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K Moriguchi

Publications and source records attributed to K Moriguchi.

At least 19 recordsLinked to original sources
PubMed1992

Increase of methionine aminopeptidase activity in hyperplastic Leydig cells of rat cryptorchid testis: a histochemical study.

Histochemical study on the changes of the aminopeptidase activities in rat testes after surgically-induced cryptorchidism was conducted comparing them with the histochemical changes in regenerated hepatic cells of the partially hepatectomized rat liver. Methionine-aminopeptidase in Leydig cells gradually increased after cryptorchid was induced, whereas the enzyme activity in regenerated hepatic cells decreased. These histochemical observations were coincident with the data obtained by enzyme assay. The present study has indicated that in the rat cryptorchid testis the increase of methionine-aminopeptidase activity was caused by hyperplastic Leydig cells.

Aminopeptidases↗
PubMed1991

Human neutrophils produce free radicals from the cell-zymosan interface during phagocytosis and from the whole plasma membrane when stimulated with calcium ionophore A23187.

The production of free radicals, superoxide anions (O2-), and hydrogen peroxide (H2O2) was histochemically investigated in human neutrophils that were stimulated by either phagocytosis or the calcium ionophore A23187. To demonstrate O2-, peripheral neutrophils from healthy donors were incubated at 37 degrees C in a medium containing nitroblue tetrazolium and glucose in the presence of either opsonized zymosan A and/or A23187. To demonstrate H2O2, neutrophils pretreated with a stimulant for 10 min were washed and incubated in a cerium medium containing CeCl3 and glucose in a Tris-maleate buffer. In cells engaged in phagocytosis, diformazan (for O2-) and cerium perhydroxide deposits (for H2O2) were restricted to the neutrophil-particle interface and on the inner surface of phagosomes. The remaining free surface of the plasma membrane was devoid of reaction products. In the case of neutrophils stimulated with A23187, the production of O2- and H2O2 was visualized over the whole surface of the plasma membrane. These histochemical reactions were inhibited by p-benzoquinone, superoxide dismutase, ferricytochrome c or catalase, and p-diazobenzenesulfonate (a membrane-impermeable protein denaturant). The results showed that human neutrophils produce free radicals exocellularly and that the site of production varies with different stimuli.

Azo Compounds↗
PubMed1991

Proventricular glands in fowl.

Some researchers have already described the fowl proventriculus. However, we believed there was a need for detailed carbohydrate histochemical investigations on the same glands. Moreover, some researchers had erred about the lamina muscularis mucosae. The results of these investigations are as follows. 1. The proventricular glands consist of both superficial and profound gastric glands. 2. The superficial glands are distributed in the lamina propria mucosae while the profound glands exist in the tela submucosa. 3. The superficial glands are simple, branched tubular glands. The columnar glandular cells are arranged in a simple layer and react strongly to PAS, AB (pH 2.5 and 0.5). These appear to be dark purple when they are stained with PAS-AB (pH 2.5). Some other methods have also been tried. 4. Judging from the data 3), the superficial gastric glands contain neutral, weak and strong acids, sulfuric and acid mucopolysaccharides, sialomucin, and II and III neutral mucus type. 5. Glandular cells in the body and basal portions of the superficial gastric glands contain a large number of fine pepsinogen granules. 6. Judging from the data of 3)-5), we believe that the superficial gastric glands are undifferentiated gastric glands and that they are same kinds of glands that are found in mammals. 7. A large number of profound gastric glands fill the tela submucosa. They are compound tubular glands, and are composed of many glandular alveoli. Their columnar glandular cells are arranged in a simple layer. 8. These glandular cells react moderately to PAS, negatively to AB (pH 2.5 and 0.5) and PAS-AB (pH 2.5). Moreover, we observed some other reactions.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

Pepsinogen granules in the esophageal epithelium of the rock snake.

We demonstrated a large number of pepsinogen granules in the esophageal pseudostratified ciliated epithelium of the rock snake. 1. The snake esophagus is covered with a pseudostratified ciliated epithelium. 2. This epithelium develop mostly in the upper portion of the esophagus. 3. Long supranuclear portions of the same cells are strongly PAS-positive and contain pepsinogen granules. Those cells possess cilia. 4. The strongly PAS-positive cells and pepsinogen granules decrease or are lost in the middle and lower portions of the esophagus. 5. Glands are distributed in the lamina propria mucosae of the esophagus of the Japanese lizard and gecko. Those in humans and the bird are compound tubular glands and those of the Japanese lizard and gecko are bottle-shaped. The pepsinogen granules of these glands are secreted into the excretory ducts and then discharged into the esophageal lumen. 6. However, the same granules of the snake are contained in the supranuclear portion of the epithelium and are secreted directly into the esophageal lumen. 7. The mode of pepsinogen granule secretion of the esophagus is most simple in the snake.

