[A case of gastrointestinal GVHD (graft-versus-host disease) and cytomegalovirus ileitis with bone marrow transplantation].
Explore the source record for details and available documents.
Adult↗
Biomedical subjects
K Maeda
Publications and source records attributed to K Maeda.
[Two cases of thymic cysts and review of the 220 Japanese cases].
Thymic cyst is relatively rare disease among mediastinal tumors. We reported two cases of surgical resected thymic cysts. Case 1: A 72-year-old female was found to have an abnormal shadow at right cardio-phrenic angle on chest X-ray film. Operative and pathological diagnosis was thymic cyst. Immunohistochemical study showed the epithelial cells lining the cyst to contain keratin and CEA but not CA19-9. Case 2: A 67-year-old male was pointed out to have an abnormal shadow at left mediastinum on a chest X-ray film during his stay in our hospital for acute myocardial infarction. Preoperative diagnosis was made to be a thymic cyst and operation was done. Pathological diagnosis was the same. Behçet's disease was accompanied in this patient. It is very rare to be complicated with thymic cyst. We reviewed 220 operated cases of thymic cyst in the Japanese literature.
Aged↗
[Immunohistochemical study on the distribution of CGRP-containing nerve fibers in the PCB poisoned rats with experimental periodontitis].
We have studied immunocytochemically the distribution of CGRP-containing nerve fibers in the PCB poisoned rats introducing experimental periodontitis. The results obtained were as follows. In the PCB poisoned rats, some specimens obtained from periodontitis-affected sites showed a slight increase in both the intensity of nerve staining and the nerve sprouting. However, no discernible difference in the occurrence and the distribution of nerves has been demonstrated between the PCB poisoned rats and clinically healthy rats. Further studies are needed to confirm the effects of PCBs on the initiation and progression of periodontitis.
Animals↗
[A case of transient fetal hydrops which would be caused by parvovirus B19 infection].
Explore the source record for details and available documents.
Erythema Infectiosum↗
Inhibitory effect of FUT-175 on complement activation and its application for glomerulonephritis with hypocomplementemia.
FUT-175 (6-amidino-2-naphthyl p-guanidinobenzoate dimethane-sulphonate), a potent serine protease inhibitor, has been reported to inhibit complement activity in vitro, and especially the classical complement pathway effectively. In the present study, we examined the inhibitory effect of FUT-175 on the classical complement pathway components by hemolytic assay using purified human complement components. As a result, 50% inhibition of the C1 protease activity for classical C3 convertase formation and for C2 was obtained with 3.0 x 10(-8) M and 7.0 x 10(-8) M of FUT-175, respectively. FUT-175 did not inhibit the C2 protease activity at all. We then administered FUT-175 to 5 glomerulonephritic patients with hypocomplementemia and proteinuria in order to assess the clinical effectiveness of this drug. When FUT-175 was administered intravenously and continuously at a rate of 0.1 to 0.2 mg/kg/hr for 2 weeks, the urinary protein excretion decreased significantly from 2.9 +/- 0.8 to 1.4 +/- 0.5 g/day (P < 0.025). In these patients, some of the serum complement markers (serum C3, C4 level and the hemolytic activity via the classical complement pathway (CH50)) were increased after FUT-175 administration. The above findings suggests that FUT-175 can exert beneficial effects on glomerulonephritis with hypocomplementemia by inhibiting complement activation.
Adult↗
[Successful chronic daily administration of oral etoposide for a case of adult T cell leukemia].
A 54-year-old woman with leucocytosis and skin lesion was hospitalized and diagnosed as chronic type adult T cell leukemia (ATL) in August 1989. Since her ATL cell count and LDH level increased after hospitalization, oral administration of etoposide was started at a dose of 100 mg/day for seven days. The oral administration of etoposide induced another chronic state of ATL. After 10 months without medication, she was readmitted because of an acute ATL crisis. After daily administration of etoposide at a dose of 50 mg/day, the white blood cell count and serum LDH level decreased to the normal range, and abnormal lymphocytes of peripheral blood disappeared. The low-dose daily administration of etoposide at a dose of 25 approximately 50 mg/day could be maintained over six months. No severe side effects except for alopecia and mild myelosuppression were noted during the treatment. Chronic daily administration of oral etoposide is one candidate for the treatment of ATL in an outpatient clinic.
Administration, Oral↗
[Skin reaction(s) to culture supernatant(s) from Dermatophagoides farinae (Df)-stimulated lymphocytes, and their correlation with Df-induced interleukin 2 responsiveness by lymphocytes from patients with bronchial asthma].
