Search PubMed⌕ Search

Biomedical subjects

K L Smith

Publications and source records attributed to K L Smith.

At least 145 records · Page 8Linked to original sources

Late functional outcome in patients with tibia fractures covered with free muscle flaps.

The functional outcome and work capacity of patients treated with a free muscle flap to cover open grade III tibial fractures was assessed. The conditions of patients, eight with grade IIIB and six with grade IIIC isolated open tibia fractures, treated with a free muscle flap transfer less than 3 months after their injury, were retrospectively reviewed. Flap survival was 86%. Twelve of the 14 were contacted, with follow-up time averaging 7 years. Four of the 14 eventually had below-knee amputations and one of the 10 patients with a successful limb salvage died of unrelated causes. All nine surviving patients with salvaged limbs had healed fractures in an average of 15 months (range, 8-23). Six were initially infected, but drainage had stopped an average of 13.5 months after flap coverage. No wounds were draining at last follow-up observation. Those tibias that were initially infected have been drainage free for an average of 78 months. The average total hospital cost of reconstruction was $48,996.40. The functional outcome in 12 patients was assessed. Eight of the nine patients whose limbs were salvaged returned to work, six to jobs with demands similar to their preinjury occupation. Three of the four patients with limb amputations were also able to return to jobs similar to their preinjury occupation. Patients must be made aware of the expected course of reconstruction and anticipated final outcome. Despite rarely achieving normal function, returning to work is a reasonable goal.

Adult↗

PCR detection of cytomegalovirus: a review.

CMV is of major concern in immunocompromised and immunosuppressed patients. Since CMV can be transmitted by leucocyte transfusions from healthy seropositive donors (Hirsch, 1984) it has been stated that leucocytes are the natural reservoir of latent CMV (Jiwa et al, 1989). Although tissue culture is the method currently used for the diagnosis of CMV infection, this technique is time consuming, expensive and does not detect latent virus. As 80% of normal Australian blood donors are seropositive for CMV (Ho, 1990) the amount of blood available for high risk patients is greatly reduced. The dramatic gains in sensitivity of viral detection made possible by the PCR technique offers new hope for the detection of otherwise elusive latent genomes as well as more routine application in the detection of viraemia or other active infection. However, for this technique to be adopted by clinical laboratories it must be shown to be easily reproducible and cost-effective. Thus, the PCR may have an important role in the development of CMV-negative blood products, as well as being a powerful test in diagnostic virology. It is expected that it will reduce the morbidity and mortality rate in susceptible patients at risk of CMV when given transfusions of blood from subjects who may carry the virus.

Cytomegalovirus↗

Removal of cytomegalovirus DNA from donor blood by filtration.

Blood from five donors, previously shown to be positive for cytomegalovirus (CMV) DNA following polymerase chain reaction (PCR) amplification, was filtered through commercially available leucocyte filters. Analysis of pre- and post-filtration samples by PCR with ethidium bromide staining has shown that filtration was successful in removing CMV DNA from all samples. This is evidence that leucocyte filtration of red cell concentrates may greatly decrease the risk of CMV disease following transfusion to susceptible patients.

Base Sequence↗

Renin and renin inhibition in anephric man.

Renin activity appears to be present in low concentrations in the plasma of anephric humans but could be artifactual secondary to inadvertent activation of prorenin during specimen collection and handling or from a renin-like enzyme. We studied the effects of specimen collection, storage, different assay conditions, trypsin activation, and the renin inhibitor EMD 56133 (E Merck, Darmstadt) on plasma renin activity (PRA) in anephric man. PRA was detectable in all seven bilaterally nephrectomized (BNX) patients (0.2 +/- 0.1 ng AI/ml/hr, range 0.1-0.7) but was significantly lower than normals (2.4 +/- 0.3 ng AI/ml/hr, range 1.5-3.1, p = 0.001). PRA was not different in BNX whether blood samples were collected on ice or at room temperature and assayed immediately or whether samples were frozen and assayed several days later. Prolonged cold storage of samples and five freeze-thaw cycles over six to seven months did not significantly increase PRA in normals or anephrics. However, deliberate repeated freezing and thawing over the period of a single day increased PRA 4.1-fold in BNX and 1.6-fold in normals. Renin-like activity was also detected in BNX individuals using renin concentration determinations with either excess human or sheep angiotensinogen. The inhibition of renin activity (IC-50% = 3.16 x 10(-9) molar) by EMD 56133 was not different between BNX and normals. Thus, active renin is present in the plasma of anephric humans and does not result from the inadvertent activation of prorenin due to sample handling. Although the source of PRA in BNX is unknown, the enzyme appears functionally normal as evidenced by the dose-response to a single renin inhibitor.

Adult↗

Vitamin E as an adjuvant in an Escherichia coli J5 vaccine.

