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Biomedical subjects

K Nagata

Publications and source records attributed to K Nagata.

At least 1,063 records · Page 59Linked to original sources
PubMed1981

Monocyte function in idiopathic nephrotic syndrome in childhood.

The monocyte function in 2-15 year old patients with idiopathic nephrotic syndrome (INS) in childhood was studied in reference to the phagocytosis of sensitized red blood cells and NBT reduction. The monocyte function was found to be increased in fresh cases of INS. In the case of refractory INS not responding to steroid treatment, the monocyte function was normal or tended to be low.

Adolescent↗
PubMed1980

Transesophageal cross-sectional echocardiography.

A transesophageal cardiac imaging system is described. This system employs hand-held mechanical sector and linear scanners each having a flexible tube and a small ultrasonic transducer contained within a small oil bag easily swallowed by adults. In the sector scanner, a small transducter in the esophagus rotates alternately and horizontal heart images are displayed. In the linear scanner, a small transducer in the esophagus moves up and down and vertical heart images are displayed. The system was evaluated in 31 adult subjects. In all subjects, stable high quality heart images were observed continuously from base to apex as the transducer was being withdrawn or entire heart images were observed at the level of the atrioventricular valves. In vertical scans, the bifurcation of the pulmonary artery could be observed clearly. There was little difference in the image quality among subjects.

Adult↗
PubMed1980

Clinical analysis on steroids. XII. Occurrence of D-homoannulation during the hot acid hydrolysis of 5 beta-pregnane-3 alpha, 20 alpha-diol disulfate.

Two D-homosteroids were isolated from the hydrolyzate of 5 beta-pregnane -3 alpha, 20 alpha-diol disulfate (II) when it was refluxed in 3N hydrochloric acid. The structures of these steroids have been elucidated as 17 alpha-methyl-D-homo-5 beta-androstane-3 alpha, 17a beta-diol (VI) and 17 alpha-methyl-17a beta-chloro-D-homo-5 beta-androstan-3 alpha-ol (VIII) by instrumental analyses. The former was identical with a synthetic specimen derived from 5 beta-pregnane-3 alpha, 20 beta-diol disulfate (IV) by uranediol rearrangement. The main hydrolyzates obtained were 17 alpha-ethyl-17 beta-methyl-18-nor-5 beta-androst-13-en-3 alpha-ol (V) and 5 beta-pregnane-3 alpha, 20 alpha-diol (III).

Androstane-3,17-diol↗
PubMed1980

Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin.

Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.

Actins↗
PubMed1980

Interaction of parotid saliva basic glycoprotein with Streptococcus sanguis ATCC 10557.

It is postulated that an initial step in dental plaque formation is the adherence of oral bacteria to the salivary pellicle. Recently, we have found that a proline-rich and basic glycoprotein (MGP) from human parotid saliva, which is successfully purified by Concanavalin A-Sepharose affinity chromatography, binds to some oral streptococci such as S. mitis and S. sanguis. This paper deals with some inhibitors which affect the binding of the MGP to S. sanguis ATCC 10557. The assay for the binding ability of the radioactive MGP to the bacterial cells was performed by incubation of the reaction mixture containing 10 microgram of [3H]MGP (6000 dpm) and about 4.5 mg of bacterial cells (dry weight) in 0.5 ml of 0.05 M Tris-HCl buffer containing 0.05 M NaCl, pH 8.0 with a final volume of 0.51 ml. After 1 hour standing at 4 degrees C, the cells were washed five times with the same buffer. The resulting sediment was solubilized in 0.5 ml of NCS tissue solubilizer and the radioactivity was measured. The binding of the radioactive MGP to bacterial cells was specifically inhibited by galactose, lactose and N-acetyllactosamine. Applications of heat and trypsin on the cell surface, strikingly reduced the binding ability. These findings strongly suggested that a lectin-like substance may be present on the bacterial cell surface.

Adult↗
PubMed1980

[Preparation of hard crown and bridge resin without PMMA powder (III) (author's transl)].

We have been studying hard crown and bridge resins. Mixtures of several polyfunctional monomers and a composite filler finely pulverized, prepared by polymerization of trimethylolpropane trimethacrylate in the presence of hydrophobic colloidal silica, were polymerized and their mechanical properties were studied. They were excellent and a sample from 2.6 E.UDMA comonomer was the best among them. The new filler possessed better affinity with matrices than composite fillers reported previously, presumably due to the reactivity with matrices. Introduction of this new filler into the hard crown and bridge resin preparation must improved the resins remarkably.

Crowns↗
PubMed1980

[Clinical studies of cefoperazone in pediatric field (author's transl)].