To investigate the inflammatory function of Dermatophagoides farinae (Df)-activated lymphocytes, supernatants from cultures of the lymphocytes were intracutaneously injected into donors' forearms. Injection of the supernatants of Df-stimulated lymphocytes from mite-sensitized atopic individuals with such diseases as bronchial asthma induced a profound inflammatory reaction in the autologous skin, characterized by erythema extending more than 15 mm in mean diameter and edema. The inflammation was at its peak after approximately 20 min, which was followed by gradually re-growing erythema lasting for as long as 24 hours after injection. Supernatants of unstimulated lymphocytes were also capable of inducing the same reaction, indicating the presence of in vivo activated lymphocytes, although the extent of the response was always smaller. The induced response in normal individuals was erythema of less than 15 mm in mean diameter. The supernatants obtained from cultures at 4 degrees C failed to induce such inflammation. The culture supernatants of ovalbumin-restimulated lymphocytes were also incapable of augmenting the response. The combined data show that the production of inflammatory response-inducing factor(s) from Df-stimulated lymphocytes was antigen specific. Significant skin reactions was correlated with the IL2 responsiveness of Df-stimulated lymphocytes. The skin reaction induced by factor(s) derived from Df-stimulated lymphocytes, which might be similar to the bronchial hyperreactivity of patients with bronchial asthma. The in vitro assay for Df-induced IL2 responsiveness by lymphocytes might reflect the in vitro immediate, late and/or delayed type hypersensitivity.
Animals↗
[Dialysis therapy--1992].
Explore the source record for details and available documents.
Diabetic Nephropathies↗
Hypotonic cisplatin treatment for carcinomatous pleuritis found at thoracotomy in patients with lung cancer. In vitro experiments and preliminary clinical results.
We have developed an intraoperative intrapleural treatment with the use of distilled water combined with cisplatin for carcinomatous pleuritis found at thoracotomy in patients with non-small-cell lung cancer. In the in vitro experiment, three different cell lines were used as a model of malignant pleural effusion. Cell growth was examined after a 3-day culture, which was preceded by exposure of the cells to cisplatin in either phosphate-buffered saline solution or distilled water for 1/2 to 5 minutes. The growth inhibition of tumor cells after hypotonic cisplatin treatment was significantly greater than after treatment with saline solution and cisplatin. Tumor that was obtained by resection of non-small-cell lung cancer was used as a model to demonstrate decreased viability of the tumor after exposure to hypotonic cisplatin. The viability of the tumor in a 6-day culture, preceded by exposure to hypotonic cisplatin (50 micrograms/ml) for 10 minutes, was markedly decreased. Intraoperative intrapleural hypotonic cisplatin was instilled in seven patients with pleural carcinomatosis without side effects and with control of pleural dissemination and pleural effusion for 6 to 29 months.
Aged↗
Is T factor of the TNM staging system a predominant prognostic factor in pathologic stage I non-small-cell lung cancer ? A multivariate prognostic factor analysis of 151 patients.
We attempted to clarify whether the T factor of the TNM staging system should be viewed as a predominant prognostic factor in patients with pathologic stage I non-small-cell lung cancer when analyzed together with various histopathologic factors and deoxyribonucleic acid ploidy pattern of tumors. We studied 151 patients who were in this stage. Histopathologic factors used in the analysis were as follows: histologic cell type (squamous or nonsquamous cell carcinoma), grade of differentiation, and tumor invasion of visceral pleura and vessels. Deoxyribonucleic acid ploidy pattern of tumors was analyzed by flow cytometry, and the tumors were classified as diploid or aneuploid tumors. Significant prognostic factors (p < 0.05) that were demonstrated by univariate analysis of survival curves were as follows: (1) T1 versus T2; (2) well versus moderately or poorly differentiated tumor; (3) the absence versus presence of tumor exposed on pleura, (4) artery invasion, (5) lymphatic vessel invasion; and (6) diploid versus aneuploid tumor. Multivariate prognostic factor analysis showed the grade of differentiation and deoxyribonucleic acid ploidy pattern to be predominant prognostic factors. The T2 tumor group had significantly more cases with tumor invasion of lymphatic vessels that did the T1 tumor group and included 18 cases with tumor exposed on pleura. When these two factors were excluded from multivariate analysis, the T factor was marginally significant (p = 0.08). These observations suggest that the T factor is not necessarily a predominant prognostic factor in pathologic stage I non-small-cell lung cancer.
