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Biomedical subjects

K M Kim

Publications and source records attributed to K M Kim.

At least 127 records · Page 7Linked to original sources
PubMed1998

Psoriasiform eruption triggered by recombinant granulocyte-macrophage colony stimulating factor (rGM-CSF) and exacerbated by granulocyte colony stimulating factor (rG-CSF) in a patient with breast cancer.

Colony-stimulating factors (CSFs) are commonly used for the treatment of neutropenia following chemotherapy and for the mobilization of peripheral blood stem cells (PBSC). We recently experienced a rare case of a new onset of psoriasiform eruption by GM-CSF (granulocyte-macrophage colony-stimulating factor) which was exacerbated by G-CSF (granulocyte colony-stimulating factor) in a patient with breast cancer. A 36-year-old woman had received neoadjuvant chemotherapy (cyclophosphamide, epirubicin and 5-fluorouracil), modified radical mastectomy and adjuvant chemotherapy with paclitaxel and mitoxantrone followed by GM-CSF administration for the treatment of locally advanced breast cancer. She had developed a psoriatic skin lesion on face and both upper arms during leukocyte recovery in spite of no previous history of psoriasis. Next, the chemotherapy course was complicated by a flare of mild psoriatic skin lesion, although CSF was changed into G-CSF due to GM-CSF-associated psoriasis. Subsequently, she had had high-dose chemotherapy and autologous peripheral blood stem cell transplantation for consolidation therapy. GM-CSF was administered for the mobilization of PBSC and post-transplant period, but psoriatic skin lesion did not appear. During 6 months after PBSCT, psoriasiform eruption did not appear.

Adult↗
PubMed1998

Development of a medical record and radiographic image transmission system using a high-speed communication network.

A medical record and radiographic image transmission system has been developed using a high-speed communication network. The databases are designed to store and transmit the data acquired from the scanner. To maximally utilize the communication bandwidth, the medical records and radiographic images are compressed using the G3 facsimile and JPEG coding standard method, respectively. TCP/IP, OOP and Windows-based system software enable a modular design, future expandability, open system interconnectivity, and diverse image manipulation functions.

Computer Communication Networks↗
PubMed1998

Implementation of a low-cost PACS/CR for clinical use in Yonsei Cardiovascular Center.

The PACS/CR for clinical use in Yonsei Cardiovascular Center has been designed and implemented. Our system is open architecture to comply with emerging standards such as DICOM. database SQL, TCP/IP and to reduce operational and maintenance costs, PC-based low cost workstations running Microsoft Windows, database as Microsoft SQL based on Client/Server, Long-term storage using CD-ROM Jukebox are developed. Also, auto routing and image pre-fetching are implemented.

Ambulatory Care Facilities↗
PubMed1998

Recent advances with chemotherapy for NSCLC: the ECOG experience. Eastern Cooperative Oncology Group.

Management of disseminated non-small-cell lung cancer has changed over the past 10 years. Newer agents, such as vinorelbine (Navelbine) and paclitaxel (Taxol), have been shown to modestly improve survival in patients with advanced disease when administered in conjunction with cisplatin (Platinol). Compared with older regimens consisting of cisplatin and a Vinca alkaloid or a podophyllotoxin, the newer regimens yield a 10- to 15-week improvement in median survival and an additional 10% to 15% in 1-year survival. Based on these results derived from randomized trials, it appears that metastatic non-small-cell lung cancer patients with good performance status should be treated with regimens containing either vinorelbine or paclitaxel in conjunction with cisplatin.

Antineoplastic Agents, Phytogenic↗
PubMed1998

Detection of soluble Fas mRNA using in situ reverse transcription-polymerase chain reaction.

Fas protein (Fas) is known to induce cell death by apoptosis in susceptible cells. Alternative splicing of the Fas gene produces soluble Fas protein (sFas), which is considered to block the function of Fas. The serum level of sFas is elevated in patients with various malignancies in a manner reflective of disease stage and tumor burden, but the precise cellular origin of sFas in vivo has not yet been clarified. To identify the cells that synthesize sFas mRNA on histologic specimens, we applied in situ reverse transcription-polymerase chain reaction (in situ RT-PCR) in 11 cases of gastric adenocarcinoma/metastatic lymph node. Furthermore, we studied the distribution of Fas using immunohistochemistry and Fas mRNA using in situ RT-PCR. In all primary tumors and 10 of 11 metastatic tumors, tumor cells expressed both Fas- and sFas mRNA. Lymphocytes infiltrated in the tumor tissues and the lymph nodes also revealed both mRNA signals. A clear correlation between the tissue distribution for Fas and its mRNA was also observed. These observations demonstrated that solid tumors in vivo can synthesize sFas mRNA and suggest that tumor cells are responsible in part for elevated sFas in human malignancies. However, the additional expression of sFas mRNA in tissue lymphocytes indicates the complex regulatory mechanisms of Fas-mediated apoptosis pathway in tumor pathogenesis and host defense. We also demonstrated that in situ RT-PCR can be a suitable method for in situ detection of alternatively spliced mRNA.

