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K Linnet

Publications and source records attributed to K Linnet.

76 records · Page 5Linked to original sources
PubMed1982

The patterns of glycine and taurine conjugates of bile acids in serum in hepatobiliary disease.

Fasting serum concentrations of glycine and taurine conjugates of cholic, chenodeoxycholic, and deoxycholic acid were measured with a high-pressure liquid chromatography-enzymatic assay in patients with hepatobiliary disease. The total glycine to taurine ratio was significantly lower in extrahepatic cholestasis (median 1.1) than in cirrhosis (median, 2.0) and controls (median, 1.7). In patients with cirrhosis the ratio was significantly correlated with the S-bilirubins, P-coagulation factors (II + VII + X), and S-total conjugated bile acids. Because of large overlaps of the ratio between the groups the glycine to taurine ratio is of hardly any diagnostic value. The ratio of cholic acid conjugates to chenodeoxycholic acid conjugates was over 1.5 in 10 of 12 cholestasis patients and below this value in all but 1 patient with cirrhosis; the separation of the groups was not improved by splitting the ratio in glycine and taurine conjugates. This study does not suggest that separate determination of glycine and taurine conjugates of bile acids in serum adds diagnostic information in hepatobiliary disease.

Adult↗
PubMed1982

Predictive value of the concentration in serum of total 3 alpha-hydroxy bile acids in the diagnosis of hepatobiliary disease.

Fasting serum levels of total 3 alpha-hydroxy bile acids were measured in 163 consecutive patients, who clinically were suspected of having a hepatobiliary disorder. By means of routine biochemical liver tests and paraclinical investigations the clinical suspicion was confirmed in 95 patients and disproved in the other 68 patients. A comparison between this diagnostic grouping and the serum bile acids levels showed that the predictive value of serum concentrations above a discrimination value of 10 mumol/l--that is, the predictive value of a positive test--was 1.0 (0.94-1.0), whereas the corresponding predictive value of a negative test was 0.66 (0.56-0.75). Thus, fasting serum levels of total 3 alpha-hydroxy bile acids may be used as a highly specific and moderately sensitive diagnostic test for hepatobiliary disorders. Furthermore, the study showed that the nosographic sensitivity of total 3 alpha-hydroxy bile acids in patients with verified cirrhosis was significantly greater than that of serum bilirubins and not significantly different from those of the other routine biochemical liver tests.

Adult↗
PubMed1982

A high-pressure liquid chromatographic-enzymatic assay for glycine and taurine conjugates of cholic, chenodeoxycholic and deoxycholic acid in serum.

An assay for glycine and taurine conjugates of cholic, chenodeoxycholic and deoxycholic acid in serum by a high pressure liquid chromatographic-enzymatic system is presented. The bile acids are extracted from serum by a reverse-phase liquid chromatographic process with an octadecylsilane column. The bile acid conjugates are separated on a muBondapak C18 column with methanol/KH2PO4 buffer, 20 mmol/l, pH 5.3 as a mobile phase in less than 30 min at a flow rate of 1.4 ml/min. The bile acid fractions are measured by enzymatic fluorometry using a 3 alpha-hydroxysteroid dehydrogenase-diaphorase system. Recoveries ranged from 82 to 96%, coefficients of variation were from 5 to 15%, and detection limits were from 0.03 to 0.08 mumol/l. Mean serum concentrations ranged from 0.10 to 0.39 mumol/l in the fasting state and from 0.29 to 1.55 mumol/l postprandially in subjects with normal liver function.

Adult↗