Biomedical subjects

K Kuroyanagi

Publications and source records attributed to K Kuroyanagi.

At least 55 records · Page 3Linked to original sources

PubMed1994

[Reproductive and developmental toxicity studies of paclitaxel. (III)--Intravenous administration to rats during the perinatal and lactation periods].

Paclitaxel, an antineoplastic agent, was administered intravenously to pregnant Crj: CD (SD) rats daily at dose levels of 0 (saline and vehicle), 0.1, 0.3 and 1.0 mg/kg from day 17 of gestation to postpartum day 21. Results were as follows: 1. The vehicle-treated group had no effect in any of the parameters that were measured in this study when compared to the saline-treated group. 2. 1.0 mg/kg paclitaxel caused suppression of the body weight gains associated with the decreased food consumption in F0 dams during the lactation period. 3. Thymic, heart and uterine weights were reduced in 1.0 mg/kg F0 dams at completion of the lactation period. In addition, thymic atrophy was observed for 1.0 mg/kg F0 dams macroscopically. 4. Paclitaxel did not alter the delivery status of F0 dams or birth, viability and weaning indices in F1 pups. 5. The days required for presence of hair, incisor eruption and testicular descent were statistically delayed in 1.0 mg/kg F1 rats. 6. The latency time for olfactory orientation was prolonged in 1.0 mg/kg F1 rats on postnatal day 15. Further, the positive response rates for air righting were reduced in 1.0 mg/kg F1 rats from postnatal day 17 to day 20. 7. 1.0 mg/kg paclitaxel caused suppression of the body weight gains in male F1 rats from postnatal week 1 to week 12. Though body weight gains were decreased in 1.0 mg/kg female F1 rats from postnatal week 1 to week 7, there were no significant differences between dosed animals and saline-treated animals regarding the body weight gains and food consumption during the gestation period. 8. Paclitaxel did not affect the learning ability and memory, spontaneous motor activity or emotionality in F1 rats. 9. The reproductive performance in both male and female F1 rats were not affected by paclitaxel. 10. Splenic weights were reduced in 1.0 mg/kg male and female F1 rats at weaning. Furthermore, liver weights were decreased in 1.0 mg/kg male F1 rats after mating. 11. No influence on prenatal development was observed for F2 fetuses even at the highest dose level of paclitaxel. Based on the reproductive and developmental indices, the no toxic-effect dose level of paclitaxel is 1.0 mg/kg/day and 0.3 mg/kg/day for dams (F0) and their offspring (F1), respectively.

Animals↗

PubMed1994

[Personal identification of a skull by a complete denture--application of superimposition and X-ray computed tomography analysis].

Personal identification from dentures is one of the main method used in the field of forensic odontology. When examining whether a denture left at investigation scene belongs to an unknown set of skeletal remains, it is not difficult to establish identity between the partial denture and the jaw of the skull by comparison of the number and kind of defective teeth and the degree of fit of the retainer to the abutment teeth. However, it is more troublesome to demonstrate identity between a complete denture and a skull since the morphological characteristics of the denture base, including the arrangement of the artificial teeth, have to be compared with those of the surfaces of the jawbones, which cannot be observed from the outside. In cases like this, superimposition and X-ray computed tomography are effective for establishing proof of identity. The method of superimposition involves taking an impression of the upper jaw to prepare a plaster model. A photograph of the denture applied to the plaster model is then taken and another photograph of the plaster model is obtained after removal of the denture under the same conditions. The former photo is printed on a transparent film and then superimposed on the latter. This method is useful for comparison between the arrangement of artificial teeth and the crest of the alveolar ridge, and between the shape of the polished (external) surface of the denture and the shape of the alveolar ridge.(ABSTRACT TRUNCATED AT 250 WORDS)

Cephalometry↗

PubMed1994

Absorbed doses modified by exposure settings with rotational panoramic radiography.

Absorbed doses to parotid gland, cervical vertebrae and thyroid gland were measured with various exposure settings of the tube voltage and tube current during use of rotational panoramic radiographs , Veraview (J. Morita Corp., Kyoto, Japan) and PM 2002 CC (Planmeca Oy, Helsinki, Finland) using Rando phantom and thermoluminescence dosimeters. The magnitude of each absorbed dose was greatest in the parotid gland, followed in order by the cervical vertebrae and thyroid gland and was proportional to tube current. The increase of the thyroid gland absorbed dose with increasing tube voltage was steeper than were the increases in the absorbed doses in either the parotid gland or the cervical vertebrae.

