 5 months old boy who had 7 positive symptoms of this syndrome in our clinic. In this study, we reported on this typical patient focusing on his dental view. 1) Prolonged retention of the primary teeth which involved microdontia were noted. Congenitally missing teeth were also seen. 2) The occlusal relationship indicated open bite, and also the mandibular function was impaired. 3) The measurements of the length and width of the dental arch were smaller than that of a normal subject, and the dental arch of the maxilla was V-shaped. 4) According to X-ray cephalometric analysis, (a) the dental calculus and the alveolar bone absorption were very evident. (b) abnormal morphologic of the glenoid fossa, mandibulars condyle and the neck of mandibula were seen. These conditions were very evident on the left side. 5) The growth obstade of the maxilla and mandibla and the left shift of the mandible were found. 6) According to histological study, enamel hypoplasia was noted.

Child↗
PubMed1990

[Effect of extracellular matrix and serum components on cellular adhesion and growth in vitro and in vivo].

The recent cell-biological research have been reported the usefulness of extracellular matrix, such as collagen and fibronectin, in cell culture techniques and in vitro experiments. These matrix began to applied in medical treatments and brought many success. Fibroblast and osteoblast have characteristics to promote stabilization of implant materials in vivo. These cells revealed the additional effects to control mending and healing of surgical invasion. The role of extracellular matrix and serum components for cellular adhesion and growth have been discussed extensively. The recent studies reported that collagen and other materials promoted the cellular behavior. The author discussed the effect of extracellular matrix and serum components or the established cell lines on cellular adhesion and growth, which were performed in vitro and in vivo. The following cell lines were employed in this study, fibroblastic cell lines from mouse (3T12-3) and human (SF-TY), osteogenic cell line from mouse (MC3T3-E1) and osteogenic cell lines from human (SAOS-2, NY), osteoblastic cell line (HuO-3N1) and rabbit cell line. Different types of collagen were used as the coating material on glass surface by the method of collagen-gel culture method. Fetal calf serum, bovine serum albumin, fibronectin and proteoglycan were also employed in this study. After the cultivation, adherent cells were stained by Gimsa's method and counted microscopically. In cellular-adhesion test, the most cell number was found on the collagen type I coated glass surface to compare with the other materials, such as fetal calf serum, bovine serum albumin, proteoglycan, employed in all the tested cell lines. Growth of the defined cell-number on glass surface was compared among used materials, and all the osteogenic cell lines and fibroblastic cell lines were found to grow well on collagen type I-fibronectin, collagen type I, and fibronectin coated glass surface to compare with other materials employed in this study. Collagen-fibronectin and collagen-coated alumina ceramic tubes were embedded in the mandibular bones of rabbits, and the aspect was observed with the passage of time. These treatments promoted the osseous area in the tubes. These findings strongly indicated that surface-coated biomaterial by collagen and collagen-fibronectin enhanced cellular adhesion, and promoted the growth of fibroblast and osteoblast in vitro and in vivo.

Animals↗
PubMed1990

[Scoring system for screening of patients with endometriosis in infertile clinic].

An outpatient department screening method for endometriosis was prepared by designing a scoring system in a retrospective study. We compared the incidence of 21 characteristic symptoms and the finding of endometriosis in 39 infertile women with endometriosis diagnosed by laparoscopy or laparotomy and 21 women without it. A table for diagnostic scoring composed of 11 items was accomplished. The feasibility of this diagnostic scoring system was then examined in a prospective study of 72 women with endometriosis and 47 without it. The results were as follows. 1. In the retrospective study, all the women with 10 or more points had had endometriosis. Fifteen out of 20 women with -20 points or less had been without endometriosis and the remaining 5 with endometriosis (4 minimal and 1 mild stage according to AFS classification). 2. In the prospective study, a 94.5% (52/55) incidence of endometriosis was found in women with 10 or more points and 66.7% (10/15) of women with -20 points or less were without endometriosis. 3. This diagnostic scoring system, in which women with endometriosis score 10 or more points and women without it -20 points or less, has 91.2% sensitivity, 76.9% specificity, 94.5% positive predictive value and 66.7% of the negative predictive value. It is concluded that this scoring system has clinical diagnostic value in screening for endometriosis in infertile women at the outpatient department.

Adult↗
PubMed1990

[Correlation of CT and pathologic findings of pulmonary lymphangioleiomyomatosis].

