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K McCullough

Publications and source records attributed to K McCullough.

27 records · Page 2Linked to original sources
PubMed1995

Injuries to the ulnar carpometacarpal region: are they being underdiagnosed?

Thirteen cases with radiographic evidence of injury to the ulnar side of the hand are reviewed. These injuries included intraarticular fractures and/or dislocations of the fourth and fifth carpometacarpal joints and associated bones collectively discussed here as the ulnar carpometacarpal region. In nine the extent of the injury was not appreciated on first presentation. Injuries to this region are often difficult to assess clinically and radiographically even in experienced hands. In our experience computerized tomography (CT) scans in the longitudinal and longitudinal oblique axis best display the ulnar carpometacarpal joint surfaces and their relationship to each other. We recommend CT of the ulnar carpometacarpal region where clinical evidence of an injury to this region is not in keeping with the plain radiographic findings, and when planning surgery on a complex injury in this region.

Adolescent↗
PubMed1994

The antibody MY4 recognizes CD14 on porcine monocytes and macrophages.

Several monoclonal antibodies directed against the human CD14 antigen have been established. We now report that the antibody My4, but not LeuM3, reacts with porcine monocytes. Among porcine peripheral blood mononuclear cells (PBMC), 14.6% of the cells stain with the CD14 antibody My4, which is similar to the percentage obtained with the antiporcine monocyte antibody 74-22-15. Two-colour immunofluorescence reveals that My4 and 74-22-15 antigens are coexpressed on the same cells, and cell sorter-purified My4+ cells exhibit the morphology of monocytes. Whole blood analysis (which also shows staining of granulocytes) reveals that the average percentage of My4+ monocytes amongst all leucocytes is 5.8% with 580 cells/microliters. Furthermore, porcine peritoneal macrophages (PM) and alveolar macrophages (AM), both stain for My4, with a four-fold lower level on AM. Treatment of cells with phosphatidylinositol-specific phospholipase C decreases My4 staining, but does not affect staining with antibody 74-22-15. Immunoprecipitation with the My4 antibody from surface labelled pig mononuclear cells demonstrates a 54 kDa band similar to human CD14, and Western blotting with pig serum demonstrates two bands similar to the alpha and beta forms of human soluble CD14. Finally, the My4 antibody is capable of blocking lipopolysaccharide- (LPS)-induced interleukin-6 production in isolated PBMC. These data show that the My4 antibody recognizes genuine CD14 on porcine monocytes and macrophages.

Animals↗
PubMed1989

Recombinant bovine interferon-gamma enhances expression of class I and class II bovine lymphocyte antigens.

Recombinant bovine interferon-gamma augments expression of class I and class II histocompatibility antigens on the surface membrane of bovine lymphocytes. Immunofluorescence techniques using a series of monoclonal anti-HLA antibodies demonstrate that this enhancement is detectable as early as 24 h after incubation with rBoIFN, while maximum surface expression is obtained within 3-5 days. A concentration as low as 10 units of rBoIFN is effective. Such results may be useful for characterizing the BoLA gene products.

Animals↗
PubMed1989

Effects of acute and chronic acyclovir on canine renal function.

Acyclovir-associated renal dysfunction is difficult to study in humans because it occurs in patient populations who are generally receiving multiple medicines concurrently and dysfunctions occur infrequently. We studied the effects of two regimens of acyclovir treatment, a short-term high dose (210 mg/kg/day for 43 hr) via constant infusion and a chronic lower dose by intermittent infusion (15 mg/kg, 3 times/day for 28 days), on selected renal functions in dogs. Urine concentrating capacity as compared to base-line and controls declined during both the short-term (40% decline) and chronic treatments (36% decline). The persistence of the concentrating deficit in the presence of stimulation with vasopressin suggested that the defect resided in the renal response to vasopressin. Glomerular filtration rates significantly decreased during the acute high-dose treatment (from 104 +/- 15 to 87 +/- 11 ml/min) but not during the chronic low-dose treatment. Both acyclovir treatments were associated with a small but significant decline (approximately 10% for both studies) in the plasma potassium concentrations although an increase in urine potassium clearance could not be demonstrated. We conclude that acyclovir can cause renal dysfunction in healthy animals at plasma concentrations higher but comparable to those achieved clinically. Although both treatment regimens decreased renal function, the shorter high-dose regimen which maintained acyclovir plasma concentrations was more detrimental than the longer exposure to a lower dose of the drug given intermittently. The data suggest that renal dysfunction is not related to a rare sensitivity, as all acyclovir-treated animals showed subtle decrements in renal function.

Acyclovir↗
PubMed1988

Antiviral and antigenic properties of recombinant porcine interferon gamma.

Recombinant porcine interferon gamma (rPoIFN gamma) induced a dose-dependent inhibition of the cytopathic effect produced by vesicular stomatitis virus (VSV) challenge of both homologous and heterologous (bovine) cell lines. In addition, an antiviral effect of rPoIFN gamma was demonstrable against the coronavirus transmissible gastroenteritis virus (TGEV) infection of porcine epithelial cells and of pulmonary macrophages. A rabbit anti-PoIFN gamma antiserum was prepared and shown to specifically neutralize the antiviral effects of natural and recombinant porcine IFN gamma preparations. This antiserum could also neutralize recombinant bovine IFN gamma but not recombinant human IFN gamma. These results suggest antigenic homology of porcine and bovine IFN gamma but antigenic differences between these molecules and human IFN gamma.

Animals↗
PubMed1978

Non-invasive assessment of left internal mammary-coronary bypass patency using the external Doppler probe.

Non-directional blood velocity of left internal mammary bypass grafts was non-invasively studied with the Doppler ultrasonic probe. Thirteen of 14 subjects had angiographic evidence of bypass graft patency and their Doppler signals demonstrated high amplitude phasic blood velocities. A single patient with proximal left internal mammary arterial graft occlusion manifested marked attenuation of Doppler blood velocity signals. It is concluded that this technic offers a potential for ambulatory and in-office screening of internal mammary artery bypass graft function.

Blood Flow Velocity↗
PubMedDate not supplied

Recombinant bovine interferon-alpha I 1 inhibits the migration of lymphocytes from lymph nodes but not into lymph nodes.

Recombinant bovine interferon-alpha I 1 (IFN-alpha) was tested for effects on lymphocyte migration into and out of the lymph nodes of sheep. Chronic lymph drainage methods were used to monitor the efferent lymph of single lymph nodes of unanaesthetized animals. When IFN-alpha was injected into the drainage area of the lymph node, infused directly via an afferent lymphatic, or injected intravenously, a marked decrease in cell output was observed. Nanogram quantities of IFN-alpha produced an effect that lasted several hours. To test whether this effect was specific for lymphocytes, we first stimulated a lymph node with Staphylococcus aureus so that large numbers of neutrophils and lymphocytes were present in efferent lymph. When IFN-alpha was then injected, the lymphocytes disappeared from efferent lymph but the neutrophils did not. Normal efferent lymph cells were labeled with radioisotopes and injected intravenously during the IFN-alpha induced effect. The entry of lymphocytes from the blood was significantly enhanced at a time coinciding with the suppressed cell exit in the efferent lymph. Recovery data indicated that the lymphocytes were retained temporarily in the lymph node but were not destroyed. Furthermore, the bovine IFN-alpha does not appear to be conspicuously antigenic in sheep. It is not yet certain whether IFN-alpha enhances the interactions of cells within the lymph node thereby potentiating immunologic reactions.

Animals↗