Search PubMed⌕ Search

Biomedical subjects

K Murota

Publications and source records attributed to K Murota.

At least 37 records · Page 2Linked to original sources
PubMed1989

Effects of NSAID on collagen and proteoglycan synthesis of cultured chondrocytes.

Three major NSAID (tiaprofenic acid, indomethacin and acetylsalicylic acid) were studied with regard to their effects on the biosynthesis and gene expression of collagen and proteoglycan of chondrocytes. We found that the biosynthesis of type 11 collagen was suppressed in 60-70% of the controls by either indomethacin or acetylsalicylic acid; tiaprofenic acid did not suppress collagen synthesis. Although the biosynthesis of proteoglycan was suppressed to 80-90% of the control by indomethacin or acetylsalicylic acid, tiaprofenic acid did not suppress proteoglycan synthesis. In order to investigate the gene expression of type 11 collagen and proteoglycan, dot blot hybridization was performed using type 11 collagen and proteoglycan cDNA. The effects of NSAID on the amount of type 11 collagen and proteoglycan mRNA were consistent with those on collagen and proteoglycan biosynthesis. Thus, we concluded that tiaprofenic acid has less effect on cultured chondrocytes.

Animals↗
PubMed1988

Effect of temperature on antibacterial activity of lidocaine to Staphylococcus aureus and Pseudomonas aeruginosa.

The effect of temperature on the antibacterial activity of lidocaine to Staphylococcus aureus and Pseudomonas aeruginosa was investigated in vitro. At 10 C at which S. aureus organisms do not grow and might be metabolically inactive, the antibacterial activity of lidocaine to S. aureus was not observed in a concentration of 1%, which was quite antibacterial to S. aureus at 37 C. On the other hand, at 40 C a conspicuously increased antibacterial activity to S. aureus of lidocaine was observed in a concentration of 0.25% which was not antibacterial to S. aureus organisms at 37 C. Similar results were obtained when P. aeruginosa organisms were examined in place of S. aureus, although P. aeruginosa was found to be less susceptible to lidocaine than S. aureus. The clinical significance of the thermal effect on the antibacterial activity of lidocaine was discussed in brief.

Drug Resistance, Microbial↗
PubMed1986

Isolation of peripheral nerve collagen.

A new method for the isolation of high yields of collagen from human peripheral nerve is described. A major technique adopted in the present work is sedimentation of the tissue homogenates in a sucrose density gradient. The defatted fibrous material isolated after the removal of myelin was shown to be a relatively pure collagenous substance by amino acid analysis, indicating that the removal of noncollagenous proteins, especially glycoproteins, from collagen fibrils was effectively achieved by this method. The yield of collagen at this step was more than 90% of the total collagen in peripheral nerve. Subsequent extractions with solutions of neutral saline and sodium citrate were found to give further purification of the collagenous protein. The collagens from embryonic peripheral nerves were composed of Type I and III collagens, while Type III collagen was found to be less abundant in adult peripheral nerves.

Adult↗
PubMed1983

Abnormal collagen synthesis in skeletal muscle of dystrophic chicken.

Specific molecular properties of skeletal muscle collagens from normal and dystrophic chickens have been compared. When dystrophy develops in skeletal muscle tissue there was an increase in the amount of total collagen and an increased proportion of Type III collagen in the tissue. The results from the cross-link study as well as the analysis of the solubility of collagen showed that skeletal muscle of dystrophic chicken produces more immature collagen fibers compared to normal chicken. These findings strongly indicate an important role of collagen in the pathogenesis of the extensive connective tissue prolipheration characteristic of muscular dystrophies.

Animals↗