The X-ray CT findings of two cases of pulmonary lymphangioleiomyomatosis were reported. The correlation between high-resolution CT findings and inflated biopsy specimens was studied. The X-ray CT findings are 1) multiple low attenuation areas, 2) diffuse areas of slightly increased density and 3) irregular enlargement of pulmonary vascular images. Each low attenuation are turned out to correspond to emphysematous lesions. Slightly increased densities on CT images seemed to be caused by a summation of many small nodules of a proliferation of smooth muscle cells located in the wall of respiratory bronchioles and alveolar ducts, with or without intraalveolar hemosiderosis. Some nodular lesions in bronchiolar walls were so close to neighboring vessels that they could not be separated from vascular images on CT, so peripheral vascular images were irregularly thickened. X-ray CT reflected more actual pathological findings than routine chest radiographs. As low attenuation areas on CT images have been reported to be representative of pulmonary emphysema, it is thought that the above CT findings must be differentiated from those of pulmonary emphysema. While pulmonary vascular images were thin and stretched on the CT in patients with emphysema, they were irregularly thickened on the CT of patients with LAM. Furthermore, while CT of emphysema often revealed overinflation or decreased density, diffuse areas of slightly increased density were never found in emphysema.

Adult↗
PubMed1990

[Clinical statistics of the bladder tumor--clinical and pathological aspects of 325 cases].

We analyzed 325 primary bladder tumor patients who were treated in our hospital during the past 15 years. There were 242 males and 83 females who were between 20 and 84 years old with an average age of 63 years old. The most frequent complaint was macroscopic hematuria in 76.9% of the patients (250/325). Cystoscopically, a single tumor was seen in 71%, the tumor was medium sized (1 to 3 cm) in 32%, and 56% were papillary tumors with a stalk. Histologically, 300 (92.3%) cases were transitional cell carcinoma. There were 42, 206 and 52 patients with grades 1, 2 and 3 transitional cell carcinoma and stage Tis, Ta, T1, T2, T3a, T3b, T2-4M1 and Tx were 2,46, 151, 30, 15, 16, 37 and 3 cases each. Transurethral resection was performed in 231 (71.1%), partial cystectomy in 6 (1.8%) and total cystectomy in 44 (12.5%) cases each.

Adenocarcinoma↗
PubMed1990

[Clinical statistics of the bladder tumor--survival rates of 325 cases].

To study the relationship between clinical features and prognosis of the bladder tumor, 325 patients who were treated in our hospital were analyzed. The overall 5-year and 10-year actual survival rates were 65% and 51%. There was no statistical significance in the actual survival rate between the single and multiple tumor group. Although, there was a significant difference in tumor size, tumor growing type and the mode of treatment. The ten-year survival rates for grades 1, 2 and 3 cases were 76%, 57% and 18%, respectively. There was a significant difference between grades 1 and 3 (p less than 0.001) and grades 2 and 3 (p less than 0.001). The ten-year survival rates for stages Ta, T1, T2, T3a, T3b and T2-4 M1 were 79%, 68%, 43%, 0%, 19% and 5%, respectively. There was a significant difference between the Ta, 1 and T2-4 group (p less than 0.001).

Adult↗
PubMed1990

[Changes in the immune response in postoperative patients while running the ventricular assist device (VAD)].

We examined the immunological parameters in two patients who were assisted with LVAD in postoperative LOS. Both patients demonstrated transient consumption of complements immediately after running the LVAD. The serum concentrations of immunoglobulins (IgG, IgM, IgA) showed no significant decrease. Both patients had significant postoperative lymphopenia, but recovered in a week. A decrease in the number of T cells was observed in both patients. The OKT4/OKT8 ratio initially increased in one patient, but gradually declined postoperatively. In the other patient, the OKT4/OKT8 ratio decreased. The lymphocyte response to mitogen (PHA) remained significantly depressed when the number of lymphocytes returned to the normal level, suggesting that the lymphocytes were functionally impaired. Such findings are generally observed after cardiac surgery. Thus, LVAD does not affect the immune response.

Adolescent↗
PubMed1990

Effect of chitin heparinoids on the activation of peritoneal macrophages and on the production of monokines in mice.

Sulfonated derivatives of chitin which showed anticoagulant activity (chitin heparinoids) were studied with regard to the activation of mouse peritoneal macrophages and the production of monokines. In comparison with 70% deacetylated chitin (DAC-70), which was the most adjuvant-active derivative of chitin, all chitin heparinoids were less effective for the augmentation of cytolytic activity of peritoneal macrophages. The number of macrophages was hardly increased or decreased by intraperitoneal injection of chitin heparinoids, and the activity of circulating colony-stimulating factor was not changed by their treatment. Only N-sulfonated DAC-70 stimulated the production of interleukin-1 by thioglycolate-induced peritoneal macrophages in vitro. However, its effect was weaker than that of DAC-70. Chitin heparinoids showed no or weak mitogenic activity on normal mouse spleen cells.

Adjuvants, Immunologic↗
PubMed1990

[Clinical experience with ceftibuten in complicated urinary tract infections].