Animals↗
PubMed1991

Glands distributed in the lamina propria mucosae of the esophagus in the gecko and Japanese lizard.

In an earlier study, we found compound tubular glands distributed in the lamina propria mucosae of human and fowl esophagus. Subsequently, we discovered bottle-shaped glands in the Japanese lizard and gecko esophagus in the same lamina as that of the human and fowl. Moreover those glands produced equivalent pepsinogen granules. We provide below, a detailed description on the results. 1. Bottle-shaped glands were distributed in the lamina propria mucosae of the Japanese lizard and gecko esophagus. 2. A large number of those glands were distributed in the lower region of the esophagus, but did not exist in the upper and middle regions of the esophagus. 3. The esophageal mucous membrane of the gecko and Japanese lizard were covered with a simple columnar ciliated epithelium, and the same epithelium reacted strongly to PAS and AB (pH 2.5), moderately to AB (pH 0.5) or negatively. 4. PAS-AB (pH 2.5) stain presented a dark blue color or a deep red color or a deep red and dark blue mixed color in one section. 5. The above-mentioned glands contained pepsinogen granules. 6. Those glands do not possess parietal cells.

Animals↗
PubMed1990

Do the cardiac glands exist? 5. The dog.

The authors performed histological and histochemical investigations on the so-called cardiac glands and gastric glands of the dog, and compared the data on these glands. The conclusions were as follows: 1. The so-called cardiac glands have a number of parietal cells and cells distributed in the same glandular base contain fine pepsinogen granules. However, these glandular cells are filled with a PAS-positive substance. Accordingly, the so-called cardiac glands are nothing but undifferentiated gastric glands. 2. Due to the deep gastric pits, the outer layer of the mucous membrane in the clear zone is loose, and a distribution of a smaller number of blood vessels can be expected. These facts may demonstrate a pale or clear appearance of the mucous membrane in the same zone. 3. The gastric pits in the dark zone are extremely shallow. Accordingly, the mucous membrane in this area is dense and a distribution of a large number of blood vessels can be expected. This probably produces a dark color, i.e., reddish brown. 4. The chief cells in the glandular base of the clear zone contain a number of fine and coarse PAS-positive granules. These granules also are a violet color with PAS-AB (pH 2.5) stain. This color resulted from sialomucin. The above mentioned facts mean that the clear zone is an undifferentiated region of the gastric glands. 5. The chief cells distributed in the glandular body and base of the dark zone are PAS-negative and contain no sialomucin. Accordingly, the same zone is the differentiated region of the gastric glands.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

Do the cardiac glands exist? 6. The cat.

We performed histological and histochemical investigations on so-called cardiac glands and gastric glands in the cat to clarify the existence of the former. 1. Cardiac glands possess some parietal cells and strongly PAS-positive glandular cells in the base of glands containing a small number of fine pepsinogen granules. Consequently, we consider them to be undifferentiated gastric glands. Therefore, the cardiac glands do not exist in the cat. 2. Glandular cells in the basal part of undifferentiated gastric glands react negatively or very weakly to AB (pH 2.5 and 0.5) and present a red color with PAS-AB (pH 2.5) stain. 3. A large number of strongly PAS-positive and pepsinogen granule containing cells are distributed not only in the glandular body but also in the basal part of the glands. These cells are immature chief cells. 4. The above-mentioned cells react weakly or moderately to AB (pH 2.5 and 0.5) and stain light purple by PAS-AB (pH 2.5). They possess weak and strong acid mucopolysaccharides and sialomucin. 5. Mature chief cells are distributed mainly in the glandular base. They are PAS-negative, react moderately or strongly to AB (pH 2.5 and 0.5), and present a dark color of AB with PAS-AB (pH 2.5) stain. Consequently, it can be concluded that these cells contain weak and strong acid mucopolysaccharides but no sialomucin. 6. Mature chief cells possess no sialomucin. However, they present stronger reactions to AB (pH 2.5 and 0.5) than those in immature chief cells. This characteristic does not appear in man and many other types of mammals studied.

Animals↗
PubMed1990

Fluid-path in the mandible and maxilla.

We examined fluid-paths in the maxilla and mandible of the dog, and compared them with Kihara's extravascular fluid-path-system. Areolar tissue is formed in the walls of the Volkmann's and Haversian canals. This tissue is composed of collagenous and reticular fibers. Kihara confirmed the collagenous fibers were encircled, however we could not ascertain whether they were encircled or not. We have compared the paths in the walls of the Volkmann's and Haversian canals with Kihara's fluid paths, and found the paths in the bone do not belong to the category of Kihara's paths. Namely, the term "extravascular fluid path" should not be applied to the areolar tissue in the bone. We suggest the term "fluid path" for this tissue in the bone. The areolar tissue in the walls of the Volkmann's and Haversian canals and endosteum communicate with one another. We call them collectively endosteum. Yoshikawa called the areolar tissue in the Volkmann's and Haversian canals perivasculäre lymsphräume. However, this designation is inadequate considering the actual characteristics of this tissue.