Adult↗
[Transcatheter arterial chemoembolization to hepatic metastases from colorectal cancer].
Response of transcatheter arterial chemoembolization (TAE) and transcatheter arterial infusion chemotherapy to hepatic metastases was reported in 25 cases of colorectal cancer. The severity of liver metastases was H1 in 12 cases, H2 in 9 cases, and H3 in 4 cases. Liver metastases were found during surgery in 12 of these patients, and 13 showed metastases or recurrence in the liver after resections of primary lesions. Catheters were inserted selectively to the proper hepatic artery by Seldinger's method, followed by injection of embolizing agents (gelfoam particles of Lipiodol) with adriamycin or 5-FU + leucovorin in most cases. Response was assessed by blood CEA levels, diagnostic imaging, and period of survival. In 5 cases in whom liver resections were performed following TAE, response was assessed by histopathological findings of the resected specimens. Two patients showed partial response (PR), 12 no change (NC) and 11 progressive disease (PD) by diagnostic imaging. Blood CEA levels fell to less than 50% of pre-treatment levels in 26% of cases. Histological changes by TAE were confirmed in 4 of 5 cases after liver resections, but viable cancer cells were observed in all cases. A case of mucinous cancer showed no change histologically. As the other case of mucinous cancer showed PD by diagnostic imaging, TAE was not suggested to be suitable to treat cases of mucinous cancer. More improvements in the dosage, drugs and times of treatment were suggested to yield a better response rate in TAE therapy for liver metastases from colorectal cancer.
Adult↗
[Effect of rapid mannitol infusion on middle cerebral artery blood flow velocity and pulsatility index--a transcranial Doppler ultrasonography study in monkeys].
Hemodynamic changes immediately after the administration of mannitol were investigated in monkeys (Macaca Fuscata) using transcranial Doppler ultrasound (TC2-64) and were compared with those after CO2 loading. The CO2 loading group was hyperventilated to an end-tidal PCO2 of 36.5 +/- 2.21 and hypoventilated to an end-tidal PCO2 of 46.3 +/- 2.69. Mean flow velocity in the middle cerebral artery (MCA-FV), Pulsatility index (PI) and blood flow in the internal carotid artery (IC-BF) studies were performed twice before and after hypercapnea. In the Mannitol group, Mannitol (2 g/kg) was infused at a rate 20 to 30 ml/min. MCA-FV, PI, IC-BF and intracranial pressure (ICP) studies were performed twice before administration and 10 minutes after administration of Mannitol. In the Mannitol group (n = 8), MCA-FV and IC-BF and ICP increased significantly after administration of Mannitol (p < 0.005, p < 0.005, p < 0.025), but there was no significant difference in the PI. In the CO2 loading group (n = 15), MCA-FV and IC-BF increased significantly (both p < 0.005) and PI decreased significantly (p < 0.005). These results indicated that, in the presence of normal autoregulation without intracranial hypertension, there was no change in the resistance of the peripheral cerebral vessels, while a decrease in viscosity after administration of Mannitol caused an increase in cerebral blood flow. Hence, according to Poiseuille's law, peripheral vasoconstriction occurred in the early period after administration of Mannitol. This change was considered to be due to autoregulation of the cerebral vessels.
Animals↗
Inhibition of mitochondrial respiration by furancarboxylic acid accumulated in uremic serum in its albumin-bound and non-dialyzable form.
3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF) accumulates markedly in uremic serum in its albumin-bound form. To determine if CMPF can be removed by newly developed dialyzers with high-flux membranes which are permeable to low-molecular-weight proteins, such as beta 2-microglobulin (beta 2-MG), serum levels of CMPF were determined before and after hemodialysis using these high-flux membrane dialyzers. In addition, to determine the pathogenic role of CMPF in uremic patients, its cellular toxicity due to its effect on mitochondrial respiration was studied. The reduction rates of CMPF by hemodialysis using the dialyzers ranged from -17% to -24%, demonstrating the nondialyzability of CMPF due to its strong albumin-binding, while those of beta 2-MG ranged from 11% to 43%. CMPF inhibited ADP-stimulated oxidation of NADH-linked substrates in isolated mitochondria dose-dependently regardless of the presence of serum albumin. This inhibition was observed even at a concentration of 0.2 mM, which is comparable to the serum levels of CMPF in the hemodialysis patients. In conclusion CMPF which cannot be removed even by high-flux membrane dialyzers, is a strong inhibitor of mitochondrial respiration, and novel purification methods to remove CMPF from the blood of uremic patients should be developed.
