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Biomedical subjects

K Miyazono

Publications and source records attributed to K Miyazono.

248 records · Page 14Linked to original sources
PubMed1988

[Treatment with degradable starch microspheres (DSM) in malignant hepatic tumors-clinical experience of implantable drug delivery system (Port-A-Cath)].

Chemoembolization with degradable starch microspheres (DSM) was performed in twelve cases of hepatocellular carcinoma, one case of cholangioma, and one case of metastatic liver cancer. The following results were obtained. (1) Tumor regression of over 50% was observed in eight of fourteen cases. Tumor regression of over 25% was obtained in ten of fourteen cases. (2) Half of the cases had fever, pain and leukocytopenia, but they were slight and transient. (3) Implantable drug delivery system (Port-A-Cath) was applied for intra-arterial chemoembolization in four cases. The longest implantation period was 662 days and 33 infusions were made. These results suggest that chemoembolization with DSM can be effectively used in the treatment of malignant hepatic tumors.

Adenoma, Bile Duct↗
PubMed1987

Purification and properties of an endothelial cell growth factor from human platelets.

An endothelial cell growth factor has been purified about 1,000,000-fold to homogeneity from human platelets by a seven-step procedure. The purified product has an apparent Mr on sodium dodecyl sulfate-polyacrylamide gels of 45,000. The mobility in sodium dodecyl sulfate gel electrophoresis was similar in the presence or absence of reducing agents, indicating that the factor consists of a single polypeptide chain. Maximal stimulation by the purified protein was achieved at a concentration of about 20 ng/ml (440 pM). Heparin did not potentiate the activity, nor did the factor bind to heparin immobilized on Sepharose. The purified factor was heat- and acid-labile; it was active on porcine and human endothelial cells, but not on human foreskin fibroblasts. Chromatofocusing revealed that the pI of the factor was 4.6. The structural and functional characteristics of the platelet-derived endothelial cell growth factor are distinct from previously characterized endothelial cell mitogens with affinities for heparin.

Animals↗
PubMed1985

A platelet factor that stimulates the proliferation of vascular endothelial cells.

The effects of platelet factors on the growth of cultured porcine aortic endothelial cells were studied. Human platelet lysate stimulated the incorporation of [3H] thymidine into DNA. Gel chromatography on Sephadex G-75 revealed at least two peaks of activity on endothelial cells, the major peak being at an apparent molecular weight of 20,000. This activity was heat-labile and trypsin-sensitive, and did not stimulate the growth of fibro-blasts.

Animals↗
PubMed1985

A platelet factor stimulating the proliferation of vascular endothelial cells. Partial purification and characterization.

Platelets have been shown to contain a novel growth factor that stimulates the proliferation of vascular endothelial cells in vitro. The factor potently stimulated both DNA synthesis and proliferation rate in serum-deprived endothelial cells. Gel exclusion chromatography showed at least two peaks of activity on endothelial cells, the major peak being at an apparent molecular weight of 20 000. Isoelectric focusing revealed that the pI of the factor was 4.0-4.8. It was adsorbed to a column of DEAE ion exchange chromatography and eluted with a salt gradient. The factor was heat-labile and trypsin-sensitive. The activity was not destroyed by a reducing agent including dithiothreitol. This factor stimulated the proliferation of vascular endothelial cells but was found to be inactive against normal rat kidney fibroblasts.

Animals↗
PubMed1984

Propylthiouracil-induced diffuse interstitial pneumonitis.

Cough productive of sputum, exertional dyspnea, and hypoxemia developed in two patients with Graves' disease after six months (patient 1) or three weeks (patient 2) of treatment with propylthiouracil, 300 mg/day. Chest roentgenograms and transbronchial lung biopsy specimens revealed diffuse interstitial pneumonitis. Lymphocyte transformation by phytohemagglutinin was highly stimulated by propylthiouracil. Symptoms and signs improved after cessation of the drug therapy and administration of prednisolone acetate. These cases represent the first report of a complication of diffuse interstitial pneumonitis induced by propylthiouracil.

Aged↗
PubMedDate not supplied

TGF-beta signaling by Smad proteins.

Smads are signal transducers for the members of the transforming growth factor-beta (TGF-beta) superfamily. Bone morphogenetic proteins (BMPs) and their receptors induce differentiation of C2C12 cells into osteoblast-like cells. Using an adenoviral expression vector system, we showed that receptor-regulated Smads (R-Smads) activated by BMPs can induce the differentiation of C2C12 cells. Inhibitory Smads (I-Smads) interfere with the osteoblast differentiation of C2C12 cells by preventing the nuclear translocation of R-Smads. After translocation into the nucleus, Smad oligomers regulate the transcription of target genes through binding to DNA directly, interaction with other DNA binding proteins, and recruitment of transcriptional co-activators or co-repressors. Through interaction with different transcription factors and transcriptional co-activators or co-repressors, Smads may exhibit specific effects in various cell types.

Adenoviridae↗
PubMedDate not supplied

Distribution of human granulocyte colony-stimulating factor receptors on hematopoietic and nonhematopoietic tumor cell lines.

Introduction of two tyrosine residues into human granulocyte colony-stimulating factor (G-CSF) allowed us to obtain radioiodinated material efficiently. By using this material, specific receptors for human G-CSF were analyzed. It was found that human G-CSF receptors were expressed on several myelocytic and monocytic leukemia cell lines. Furthermore, they were also expressed on two choriocarcinoma cell lines. However, the human G-CSF receptors were not detected on other nonhematopoietic tumor cell lines examined, indicating that expression of G-CSF receptors is strictly limited as compared with receptors for granulocyte/macrophage colony-stimulating factor (GM-CSF), which are widely distributed on nonhematopoietic tumors.

Cell Line↗
PubMedDate not supplied

Treatment of infective endocarditis with granulocyte colony-stimulating factor.

Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein hormone which stimulates the proliferation and differentiation of a subset of granulocyte precursors and enhances some functional activities of mature neutrophils. We tested the effects of G-CSF on a patient with intractable infective endocarditis. The white blood cell count was increased 3-fold and the inflammatory reactions such as C-reactive protein and erythrocyte sedimentation rate were completely normalized without any side effects. This is the first report describing the use of G-CSF for infective endocarditis. Administration of G-CSF might be suitable for treating intractable infections which cannot be controlled by antibiotics alone.

Aged↗