Vitamin E was tested as an adjuvant in an Escherichia coli (O111:B4) J5 vaccine. Twenty cows were assigned to five groups of 4 cows. Cows in four groups were vaccinated with an E. coli J5 bacterin containing 5 ml of 10(9) boiled cells/ml. Vaccinations were at drying off, 30 d after drying off, and within 48 h after calving. Vaccine adjuvants differed among groups. The four treatment adjuvants were 5 ml of Freund's incomplete adjuvant, 5 ml of vitamin E, 2.5 ml of Freund's plus 2.5 ml of vitamin E, and 5 ml of PBS. Cows in the fifth group were unimmunized controls. A front mammary quarter of each cow was challenged by infusion of 10 micrograms of E. coli J5 lipopolysaccharide approximately 4 wk into lactation. Vitamin E alone enhanced serum IgM titers but had no effect on milk IgM or serum and milk IgG titers. The mixture of Freund's plus vitamin E resulted in peak IgG titers in serum and milk comparable with that of Freund's alone. Persistency of IgG titers in cows immunized with the Freund's plus vitamin E mixture was greater than the persistency of titers for cows immunized with the vaccine containing Freund's alone as the adjuvant. The mixture of Freund's plus vitamin E had a synergistic effect in reducing severity of systemic clinical signs.

Adjuvants, Immunologic↗

Growth responses of coliform bacteria to recombinant bovine cytokines.

Growth responses of 10 coliform isolates to recombinant bovine cytokines were measured in vitro. Six Escherichia coli and four Klebsiella pneumoniae isolates obtained from bovine IMI were tested for growth responses to recombinant bovine interleukin-1 beta, interleukin-2, and interferon-gamma. Cytokines were tested at 10(4), 10(3), 10(2), and 10 U/ml of media. Media used were a synthetic tissue culture medium, a chemically defined synthetic bacterial growth medium, and UHT sterilized milk. Bacterial counts in the synthetic tissue culture medium and UHT milk increased slightly as concentration of interferon-gamma in the media increased. Recombinant bovine interferon-gamma increased bacterial populations during the log growth phase but did not affect the number of bacteria in stationary growth phase. Bacterial growth responses were not related to either interleukin-2 or interleukin-1 beta concentrations in any of the three media. Bacterial growth responses to cytokines were not related to differences in either serum susceptibility, growth of isolates in dry cow secretion, duration of IMI from which isolates were obtained, or bacterial species.

Animals↗

Role of vitamin E and selenium in host defense against mastitis.

Vitamin E and Se are essential nutrients that share common biological activities. Deficiencies in either of these micronutrients have been related in increased incidence and severity of mastitis. A known physiological consequence of alpha-tocopherol or Se deficiency is reduced neutrophil activity. Vitamin E and the Se-containing enzyme, glutathione peroxidase, and antioxidants that protect neutrophils from the destructive action of toxic oxygen molecules necessary for intracellular kill of ingested pathogens. Dietary supplementation of cattle with Se results in a more rapid neutrophil influx into milk following intramammary bacterial challenge and increased intracellular kill of ingested bacteria by neutrophils. Dietary supplementation of early lactation cows with vitamin E results in increased bactericidal activity by bovine blood neutrophils. Recently completed trials have shown that subcutaneous injections of vitamin E approximately 10 and 5 d prior to calving successfully elevated neutrophil alpha-tocopherol concentrations during the periparturient period and negated the suppressed intracellular kill of bacteria by neutrophils that is commonly observed at calving.

Animals↗

Localized excitatory synaptic interactions mediate the sustained depolarization of electrographic seizures in developing hippocampus.

Repetitive synchronized neuronal discharging that lasts for seconds and even minutes in in vitro brain slice preparations are important new models in experimental epilepsy. In hippocampal slices from 1-2-week-old rats, individual CA3 pyramidal cells undergo a sustained depolarization during such electrographic seizures, induced by GABAA receptor antagonists. In experiments reported here these events were produced in small isolated segments of the CA3 subfield, measuring only 400-500 microns along the cell body layer. In such minisclices local application of either kynurenic acid or 6-cyano-7-nitroquinoxaline-2-3-dione (CNQX) to the proximal basilar dendrites abolished the synchronized discharges of electrographic seizures. Interictal spikes appeared unaffected by this treatment. Application of these excitatory amino acid receptor antagonists to distal basilar dendrites or apical dendrites was ineffective. In "larger" minislices, measuring 700-1000 microns along the cell body layer, application of kynurenic acid, CNQX, or TTX to the proximal basilar dendrites did not abolish electrographic seizures but instead selectively suppressed the intracellularly recorded sustained depolarization and the coincident slow negative field potential recorded in proximal basilar dendrites. Results of several experiments suggest that electrographic seizures recorded under these conditions were produced by a remote network of "generator cells." Since the remote neurons were unaffected by local application of the drugs, it seemed likely that they continued to undergo a sustained depolarization. Simultaneous blockade of basilar dendritic synapses in the "generator" population abolished electrographic seizures throughout these larger minislices. These results suggest that the sustained depolarization plays a central role in seizure generation and that it does not have to be generated in every neuron, only in a critical number of "generator cells" for a seizure to occur. Taken together, results presented here suggest that the sustained depolarization of electrographic seizures is a separate physiological process from the more rapid repetitive depolarizations of the seizure discharges and is required if electrographic seizures are to occur. This slow depolarization appears to be synaptically mediated and generated exclusively in proximal basilar dendrites. Therefore, in addition to the excitatory synaptic potentials involved in paroxysmal depolarization shift generation, a second form of recurrent excitation may exist in immature hippocampus. Not only is this physiological process critical for the genesis of seizures, but it also appears to be highly partitioned within the hippocampal laminae.

