Biomedical subjects

K L Wu

Publications and source records attributed to K L Wu.

34 records · Page 2Linked to original sources

PubMed1994

Morphological study of fetal neocortical transplant grafted to the cerebral corresponding area in young rats. Differentiation of immature neurons and reciprocal connections of fibers between graft-host brain.

The fetal neocortical transplant (E15-17 days gestation) of Wistar rat was grafted to the corresponding neocortical region (frontal-parietal lobe) of the same strain in young rats (4-5 weeks old). On the 7th, 15th, 30th, 60th, 150th day after transplantation, the sections cut through the middle area of graft-host brain were examined by HE, Nissl, Glees stain, immunohistochemical technique for GFAP and NF, Nissl, Glees stain, immunohistochemical technique for GFAP and NF, acetylcholinesterase (AChE) histochemistry as well as horseradish peroxidase (HRP) retrograde tracing with light microscope. Some of the sections were also examined with TEM. The result showed that most immature neurons within the graft can survive, grow, differentiate and mature, and are similar to the structure of the neocortical neurons of host brain. This study also provides patterns of integration of the interface between graft-host brain varying with the proliferation of reactive astrocyte as well as graft-host reciprocal connection of fibers.

Acetylcholinesterase↗

PubMed1993

[Comparison of the transmission dynamics and the control effects between malaria and filariasis by using mathematical model].

By dynamic modeling based on Ross & MacDonald's mathematical model, the characteristics of rapid transmission of malaria and slow transmission of filariasis was compared. The dynamic mechanism showed that the infection efficiency in filariasis, namely, the probability of becoming infected in man by one infective bite of mosquito, was much lower than that in malaria; hence the vectorial capacity or transmission velocity in filariasis was also markedly lower than that in malaria. Since the intensity of infection i.e. the microfilaria density can largely affect the infection efficiency in filariasis, drug treatment, especially using DEC-medicated salt can reduce the intensity of infection and the infection efficiency, thus interrupting transmission finally. However, for malaria, only when the measures for mosquito vector control (including mosquito proofing) are taken as a priority to reduce the vectorial capacity or transmission velocity, malaria can then be controlled subsequently. These theoretical analyses are being demonstrated by the practice for malaria and filariasis control in our country, which could also be used as a theoretical base for enlightening the successful filariasis control strategies in our country.

Animals↗

PubMed1993

Primary carcinoma of the gallbladder: a review of 10 years of experience at Tri-Service General Hospital.

Thirty-two cases of primary carcinoma of the gallbladder proven by surgery and pathological biopsy between January 1982 and June 1991 at the Tri-Service General Hospital, Taipei, were analyzed retrospectively. There were 16 male and 16 female patients with a mean age of 66.1 years. The most common clinical manifestations were right upper quadrant abdominal pain and poor appetite. The most common laboratory finding was an elevation of alkaline phosphatase. The preoperative diagnostic rate of this series was 46.9% (15/32 cases), through use of abdominal sonography, computed tomography, endoscopic retrograde cholangiopancreatography and celiac angiography. The coexisting gallstone incidence was 65.6% and the resectability rate, 59.4%. The histological classifications were adenocarcinoma with variable differentiation in 31 cases, and undifferentiated adenocarcinoma in one. The liver was the most common site for metastasis (53.1%), followed by lymph nodes at porta hepatis (21.9%), omentum (12.5%), peritoneum (9.4%), lung (6.3%), colon (3.1%) and duodenum (3.1%). According to the Nevin's staging system, three patients were in stage I and all survived more than five years. Of the two patients in stage II, one survived longer than five years and the other survived longer than seven months. There were three cases in stage III: one patient died of metastasis eight months postoperatively, while the other two cases lived for seven and nine and a half months respectively. There were 24 cases in stage IV and stage V, all of them died less than six months after diagnosis. Poor prognosis for patients with primary carcinoma of the gallbladder makes early diagnosis and treatment important.

Adenocarcinoma↗

PubMed1992

An experimental study of trachea anastomosis in rabbits using carbon dioxide laser.

