Search PubMed⌕ Search

Biomedical subjects

K L Smith

Publications and source records attributed to K L Smith.

At least 253 records · Page 14Linked to original sources

Plastics on the Sargasso sea surface.

Plastic particles, in concentrations averaging 3500 pieces and 290 grams per square kilometer, are widespread in the western Sargasso Sea. Pieces are brittle, apparently due to the weathering of the plasticizers, and many are in a pellet shape about 0.25 to 0.5 centimeters in diameter. The particles are surfaces for the attachment of diatoms and hydroids. Increasing production of plastics, combined with present waste-disposal practices, will undoubtedly lead to increases in the concentration of these particles. Plastics could be a source of some of the polychlorinated biphenyls recently observed in oceanic organisms.

Bacteria↗

Saphylococcal beta-hemolysin. I. Purification of beta-hemolysin.

The purification of staphylococcal beta-hemolysin was accomplished by the successive use of three protein fractionation methods. The first method employed was a double precipitation with the use of ammonium sulfate at 65% saturation. The second phase of purification used Sephadex G-100 column fractionation. The third phase utilized either carboxymethyl cellulose or diethylaminoethyl cellulose fractionation. The last two fractionation methods both resulted in the separation of a relatively high concentration of cationic hot-cold lysin and a low concentration of anionic hot-cold lysin. Because of the low concentration of the anionic component, its purity could not be assessed. However, the purity of the cationic component was demonstrated by immunodiffusion, microimmunoelectrophoresis, and by disc polyacrylamide gel electrophoresis. In addition, antisera against purified cationic beta-hemolysin yielded one line of precipitate when tested against the original crude beta-hemolysin. The purified cationic beta-hemolysin was stable in the lyophilized state. Crude beta-hemolysin was dermonecrotic, whereas purified cationic beta-hemolysin was not dermonecrotic even after Mg(++) activation.

Animals↗

Effect of joint position and ligament tension on the MR signal intensity of the cruciate ligaments of the knee.

Six cadaveric lower extremities were imaged with T1-weighted spin-echo pulse sequences with the knees extended and flexed to 90 degrees. Magnetic resonance signal intensities of the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) were compared. Changing from extension to flexion resulted in decreased signal intensity in six of six ACLs and five of six PCLs. Two of the knees were then imaged with and without tension applied to the ACL. Both specimens showed a decrease in signal intensity with tension, followed by an increase in signal intensity with release of the tension. Finally, in three of the limbs the ACL was surgically reconstructed and then imaged with and without tension applied to the tendon graft. Signal intensity decreased with tension and increased with release of the tension in all three specimens. Thus, joint position and changes in ligament tension affect the signal intensity of the ACL and PCL, generally resulting in a signal intensity decrease with tension.

Aged↗

Bacterial mutagenicity tests on 4-chloromethylbiphenyl and 2 structural analogues.

4CMB, 4HMB and BC were tested in 5 strains of S. typhimurium and 2 strains of E. Coli without S9. 4HMB was negative in all strains. 4CMB was a strong positive mutagen in TA1535, TA1538, TA98, TA100 and WP2uvrA-(pKM101), and BC was a weak mutagen in TA100 and WP2uvrA-(pKM101). Positivity was determined as a dose response over 3 or more points, in repeat experiments, giving a significant correlation coefficient.

Benzyl Compounds↗

Metaphase analysis of human lymphocytes treated with 4-chloromethylbiphenyl and benzyl chloride.

4CMB and BC caused mitotic inhibition in human lymphocytes at low doses (greater than 20 and greater than 10 microgram/ml respectively). At non-inhibitory concentrations, neither substance produced increases in cells with breaks or breaks per cell, although 4CMB did induce a significant increase in gaps at dose. 4CMB and BC are not chromosome mutagens in this system.

Benzyl Compounds↗

Sister-chromatid exchanges in human lymphocytes treated with 4-chloromethylbiphenyl and benzyl chloride.

