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Biomedical subjects

K Miyazawa

Publications and source records attributed to K Miyazawa.

At least 235 records · Page 13Linked to original sources

Laparoscopy and minilaparotomy as operative management of ectopic pregnancy.

The widespread use of serum human chorionic gonadotropin (hCG) monitoring has enabled earlier diagnosis of ectopic pregnancy and increasingly conservative methods of management including operative laparoscopy, medical therapy with methotrexate, and expectant management. We investigated the use of a combined laparoscopic and minilaparotomy approach as an operative management of ectopic pregnancy. Fifteen of 16 patients presenting to the Reproductive Endocrinology Service were managed using either operative laparoscopy or minilaparotomy. Serum hCG values at the time of diagnosis ranged from 239 to 8060 mIU/ml. Nine patients were managed by operative laparoscopy and six by minilaparotomy. All patients were ambulatory and tolerating oral intake within 8 hours of surgery. The average hospitalization was 31 hours from the time of surgery. Thirty-five percent of the patients were discharged within 24 hours and an additional 40% within 36 hours. This approach was applicable to all patients regardless of body habitus, degree of hemoperitoneum, or status of the ectopic pregnancy. Such a combined approach permitted minimal tissue manipulation and shortened hospitalization. Data of the present study suggest that such an approach may be applicable to most clinical circumstances, performed without undue morbidity and potentially avoid a standard laparotomy and its attendant risks and hospitalization.

Chorionic Gonadotropin↗

Beneficial effects of a new 5-lipoxygenase inhibitor on occlusion- and occlusion-reperfusion-induced myocardial injury.

Effects of a new 5-lipoxygenase inhibitor TZI-41127 (2-(4-hydroxy-3,5-dimethylphenyl)-5-methoxy-3-methylindole) on occlusion- and occlusion-reperfusion-induced myocardial injury were examined in rats and dogs. The occlusion-reperfusion-induced myocardial injury was produced by the occlusion of the left anterior descending (LAD) coronary artery for 90 min following 22 h reperfusion in Beagle dogs. The drug was orally administered twice in a dose of 30 mg/kg. The first administration was 2 h before the occlusion and the second one was 1 h after the start of the occlusion. Eighteen dogs were divided into two groups. Nine were for control which were administered the vehicle, and the other were for the drug treatment. One animal of each group died because of ventricular fibrillation. The risk area and the infarct area were measured 22 h after reperfusion. The risk area as percent of total ventricle was not different between the two groups. However, the infarct area as percent of the risk area was significantly less in the drug-treated group. Although the ventricular arrhythmias immediately after the reperfusion were not prevented by the drug treatment, the ventricular arrhythmias 22 h after the reperfusion were significantly less in the drug-treated group. The arterial blood pressure, the heart rate and the double product were not significantly different between the two groups. The infarct size induced by LAD occlusion in rats was also reduced by the pretreatment with TZI-41127. Thus, TZI-41127 has beneficial effects on occlusion- and occlusion-reperfusion-induced myocardial injury.

Animals↗

Cervical conization findings in women with dysplastic cervical cytology and normal colposcopy.

A review of 134 cervical conizations performed in a 36-month period showed that 18 were performed on patients with two or more cervical smears showing dysplasia but with essentially negative, satisfactory colposcopic evaluations, including normal colposcopically directed biopsies and endocervical curettings. A histologic diagnosis of cervical intraepithelial neoplasia (CIN) of various grades was established in 15 (83%) of the 18 conization specimens. Six (33%) of the 18 specimens harbored CIN 3. The histologic diagnosis of a more severe lesion than that indicated on cytology was rendered in five of the cases. Cervical conization should be performed when colposcopic evaluation of the cervix, vagina and vulva fails to identify the source of persistent CIN on Papanicolaou smears.

Adult↗

Intermittent ovarian failure. A report of two cases.

Premature ovarian failure, diagnosed from elevated serum gonadotropin concentrations, is generally considered irreversible. That there have been isolated cases of pregnancy, however, suggests that ovarian function may persist in these patients. We recently studied the longitudinal progress of two patients with intermittent ovarian failure who manifested a spontaneous return of ovarian function and, in one patient, pregnancy.

Adult↗

[Mechanism of hyperferritinemia in a case of malignant histiocytosis].

