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K Kushida

Publications and source records attributed to K Kushida.

147 records · Page 9Linked to original sources
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The effect of aging and osteoarthritis on the mature and senescent cross-links of collagen in human meniscus.

Pyridinoline and deoxypyridinoline are mature cross-links maintaining the structure of collagen, whereas pentosidine is a senescent cross-link that increases with age. The aim of this study was to investigate the effect of aging and osteoarthritis on these cross-links in human meniscus. Meniscus was obtained from 21 patients with osteoarthritis and 26 nonarthritic trauma patients. Hydroxyproline was measured in hydrolyzed samples. The amount of pyridinoline, deoxypyridinoline, and pentosidine cross-links measured in the hydrolyzed samples using a fluorescent high-performance liquid chromatography technique was expressed per hydroxyproline. There was no correlation between hydroxyproline, pyridinoline, or deoxypyridinoline and age, but pentosidine exponentially increased with age. There was no difference in hydroxyproline nor cross-links between the peripheral and central portions of the meniscus. There was no significant difference in hydroxyproline between osteoarthritis and nonarthritis. Pyridinoline and deoxypyridinoline in osteoarthritis were significantly lower than in nonarthritis. Pentosidine in osteoarthritis was somewhat higher than that in nonarthritis, but not significantly so. The increase of pentosidine with aging may relate to age-related degeneration of meniscus. In osteoarthritis, the decrease of pyridinoline and deoxypyridinoline may cause and progress the degeneration of meniscus attributable to osteoarthritis.

Adult↗
PubMedDate not supplied

Discrimination ability of pyridinoline crosslinks related markers for bone resorption in postmenopause and osteoporosis.

We investigated the discrimination ability of pyridinolines crosslinks related bone resorption markers. Amino-terminal pyridinolines crosslinked telopeptides (NTx) and carboxy-terminal pyridinolines crosslinked telopeptides (ICTP), pyridinoline (Pyr) and deoxypyridinoline (Dpyr) were measured in 62 premenopausal (PRE), 30 early postmenopausal healthy subjects (POST) and 24 vertebral osteoporosis patients (VX). NTx, Pyr and Dpyr was significantly higher in POST than in PRE. ICTP was not changed in POST compared with PRE. NTx, ICTP, Pyr and Dpyr were significantly higher in VX than in PRE and POST. To evaluate discriminatory ability of four markers, we calculated t-scores, and generated receiver operating characteristic (ROC) curves. In POST, NTx, Pyr and Dpyr had a moderate t-score, 1.8, 1.4 and 1.3, respectively. In ICTP t-score was only -0.2. In VX compared with POST, ICTP, Pyr and Dpyr had a high t-score, 5.9, 7.9 and 7.5, respectively, but t-score of NTx was moderate, 3.6. The areas under ROC curves for NTx, ICTP, Pyr and Dpyr in POST were 75.8%, 33.8%, 78.1% and 79.5%, respectively. In VX, compared with POST, those for NTx, ICTP, Pyr and Dpyr were 94.0%, 86.0%, 97.4% and 95.1%, respectively. In conclusion, NTx performed well in early postmenopausal status, but did not in osteoporosis. ICTP did not have discrimination power in postmenopause but performed well in osteoporosis. Pyr, Dpyr performed moderately well in postmenopause and performed well in osteoporosis.

Adult↗