Absorption↗

PubMed1994

Absorbed dose reduced by sliced exposure using sector selector system with rotational panoramic radiography.

Sliced exposures which restrict the exposed area and adjust the region of diagnosis are possible using a sector selector system which is installed in the rotational panoramic radiograph, PM 2002 CC (Planmeca Oy, Helsinki, Finland). The effects of altering the horizontal and vertical sector selector system on thyroid gland dose were measured using Rando Phantom and thermoluminescence dosimeters. The horizontal sector selector system clarified that the most of the thyroid gland dose was delivered from exposure of the mandibular region. The vertical sector selector system clarified that scattered x-rays which originated from primary x-rays curved at the incisor and canine, premolar and molar, and ramus regions contributed equally to the thyroid gland dose.

Humans↗

PubMed1993

Absorbed doses with intraoral radiography. Function of various technical parameters.

Both the maxillary incisor and mandibular molar regions of a Rando phantom were radiographed using specific technical parameters. The parotid gland and thyroid gland doses were measured using thermoluminescence dosimetry. Tube voltage, total filtration, mAs, collimation, and cone length were varied while keeping the other factors constant. Increased tube voltage or total filtration generally resulted in a slightly increased absorbed dose. Absorbed dose was decreased as the beam was more tightly collimated or the cone length was increased.

Absorption↗

PubMed1993

Dose reduction to the thyroid gland in intraoral source radiography.

Organs near the oral cavity may be exposed to unacceptably high levels of radiation in intraoral source radiography. Three methods for reducing the dose to the thyroid gland have been investigated. A restriction of the exposure geometry by use of the B and C types of applicator was an efficient means of dose reduction. Absorbed doses to the thyroid gland were 30-50% less than those obtained when using the A-type applicator. A 10 degree upward inclination of the occlusal plane reduced the dose by 20%. In contrast, three thyroid protectors had no effect on dose reduction.

Absorption↗

PubMed1993

Diagnostic image quality and dose reduction using niobium filtration for cephalometric radiography.

The usefulness of added filtration is contingent upon the maintenance of high image quality while effectively reducing the radiation dose. High-quality cephalometric radiographs, defined objectively by a panel of orthodontists, were obtained of a tissue-equivalent phantom both without and with 30 microns and 50 microns supplemental niobium filtration. Skin entrance doses in the temporomandibular joint region were compared at diagnostically acceptable exposures where there was no significant difference in radiographic image quality. Dose reductions ranged from 17% to 31% with 30 microns niobium filtration, and 17% to 39% with 50 microns. However, as kVp and HVL were increased to maintain the same level of perceived image quality, image contrast decreased. Contrast loss with 30 microns and 50 microns niobium filtration averaged 8% and 22% respectively compared with standard aluminium filtration. It would appear that additional niobium filtration can be used to reduce radiation dose without significant loss of image quality.

Analysis of Variance↗

PubMed1993

Morphometric analysis of image distortion with rotational panoramic radiography.

We studied the image distortion of spherical objects that occurred during rotational panoramic radiography. We measured the magnification of height, width, perimeter and area, and calculated the circularity index to quantify the distortion. Spherical objects of different diameters were projected on the film. A complex relationship was found between the position and the horizontal magnification for each anatomical region. The object's size influenced the horizontal magnification to a considerable extent. The greatest changes in circularity occurred at the midline. This morphological distortion was caused by non-symmetrical projection during the scanning of these spherical objects, which resulted from the continuous changes in the scanning velocity and the position of the x-ray beam. This result suggests a complex distortion of the inner structures of macroscopic objects.

Humans↗

PubMed1992

Comparison of spatial resolution between tomographic images and ghosts in rotational panoramic radiography: the effect of the first slit width.

Rotational panoramic radiography projects the object placed within the focal trough as tomographic images with minimal distortion. However, even if an object is within the focal trough, the sharpness of the image is affected by the position of the object, the difference in the relative speed of the X-ray source against the film and the difference in the direction of rotation. In this study, spatial resolutions of ghosts and tomographic images were compared. Ghosts, as well as tomographic images, were obtained from the object placed in the focal trough. The effects of the width of the first slit, a major parameter affecting image quality, were the primary concern in this study. With regard to ghosts, the influence of the position of object, in terms of central, buccal and lingual regions the focal trough, was also analyzed. The target of analysis was the mandibular ramus region. An Orthopantomograph OP5, a conventional panoramic apparatus, was used. An X-ray test chart, X-ray beams, and H-D curve were prepared for calculation of the Modulation Transfer Function (MTF), and the slit method using the Fourier transforms was applied. When the width of the first slit was changed displacement of MTF curves was most remarkable for the superimposed tomographic image which was scanned 10 mm posterior to the X-axis connecting the right and left lateral rotation centers. For reduction of the spatial resolution of ghosts, it was found effective to use a wider slit and place the object at a position lingual to the central plane.

