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Biomedical subjects

K Matsuda

Publications and source records attributed to K Matsuda.

At least 793 records · Page 44Linked to original sources
PubMed1980

Characterization of the multiple forms and main component of dextransucrase from Leuconostoc mesenteroides NRRL B-512F.

Multiple forms of dextransucrase (sucrose:1.6-alpha-D-glucan 6-alpha-D-glucosyltransferae EC 2.4.1.5) from Leuconostoc mesenteroides NRRL B-512F strain were shown by gel filtraton and electrophoretic analyses. Two components of enzyme, having different affinities for dextran gel, were separated by a column of Sephadex G-100. The major component voided from the Sephadex column was treated with dextranase and purified to an electrophoretically homogeneous state. The ]urified enzyme had a molecular weight of 64 000-65 000, pI value of 4.1, and 17% of carbohydrate in a molecule. EDTA showed a characteristic inhibition on the enzyme while stimulative effects were observed by the addition of exogenous dextran to the incubation mixture. The enzyme activity was stimulated by various dextrans and its Km value was decreased with increasing concentration of dextran. The purified enzyme showed no affinity for a Sephadex G-100 gel, and readily aggregated after the preservation at 4 degrees C in a concentrated solution.

Dextranase↗
PubMed1980

Non-selective distribution of hypoglossal nerve fibers after section and resuture: a horseradish peroxidase study in the cat.

The retrograde tracing method of horseradish peroxidase (HRP) was applied to examine the process of regeneration of severed hypoglossal nerve in the cat. After section and resuture of the hypoglossal nerve, the cats were allowed to survive for 4-6 months. In these cats, distribution of neurons labeled with HRP injected into the genioglossus muscle was examined and compared with that in the normal cat. In the operated cats, labeled genioglossus motoneurons were scattered within all subdivisions of the hypoglossal nucleus, indicating non-selective distribution of regenerating hypoglossal nerve fibers to the lingual muscles.

Animals↗
PubMed1980

Dissecting aneurysm during pregnancy and the puerperium.

According to Schnitker, Mandel, Hirst and their associates, approximately half of the dissecting aneurysms in women under 40 years of age are associated with pregnancy. This significant relationship between dissecting aneurysm and pregnancy has been discussed by considering hemodynamic stress and also the hormonal changes of pregnancy. In this report, we describe five patients with dissecting aneurysm during pregnancy or the puerperium, review the literature and discuss the influence of pregnancy on the pathogenesis of this disease.

Adult↗
PubMed1980

Experimental gingivitis in ODU plaque-susceptible rats. V. On the vascular permeability factor of dental plaque.

Plaque-induced inflammation has been documented from various aspects, but is still not well understood. In an effort to clarify the physicochemical properties of dental plaque and to isolate the active factor which give rise to inflammation, we studied a factor in plaque which may be a direct and local cause of gingival inflammation in ODU rats. Rat dental plaque was dissolved in physiologic saline. After being homogenized, the solution was centrifuged and the supernatant (RP) was used as a sample. Protein component (pRP) of PR was isolated by ammonium sulfate fractionated procedure and then deproteinized RP (dpRP) was obtained by trichloroacetic acid treatment. The vascular permeability factor existed in RP and it was affected by heating and pH. Changes in vascular permeability were directly proportionate to the logarithm of the concentration of protein fraction in dental plaque, and were of a delayed type. It was surmised that pRP contained in RP was the main vascular permeability factor, and it was purified by gel filtration on Sephadex G-100 and G-200.

Animals↗
PubMed1980

Studies on cellulases of a phytopathogenic fungus, Pyricularia oryzae Cavara. IV. Kinetic studies on beta-glucosidases.

