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Biomedical subjects

K Nabeshima

Publications and source records attributed to K Nabeshima.

At least 109 records · Page 6Linked to original sources
PubMed1986

Glycosaminoglycan in malignant pleural mesothelioma.

The quantitative analysis on glycosaminoglycan (GAG) in the tumor tissues of five patients with malignant pleural mesothelioma and in the pleural fluid of two patients was performed with the use of biochemical methods. In the tumor tissues, it was found that the average of the total amount of GAG was more than 7.9 times as high as that in adenocarcinoma of the lung, and that hyaluronic acid and chondroitin sulfate were main constituents of mesothelioma GAG. However, there was no significant difference in the content of dermatan sulfate and heparan sulfate between this neoplasm and adenocarcinoma. In the pleural fluid, the amount of hyaluronic acid was about 40 to 230 times higher than that in adenocarcinoma of the lung with the increment of chondroitin sulfate (11-87 times). These findings suggest that a marked increase in the total amount of GAG and the elevation of either the hyaluronic acid or the chondroitin sulfate level, or both, are characteristic abnormalities in malignant pleural mesothelioma.

Adult↗
PubMed1986

Partial purification and characterization of serum protease from tumor-bearing rats which cleaves type IV collagen.

Activity of neutral protease was increased in sera of rats bearing ascites hepatoma AH109A compared to those of normal rats. The protease was isolated from serum protein and partially purified approximately 1,150 times in specific activity after sequential column chromatography of hemoglobin affinity, lysine-Sepharose, Ultrogel AcA34 and TSK-gel G2000SW in that order. The protease fraction still seemed to contain at least two kinds of proteases, serine and cysteine protease. It had a molecular weight of 18-21 kilodaltons with broad optimal pH range of 7.0-9.0, maximum at 8.0. Intradermal injection of the crude preparation of the neutral protease fraction induced extravascular emigration of circulating tumor cells in vivo. Moreover, partially purified protease degraded pepsin-treated chains of bovine glomerular type IV collagen in vitro, but such an in vitro action of the protease was inhibited by an addition of soybean trypsin inhibitor or mercuric chloride. It failed to cleave salt-extracted rat skin type I collagen under the same digestive conditions for bovine type IV collagen. The serum neutral proteases of tumor-bearing host may play some cooperative roles during extravascular emigration of tumor cells by destruction of vascular basement membrane.

Animals↗
PubMed1986

Enhanced migration of tumor cells in response to collagen degradation products and tumor cell collagenolytic activity.

The migration of ascites and cultured hepatoma cells, AH109A of rat (Donryu) origin and MH134 of mouse (C3H), was enhanced by collagen degradation products (CDP) in vitro using the modified Boyden chamber, but not by collagen. Both tumor cells demonstrated somewhat increased motility in the presence of CDP regardless of whether or not there was a gradient present, but the maximum response was seen in the presence of a gradient. MH134 cells responded more effectively to CDP than AH109A cells and showed similar migratory responses to type I and IV collagen degradation products (CDP-I and -IV). Synthetic di- or tripeptides containing hydroxyproline were less chemotactic for MH134 cells than CDP-I and -IV. Both proteases (collagenase and trypsin) and MH134 cells could degrade a collagen substrate and generate CDP. These findings suggest that CDP released during the process of invasion may play a role in the migration of tumor cells and consequent formation of metastases.

Animals↗
PubMed1985

[Effect of adriamycin, mitomycin-C, and tegafur (AMF) chemotherapy on advanced lung cancer].

Seven patients with advanced inoperable carcinoma of the lung were treated with combination chemotherapy consisting of adriamycin, mitomycin-C, and tegafur (AMF therapy). There was, at least, no progress of the carcinoma during treatment and objective response was obtained in 28.6% of patients. With regard to side effects, leukopenia and gastrointestinal symptoms were found in each of 3 patients.

Adenocarcinoma↗
PubMed1985

[The effect of lithium carbonate against leukopenia during systemic chemotherapy in patients with small cell carcinoma of the lung].

To investigate whether lithium carbonate ameliorates the leukopenia and infectious complication that accompany systemic chemotherapy, we studied 19 patients with small cell carcinoma of the lung receiving combination chemotherapy. Eight patients received systemic chemotherapy and lithium carbonate and 11 patients received systemic chemotherapy alone. The mean leukocyte count nadir during chemotherapy was significantly higher in the patients of the lithium group than in the patients of the control group (p less than 0.05). Percentage of infectious complication related to leukopenia was lower in the lithium group than in the control group, although there was no significant difference between these two groups. There was almost no significant side effect except for liver dysfunction in one patient. We therefore believe that lithium carbonate is an effective and safe drug against leukopenia during cytotoxic chemotherapy.

Aged↗
PubMed1985

Hypercholinesterasemia with isoenzymic alteration in a family.

A family with hypercholinesterasemia with isoenzymic alteration is reported. The propositus, a 55-year-old woman, was admitted to our hospital because of diabetes mellitus. Because her cholinesterase activity (delta pH 3.2) was supranormal, with no other abnormal liver-function test result throughout the hospitalization period, and was independent of her disease state, we investigated whether this condition might be familial. We studied six of her 17 family members in three generations. All six had above-normal serum cholinesterase activity. Gradient gel electrophoresis on polyacrylamide showed that the normal control individuals had seven isoenzymes, but all the family members with hypercholinesterasemia had two additional isoenzymes. The enzymic properties of the affected members were similar to those of the normal individuals. Hypercholinesterasemia in this family seems to be the result of an increased number of enzyme molecules, but how this isoenzymic alteration emerged remains obscure.

Alcohols↗
PubMed1984

[Undifferentiated adenocarcinoma of the lung associated with sarcoidosis--differential diagnosis].

A 51-year-old male with undifferentiated adenocarcinoma of the lung associated with sarcoidosis is reported. Scalenus node biopsy and open thoracotomy revealed sarcoid granuloma. High levels of serum angiotensin-converting enzyme and ocular findings also suggested sarcoidosis. No malignant cells were recognized by repeat lymph node biopsy. However, post-mortem examination showed bronchial carcinoma; no sarcoid granuloma was found. The diagnostically difficult points of our case are discussed.

Adenocarcinoma↗