Artifacts↗

PubMed1992

[Cefepime (diHCl/L-arginine blend): intravenous continuous infusion and/or single dose subcutaneous toxicity study in rats and dogs].

To investigate single dose toxicity of cefepime (CFPM diHCl/L-arginine blend), the test drug was administered to rats [Crj: CD (SD)] of both sexes at dose levels of 500, 1,000 and 2,000 mg/kg using intravenous continuous infusion or subcutaneous injection, and to male beagle dogs at 1,000 and 2,000 mg/kg using intravenous continuous infusion. As the control, two additional groups of each animals were given either saline or L-arginine alone which was used in the test formulation to adjust pH values of CFPM diHCl solutions. The obtained results are summarized as follows: 1. Rats dosed with 2,000 mg/kg CFPM through intravenous continuous infusion showed slightly decreased spontaneous physical activity. One male rat dosed with L-arginine alone via continuous infusion also showed slightly decreased activity. Slight to severe inflammatory reactions at injection sites including sloughing of the tail were prominent at doses of 1,000 or 2,000 mg/kg of CFPM, or L-arginine alone. Average body weights of rats in the test groups of either sex were comparable to the controls in all of the dose groups of the same sex during the 14-day test period. 2. Rats receiving 2,000 mg/kg CFPM in single subcutaneous injection showed slightly diminished activities. Slight to moderate reactions occurred around the injection site (viz., hardening, depilation, scab-formation and necrosis) in rats injected any of the 3 doses of CFPM. Though body weight gains were slightly retarded in male rats receiving 2,000 mg/kg CFPM during the last half of the observation period, such weight gain retardation was not observed in rats of other dose groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1992

[Cefepime (BMY-28142 diHCl/L-arginine blend): one-month repeated dose subcutaneous toxicity study in rats].

In order to investigate the toxicity of cefepime (CFPM, BMY-28142 diHCl/L-arginine blend upon repeated subcutaneous dosing), the test article was administered to Crj:CD(SD) rats of both sexes at daily dose levels of 150 (low dose), 500 (intermediate dose) and 1,500 (high dose) mg/kg/day by subcutaneous route for 28 days. Two additional groups of rats were given either saline (negative control) or L-arginine (vehicle control). Doses were equally divided and administered twice each day with an interval of approximately 5 hours between the 2 doses of a same day. A half of rats in negative control and high dose groups were retained for examination during one-month recovery period. The results obtained are summarized as follows: 1. Upon general observations, it was found that drug-related changes were restricted to the injection sites. Depilation and scab-formation of the injection sites were noted in high dose rats of both sexes and intermediate dose females. No deaths occurred during the study. 2. Slightly depressed body weight gains were observed for high dose males during the latter part of the dosing period. 3. Slightly lower food consumptions were noted for intermediate and high dose males at Week 1. 4. Slightly higher water consumptions were generally detected for high dose rats during the dosing period. 5. Hematological examinations revealed that a slight decrease in the average value of relative lymphocyte counts and a slight increase in the average value of relative segmented neutrophil counts were evident for high dose males. These findings might be attributable to the inflammatory reactions at the injection sites.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1992

[Reproductive and developmental toxicity studies on cefepime dihydrochloride administered subcutaneously to rats during the premating, gestation and lactation periods].