Animals↗
PubMed1990

Comparative histology and histochemistry of the gastric glands of cats and dogs.

We performed histological and histochemical investigations on the gastric glands of cats and dogs. So-called cardiac glands in the cat and dog have a small number of the parietal cells and the PAS-positive glandular cells in the base of these glands contain fine pepsinogen granules. Consequently, we consider them as undifferentiated gastric glands, and can find no differences between these glands in both animals. The immature chief cells in the cat gastric glands are distributed not only in the glandular body but also in the base of the glands and contain sialomucin, and weak and strong acid mucopolysaccharides. The mature chief cells in cat and dog gastric glands contain weak and strong acid mucopolysaccharides. The gastric gland region of the dog is divided into clear and dark zones, and structural differences between the two zones are recognizable. Namely, the chief cells in the glandular base in the clear zone are somewhat undifferentiated, while the chief cells in the middle and lower parts of the glandular body and those in the glandular base in the dark zone are perfectly differentiated. The structure of the gastric glands in the fundic and greater and lesser curvature regions in the cat is very similar.

Animals↗
PubMed1990

Do the cardiac glands exist? 7. The cow.

The glands distributed in the narrow region of the abomasum contiguous to the omasum of the cow have been described as cardiac glands. We doubted this assertion and therefore performed histological and histochemical investigations of the glands to clarify their characteristics. 1. All glandular cells except the parietal cells in a few glands contiguous to the omasum react strongly to PAS, AB(pH 2.5), and PAS-AB(pH 2.5) staining, and moderately to AB(pH 0.5) staining. 2. Glandular cells at the base of these glands contain fine pepsinogen granules and a few parietal cells are distributed in these glands, indicating that they are undifferentiated gastric glands and that the so-called cardiac glands do not exist in the cow stomach. 3. Glandular cells in undifferentiated gastric glands are filled with PAS, AB(pH 2.5 and 0.5) and PAS-AB(pH 2.5) positive substances. Which gradually decrease and finally disappear with differentiation, remaining only in the neck (mucous neck cells) and the cells in the upper part of the glandular body (immature chief cells), in mature gastric glands. 4. Mature chief cells in differentiated gastric glands are distributed in the middle and lower bodies and base of the glands and contain a number of PAS and PAS-AB(pH 2.5) positive granules and a large number of coarse pepsinogen granules, while pepsinogen granules in the mucous neck cells and immature chief cells are finer. 5. In the cow the region in which undifferentiated gastric glands are located is very narrow. 6. Parietal cells in the cow stomach are numerous.(ABSTRACT TRUNCATED AT 250 WORDS)

Abomasum↗
PubMed1989

Stimulated intraphagosomal release of eosinophil peroxidase and acid phosphatase in the rats spontaneously infected with Mycoplasma pulmonis: a cytochemical study.

In this study we performed a cytochemical comparison of peroxidase and acid phosphatase activities in peritoneal eosinophils from specific pathogen-free (SPF) and Mycoplasma pulmonis-infected rats. When eosinophils ingested polystyrene particles for 120 min, peroxidase- and acid phosphatase-positive specific granules, as well as small granules, fused to phagosomes. Unusual peroxidase activity was detected in some particle-containing phagosomes in 6.8% of eosinophils from control SPF rats and 31% of those from infected rats. Intense acid phosphatase activity was also demonstrated in some phagosomes in 4 and 20% of eosinophils from control and infected rats, respectively. The rate of peroxidase-positive and acid phosphatase-positive phagosomes to all ingested phagosomes was 8.1 and 7.3% in control rats and rose to 41.3 and 31.1% in those infected, respectively. The population of eosinophils (18%) in the control peritoneal cells did not change after infection (16.6%). These results suggest that the intraphagosomal release of lysosomal enzymes was significantly stimulated in peritoneal eosinophils of the rats spontaneously infected with M. pulmonis.

Acid Phosphatase↗
PubMed1989

[Morphological studies on the margo infraorbitalis (on the longitudinal changes of infraorbital suture)].