Alternative Splicing↗
PubMed1998

Cloning, gene structure and genomic localization of an orphan transporter from mouse kidney with six alternatively-spliced isoforms.

Two genes were identified and characterized that express cDNAs related to previously identified neurotransmitter and/or osmolyte transporters, but which are expressed specifically in the kidney. RNA transcribed from one of these two genes (XT2) was found to undergo an extensive degree of alternative splicing to generate six distinct isoforms. The intron-exon structure of the XT2 gene and the sites of alternative splicing were identified. Expression of the second gene (XT3) was found to be conserved in human kidney, and partial sequence was obtained from a human cDNA library. The expressions of both XT2 and XT3 RNAs were determined in mouse and human tissues, respectively, and the locations of the two genes within the mouse genome were identified. Screening experiments to identify the substrate(s) of these proteins failed to identify specific uptake with any of the tested compounds; however, immunofluorescent microscopy demonstrated that epitope-tagged variants of the protein products of the XT2 and XT3 cDNAs were present on the plasma membrane of transfected cells.

Alternative Splicing↗
PubMed1997

Dopamine and GABA receptors in cultured substantia nigra neurons: correlation of electrophysiology and immunocytochemistry.

Primary neuron cultures were made separately from the substantia nigra pars compacta and the substantia nigra pars reticulata of neonatal rats. Using the whole-cell patch-clamp, we tested for the presence of dopamine and GABA receptor subtypes by applying dopamine receptor agonists [the D2 receptor agonist quinpirole and the D1 receptor agonist R(+)-SKF-38393] and GABA receptor agonists (GABA and baclofen). The D2 agonists and the GABA(B) agonist increased an inward rectifier K+ conductance, while the D1 agonist decreased this K+ conductance. Application of GABA increased membrane conductance, probably by increasing Cl- permeability through GABA(A) receptors. Following the physiological tests, the same neuron was examined by double immunocytochemical labeling for antibody to tyrosine hydroxylase and antibody to GABA. Neurons which responded to the D2 agonist were dopaminergic neurons, while neurons which did not respond to D2 agonist were mostly GABAergic or non-dopaminergic/non-GABAergic. Neurons which responded to the D1 agonist were non-dopaminergic/non-GABAergic. GABA(A) receptors were present in all types of neurons, while GABA(B) receptors were located on some dopaminergic neurons and some GABAergic neurons. These results largely agree with the published data on in vivo or brain slice preparations, indicating that these neurons of neonatal rat brains, after being dissociated, produce the same transmitters and the same receptors in culture as those in vivo.

Animals↗
PubMed1997

Hemodynamic changes after complete unilateral ureteral obstruction in the young lamb.

PURPOSE: We studied renal hemodynamic changes after complete acute unilateral ureteral obstruction in the young lamb. MATERIALS AND METHODS: Seven 4-week-old lambs underwent unilateral complete ureteral obstruction and renal blood flow was monitored in both kidneys with ultrasonic flow transducers. Ureteral and arterial blood pressure was measured. RESULTS: In the obstructed kidney renal blood flow was stable for 5 hours and then decreased thereafter to 71% of baseline at 5 days (p < 0.05). In the contralateral kidney the pattern of renal blood flow constituted a mirror image, although the magnitude of the increase was decreased (122% of baseline at 5 days, not statistically significant). Mean arterial blood pressure did not change in 5 days. Mean ureteral pressure increased from a baseline of 9 to 37 mm. Hg (p < 0.001) 2 hours after obstruction but gradually decreased to 24 mm. Hg after 24 hours and remained elevated for 5 days (p < 0.001). CONCLUSIONS: The pattern of renal blood flow and ureteral pressure response to obstruction in the young lamb is different from that previously reported. Renal blood flow does not increase after obstruction and it is relatively preserved at 5 days. This unexpected response may be a result of the compound caliceal sheep kidney, immaturity of the young vascular system or development differences in the mechanism of spontaneous decompression of the collecting system.

Age Factors↗
PubMed1997

Superior laryngeal nerve brain stem evoked response in the cat.