Cefepime dihydrochloride (CFPM) was administered subcutaneously daily at doses of 0, 150, 500 and 1,000 mg/kg for 63 days prior to mating and during mating to male Crj: CD (SD) rats and for 14 days prior to mating and during mating, as well as periods of gestation and lactation to female SD rats. Saline and L-arginine hydrochloride (L-arginine) were used as control articles. Daily doses of test and control articles were equally divided and administered twice a day (b.i.d.). The results obtained are summarized as follows: 1. Soft stool was observed for both male and female F0 rats at CFPM 1,000 mg/kg at the first week of administration period. Further, depilation of injection sites was found in 7 males and 12 females at the same dose level. 2. Body weight gains were suppressed in male F0 rats from Day 28 to 63 of administration period at CFPM 1,000 mg/kg. Moreover, food consumption was reduced in F0 female rats during the first week of administration period at all dose levels of CFPM. 3. CFPM failed to affect the reproductive performance in both male and female F0 rats. 4. Kidney weights were increased in both male and female F0 rats and adrenal weights were augmented in male F0 rats at CFPM 1,000 mg/kg. On the other hand, cecal enlargement were observed for F0 dams treated with CFPM. However, these changes were not considered to be unique to this drug, because they have been described with most antibiotics in this species and appears to be results of modifications in gut flora. 5. Prenatal developments in F1 fetuses were not affected by CFPM. 6. CFPM failed to affect delivery status of F0 dams or survival and lactation indices in F1 pups. 7. CFPM did not affect postnatal differentiations, developmental behaviors, learning ability and memory, spontaneous motor activity or emotionality in F1 rats. 8. Body weight gains and food consumption in both male and female F1 rats were not affected by CFPM. 9. CFPM did not alter the organ weights in both male and female F1 rats. 10. There were no significant differences between drug treated animals and controls regarding the reproductive performance and delivery status of F1 rats. 11. Influences on survival indices, body weights and organ weights were not apparently observed for F2 pups even at CFPM 1,000 mg/kg. Based on the reproductive and developmental indices, the no-effect dose level of CFPM under the present experimental condition was estimated to be 1,000 mg/kg/day against dams (F0) and their offspring (F1).

Animals↗

PubMed1991

Redundant shadows in rotational panoramic radiographs. Estimating the imaging positions of objects by mathematical analysis.

The sources of real, ghost, and double images in rotational panoramic radiographs generated by a machine with a continuous movement of the center of rotation are described. No assumptions are made with regard to the trajectory of the center of rotation; rather, an empirical approximation scheme is used to establish a regression from which measured positions of reference objects can be predicted.

Artifacts↗

PubMed1991

Mathematical approach to horizontal and vertical magnification factors in rotational panoramic radiography--with attention to redundant shadows.

Rotational panoramic radiographs consist of two different images, the tomographic image and redundant shadows. Many authors have reported magnification factors for the tomographic image which include the term "real image", as proposed by McDavid et al. in 1983. However, a mathematical formula has never been proposed for ghost images, which redundant shadows projected only when the object is located between the center of rotation and the X-ray source. The purpose of this study was to propose a new mathematical approach for calculating the horizontal and the vertical magnification factors for ghost images. Using this approach, we investigated the effect of variations in the position of the object, its length, and its placement angle with respect to the X-ray beam. Since the object was assumed to be small, an approximated expression for film speed was integrated to determine the length of the object on the film in the horizontal dimension. The approximated and the geometric expressions proposed by Wakoh in 1988 were used to calculate the vertical dimension. Differences between the scanning and the film speeds were assumed to affect the horizontal magnification factors of the ghost image. In addition, differences in the setting position, length, and angle of the object affected the calculated values. Vertical magnification factors were considerably influenced by the Z coordinate of the position, even though the length and angle remained constant.

Artifacts↗

PubMed1990

[Acute toxicity study of buspirone hydrochloride in mice, rats and dogs].

Buspirone hydrochloride (abbr. to BH), an anxiolytic drug, was examined for its intravenous, subcutaneous or oral acute toxicity using Crj: CD-1 (ICR) mice, Crj: CD (Sprague-Dawley) rats and beagle dogs of both sexes. The results obtained were summarized as follows: 1. Drug-related toxic signs included decreased activity and convulsions accompanied with salivation and opisthotonus in mice and rats treated with BH regardless of administration routes, and tremors and clonic convulsions accompanied with salivation in dogs treated with BH orally. 2. Pathological examinations revealed distention of the stomach in dead rats treated with BH orally, and hypersecretion of gastric juice and alterations (viz. edema, necrosis and petechia) on the superficial mucous membrane in the gastropyloric region in dead dogs treated with BH orally. 3. The cause of death was considered to be due to respiratory insufficiency in every species of animals examined. 4. LD50 values (mg/kg) were as follows: [table: see text] 5. No sex differences were observed in every species of animals regardless of administration routes on the basis of toxicological parameters examined.

Administration, Oral↗

PubMed1990

[Reproductive and developmental toxicity studies of buspirone hydrochloride (I)--Oral administration to rats during the period of fetal organogenesis].

