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Biomedical subjects

K Minato

Publications and source records attributed to K Minato.

At least 163 records · Page 9Linked to original sources
PubMed1983

A novel Japanese Burkitt's lymphoma cell line, P32/Ishida, with a new variant chromosomal translocation (2;14).

A novel cultured cell line, P32/Ishida, was established from a Japanese 8-year-old boy with abdominal Burkitt's lymphoma. The P32/Ishida cells were proved to have immature B-cell phenotypes on the basis of immunological surface marker analysis: surface immunoglobulins (gamma, mu, kappa), Ia-like antigen, B1 antigen, and common acute lymphoblastic leukemia antigen were positive. A small amount of IgM, but no IgG, could be detected in both cell extract and culture supernate of P32/Ishida cells. Cytogenetic studies revealed that the P32/Ishida cells consisted of cells having three derivative karyotypes characterized by common marker chromosomes, dup(1), and 14q + due to a new variant chromosomal translocation (2;14), which has not been identified in usual Burkitt's cell lines. Epstein-Barr virus nuclear antigen and terminal deoxynucleotidyl transferase activity were confirmed to be negative.

Antibodies, Monoclonal↗
PubMed1982

Studies with multiple markers on malignant lymphomas and lymphoid leukemias.

Three hundred consecutive cases of malignant lymphomas and lymphoid leukemias in the National Cancer Center Hospital during the last five years were evaluated in terms of multiple morphologic and functional parameters. Immunologically these cases were composed of 42 cases of nonT-nonB-, 135 cases of T-, 95 cases of B-, and 28 cases of possible (defective) B-cell lymphoma and leukemias. The high ratio of T-cell lymphomas (45) is noteworthy and 63% of them were of peripheral T-cell origin. Some of the peripheral T-cell lymphomas were difficult to subtype by the classical Rappaport classification and these were dealt with as separate entities. The lower frequency of follicular lymphoma in Japan was reconfirmed (22 of 95 B-cell lymphomas) and half of the B-cell lymphomas were diffuse large cell type. Morphologic characteristics, surface and other functional markers of the neoplastic cells in each subtype were presented in detail and discussed in relation to the normal differentiation and maturation of lymphoid cells.

Antigens, Neoplasm↗
PubMed1982

Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3.

A panel of monoclonal antibodies (BA-1, BA-2 and BA-3) were made against the pre-B acute lymphoblastic leukemia (ALL) cell line NALM-6. BA-3 binds to the 100,000 mol. wt common ALL antigen (gp100/CALLA), BA-2 binds to a 24,000 mol, wt (p24) cell surface structure and BA-1 binds to a currently undefined antigen. These antibodies were analysed for their reactivity with 67 human leukemia/lymphoma cell lines. The cell lines studied included T ALL, non-T ALL, surface immunoglobulin+ leukemias/lymphomas, and myeloid/monocytoid leukemias. The results indicate that all three antibodies react primarily (but not exclusively) with malignant cells early in B cell development. This pattern of reactivity was similar to previous results obtained with fresh, non-cultured malignant cells. BA-1, BA-2 and BA-3 are useful tools for analysing the developmental options of normal lymphoid cells and for "cleaning up" leukemia/lymphoma cells in selected cases of autologous bone marrow transplantation.

Antibodies, Monoclonal↗
PubMed1982

Clinical evaluation of emission tomography using seven-pinhole collimator: improved detection of perfusion defect by the addition of the right anterior oblique projection.

The clinical efficacy of thallium emission myocardial tomography using a seven-pinhole collimator in the right anterior oblique (RAO) projection was evaluated. Myocardial tomography in left anterior oblique (LAO) and RAO projections was performed following planar thallium perfusion imaging at rest in 11 patients with myocardial infarction. The RAO tomogram was useful, especially in detecting apical perfusion defects, while the LAO tomogram was useful in detecting inferior and anteroseptal wall defects. For the four patients without an apparent myocardial perfusion defect seen on the planar image, the LAO tomogram showed a perfusion defect in three, and the RAO tomogram also showed the defect in three. One or the other of the two views demonstrated the defect for all 11 patients. The myocardial tomogram in the RAO projection, providing a sufficiently high-quality and high-contrast image, gives useful information complementary to the findings of the commonly used LAO projection in the evaluation of myocardial infarctions.