The purpose of this study was to obtain information on the developmental changes of the infraorbital margin and the form of infraorbital suture. 136 Indian craniums were divided into five developmental groups according to the stage of eruption of the teeth. The anterior view of the skull was observed photographically. The results are summarized as follows: 1. The infraorbital margins were divided into three types according to the shape of the infraorbital suture. Type I: Infraorbital suture was independent. Type II: Infraorbital suture and zygomatico-facial suture coincided. Type III: Infraorbital suture and zygomatico-facial suture were joined. Type I was the basic type of infraorbital suture. 2. The frequency of type I was 90% at the pre-eruption stage, 31.8% at the eruption stage, 41.7% at the deciduous dentition stage, 56.3% at the mixed dentition stage, and 62% at the permanent dentition stage. 3. On the distance between the median line and each point of the infraorbital margin the growth rate is expressed in values relative to a standard that was based on the pre-eruption stage. The growth rate was greatest at the highest point of the superior margin in the infraorbital foramen (1.79 times), the middle rate was at the position of the infraorbital suture (1.72 times) and the smallest rate was 1.63 times at the position of the zygomatico-facial suture. Each measuring point moved towards lateral side.

Cephalometry↗
PubMed1989

Does the polystomatic gland exist?

According to the P.N.A., the N.A.J. and some scholars, the sublingual gland has the ductus sublingualis major and ductus sublinguales minores. This means that the gland is a polystomatic gland. We intended to determine whether the so-called polystomatic gland exists or not. 1. According to the P.N.A., the N.A.J. and some scholars, the gl. sublingualis has the ductus sublingualis major and ductus sublinguales minores. This means the gland is a polystomatic gland. However, the formation of one gland with plural excretory ducts is embryologically impossible, in other words, the polystomatic gland does not exist. 2. Many scholars described that the gl. sublingualis was composed of the gl. sublingualis major and g11. sublinguales minores. However, they are completely different kinds of glands. Accordingly, we suggest the terms for these glands: the g1. sublingualis and its ductus sublingualis ("major" is useless), the g11. sublinguales minores and their ductus sublinguales minores. 3. The N.A.V.J. and some scholars use the term g1. sublingualis polystomatica or parvicanalaris. However, this is a group of a number of independent glands each of which has its own excretory duct. Such a gland should not be regarded as a single gland. We suggest that the term g11. sublinguales minores and their excretory ducts should be replaced with the term the ductus sublinguales minores. 4. The g1. lingualis anterior, g1. retromolaris and g1. lacrimalis are not single glands but a group of several independent glands each of which has its own excretory duct. Accordingly, they should be termed the g11. linguales anteriores, g11. retromolares and g11. lacrimales such as the g11. labiales, g11. buccales and g11. palatinae.

Animals↗
PubMed1989

A new differentiation type of the chief cells in the cat gastric gland.

We demonstrated a new differentiation type of chief cell in the cat gastric gland. 1. Numerous PAS-positive cells were distributed not only in the glandular body but also in the glandular base. 2. These cells, namely immature chief cells, contained a large number of claret-colored fine pepsinogen granules. 3. Immature chief cells did not exist in the glandular base of man and many other types of animals. 4. The PAS-positive cells in the glandular base gradually decreased in PAS-positive substance and changed into PAS-negative cells with a reticulate framework. These mature chief cells contained numerous dark blue coarse pepsinogen granules. 5. The mature chief cells reacted moderately to AB (pH 2.5), and weakly to AB (pH 0.5), while staining with PAS-AB (pH 2.5) produced a moderate to strong color reaction of AB. Such reactions were characteristic to these cells and could not be found in man or many other types of animals studied. 6. PAS-positive cells i.e. the immature chief cells, reacted strongly to AB (pH 2.5), and moderately to AB (pH 0.5), while staining with PAS-AB (pH 2.5) produced a violet color. 7. The above-mentioned reactions were the same in mucous neck cells with mean undifferentiated characteristics. Such cells do not exist in the glandular base of man and many other types of animals. 8. The glandular base is generally occupied only by mature chief cells, though a large number of undifferentiated cells or immature chief cells were distributed in the same area of the cat.(ABSTRACT TRUNCATED AT 250 WORDS)

Aminosalicylic Acid↗
PubMed1988

S-adenosyl-L-methionine protects the hippocampal CA1 neurons from the ischemic neuronal death in rat.

We investigated the effect of S-adenosyl-L-methionine (SAMe) on the prevention of the delayed neuronal death in rats subjected to transient and brief forebrain ischemia. As the results, SAMe dose-dependently protected the hippocampal CA1 neurons from degeneration and necrosis, whose effect was suppressed by simultaneous administration of S-adenosyl-L-homocysteine, a potent inhibitor in transmethylation. No protective effect was observed in CDP-choline, phosphatidylcholine and L-methionine. Therefore, it is necessary for the prevention of the delayed neuronal death to enhance cerebral SAMe level and to activate transmethylation using SAMe as a methyl donor in postischemic brain.

Animals↗