Buspirone hydrochloride (buspirone), an anxiolytic drug, was administered orally to pregnant Crj: CD (Sprague-Dawley) rats from day 7 through 17 of gestation at dose levels of 2, 12 and 75 mg/kg/day. The summarized results obtained are as follows: 1. Decreased activity was observed for F0 dams at buspirone 75 mg/kg. Further, the suppression of maternal body weight gains accompanied by the reduction of food consumption was shown during the administration period at the same dose level. 2. Liver weights were increased in F0 dams at term at buspirone 12 mg/kg and higher. Besides, brain, pituitary, adrenal and ovarian weights were increased in F0 dams at term at buspirone 75 mg/kg. 3. Buspirone 75 mg/kg brought the inhibition of fetal growth followed by the lowered values in fetal weights, crown-rump distances and tail lengths. Furthermore, the elevated incidence of skeletal abnormalities such as nodular and wavy ribs and unossified 5th and 6th sternum , as well as retarded ossification of cervical vertebrae, forelimbs and hindlimbs were also noted in this dose level. Also, the retarded ossification was observed at 12 mg/kg. 4. Buspirone failed to affect the parturition of F0 dams. 5. Buspirone did not function the viability of newborns (F1), and postnatal differentiations, learning ability, motility, motor activity or emotional development in F1 animals. 6. Body weight gains were depressed in female F1 rats from 4 to 9 weeks of age and food consumption was decreased in male F1 rats from 6 to 8 weeks of age at buspirone 75 mg/kg. 7. Buspirone 75 mg/kg produced suppressions of brain weights at 10 weeks of age in male and female F1 rats and lung weights at weaning in male F1 rats. Spleen weights were increased in female F1 rats at 10 weeks of age at the same dose level. However, buspirone failed to affect their reproductive ability. 8. F2 neonates derived from F1 rats whose dams had ever received buspirone during the period of fetal organogenesis showed no changes in observation items at birth. Based on these results, the no-effect dose level of oral buspirone under the present experimental condition was estimated to be 2 mg/kg/day against dams and their offspring.

Abnormalities, Drug-Induced↗

PubMed1990

[Reproductive and developmental toxicity studies of buspirone hydrochloride (II)--Oral administration to rats during perinatal and lactation periods].

Buspirone hydrochloride (buspirone), an anxiolytic drug, was administered orally to pregnant Crj: CD (Sprague-Dawley) rats from day 17 of gestation through day 20 of postpartum at dose levels of 2, 12 and 75 mg/kg/day. The summarized results obtained are as follows: 1. Decreased activity was observed for F0 dams at buspirone 75 mg/kg. Further, the suppression of maternal body weight gains accompanied by the reduction of food consumption was shown during the administration period at buspirone 12 mg/kg and higher. 2. Brain and adrenal weights were increased in F0 dams at buspirone 12 mg/kg and higher. Besides, lung and pituitary weights were augmented in F0 dams at buspirone 75 mg/kg. 3. Buspirone 75 mg/kg brought the increased number of stillbirths in F1 neonates. 4. Buspirone 75 mg/kg lowered the viability of newborns (F1) on postnatal day 3 and prolonged the days required for pinnae detachment, presence of abdominal hair and eye opening in offspring (F1), but failed to function their learning ability, motility, motor activity or emotional development. 5. Body weight gains were depressed in both male and female F1 rats at buspirone 12 mg/kg and higher. Food consumption was also decreased in both sexes at the same dose levels. 6. Heart weights were decreased in female F1 rats after mating at buspirone 12 mg/kg and higher. Further, buspirone 75 mg/kg brought a suppression of brain weights at 10 weeks of age in male and female F1 rats, but failed to affect their reproductive ability. 7. F2 neonates derived from F1 rats whose dams had ever received buspirone during the perinatal and lactation periods showed no changes in observation items at birth. Based on these results, the no-effect dose level of oral buspirone under the present experimental condition was estimated to be 2 mg/kg/day against dams and their offspring.

Abnormalities, Drug-Induced↗

PubMed1990

New approach to analysis of rotational panoramic radiography by computer simulation.

Computer simulation is an effective method for mathematical analysis of the image of rotational panoramic radiography. Conventional simulation methods have been used only for analysis of imaging factors and prediction of the characteristics of the obtained image. This paper describes a new computer simulation method that analyzes the imaging factors and produces the simulated image itself. The simulation is carried out by reproducing the actual image formation process in the computer. The head phantom for simulation, which represents the human dentomaxillofacial structures as a numerical value from 0 to 4, was constructed by scanning a real head phantom from neck to head with an X-ray CT scanner at a slice thickness of 1 mm and at 1 mm slice intervals. The diverging X-ray beam was expressed as a set of straight lines and was moved according to various parameters. The simulated image obtained was satisfactory for image analysis of rotational panoramic radiography.

Computer Simulation↗