Twenty-four patients with complicated urinary tract infections (UTI) were received ceftibuten (CETB) 400 mg b.i.d. orally for 5 days, and the following results were obtained. One of the recipients was excluded for negative bacteriuria before treatment. According to the criteria for evaluation of clinical efficacy of antimicrobial agents on UTI, 23 patients were assessed: 9 of them were judged to show excellent responses, 6 to have moderate responses and 8 poor responses. The overall efficacy was 65%. Four patients with indwelling catheter failed to show positive clinical response, but 15 out of 19 patients without indwelling catheter responded positively. As to bacteriological responses, organisms included Gram-positive cocci were eradicated in 28, replaced in 9 and persisted in 6 strains. Of 24 Gram-negative rods except 1 strain of Pseudomonas sp., 23 (96%) were eradicated. Safety evaluation was made in all 24 patients received CETB. No adverse reactions were observed in any cases. As to laboratory test results, slight, transient elevations of GOT, GPT and Al-P of serum were noted in 1 patient. Their values, however, returned to normal levels in 4 weeks.

Adult↗
PubMed1989

Assessment of transesophageal Doppler echography in dissecting aortic aneurysm.

To assess the clinical value of transesophageal Doppler echography in the diagnosis of dissecting aortic aneurysm, both transesophageal and conventional echograms were performed in 22 cases of dissecting aortic aneurysm. Of the 22 patients, 17 underwent angiography; 8, X-ray computed tomography; 4, both; and 12, surgery. The performance of each method was assessed in the following four segments: A, ascending aorta; B, aortic arch; C, thoracic descending aorta; and D, upper abdominal aorta. The results by angiography were presumed to be correct. In the group of 17 patients who underwent angiography, the rate of correct detection of an intimal flap using the transesophageal approach was 100% in all four segments, significantly better than detection by the conventional approach (segment A, 65%; segment B, 47%; segment C, 35%; segment D, 53%) (p less than 0.01), and the rate of correct detection of the entry sites using the transesophageal approach was 100%, significantly better than that by conventional approach (42%) (p less than 0.05). X-ray computed tomography was not capable of detecting the site of entry in all cases. The presence of thrombus, aortic regurgitation and pericardial hemorrhage were all revealed clearly by the transesophageal approach, and the results were partly proved by other methods. In conclusion, transesophageal Doppler echography provides a rapid and accurate method of diagnosing and evaluating dissecting aortic aneurysm and permits prompt initiation of appropriate treatment.

Adult↗
PubMed1989

Effects of D-serine on bacterial D-amino acid transaminase: accumulation of an intermediate and inactivation of the enzyme.

Incubation of pure bacterial D-amino acid transaminase with D-serine or erythro-beta-hydroxy-DL-aspartic acid, which are relatively poor substrates, leads to generation of a new absorbance band at 493 nm that is probably the quinonoid intermediate. The 420-nm absorbance band (due to the pyridoxal phosphate coenzyme) decreases, and the 338-nm absorbance band (due to the pyridoxamine phosphate or some other form of the coenzyme) increases. A negative Cotton effect at 493 nm in the circular dichroism spectra is also generated. Closely related D amino acids do not lead to generation of this new absorption band, which has a half-life of the order of several hours. Treatment of the enzyme with the good substrate D-alanine leads to a small but detectable amount of the same absorbance band. D-Serine but not erythro-beta-hydroxyaspartate leads to inactivation of D-amino acid transaminase, and D-alanine affords partial protection. The results indicate that D-serine is a unique type of inhibitor in which the initial steps of the half-reaction of transamination are so slow that a quinonoid intermediate with a 493-nm absorption band accumulates. A derivative formed from this intermediate inactivates the enzyme.

Circular Dichroism↗
PubMed1989

Stereospecificity of reactions catalyzed by bacterial D-amino acid transaminase.

The spectral shift from 420 to 338 nm when pure bacterial D-amino acid transaminase binds D-amino acid substrates is also exhibited in part by high concentrations of L-amino acids (L-alanine and L-glutamate) but not by simple dicarboxylic acids or monoamines. Slow processing of L-alanine to D-alanine was observed both by coupled enzymatic assays using D-amino acid oxidase and by high pressure liquid chromatography analysis employing an optically active chromophore (Marfey's reagent). When the acceptor for L-alanine was alpha-ketoglutarate, D-glutamate was also formed. This minor activity of the transaminase involved both homologous (L-alanine and D-alanine) and heterologous (L-alanine and D-glutamate) substrate pairs and was a function of the nature of the keto acid acceptor. In the presence of alpha-ketoisovalerate, DL-alanine was almost completely processed to D-valine; within the limits of the assay no L-valine was detected. With alpha-ketoisocaproate, 90% of the DL-alanine was converted to D-leucine. In the mechanism of this transaminase reaction, there may be more stereoselective constraints for the protonation of the quinonoid intermediate during the second half-reaction of the transamination reaction, i.e. the donation of the amino group from the pyridoxamine 5'-phosphate coenzyme to a second keto acid acceptor, than during removal of the alpha proton in the initial steps of the reaction pathway. Thus, with this D-amino acid transaminase, the discrete steps of transamination ensure fidelity of the stereospecificity of reaction pathway.

Bacillus↗