6-Cyano-7-nitroquinoxaline-2,3-dione↗

Interspecific conjugal transfer of antibiotic resistance among staphylococci isolated from the bovine mammary gland.

In vitro transferability of penicillin, streptomycin, tetracycline, and erythromycin resistance from coagulase-negative staphylococci to Staphylococcus aureus and among the former species of bovine mammary gland origin was examined by bacterial mating on filters and by mixed-culture matings in broth and in skim milk. One hundred twenty-six (42 each on filter, in broth, and in skim milk) matings were performed among 37 isolates of different Staphylococcus species. Transfer of resistance to penicillin, tetracycline, or erythromycin was not detected. Of 51 matings performed to determine streptomycin-resistance transfer, 9 (3 each on filters, in broth, and skim milk) were successful. Nine strains representing 3 species of coagulase-negative staphylococci were tested as prospective donors of streptomycin resistance. Of these, 2 strains could transfer streptomycin resistance. A double-resistant donor, S hominis, not only transferred its streptomycin resistance to an S chromogenes strain lacking resistance, but also to an S aureus strain already carrying penicillin and tetracycline resistance. The transfer of streptomycin resistance from the donor S hominis, harboring 2 plasmids, to a plasmidless S chromogenes recipient strain was associated with apparent acquisition of the smaller plasmid of the donor by the recipient. The single-resistant donor, S epidermidis 681A, transferred streptomycin resistance to a tetracycline-resistant S aureus recipient. This strain however, failed to transfer its streptomycin resistance to another S aureus, 2 S hyicus, and 1 S xylosus recipient. Frequency of transfer of streptomycin resistance ranged from 1.1 x 10(-5) to 1 x 10(-4). When transfer of resistance was successful, attempts were made to characterize the transfer process.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Determination of conditions for optimum resolution of a high angle thin window energy dispersive spectrometer.

The energy resolution of an energy dispersive spectrometer (EDS) equipped with an ultrathin window (UTW) and mounted at a high take-off angle (72 degrees) on a transmission electron microscope has been studied under a variety of operating conditions. The spectrometer resolution is close to that specified by the manufacturer, up to count rates of 400 cps. Above 400 cps the resolution deteriorates rapidly, and the MCA dead time and zero width increase. Above 10 keV, the height of the background is much greater than expected for bremsstrahlung and shows the shape which has previously been attributed to backscattered electron flux into the detector. It is postulated that the deterioration in resolution with count rate is caused by backscattered electrons reaching the detector through the UTW.

Electron Probe Microanalysis↗

Lateral band translocation for swan-neck deformity.

Lateral band translocation involves transfer of the dorsally subluxed radial lateral band to the palmar aspect of the joint where it is maintained by a sling created between the flexor superficialis tendon and the palmar plate. Thirty fingers with swan-neck deformity of differing causes and a preoperative hyperextension deformity averaging 16 degrees were treated. After operation all swan-neck deformities were corrected and the average flexion contracture was 11 degrees. The range of preoperative flexion was either regained or improved. There were no recurrences and no complications. This relatively simple procedure is an effective treatment of swan-neck deformity and an attractive alternative to previously described procedures.

Female↗

Displacement of an uncemented acetabular component after dislocation of a total hip prosthesis. A case report.

Review of the literature reveals few reports of complications encountered with noncemented acetabular components; most concern problems with screw or cup placement, component wear or migration, or disassembly of modular components. No reports involving the displacement of a noncemented acetabular component were found. This is a case report of a patient in whom a noncemented acetabular component was dislodged after the closed reduction of a dislocated total hip prosthesis 4.5 weeks after surgery. In light of this case, the authors believe these reductions should be performed under general anesthesia with fluoroscopic guidance. Care must be taken at surgery to ream sufficiently and obtain proper cup fit and position. Finally, the authors recommend bicortical screw fixation to provide maximum contact and rigid fixation in the early postoperative period.

