Biomedical subjects
Publications and source records attributed to K McLeish.
Nursing practice is based on experience, tradition, intuition, common sense, untested theory and sometimes research. There are a number of reasons why nurses may see research as irrelevant to their practice. This paper provides a model identifying possible strategies to help nurses develop research-based practice.
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Receptor-mediated activation of guanine nucleotide binding proteins (G-proteins) is profoundly modified by both mono- and divalent cations. Several divalent cations increase the affinity of many G-protein-coupled receptors for agonists at concentrations in the millimolar range. Micromolar concentrations of Mg2+ are required for receptor-mediated activation of G-proteins. Using the formyl peptide receptor of human leukemia (HL 60) as a model system, we have recently been able to demonstrate that two independent metal binding sites are present on the receptor-G-protein complex to mediate these two effects. We propose that the site that binds divalent cations in the millimolar range is contained in an extracellular domain of the receptor, whereas the site for micromolar Mg2+ is located on the G-protein. Sodium ions decrease the agonist affinity of many G-protein-coupled receptors and modify the interaction of G-proteins with guanine nucleotides. We suggest that sodium ions elicit these effects by interacting with a unique binding site present in an intracellular domain of either the receptor or some associated protein that is different from the G-protein.
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A case of paroxysmal cold hemoglobinuria (PCH) occurring in a patient with Klebsiella pneumonia is described. The first sign of PCH in the patient was erythrophagocytosis. The Donath-Landsteiner (D-L) antibody exhibited anti-P specificity both serologically and biochemically. The appearance of D-L antibody during fulminating Klebsiella pneumonia strongly suggests their causal relationship.
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