Animals↗
[Analysis of cell proliferation in endoscopically biopsied specimen of the stomal mucosa of the remnant stomach--with special reference to cell cycle and ODC activity].
An attempt was made to investigate the status of cell proliferation in the remnant stomach, using endoscopically biopsied specimens, with cell cycle analysis by flow cytometry (FCM) and ornithine decarboxylase (ODC) activity determination. Fifty-three partially gastrectomized patients (male 33, female 20) were examined. Their mean age was 60.5, and 22 underwent Billroth I (BI), and 30 Billroth II (B II) operation. The mean time elapsing from the initial operation was 4.0 years. Almost all specimens showed DNA diploidy by FCM. The S phase cell percentage was higher in the stomal area, especially in the B II group. Significantly higher ODC activity was recognized in the stomal mucosa especially in the B II group. A significant positive relationship was noted between postoperative duration and ODC activity in the B II group. These results suggest activated cell proliferation in the stomal area of the remnant stomach. Especially in the B II group, cell proliferation increased with time after initial operation. This activated cell proliferation may play some role in the carcinogenesis of the remnant stomach.
Biopsy↗
Recent advances in diagnosis of leukemia.
With the development of new technologies in the diagnosis of leukemia, we have modified the method of approaching individual cases. In addition to morphological examination, cytochemistry, immunophenotyping, electron microscopy and cytogenetics are now routinely used for daily practice. Furthermore, various molecular diagnostic methods are being tested, and some are already being used for diagnostic processes. The new technologies have certainly changed the diagnostic approach. When the diagnosis is supported by such multiple diagnostic methods, it is more reliable, reproducible and standardized. The communication among clinical practitioners through accurate diagnosis is valuable from both the therapeutic and prognostic point of view. In this article, the diagnostic method of leukemia is reviewed with an emphasis on practical aspects. Individual details are beyond the scope of this article. Interested readers are referred to the bibliography for more in depth treatment of the various techniques.
Flow Cytometry↗
Effect of serotonergic agents on regional concentrations of somatostatin- and neuropeptide Y-like immunoreactivities in rat brain.
A possible role for neuropeptides in affective disorders is suggested by many investigators. Somatostatin-like immunoreactivity (SS-LI) and neuropeptide Y-like immunoreactivity (NPY-LI) concentrations are demonstrated to be reduced in cerebrospinal fluid from depressed patients. We have shown that long-term treatment with serotonin uptake inhibitors, clomipramine and zimelidine, reduce brain SS-LI concentrations in the rat. We have studied the effect of serotonergic agents on regional brain SS-LI and NPY-LI concentrations in rats. Long-term treatment with 5-hydroxytryptamine (5-HTP), a serotonin precursor, caused reductions in SS- and NPY-LI levels in the hypothalamus. SS- and NPY-LI concentrations in the brain were markedly elevated by treatment with p-chlorophenylalanine, a serotonin synthesis inhibitor. Intracerebroventricular administration of 5,7-dihydroxytryptamine, a serotonin neurotoxin, resulted in elevations of both peptides in the brain. These results suggest a inhibitory role for the serotonergic system in the brain in the regulation of SS and NPY.
5,7-Dihydroxytryptamine↗
Plasma prolactin as a predictor of relapse in drug-free schizophrenic outpatients.
A low plasma prolactin concentration has been reported to be associated with an increased risk of subsequent relapse in patients with schizophrenia. Prolactin concentration was measured in samples from stable schizophrenic men who were outpatients just prior to neuroleptic withdrawal. No relationship between prolactin concentration and time to subsequent relapse was found. Prolactin concentration may predict time to relapse only in populations characterized by specific demographic features or medication history.
Administration, Oral↗
Double antenna structure of chicken prolactin receptor deduced from the cDNA sequence.
Chicken prolactin receptor (cPRLR) deciphered from the cDNA sequence showed a unique double antenna structure in its extracellular domain. The predicted cPRLR preprotein was composed of 831 amino acids and contained a signal peptide and a transmembrane region. The extracellular domain comprised 438 residues, and was divided into two tandemly repeated, highly homologous units, each of which corresponded to the extracellular domains of mammalian prolactin receptors. Both extracellular units of cPRLR possessed two structural features characteristic of the ligand binding units of cytokine/prolactin receptor family, namely two pairs of cysteine residues and a WSXWS motif. These findings strongly suggest that cPRLR contains two repeated ligand binding units, that is a double antenna structure. The cPRLR gene is expressed in a wide range of tissues of laying hen.
Amino Acid Sequence↗