We report a case of malignant histiocytosis diagnosed by liver-spleen biopsy under laparoscopy. A 49-year-old woman was admitted to our hospital with thrombocytopenia, moderate anemia and hypoproteinemia. Her bone marrow findings revealed erythroid and megakaryocyte hyperplasia, and the serum ferritin concentration was 2,250 ng/ml though she had not received any blood transfusions. Ferrokinetics analysis showed the pattern of ineffective erythropoiesis, and the half-lives of erythrocytes and platelets were both shortened. Her hepatosplenomegaly gradually increased accompanied by increasing serum ferritin level to 10,000 ng/ml. Liver-spleen biopsy was carried out under laparoscopy and revealed infiltration of atypical histiocytes with erythrophagocytosis, which were positive for S-100 and ferritin but negative for lysozyme. The rate of glycosylation in whole serum ferritin, analyzed by using concanavalin-A binding method, showed that her glycosylated ferritin content was only 8.3%, whereas in sera after iron overloading, it was about 70%. Serum isoferritin profiles by isoelectric focussing were studied, and isoferritin pattern from malignant histiocytosis was the same as that in iron overloading after neuraminidase treatment. These findings suggest that serum ferritin is synthesized in proliferating histiocytes and released in the plasma as a nonsecretory type (nonglycosylated ferritin) in this case.

Female↗

[Immunoelectron microscopic studies on the localization of creatine kinase M in normal and ischemic myocardial cells].

The localization of creatine kinase M (CK-M) in both normal and acute ischemic canine myocardial cells was studied by immunoelectron microscopy using the anti-CK-M Fab'-horseradish peroxidase conjugate. Myocardial ischemia was induced by occlusion of the left anterior descending coronary artery for 15, 60, or 180 minutes. In the normal myocardial cells, CK-M was localized mostly in the A-band and some in the Z-line, M-line, sarcolemmal membrane, and membrane of sarcoplasmic reticulum. Most CK-M in the A-band appeared to associate with thick fibers. This finding strongly suggests that the CK associated with thick fibers may be the enzyme to rephosphorylate ADP produced by myosin ATPase. In 15 minutes of myocardial ischemia, CK-M showed only minimal changes in its location, i.e., almost similar to normal, indicating that the CK in the A-band still has the ability to couple with myosin ATPase. However, in 60 and 180 minutes of ischemia, the A-band CK dissociated markedly from thick fibers, diffused to the I-band and leaked out to the intercellular spaces. These results suggest that the dissociation and disappearance of the A-band CK from thick fibers induced by progress of myocardial ischemia disrupt the myocardial energy transport system via CK reaction, and lead to the irreversible injury of myocardial cells.

Adenosine Triphosphate↗

Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor.

Amino acid sequences of four peptide fragments of human hepatocyte growth factor purified from the plasma of patients with fulminant hepatic failure were determined. Based on the amino acid sequence of one of the fragments, two oligodeoxyribonucleotide mixtures were synthesized and used to screen a human placenta cDNA library. On the screening, two overlapping cDNA clones for human hepatocyte growth factor were isolated and the nucleotide sequence of the cDNA was determined. The entire primary structure of the protein was deduced from the sequence. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. The sequence revealed that the heavy and light chains which comprise the protein are encoded by the same mRNA and are produced from a common translation product by proteolytic processing.

Amino Acid Sequence↗

Electrophysiological abnormalities and enhanced reperfusion arrhythmias in the isolated hearts of hyperthyroid rats.

1. The influence of hyperthyroidism on electrophysiological characteristics and on reperfusion arrhythmias was examined in rat hearts. 2. Electrophysiological studies were performed with glass microelectrodes, and the experiments on reperfusion arrhythmias were done in isolated perfused hearts. 3. Ventricular muscle from hyperthyroid rats was more prone than that from euthyroid rats to develop triggered activity under conditions believed to cause myoplasmic Ca2+ overload. 4. The severity of reperfusion arrhythmias was significantly enhanced in hyperthyroid preparations as compared with euthyroid ones. 5. The enhanced reperfusion arrhythmias in hyperthyroid rats were significantly reduced by propranolol (3 x 10(-7) M), lignocaine (1 x 10(-5) M) and verapamil (3 x 10(-8) M), but not by nadolol (3 x 10(-7) M) or prazosin (3 x 10(-7) M). 6. These results suggest that increased heart rate due to hyperthyroidism and responses mediated via either alpha- or beta-adrenoceptors were not dominant causes of enhanced reperfusion arrhythmias in hyperthyroid hearts. 7. The increased tendency to develop triggered activity which was observed in the electrophysiological study, may be one possible explanation of enhanced reperfusion arrhythmias in hyperthyroid hearts.