At non-inhibitory concentrations, 4CMB and BC both induced small, but dose-related increases in SCE. The dose-response curves gave a highly significant correlation for 4CMB (r = 0.996, P less than 0.001) and a significant correlation for BC (r = 0.757, P less than 0.05) which reflects the relative activities of these substances in bacterial mutation tests.

Benzyl Compounds↗

Computer-delivered modeling of exposure for spider phobia: relevant versus irrelevant exposure.

Spider phobic subjects (n = 45) completed 3 x 40 minute computer-delivered treatment sessions. Questionnaire ratings of phobic severity were completed pre- and posttreatment (n = 45) and 6-12 month follow-up (n = 38). The program used interactive animations to model self-exposure treatment methods. Subjects were randomized to one of three treatment groups (n = 15), each receiving a different version of the program. These treatment conditions were relevant exposure with feedback (REF), relevant exposure with no feedback (RENoF), or irrelevant exposure with feedback (IEF). Relevant exposure modeled exposure to spiders, irrelevant exposure to elevators. All groups showed significant phobic improvement following the treatment, as measured on a variety of instruments. Learning on the programs was demonstrated by a significantly increased performance (time taken to reach a target score) within each group across the three computer treatment sessions. Outcome was not significantly affected either relevance of exposure or the manipulation of the onscreen feedback. Subjects' report of exposure treatment undertaken at home correlated positively with phobic improvement.

Adult↗

Tissue repair of the epidermis and dermis.

Wounds of the skin heal in a predictable fashion and at a fairly constant rate if impediments to healing can be avoided. For the skin wound of the hand, the therapist and surgeon face paradoxic demands to balance optimal immobilization for wound healing with optimal motion for function. As long as the wound remains stable, therapy can begin very early in the postoperative period without interruption of the wound-healing process.

Bandages↗

Posteroinferior glenoplasty can change glenoid shape and increase the mechanical stability of the shoulder.

The treatment of recurrent posterior glenohumeral instability remains an unsolved clinical problem. Although various types of capsulorraphy have been advocated, outcome studies indicate that it is difficult to achieve a balance between stability and mobility. Alterations of the bony glenoid for posterior instability have been proposed, but are not well understood from a mechanical perspective. This investigation had 2 purposes: (1) to determine in a cadaver model if posteroinferior glenoplasty can change the shape of the glenoid, and (2) to determine if altering the shape of the glenoid can increase the mechanical stability of the glenohumeral joint. We determined the effective glenoid shape in 7 normal cadaver glenoids by tracking the path of the center of the humeral head as it was translated across the glenoid face in 8 different directions. These determinations enabled us to calculate the maximum effective slope of the glenoid in each direction. We then determined the mechanical stability of the glenoids in each of the 8 directions by measuring the tangential force required to dislocate the shoulder under a 50-N compressive load. The ratio of the dislocating force to the compressive load was defined as the stability ratio. All measurements were repeated after a standardized posteroinferior glenoplasty was performed. Posteroinferior glenoplasty increased the posteroinferior glenoid depth from 3.8 +/- 0.6 mm to 7.0 +/- 1.8 mm and shifted the center of the humeral head an average of 2.2 mm anteriorly and 1.8 mm superiorly. These changes in dimension could be directly visualized as an immediate mechanical consequence of the glenoplasty procedure, particularly because of the insertion of the bone wedge. Glenoplasty increased the posteroinferior glenoid slope from 0.55 +/- 0.07 to 0.83 +/- 0.12 and increased the posteroinferior stability ratio from 0.47 +/- 0.10 to 0.81 +/- 0.17. This is a more than 70% increase in the tangential force that can be resisted before dislocation. The increase can be quantitatively understood as a direct mechanical consequence of the altered shape of the glenoid concavity. These numbers indicate that, in this cadaveric model, posteroinferior glenoplasty results in defined changes in the effective glenoid shape and in the mechanical stability of the glenohumeral joint. However, this study does not establish the role of this procedure in the clinical management of posterior glenohumeral instability.

Biomechanical Phenomena↗