The generator sources of each wave of the laryngeal brain stem evoked response (LBR) have yet to be precisely demonstrated, although this has been studied in several animal species. This study was carried out to record the near-field brain stem activity as well as the far-field brain stem activity in the cat under the same experimental setup, and to search for the generator sources of the waves. Under general anesthesia, the LBR tracings were recorded adjacent to and within the brain stem following direct electrical stimulation of the internal branch of the superior laryngeal nerve. Reproducible positive and negative waves were detected by the far- and near-field techniques. Mean latencies, configurations, and reproducibility of each wave were demonstrated. From these results, we speculate on the generator sources of each wave.

Animals↗
PubMed1997

Increase in swimming endurance capacity of mice by capsaicin-induced adrenal catecholamine secretion.

Increase in endurance swimming capacity caused by capsaicin (CAP), a pungent component of red pepper, -induced increase of fat metabolism in mice was investigated using an adjustable-current water pool. The mice administered CAP via a stomach tube, showed longer swimming time until exhaustion than the control group of mice, in a dose-dependent manner. The maximal effect was observed at a dose of 10 mg/kg while more than 15 mg/kg had no effect. The increase of endurance was observed only when CAP was administered two hours before swimming. After the administration of CAP, the serum glucose concentration rapidly increased and then decreased within 60 min, while the concentration of serum-free fatty acids gradually increased through 3 hours. The residual glycogen concentration of the gastrocnemius muscle after 30 min of swimming was significantly higher in the CAP-administered mice than in control mice, suggesting that use of the serum free fatty acids spared muscle glycogen consumption. The serum adrenaline concentration significantly increased with twin peaks at 30 min and two hours after administration of CAP. An experiment using adrenalectomized mice was done to confirm that the effect of CAP is due to increased energy metabolism through the secretion of adrenaline from the adrenal gland. The swimming endurance capacity of the adrenalectomized mice was not increased by CAP administration, although adrenaline injection induced a 58% increase in the endurance time. These results suggest that the increase of swimming endurance induced by CAP in mice is caused by an increase in fatty acid utilization due to CAP-induced adrenal catecholamine secretion.

Animals↗
PubMed1997

Carcinoma ex pleomorphic adenoma of the palate--a case report.

A case of squamous cell carcinoma ex pleomorphic adenoma in a palate is presented and comments on diagnostic criterias are described. The patient was 36-year-old male presenting with an ovoid elevated palate mass for 6 months. The tumor located in the junctional area of soft and hard palate. The mucosa was diffusely ulcerated and the mass focally tightly adherent to adjacent tissue. The initial cytologic and pathological diagnosis by fine needle aspiration biopsy and open biopsy was benign pleomorphic adenoma. After total removal, histologic examination revealed that tumor was composed partly of benign pleomorphic adenoma and partly of an squamous cell carcinoma component with areas of necrosis and capsular invasion. Immunohistochemical staining in the carcinoma area revealed positive reaction for low and high molecular weight cytokeratin, and epithelial membrane antigen, but negative for desmin, actin, GFAP and S-100 protein. In situ hybridization using biotinylated Epstein-Barr virus probe was done and the neoplastic cells were negative. Our case in an unusual partially encapsulated carcinoma ex pleomorphic adenoma in the palate and is not related in EBV infection.

Adenoma, Pleomorphic↗
PubMed1997

Changes in arterial blood PO2, PCO2, and pH during deep hypothermic circulatory arrest in adults.

Safe limits of time and temperature during sleep hypothermic circulatory arrest (DHCA) still remain controversial. Furthermore, continuous changes of PaO2, PaCO2, and pH have never been measured during DHCA in humans. Continuous intraarterial blood gas (CIABG) monitoring is a new technology allowing us to study chronological changes occurring due to metabolism during DHCA. When the patients' temperature reached 18 approximately 20 degrees C following establishment of cardiopulmonary bypass (CPB), circulatory arrest was initiated. After a 20-minute period of DHCA, reperfusion commenced with 18 degree C blood. We continuously monitored PaO2, PaCO2, and pH immediately before, during and following DHCA. Data was analyzed by Student's t-test. PaO2, PaCO2, and pH of pre- and 5 minutes post DHCA were not significantly different from each other. However, during DHCA, the PaO2 was significantly decreased from 229 +/- 34 to 30 +/- 23 mmHg at 20-minute intervals. But the PaCO2 increased significantly after 20 minutes of circulatory arrest from 34 +/- 5 to 42 +/- 6 mmHg. However, the pH did not change significantly over the 20-minute period. The PaO2 level after 20 minutes is much lower than before DHCA, it would be well tolerated in normothermic adults. The PO2 level in the brain may be even lower given its high metabolic rate. So measuring arterial PO2 continuously during DHCA may provide a surrogate method for determining maximum safe time under DHCA for adults.