Action Potentials↗

Immunocytochemical localization of creatine kinase M in canine myocardial cells: most creatine kinase M is distributed in the A-band.

The localization of creatine kinase (CK) M in canine myocardium was immunocytochemically studied by a direct immunoperoxidase method. Specific antiserum against CK-M was produced in rabbits immunized with canine CK-MM. An anti-CK-M Fab'-horseradish peroxidase conjugate was prepared by the maleimide method. Frozen sections prepared from fixed canine myocardium were stained with the conjugate and observed by light and electron microscopy. In light microscopy of longitudinal sections, CK-M showed a cross-striated pattern consisting of distinct broad and narrow brown bands. Immunoelectron microscopy revealed that the regions of the broad and narrow brown bands corresponded to the A-band and the Z-line, respectively. Most CK-M in the A-band was associated with the thick fibers, and a small amount of CK-M was found in the M-line. These findings suggest that ATP regeneration from the ADP produced by myosin ATPase is related to the participation of this CK associated with the thick fibers rather than that of the M-line-bound CK. Creatine kinase M was also found in the sarcolemmal membrane, the membranes of the sarcoplasmic reticulum, and the mitochondrial outer and inner membranes. This report provides new information for understanding the physiological role of the phosphorylcreatine shuttle in the myocardial energy transport system.

Animals↗

Immunological investigation of intestinal, liver, kidney, bone, placental and serum alkaline phosphatase in cattle.

The purpose of this study is to investigate the immunological difference between intestinal, liver, kidney, bone, placental and serum alkaline phosphatase (ALPs) in cattle. Kidney, bone and placental ALPs were purified from each tissue by gel filtration and ion-exchange chromatographies, and intestinal, liver, and placental ALPs were obtained from a commercial source. The antibody to each tissue ALP was prepared by immunizing rabbits and tested by double immunodiffusion analysis in cross reactivities. The results indicated that the bovine tissue ALPs are divided into four groups in antigenicity, that is, intestinal, bone, liver and kidney-placental ALPs. The fetus, calf and cow sera contained bone ALP, and calf and cow sera also contained kidney or placental ALP, but all of the sera did not contain intestinal and liver ALP.

Alkaline Phosphatase↗

Cardiovascular response to exercise in pacemaker implanted patients with fixed heart rate.

Cardiovascular response to supine exercise was studied in 11 patients with chronic ventricular pacing for complete atrioventricular block whose heart rate was fixed during exercise, and compared with 11 age- and sex-matched normal subjects. Oxygen uptake increased linearly with increasing work rate, and attained maximum values of 15.7 +/- 0.7 and 29.5 +/- 1.1 ml/min.kg, respectively. In both groups, cardiac output increased in association with oxygen uptake, although the maximum value in the patients was less than one half that in the controls (6.8 +/- 0.5 and 15.1 +/- 0.3 l/min). Stroke volume, however, could change in patients, as in the controls under similarly increased afterloads (maximum values were 96 +/- 7 and 93 +/- 3 ml/beat, respectively). During exercise, the systolic arterial pressure elevated to a similar extent in both groups (221 +/- 14 and 235 +/- 12 mmHg, respectively). The increase in stroke volume in the patients was achieved by complete systolic emptying and the Frank-Starling mechanism. It is considered that in patients with ventricular pacing, exercise can produce an increase in stroke volume when myocardial function is not impaired.

Aged↗

[Inhibitory effect of sodium pentosan polysulfate on the formation, growth and aggregation of calcium oxalate in vitro].