Adult↗
PubMed1982

[Combination chemotherapy of primary gastric lymphoma with vincristine, endoxan (cyclophosphamide), prednisolone and adriamycine (VEPA)].

A new combination chemotherapy(VEPA) was applied to 9 patients with advanced and/or non-resectable primary gastric lymphoma. Among 6 patients with measurable disease, there were 3 (50%) complete responses and 1 (17%) incomplete response. None of 3 patients with lymph node and/or anastomosis involvements at the gastrectomy have relapsed after 13 months to 32 months. Complications during chemotherapy with VEPA were mild myelosuppression and gastrointestinal disturbances, and severe alopecia. Only one paralytic ileus with vincristine occurred, but no cardiotoxicities with adriamycin were observed. This study indicates that VEPA therapy is effective in the patients with primary gastric lymphoma.

Adult↗
PubMed1982

A novel monocytoid cultured cell line, P31/Fujioka, derived from acute monoblastic leukemia.

A novel cultured cell line, P31/Fujioka, of monocytoid nature was established from leukemic cells in the peripheral blood of a seven-year-old boy with acute monoblastic leukemia. The P31/Fujioka cells have abundant cytoplasm, an indented nucleus of monocytoid appearance, pseudopods detectable by electron microscopy and alpha-naphthyl butyrate esterase activity which is completely inhibited by NaF, but they have no peroxidase activity. Immunologically, the P31/Fujioka cells possess Fc gamma-receptor and phagocytic activity towards sensitized erythrocytes (oxEAIgG), and are reactive with various monoclonal antibodies such as OKM1, anti-Mol, FMC10, FMC12 and OKI1. Chromosome analysis revealed the presence of marker chromosome 11q--due to Nos. 7; 11 translocation and No. 9 pericentric inversion. These findings indicate that the P31/Fujioka cells are derived from the patient's monoblastic leukemia cells and show a more distinct monocyte antigen than other known monocytoid cultured cell lines, U-937 and THP-1. The absence of Epstein-Barr virus nuclear antigen of this line was confirmed.

Antibodies, Monoclonal↗
PubMed1982

A B-lymphoma cell line that forms rosettes with neuraminidase-treated sheep erythrocytes through monoclonal surface immunoglobulin.

Undifferentiated lymphoma from a 39-year-old female became serially xenotransplantable to preirradiated nude mice. The tumor cells (KT) possessed a monoclonal surface immunoglobulin (SIg mu, kappa) and formed rosettes with neuraminidase-treated sheep erythrocytes (SEn). Precise characterizations of the SEn rosette, however, revealed the following facts: (1) Neuraminidase-untreated or 2-aminoethylisothiuronium bromide (AET) treated sheep erythrocytes were not bound to the KT cells. (2) SEn rosettes on the KT cells did not show a temperature dependency. (3) Neuraminidase-treated erythrocytes from man, horse, mouse, and rabbit were not bound to the KT cells. (4) Preincubation of the KT cells with antipolyvalent immunoglobulin or anti-kappa-chain serum abolished the SEn rosette formation. (5) Trypsinization decreased both SEn rosettes and SIg on the KT cells. (6) SEn rosettes on the KT cells were too loose to be separated from nonrosetting cells by a Percoll gradient centrifugation method. Summarizing these results, the monoclonal SIg on the KT cells recognized sheep erythrocyte antigen(s) that were exposed only after the neuraminidase treatment. Therefore, this was considered to be a case with peculiar B-lymphoma cells that bound SEn through their SIg.

Adult↗