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Biomedical subjects

K Murakami

Publications and source records attributed to K Murakami.

At least 1,351 records · Page 75Linked to original sources
PubMed1975

Crystallization and some properties of purine nucleoside phosphorylase from chicken liver.

Purine nucleoside phosphorylase (purine nucleoside:orthophosphate ribosyltransferase, EC 2.4.2.1) from chicken liver has been purified about 650 fold and crystallized. The crystalline enzyme was cube shaped and showed a specific activity of 46 units per mg of protein. The homogeneity of the crystalline enzyme was shown by polyacrylamide gel-disc electrophoresis. The sedimentation coefficient (s-degrees 2o,w) was 5.4 S. The crystalline enzyme was activated by the substrate inosine. The Hill coefficient was estimated to be 0.76, suggesting negative cooperativity with regard to the substrate inosine. The results of the kinetic analysis are consistent with the mechanism being a "rapid equilibrium random Bi-Bi reaction". The apparent equilibrium constant for phosphorolysis was 0.048.

Animals↗
PubMed1975

Further studies on the existence of a sensitizing factor to pressor agents in hypertension.

Further studies are reported on the existence of a sensitizing factor in plasma of hypertensive subjects, which increases the vascular sensitivity to pressor agents when injected iv into nephrectomized rats. Plasma samples from normotensive subjects, patients with malignant hypertension, normotensive dogs, and dogs with experimental renovascular hypertension were fractionated on Bio-Gel P-10 columns after cold acetone precipitation, and on DEAE-cellulose columns eluted with sodium chloride and pH gradients. The effect of the various fractions on the vascular sensitivity to angiotensin was tested utilizing nephrectomized rats. The sensitizing activity was found only in fractions obtained from plasma of hypertensive patients and dogs and it was concentrated primarily in three fractions. Th results suggest that the sensitizing factor is negatively charged at neutral pH and it could be a polypeptide or a small protein.

Angiotensin II↗
PubMed1975

Study of an intravenous fat tolerance test with Intralipid. II. The relation between K2 and PHLA with special reference to clinical data in human subjects.

The relation between K2 and PHLA was studied in human subjects with special reference to clinical data determined by routine laboratory and physical examinations. The results obtained by Multiple Regression Analysis indicated that those factors which may contribute to K2 variation were fasting triglyceride level and age. There was an inverse partial correlation between K2 and fasting triglyceride level and between K2 and age. The first and second principal components calculated by Principal Component Analysis indicated that K2 is closely related to obesity and hyperlipidemia, especially hypertriglyceridemia, while PHLA related to albumin. These two components also suggested that K2 fibes different clinical information from that obtained by PHLA measurement. There was no partial correlation between K2 and PHLA. The various lipoprotein paper electrophoretic patterns, type IIa, type IIb, type IV and normal patterns, were clearly characterized by such factors as K2, plasma triglyceride and degree of obesity which has high coefficients in the first principal component.

Adult↗
PubMed1975

A variant of hexokinase isoenzymes detected in ascites tumor cells.

In the hexokinase isoenzyme pattern on the cellulose acetate membrane electropherogram of the supernatant fraction of Ehrlich ascites tumor cells, a band was detected moving slower than of common Type I isoenzyme. This band was assumed to be due to the presence of a variant of the hexokinase isoenzymes in the cells, since such a band was not detected in the normal tissues, and a change in the intensity of the band in response to a sulfhydryl agent, etc., differed from that of the common Type I and II isoenzymes. The band was also found in AH-7974 and MH-134 ascites tumor cells, in spite of the difference in the species of the tumor-bearing animals and originating tissue.

Animals↗