A technique of sutureless trachea anastomosis in rabbits using carbon dioxide laser was reported herein. In 6 rabbits with laser-assisted trachea anastomoses, only one was found to have slight anastomotic stenosis after operation, whereas 6 rabbits with conventional sutures all were found to have anastomotic stenosis. Results show that laser-assisted trachea anastomosis has certain advantages over the conventional suturing technique, thereby finding wide application in clinical tracheal reconstruction and lung transplantation.

Anastomosis, Surgical↗

PubMed1991

Morphometric studies of parietal cells in cats before and after lesser-curvature seromyotomy.

A modified, highly selective vagotomy-seromyotomy of the lesser curvature of the stomach was performed on five groups of cats. The horseradish peroxidase (HRP) tract-tracing method was used to detect the regeneration or reinnervation of vagal nerve branches. Morphological changes to the parietal cells and to the gastric mucosa were also examined by light and electron microscopy. Following surgery, the cats were sacrificed at the fourth, eighth, twelfth, sixteenth and the twentieth week. At the sixteenth week, partial regeneration of vagal nerve branches was found. Between the fourth and the twelfth week there was a significant increase in the number of parietal cells per 0.1 mm-wide of mucosa column and in the volume fraction of the mucosa made up of parietal cells. Of the four types of parietal cells, "stimulated", "partially stimulated", "returning" and "resting", the resting type was predominant after seromyotomy, especially between the fourth and the twelfth week. Based on the above observation, we concluded that the modified lesser-curvature seromyotomy depresses the function and responsiveness of the parietal cells despite an increase in their number and in their volume fraction.

Animals↗

PubMed1989

High energy laser irradiation and electric surgery for obstructive gastrointestinal cancer.

A total of 35 patients with advanced obstructive gastrointestinal cancer received monopolar electrosurgery and Nd: YAG laser therapy from January 1985 to June 1988. These included 13 cases of esophageal cancer (U/3 6, M/3 2, L/3 5), 14 case of gastric cancer (cardia 7, corpus 3, antrum 4), and 8 cases of rectal cancer. Twenty (esophageal 9, stomach 8, rectal 3) of 35 patients had much improved results, with the size of the lesion reduced over 90%, and the endoscope could smoothly pass through the stenotic area after electrosurgery or laser therapy. The other 12 patients had partially improved results with the tumor size being reduced 50-90% and the patients could take semiliquid food easily. Both groups of much improved or partial improved had a statistically significant difference (P less than 0.01) compared to the group of failures to treatment. Two patients developed bleeding after treatment. Twenty-one patients died of causes other than complications of electrosurgery or laser therapy. The average survival was 6.8 months. Nd: YAG laser and electrosurgery can provide good palliative results and contribute to a good quality of life in advanced obstructive gastrointestinal cancer.

Adult↗

PubMed1989

A clinical controlled trial of endoscopic sclerotherapy for repeated esophageal variceal bleeding.

Thirty-seven patients with postnecrotic cirrhosis of the liver and 13 patients with primary hepatoma were proven to have repeated bleeding from ruptured esophageal varices. Clinically controlled trials were performed by assigning patients to either sclerotherapy or control arms (25 patients each). Combined intra-variceal and para-variceal injection before an upper endoscopic examination was performed in the sclerotherapy group. In all 25 sclerotherapy cases (100%) hemostasis was successful, which was a statistically significant success rate compared to the control group (52.0%) (p less than 0.01). In the sclerotherapy group 20% (5/25 cases) developed rebleeding, which was less than the 48.0% (7 cases of continuous bleeding and 5 cases of rebleeding) of the control group (p less than 0.05). Four cases (16.0%) in the sclerotherapy group died of erosive gastritis with massive bleeding, compared to 8 fatalities (32.0%) in the control group, because of uncontrolled esophageal variceal bleeding. Endoscopic sclerotherapy is a very effective method for arresting bleeding esophageal varices, and for decreasing the rebleeding rate.

Adult↗

PubMed1988