The inhibitory effects of sodium pentosan polysulfate (SPP) on the formation, growth and aggregation of calcium oxalate (CaOx) crystals were estimated by means of a Coulter counter TA II by the seed crystal method, whole urine method and infrared spectroscopic method. In the seeded crystal system, the inhibitory activities on aggregation (Ia), growth (Ig) and size (Is) were calculated from the change of the number, volume and diameter of crystals. At above 0.5 microgram/ml, SPP inhibited the aggregation and growth of added CaOx monohydrate (COM) and dihydrate (COD) crystals. In the whole urine system, after determining the metastable limit, the formation and growth of CaOx crystals precipitated in response to a load of sodium oxalate was measured. SPP had a strong inhibitory effect on the formation and growth in the concentration of over 5 micrograms/ml. In infrared spectroscopic study, the ratio of COD/COM formed in whole urine increased with the concentration of SPP. SPP therefore might have prevented the recurrence of stone formation.

Agglutination↗

[The quantitative study of inhibitory effect of pentosan polysulfate and chlorophyllin on the experimental calcium oxalate stone].

The purpose of this study is to evaluate the effect of sodium pentosan polysulfate (SPP) and sodium copper chlorophyllin (SCC) on the formation, growth and aggregation of calcium oxalate crystals in vivo, and to measure the number and the volume of crystals formed in the rat kidney, quantitatively, with a Coulter counter TA-II. The deposition of calcium oxalate crystals in the rat kidney was induced by intraperitoneal injection of 2.5 g per Kg of body weight of hydroxy-L-proline and administration of 0.4% ethylene glycol as the drinking fluid ad libitum for 7 days. Daily excretions of urinary oxalate, calcium (ratio to urinary creatinine) and urinary volume were measured. Both kidneys were removed after protocol. The kidneys were homogenized with 0.2 M Tris-buffer (pH 8.0) and subsequently digested in soluene-100. After calcium oxalate crystals were collected, they were suspended in saline saturated with calcium oxalate. The crystal size distribution was measured with a Coulter counter TA-II. In addition, the renal calcium content was measured by atomic absorption spectrometry, and the kidneys were examined by optical microscopy and scanning electron microscopy. The crystals formed in the rats' kidneys were analyzed by infrared spectroscopy. The results were as follows: 1. There was no deposition of crystals in the kidney of the rats which were not treated. There was intratubular deposition of crystals in the kidneys of the rats injected with hydroxy-L-proline and administered 0.4% ethylene glycol. They consisted of calcium oxalate monohydrate. 2. Renal calcium content was significantly higher in the groups with induced crystals than the control group.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

[Prophylaxis of recurrence of superficial bladder tumor by intravesical chemotherapy].

During the past 10 years, we have experienced 110 bladder tumor cases. Among them, 70 patients were diagnosed superficial bladder tumor. Of these 70 cases, 30 were treated with intravesical adriamycin (ADR) and peplomycin (PEP), 13 with ADR only and one case with PEP and remaining 26 with TUR and hydrostatic pressure technique. We studied the efficacy of combination intravesical chemotherapy with ADR and PEP and other treatments in the prevention of recurrence in the superficial bladder tumor cases. The recurrence rate during 3 years of each group, was 25% in the group treated with ADR and PEP, 35% with ADR and 55% in remaining group. 3 years recurrence rate in the group treated with ADR and PEP was significantly low than that in the group tread with TUR and hydrostatic pressure technique alone (Wilcoxon test). Side effects was pollakisuria, pain after micturition and others. Anaphylactic shock appeared in one case. From these results we concluded that intravesical chemotherapy with combined agents is more effective than that with a single one or no treatment after TUR.

Administration, Intravesical↗

Evaluation of the direct trocar insertion technique at laparoscopy.

A technique using direct trocar insertion for laparoscopy was introduced into the Tripler Army Medical Center Obstetrics and Gynecology training program in September 1982. From January 1, 1983 to December 31, 1985, a total of 1249 laparoscopic procedures were performed. Detailed records of 937 laparoscopic procedures performed using this technique were reviewed. The technique was used with increasing frequency: 40% in 1983, 85% in 1984, and 96% of all laparoscopies in 1985. There were no major complications. With increased departmental experience, the frequency of minor complications decreased: 5.3% in 1983, 5.0% in 1984, and 1.3% in 1985. Obesity was associated with a significantly increased risk of minor complications (P less than .001). A history of abdominal surgery was not associated with an increased risk of minor complications. Surgical skill level was not associated with the complication rate.

Cervix Uteri↗