Kinetic studies of the two beta-glucosidase isozymes, GB-1 and GB-2, which were from the culture filtrate of a phytopathogenic fungus Pyricularia oryzae, revaled that the latter isozyme was an allosteric protein with two substrate binding sites. The homotropic effects of o- and m-nitrophenyl-beta-D-glucosides on GB-2 showed positive cooperativity, whereas that of cell cellooligosaccharide showed negative cooperatively. The affinity of GB-2 for cellooligosaccharide tended to increase with decreasing chain length, in contrast to that of GB-1. Glucono-delta-lactone and glucose acted as competitive inhibitors of GB-1 and GB-2. As regards the control of the level of glucose formed by the cellulose system, it appears that the rate of formation of glucose by beta-glucosidase is reduced by the presence of the substrate, cellooligosaccharide, as well as by the product, glucose.

Allosteric Regulation↗
PubMed1980

Clinical, hematological and pathological responses in severely heat-stressed steers with special reference to the threshold value for survival.

To find out a critical point of physiological responses of steers which would finally lead to death due to heat stress, four Holstein steers weighing about 300 kg were housed in an artificial climatic room and exposed to each of the following room temperature and relative humidity: 35 degrees C and 70 per cent, 36 degrees C and 80 per cent, 38 degrees C and 70 per cent, and 42 degrees C and 60 per cent. Sequential clinical and hematological examinations were carried out during thermal loads on the steers, and pathological examinations after their death or slaughter. All the steers manifested panting, rise of rectal temperature, reduction or disappearance of peristalsis, hypersalivation, dysfunction of the skin reflex, and remarkable decrease in white blood cell count and pressure of CO2 (Pco2) value of jugular blood. At the end of heat stress, an animal would be survived if it meet the following conditions: (1) rectal temperature was below 42.7 degrees C, (2) the respiration rate did not decrease to one half (about 100/minute) of the maximum rate during the exposure period, (3) peristalsis and skin reflex disappeared almost completely, (4) the white blood cell count did not decrease to less than 50 per cent of the normal level, and (5) the Pco2 value of venous blood was maintained at a level of more than 15 mmHg. Cloudy discoloration with a boiled-meat appearance was noticed in the skeletal muscle of the hind part of the body in two dead cases. No significant changes were detected in any other steer slaughtered several days after release from the thermal stress.

Animals↗
PubMed1980

[A case of intracranial chordoma with a special reference to the findings in computed tomography (author's transl)].

A case of chordoma was reported with a special reference to the computed tomography. A 22-year-old female, who had been in good health, was admitted to Kagoshima University Hospital on December 8, 1978 with a chief complaint of diplopia. Physical examination was nothing particular and neurologic examination revealed the left abducent nerve palsy. The labolatory findings including blood count, serum electrolytes, hormonal study, carcinoembrynonic antigen (CEA) and alpha-fetoprotein were within normal limits. Plain skull filsm showed a retro-sellar calcification in combination with a bony erosion of the dorsum sellae and the clivus. The left vertebral arteriography and the left carotid arteriography showed a space taking lesion extended from the retro-clival to the left parasellar region. The axial CT scanning showed a round and irregular high density area consistent with calcification, at the retro-clival area, middle cranial fossa, and parasellar region in the left side, and no enhancement effect was acquired. The coronal CT appearance showed a round but discontinuous high density area like a coral reef, based on the clivus. These CT findings indicated the extension of the space taking legion, clearly. Intra-capsular removal of the tumor was performed and histologic examination confirmed the chordoma. It will be emphasized that CT scan, particularly coronal section was one of the most useful diagnostic procedure of amss of the skull base.

Adult↗
PubMed1979

Topographical arrangement of hypoglossal motoneurons: an HRP study in the cat.

Myotopical localization of hypoglossal motoneurons and representation of the main branches of the hypoglossal nerve within the hypoglossal nucleus were examined in the cat by the HRP method. The hypoglossal nucleus is divided cytoarchitectonically into the ventromedial and dorsolateral divisions; the medial and lateral branches of the hypoglossal nerve are represented respectively in the ventromedial and dorsolateral divisions. The genioglossus motoneurons are located in the ventrolateral part of the ventromedial division, and the geniohyoid motoneurons are in the most ventral part of the ventromedial division. The hypoglossus and styloglossus motoneurons are located in the lateral and dorsolateral parts of the dorsolateral division.

Animals↗