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Biomedical subjects

K Miyata

Publications and source records attributed to K Miyata.

At least 343 records · Page 19Linked to original sources
PubMed1987

Effects of presensitization of antiheart serum on the rat embryonic heart.

After sensitization by rabbit anti-rat-heart serum (AHS), Wistar rats were injected with 9 ml/kg of AHS on day 9 of gestation. The incidence of malformations was 31.5% and that of cardiovascular malformations was 21.8%. The main malformations were microphthalmia, ventricular septal defect, pulmonary stenosis, and general edema. No antinuclear antibody was detected in the maternal sera by the fluorescent antibody technique. No complete heart block was found in the fetuses by electrocardiography. The fluorescent antibody technique demonstrated localization of rat IgG and rat C3 on the maternal myocardium, but a light-microscopic study revealed no myocarditis or endocarditis in either the maternal or the fetal heart. The types of malformation were similar to those observed after a single administration of antikidney serum, which presumably involves yolk sac dysfunction. These results show that both the existence of the immune complexes of antibodies against AHS and the administration of AHS on day 9 are necessary to cause yolk sac dysfunction or other teratogenic changes.

Abnormalities, Multiple↗
PubMed1987

Comparative studies of the dorsal surface of the tongue in three mammalian species by scanning electron microscopy.

Comparative features of the dorsal tongue epithelia in musk shrews, mongooses and rats were described. The shapes of the filiform papillae were different in each of the species. The distribution pattern of filiform papillae was similar both in the musk shrews and mongoose, in that the form of filiform papillae changed gradually from the lingual apex to the posterior part of the lingual body. By contrast, the different types of filiform papillae were distributed on definite areas of the dorsal lingual surface in the rat. Microridges on the interpapillar surface in the musk shrew and mongoose presented a clear outline, but those of the rat were not so distinct. In all species, the upper surface of filiform papillae did not show any distinct microridges.

Animals↗
PubMed1987

Three-dimensional structures of the connective tissue papillae of the tongue in newborn dogs.

Connective tissue papillae underlying epithelial cell layers of the lingual papillae of newborn mongrel dogs were exposed by long term hydrochloric acid treatment and observed by scanning electron microscopy. Each of the filiform papillae of ovoid shape (100-130 microns in diameter) contained a connective tissue papilla representing a smaller elliptical protrusion (90-100 microns in length, 70-80 microns in width). Its overview showed a horse-shoe shaped, opening anteriorly. Its posterior curvature projected a conical node with a rounded apex. Each of the fungiform papillae (150 microns in diameter) contained a connective tissue papilla of rounded shape (100 microns in diameter) whose upper surface was facetted with several slight depressions. The entire surface of the connective tissue papillae was found to be covered by networks of collagenous fibers of varying thickness, running in various directions. The basal surface of the epithelium revealed regularly distributed round holes that corresponded to the connective tissue papillae. In the filiform papillae, a small elliptical bulge was seen on the anterior side of each hole. Light microscope observation indicated that the anterior column of the filiform papillae contained a granular layer with moderate keratohyaline granules, whereas the posterior column did not show such a granular layer.

Animals↗
PubMed1987

[Alternation of the cellular immunity of patients with head and neck carcinomas under chemotherapy].

In 20 cases of head and neck carcinomas, we have studied the immunological alternation by combined chemotherapy with Cisplatin, Vincristine, and Peplomycin (COP therapy). After COP therapy, the number of peripheral lymphocytes decreased, but the OKT4/8 ratio, the PHA-blastogenesis and the positive rate of skin tests increased. The cellular immunity was distinctly augmented by COP therapy. When compared with results from radiation therapy and operation. This augmentation was suspected to have been caused by the suppressive change of the suppressor T cells.

Adult↗
PubMed1986

Purification of natural human interferon-gamma by antibody affinity chromatography: analysis of constituent protein species in the dimers.

A simple procedure for purifying human interferon-gamma from leukocytes was established, based on monoclonal antibody affinity chromatography. The recovery of interferon activity was essentially quantitative, and the specific activity of the product was (4-12) x 10(7) international units/mg protein. SDS-polyacrylamide gel electrophoresis reproducibly revealed four components associated with interferon activity (and no other proteins): two major ones with molecular weights (MW) of 24,000-25,000 (25K) and 19,000-20,000 (20K), a minor one with MW 14,000-15,000 (15K) (these three bands were doublets), and a still less prominent one(s) with MV 40,000-48,000. Gel filtration in neutral solution indicated that all the 25K, 20K, and 15K species exist as oligomers, probably dimers. By means of experiments using a cleavable crosslinking reagent, the dimers were shown to comprise both homo-and heterodimers. Gel filtration in alkali (the condition used during purification) indicated that the molecules are largely in a monomeric state. Thus, the molecules once dissociated in alkali appear to reassociate at random upon neutralization; this process takes place without being accompanied by inactivation.

Amino Acid Sequence↗
PubMed1986

Biochemical properties of beta-lactamase produced by Legionella gormanii.

beta-Lactamase was purified from a strain of Legionella gormanii. The molecular weight of the purified enzyme was 25,000, and its isoelectric point was 10.5. The enzyme hydrolyzed oxyiminocephalosporins, cephamycins, penicillins, and imipenem. The enzyme activity was inhibited by EDTA, Hg2+, and Cu2+, but not by clavulanic acid, sulbactam, or imipenem.

Anti-Bacterial Agents↗