Acetabulum↗

Prevention of human rotavirus-induced diarrhea in gnotobiotic piglets using bovine antibody.

The efficacy of passively administered bovine antibody for preventing human rotavirus (HRV)-induced diarrhea was investigated using a gnotobiotic pig model. Cows were immunized with inactivated HRV serotypes 1 (Wa) and 2 (S2) and simian rotavirus serotype 3 (SA11), and immune colostrum and milk were collected. Antibody concentrates derived from these materials were fed to germ-free piglets that were subsequently inoculated with HRV Wa. Both viral shedding and diarrhea were effectively reduced or eliminated in a dose-dependent manner as a result of HRV immune antibody feeding. A quantitative virus-neutralizing (VN) antibody method permitted assessment of the functional antibody dose required to achieve a 50% reduction of disease (PD50). PD50 dose levels of 15.8 and 19.5 x 10(6) VN antibody units were determined for inhibition of diarrhea and viral shedding, respectively. Studies reported here provide new information on the quantitative relationship between protective antibody dose and diarrheal disease response.

Animals↗

Observations on the inhibition of serum and cell surface enzymes by eicosapentaenoic acid.

The relationship between serum and tumour cell surface proteolytic enzymes and the development of muscle breakdown in cancer cachexia has been studied in a murine model of the condition (MAC16). The surface of the MAC16 tumour cells carried a proteolytic enzyme referred to as guanidinobenzoatase (GB). Serum from mice also contained an enzyme (referred to as MSE) which cleaved the trypsin inhibitor 4-methylumbelliferyl-p-guanidinobenzoate as a true substrate, but there was no relationship with weight loss or the presence or absence of tumour and the level of this serum enzyme. Polyunsaturated fatty acids (PUFAs) were shown to be inhibitors of MSE at microM concentrations and one PUFA, eicosapentaenoic acid (EPA) was found to be a non-competitive inhibitor of both MSE and GB. The effect of EPA was specific since other proteolytic enzymes, trypsin, esterase and tissue plasminogen activator were unaffected by concentrations inhibiting GB and MSE. MSE and GB are two different enzymes which possess some common properties. However, GB is likely to be significant for tumour development since MSE is also found in normal mouse serum.

Animals↗

Opsonic activity of bovine serum and mammary secretion after Escherichia coli J5 vaccination.

Six pairs of cows were used to determine the effects of immunization with an Escherichia coli (O111:B4) J5 bacterin on in vitro opsonization of a smooth heterologous strain of E. coli. One cow in each pair was either immunized with the vaccine or sham-immunized at drying off, 30 d after drying off, and at calving. Opsonizing bacteria with serum collected from vaccinated cows 21 d after calving resulted in higher mean number of intracellular bacteria per phagocytosing neutrophil than opsonizing bacteria with serum collected from control cows. Phagocytic parameters using serum collected at drying off and calving did not differ between treatment groups. A trend for enhanced opsonic activity of colostrum from vaccinates was noted. Enhanced opsonization by serum from vaccinated cows coincided with higher serum IgM titer to E. coli J5 whole cell antigen compared with controls. Serum IgG titers to E. coli J5 did not differ between groups. Colostrum IgG titers to E. coli J5 were greater at calving in vaccinated than in control cows. Colostrum and milk collected 21 d after calving from vaccinated cows had higher IgM titers to E. coli J5 than did mammary secretions from control cows. Numbers of intracellular bacteria per phagocytizing neutrophil were correlated positively with IgM titers to E. coli J5 in both serum and colostrum.

Animals↗

Field trial to determine efficacy of an Escherichia coli J5 mastitis vaccine.

Efficacy of an Escherichia coli (O111:B4) J5 bacterin for preventing naturally occurring IMI and clinical mastitis was tested in a 2.5-yr field trial in a 225-cow commercial herd. Cows with odd-numbered identification were vaccinated, and cows with even-numbered identification served as unvaccinated controls for each lactation during the study. Immunizations were subcutaneous on the upper part of the rib cage just posterior to the scapula at drying off, 30 d after drying off, and at calving. Percentage of quarters infected at calving with Gram-negative bacteria did not differ between treatment groups. A total of 67% of Gram-negative bacterial IMI present at calving in control cows became clinical during the first 90 d of lactation compared with 20% in vaccinated cows. Rate of Gram-negative bacterial clinical mastitis was higher in control cows than in vaccinated cows during the first 90 d of lactation. Immunization with the E. coli J5 bacterin did not reduce level of Gram-negative bacterial IMI at calving but did reduce incidence of clinical mastitis.

Animals↗