Adult↗
PubMed1997

Myxoma: life-threatening benign nonepithelial tumor of the larynx.

Myxoma is a rare nonepithelial neoplasm of the larynx frequently misdiagnosed as a large vocal polyp due to its slow-growing nature. Myxoma is a benign but often infiltrating neoplasm of uncertain mesenchymal cell origin, characterized by irregular round, spindle or stellate cells within a matrix containing abundant mucoid material, scant vascularity and a variable meshwork of reticulum and collagen. We report one case of myxoma with life-threatening dyspnea requiring tracheotomy.

Critical Illness↗
PubMed1997

Design of a medical image processing software for clinical-PACS.

Software modules for interactive display, manipulation and retrieval of medical images have been designed for a Picture Archiving and Communications System (PACS). The target of these modules is not for a high-end diagnostic workstation for radiologists, but for a PC-based low cost clinical workstation for a referring physician. This software is constructed based on a concept of an object-oriented language which is designed to be modular and expandable. It consists of several functional modules: (a) a communication module for image retrieval, (b) a standard module for the interpretation of the DICOM images, (c) a user interface module for the non-computer oriented clinicians and (d) a tool module for viewing and manipulating images as well as editing the annotation.

Image Processing, Computer-Assisted↗
PubMed1996

Signaling through CD38 augments B cell antigen receptor (BCR) responses and is dependent on BCR expression.

mAbs directed against the ectoenzyme CD38 will induce B cell proliferation in normal resting B lymphocytes, but cannot induce proliferation in B cells that are unresponsive to B cell Ag receptor (BCR) cross-linking. Using the CD38- murine B cell line A20 we have examined the relationship between CD38- and BCR-mediated signaling after transfection of wild-type or mutant CD38 molecules. Although association between CD38 and the BCR was not detectable, co-cross-linking of CD38 and the BCR gave rise to a synergistic response, and expression of CD38 lowered the threshold for BCR-induced responses. Generation of Ig loss variant clones established that coexpression of the BCR was required for CD38-mediated signal transduction. The cytoplasmic tail of Ig alpha or Ig beta rescued CD 38 responsiveness in the CD38+Ig- cells provided that the chimeric molecules were coligated with CD38. Separate experiments indicated that the cytoplasmic tail of CD38 is not required for CD38 signaling. The anti-CD38-induced response was dependent on the influx of extracellular calcium but was not accompanied by detectable tyrosine phosphorylation of any cellular proteins. Together, these data demonstrate that the CD38 molecule can influence BCR-induced responses and that CD38 signaling is dependent on the BCR complex, perhaps to utilize a functional cytoplasmic tail(s) for intracellular signaling.

ADP-ribosyl Cyclase↗
PubMed1996

The specificity of association of the IgD molecule with the accessory proteins BAP31/BAP29 lies in the IgD transmembrane sequence.

Mature B cells co-express on their cell surface two classes of antigen receptor, the IgM and IgD immunoglobulins. The structural and functional differences between the two receptor classes are poorly understood. Recently two proteins of 29 and 31 kDa (BAP29 and BAP31) have been described that are preferentially associated with membrane IgD but only weakly with membrane IgM. We describe here the cloning of full-length murine and human BAP31 cDNAs encoding proteins of 245 and 246 amino acids respectively. The two BAP31 proteins are 95% identical. The BAP31 gene is ubiquitously expressed in murine tissues and is located on the X chromosome in both mouse and man. The murine BAP31 protein has 43% sequence identity to murine BAP29. Both proteins have a hydrophobic N-terminus and an alpha-helical C-terminus which ends with a KKXX motif implicated in vesicular transport. By a mutational analysis we have identified amino acids in the transmembrane sequence of the delta m chain that are critical for binding to BAP31/BAP29. A structural model of the BAPs and their potential functions are discussed.

Amino Acid Sequence↗
PubMed1996

Central hemodynamic changes associated with pregnancy in a long-term cardiac transplant recipient.

We present a case of pregnancy in a woman who had undergone cardiac transplantation and was monitored by "surveillance" biopsy at intervals during the entire course of pregnancy and the immediate postpartum period. Exercise reserve remained normal during these periods despite angiographic evidence of coronary disease and episodes of rejection. Central hemodynamic changes were similar to those expected during normal pregnancy. Hemodynamic stability was maintained during epidural anesthesia for labor and subsequent cesarean section.

Adult↗