Clinical studies of cefoperazone (CPZ) were performed and the following results were obtained. 1) CPZ was intravenously administered at the daily dose of 35 approximately 107 mg/kg to 10 children with infection: urinary tract infection (6 cases), respiratory infection (3 cases), and acute enterocolitis (1 case). The overall efficacy rate was 80%, and in urinary tract infection including one case caused by Pseudomonas aeruginosa, efficacy rate was 100%. 2) The bloody stools were noted in one case, 2 days after the administration of CPZ, but improved gradually. No abnormal laboratory data were found.

Adolescent↗
PubMed1980

[Heat treatment of gold alloy to get adhesion with resin (author's transl)].

Combination of gold alloy and resin is common to prepare prostheses and restorations in dentistry. However, durable adhesion between gold alloy and resin has not been achieved, and mechanical retention which possesses many disadvantages is indispensable to fix them with each other. In our previous report, we had prepared a 4-META adhesive opaque resin and made sure of its excellent adhesiveness to dental non-precious alloy. Here, we developed a new method to change gold alloy surface adhesive and studied the adhesion between 4-META opaque resin and the modified alloy surface. The treatment is consisted of heat treatment and acid pickling. 4-META opaque resin could adhere strongly to treated gold alloys and the adhesive bond strength was 250 Kg/cm2. Moreover, durability of the adhesion is excellent and the value did not decreased even after subjected to 2000 cycle of thermal cycling (4 degrees C and 60 degrees C) or long immersion in water at 37 degrees C.

Adhesiveness↗
PubMed1980

[Fundamental and clinical studies of sustained release cephalexin (L-Keflex) in the field of oral surgery (author's transl)].

L-Keflex is a newly manufactured cephalexin product in order to maintain effective blood level of the drug for a long period of time. The results of the fundamental and clinical studies are as follows: 1. Mean blood levels of the drug after its single oral dose of 1 g in fasting in 22 cases before operations were: 7.19 mcg/ml at 2 hours (peak), 4.35 mcg/ml at 4 hours, 4.21 mcg/ml at 6 hours, 2.47 mcg/ml at 8 hours and 1.81 mcg/ml at 12 hours, respectively. Existence of the drug in blood was observed for a long period of time. 2. The distribution into the tissues of L-Keflex was generally good. The tissue levels in 19 of 22 samples ranged from 1.30 to 18.0 mcg/g, but 3 samples did not detect the drug in the tissues. Tissue level/blood level ratios were 0.19 approximately 2.67. 3. Half of 30 cases with mild dental infections was treated with a daily dose of 1 g with clinical response of 60.0%, and the other half with 2 g was 78.6% in clinical response. The overall efficacy rate was 69.0%. 4. As a side effect of the drug, only one of 52 cases (fundamental 22 cases, clinical 30 cases) complained of edema on both eyelids. No. abnormality was observed in blood finding, hepatic and/or renal function. From the above results, it is considered that L-Keflex is an effective antibiotic product in infections in the field of oral surgery. Also, L-Keflex has an advantage in that its administration frequence (b.i.d) is less than that of regular cephalexin (q.i.d.).

Adolescent↗
PubMed1980

Effects of niludipine on regional myocardial blood flow and regional myocardial function in the dog with partial occlusion of the coronary artery.

The effects of bis(2-propoxyethyl)-1,4-dihydro-2,6-dimethyl-4-*3-nitrophenyl)-3,5-pyridine dicarboxylate (niludipine), a newly-synthesized calcium antagonist related in chestical structure to nifedipine, on the ischemic myocardium were studied in eight anesthetized, open-chest dogs, mainly by the simultaneous measurement of regional myocardial blood flow (MBF) and regional myocardial function. The ischemic myocardium was prepared by partial occlusion of the left circumflex coronary artery, and niludipine was given i.v. at a dose of 5 micrograms/kg. MBF was measured by the radioactive microsphere method and regional myocardial function was assessed by measurement of myocardial segment length. Measurements were also taken of aortic pressure, left ventricular pressure, ascending aortic blood flow, and left circumflex coronary blood flow. Niludipine decreased left ventricular contractility, dilated resistance vessels and reduced heart rate. MBF, measured 5 min after the drug was administered, increased in both the ischemic and the non-ischemic myocardium. Improvement in regional myocardial function was observed in the ischemic myocardium 5 min later, when aortic pressure had returned to nearly the pre-administration level. Thus, the results of the study suggest that niludipine improves the balance between myocardial energy supply and demand in the ischemic myocardium when there is little change in aortic pressure, and we concluded that niludipine exerts a beneficial effect on the ischemic myocardium

Animals↗